公开(公告)号：US20090311380A1

公开(公告)日：2009-12-17

申请号：US12466072

申请日：2009-05-14

申请人： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  James John Ragghianti ,  John E. Seip ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

发明人： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  James John Ragghianti ,  John E. Seip ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC分类号： A23L1/30 ,  C12N1/19 ,  C07C57/03 ,  A23L1/28 ,  A23C9/12 ,  A23K1/16

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。

公开(公告)号：US20090274816A1

公开(公告)日：2009-11-05

申请号：US12420345

申请日：2009-04-08

申请人： Howard Glenn Damude ,  Narendra S. Yadav

发明人： Howard Glenn Damude ,  Narendra S. Yadav

IPC分类号： A23K1/16 ,  C12N15/74 ,  A01H5/00 ,  C11B1/00 ,  A01H1/00

CPC分类号： C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

摘要翻译： 本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌Δ-15脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。 本文还描述了通过过表达Δ-15脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。

公开(公告)号：US07556949B2

公开(公告)日：2009-07-07

申请号：US11779915

申请日：2007-07-19

申请人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

发明人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC分类号： C12N1/16 ,  C12P7/64 ,  C12N9/02 ,  C07H21/04

CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6472

摘要： The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.

摘要翻译： 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物以及使用这些Delta17去饱和酶在含油酵母中制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090104674A1

公开(公告)日：2009-04-23

申请号：US12338536

申请日：2008-12-18

申请人： Narendra S. Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

发明人： Narendra S. Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

IPC分类号： C12P7/64

CPC分类号： C12N9/1029 ,  C12P7/6463 ,  C12P7/6472

摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。

公开(公告)号：US07465565B2

公开(公告)日：2008-12-16

申请号：US11592806

申请日：2006-11-03

申请人： Narendra S. Yadav ,  Hongxiang Zhang

发明人： Narendra S. Yadav ,  Hongxiang Zhang

IPC分类号： C12Q1/48 ,  C12P7/64 ,  C12P21/06 ,  C12N9/10 ,  C12N1/16 ,  C12N15/70 ,  C07H21/04

CPC分类号： C12P7/6481 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

摘要： Two acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, the genes encoding phophatidylcholine-diacylglycerol acyltransferase (PDAT) and diacylglycerol acyltransferase (DGAT2) have been isolated from Y. lipolytica. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

摘要翻译： 提供两种酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω-脂肪酸的微生物油。 具体地说，从解脂耶氏酵母中分离出编码磷脂酰胆碱 - 二酰基甘油酰基转移酶（PDAT）和二酰基甘油酰基转移酶（DGAT2）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。

公开(公告)号：US07238482B2

公开(公告)日：2007-07-03

申请号：US10840579

申请日：2004-05-06

申请人： Stephen K. Picataggio ,  Narendra S. Yadav ,  Quinn Qun Zhu

发明人： Stephen K. Picataggio ,  Narendra S. Yadav ,  Quinn Qun Zhu

IPC分类号： C12Q1/68 ,  C12N1/19 ,  C07H21/04 ,  C12N15/63

CPC分类号： C12N15/815 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.

公开(公告)号：US6077992A

公开(公告)日：2000-06-20

申请号：US178089

申请日：1998-10-23

申请人： Narendra S. Yadav

发明人： Narendra S. Yadav

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  C12N15/90

CPC分类号： C12N15/8217 ,  C12N15/8203

摘要： This invention relates to a regulated binary plant viral expression system. It is comprised of two chromosomally-integrated components. One component is a proreplicon, which contains cis-acting viral sequences required for replication and a contains a target gene. The other component is a chimeric trans-acting replication gene comprising a regulated promoter operably-linked to the coding region for a viral replication protein. The proreplicon lacks the replication gene essential for replicon replication, and thus cannot undergo autonomous episomal replication. However, regulated expression of the trans-acting replication protein in plant cells also containing the proreplicon will trigger the release of free replicon from the integrated proreplicon, result in its episomal replication in trans, and result in the expression of the target gene, if present, through gene amplification. The expression system is useful for both production of foreign proteins as well as silencing endogenous genes and transgenes in plant tissue. Tissue-specific expression is controlled by the choice of promoter controlling the transcription of the trans-acting replication gene.

摘要翻译： 本发明涉及调节二元植物病毒表达系统。 它由两个染色体整合的组件组成。 一个组分是一个proreplicon，其包含复制所需的顺式作用病毒序列和含有靶基因。 另一组分是嵌合反式作用复制基因，其包含可操作地连接于病毒复制蛋白的编码区的调节启动子。 复制子缺乏复制子复制必需的复制基因，因此不能进行自发性增殖复制。 然而，也含有副反应蛋白的植物细胞中的反式作用复制蛋白的调控表达将触发从整合的复制子释放游离的复制子，导致其反式的附加型复制，并导致靶基因的表达（如果存在） ，通过基因扩增。 表达系统对于外源蛋白的生产以及沉默植物组织中的内源基因和转基因是有用的。 组织特异性表达是通过控制反式作用复制基因转录的启动子的选择来控制的。

公开(公告)号：US5378824A

公开(公告)日：1995-01-03

申请号：US892305

申请日：1992-06-02

申请人： John R. Bedbrook ,  Roy S. Chaleff ,  Saverio C. Falco ,  Barbara J. Mazur ,  Christopher R. Somerville ,  Narendra S. Yadav

发明人： John R. Bedbrook ,  Roy S. Chaleff ,  Saverio C. Falco ,  Barbara J. Mazur ,  Christopher R. Somerville ,  Narendra S. Yadav

IPC分类号： C12N9/88 ,  C12N15/82 ,  C07H21/04 ,  C12N15/00 ,  C12P21/00 ,  C12P21/04

CPC分类号： C12N15/8274 ,  C12N15/8278 ,  C12N9/88

摘要： A nucleic acid fragment encoding a herbicide-resistant plant acetolactate synthase protein is disclosed. This nucleic acid fragment contains at least one nucleotide mutation resulting in one amino acid change in one of seven substantially conserved regions of acetolactate synthase amino acid homology. This mutation results in the production of an acetolactate synthase protein which is resistant to sulfonylurea herbicide compounds compared to the wild-type protein. Transformation of herbicide sensitive plants or plant cells with the fragment results in resistance to the herbicide.

摘要翻译： 公开了编码除草剂抗性植物乙酰乳酸合酶蛋白的核酸片段。 该核酸片段含有至少一个核苷酸突变，导致乙酰乳酸合酶氨基酸同源性的七个基本上保守的区域之一发生一个氨基酸变化。 与野生型蛋白质相比，该突变导致对磺酰脲类除草剂化合物具有抗性的乙酰乳酸合酶蛋白质的产生。 用片段转化除草剂敏感植物或植物细胞导致对除草剂的抗性。

公开(公告)号：US09057083B2

公开(公告)日：2015-06-16

申请号：US12396845

申请日：2009-03-03

申请人： Narendra S. Yadav ,  Hongxiang Zhang

发明人： Narendra S. Yadav ,  Hongxiang Zhang

IPC分类号： C12N9/02 ,  C12P7/64 ,  C12Q1/26 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12P7/6427 ,  C12N9/001 ,  C12N9/0083 ,  C12P7/6472 ,  C12Q1/6895

摘要： The present invention relates to fungal Δ12 desaturases (responsible for conversion of oleic acid to linoleic acid (18:2, LA)) and Δ15 fatty acid desaturases (responsible for conversion of LA to α-linolenic acid (18:3, ALA)). Amino acid motifs diagnostic of Δ12 desaturases and Δ15 desaturases are also provided. Methods of altering enzyme specificity towards Δ12 desaturation or towards Δ15 desaturation and/or increasing production of specific ω-3 and ω-6 fatty acids by over-expression of the fungal desaturases are also described.

摘要翻译： 本发明涉及真菌和Dgr 12脱氢酶（负责将油酸转化为亚油酸（18：2，LA））和＆Dgr.15脂肪酸去饱和酶（负责将LA转化成α-亚麻酸（18：3 ，ALA））。 还提供了＆Dgr; 12去饱和酶和＆Dgr。15去饱和酶的氨基酸图案诊断。 还描述了通过过度表达真菌去饱和酶改变酶特异性的方法来改变＆Dgr; 12去饱和或朝向＆Dgr; 15去饱和和/或增加特定ω-3和ω-6脂肪酸的产生。

公开(公告)号：US08518674B2

公开(公告)日：2013-08-27

申请号：US12893469

申请日：2010-09-29

申请人： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

发明人： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC分类号： C12P7/64 ,  C12N1/00 ,  A01N37/00 ,  A23D9/00

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。