公开(公告)号：US20050053913A1

公开(公告)日：2005-03-10

申请号：US10921581

申请日：2004-08-18

Applicant: Marc Vidal ,  Jef Boeke ,  Ed Harlow

Inventor： Marc Vidal ,  Jef Boeke ,  Ed Harlow

IPC: C12N15/09 ,  C12N1/19 ,  C12N15/10 ,  C12Q1/68 ,  C12R1/865 ,  G01N33/50 ,  G01N33/53 ,  C12N1/18 ,  C12N15/74

CPC classification number: G01N33/5023 ,  C12N15/1055 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/505 ,  G01N33/53 ,  G01N2333/39 ,  C12Q2565/201 ,  C12Q2522/101

Abstract: Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

Abstract translation: 公开了用于鉴定分子相互作用（例如蛋白质/蛋白质，蛋白质/ DNA，蛋白质/ RNA或RNA / RNA相互作用）的方法。 本发明中的所有方法都采用反选择和至少两种杂交分子。 相互作用的分子重构转录因子并直接表达报告基因，然后测定其表达。 还公开了可用于实践本发明的方法的遗传构建体。

公开(公告)号：US20040146931A1

公开(公告)日：2004-07-29

申请号：US10801994

申请日：2004-03-16

Applicant: Massachusetts Institute of Technology

Inventor： J. Keith Joung ,  Jeffrey Miller ,  Carl O. Pabo

IPC: C12Q001/68 ,  G01N033/554 ,  G01N033/569 ,  C12N015/74

CPC classification number: G01N33/502 ,  C12N15/1055 ,  C12Q1/6897 ,  G01N33/50 ,  G01N33/5008 ,  C12Q2565/201

Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.

Abstract translation: 本发明提供了用于检测蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的相互作用阱测定法的方法和组合物。 本发明的方法和组合物还可用于鉴定可能激动或拮抗蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的试剂。 在某些实施方案中，本发明的相互作用捕获系统可用于筛选具有大于10 7个成员的文库。 在其它实施方案中，本发明的相互作用阱系统结合流式细胞术使用。 本发明还提供了用于同时筛选靶蛋白或核酸序列以获得与两种或多种测试蛋白或核酸相互作用的能力的方法。

公开(公告)号：US20040038249A1

公开(公告)日：2004-02-26

申请号：US10366715

申请日：2003-02-14

Inventor： Raphael Darteil ,  Vincent Thuillier

IPC: C12Q001/68 ,  C07H021/04 ,  C07K014/705 ,  C12N015/86

CPC classification number: C12Q1/6897 ,  C07K14/70567 ,  C12N15/85 ,  C12N2830/001 ,  C12N2830/42 ,  C12Q2565/201

Abstract: The invention relates to novel mammalian perixosome proliferator-activated receptor (PPAR) poplypeptides and their use. In a particular embodiment, PPAR polypeptides with mutations in the P-box domain possess advantageous properties as transcriptional activators for PPRE-bearing expression vectors and expression systems. The invention includes PPAR polypeptides, nucleic acids encoding them, expression systems, vectors, and methods for inducibly expressing a gene of interest. The methods and expression systems of the invetion provide improved dose-response or inducibility characteristics, improved and/or altered effects on cellular PPAR function, and/or improved transcriptional control. The selection of a homologous PPAR polypeptide to prepare the novel PPAR polypeptide of the invetion for a cell or tissue also improves the immune reaction side effect potential for particular uses.

Abstract translation: 本发明涉及新型哺乳动物细胞增生子激活受体（PPAR）多肽及其用途。 在一个具体实施方案中，在P盒结构域中具有突变的PPAR多肽具有作为携带PPRE的表达载体和表达系统的转录激活子的有利特征。 本发明包括PPAR多肽，编码它们的核酸，表达系统，载体和用于诱导表达感兴趣的基因的方法。 投药的方法和表达系统提供改善的剂量反应或诱导性特征，改善和/或改变对细胞PPAR功能的影响和/或改进的转录控制。 同源PPAR多肽的选择以制备对细胞或组织的侵袭的新型PPAR多肽也改善了特定用途的免疫反应副作用潜力。

公开(公告)号：US20030219817A1

公开(公告)日：2003-11-27

申请号：US10423495

申请日：2003-04-24

Inventor： Li Zhu ,  Shaobing B. Hua ,  James Sheridan ,  Yu-Huei Lin

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/569 ,  C12N001/18 ,  C12N015/74

CPC classification number: C40B30/04 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/55 ,  C12Q1/6897 ,  G01N33/6845 ,  C12Q2565/201

Abstract: Compositions, methods, and kits are provided for efficiently generating and screening a library of highly diverse protein complexes for their ability to bind to other proteins or oligonucleotide sequences. In one aspect of the invention, a library of expression vectors is provided for expressing the library of protein complexes. The library comprises a first nucleotide sequence encoding a first polypeptide subunit; and a second nucleotide sequence encoding a second polypeptide subunit. The first and second nucleotide sequences each independently vary within the library of expression vectors. In addition, the first and second polypeptide subunit are expressed as separate proteins which self-assemble to form a protein complex, such as a double-chain antibody fragment (dcFv or Fab) and a fully assembled antibody, in cells into which the library of expression vectors are introduced. The library of expression vectors can be efficiently generated in yeast cells through homologous recombination; and the encoded proteins complexes with high binding affinity to their target molecule can be selected by high throughput screening in vivo or in vitro.

Abstract translation: 提供了组合物，方法和试剂盒，用于有效地产生和筛选高度多样化的蛋白质复合物的文库以获得与其它蛋白质或寡核苷酸序列结合的能力。 在本发明的一个方面，提供表达载体文库用于表达蛋白质复合物的文库。 该文库包含编码第一多肽亚基的第一核苷酸序列; 和编码第二多肽亚基的第二核苷酸序列。 第一和第二核苷酸序列各自独立地在表达载体的文库内变化。 此外，第一和第二多肽亚基表达为分离的蛋白，其自组装形成蛋白复合物，例如双链抗体片段（dcFv或Fab）和完全组装的抗体，其中文库 引入表达载体。 表达载体文库可以通过同源重组在酵母细胞中有效产生; 并且可以通过体内或体外的高通量筛选来选择对其靶分子具有高结合亲和力的编码蛋白复合物。

公开(公告)号：US20030186317A1

公开(公告)日：2003-10-02

申请号：US09971782

申请日：2001-10-09

Applicant: Myriad Genetics, Inc.

Inventor： Jean-Marc Roch ,  Paul L. Bartel ,  Karen Heichman

IPC: G01N033/53 ,  G01N033/542

CPC classification number: G01N33/6896 ,  A61K38/1709 ,  C07K14/47 ,  C07K14/4711 ,  C07K16/18 ,  C07K16/2833 ,  C07K2317/32 ,  C12Q1/6883 ,  C12Q1/6897 ,  C12Q2600/156 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/28 ,  C12Q2565/201

Abstract: The present invention relates to the discovery of protein-protein interactions that are involved in the pathogenesis of neurodegenerative disorders, including Alzheimer's disease (AD). Thus, the present invention is directed to complexes of these proteins and/or their fragments, antibodies to the complexes, diagnosis of neurodegenerative disorders (including diagnosis of a predisposition to and diagnosis of the existence of the disorder), drug screening for agents which modulate the interaction of proteins described herein, and identification of additional proteins in the pathway common to the proteins described herein.

公开(公告)号：US20030013110A1

公开(公告)日：2003-01-16

申请号：US10176826

申请日：2002-06-20

Inventor： Markus Kurz ,  Peter Lohse

IPC: C12Q001/68 ,  C07K014/00

CPC classification number: C12Q1/6897 ,  C12N15/1055 ,  C12N15/1062 ,  C12N15/1075 ,  C12Q2565/201 ,  C12Q2525/203

Abstract: Disclosed herein are in vitro assays for the identification of interactions between proteins or other molecules, the identification of transcriptional activator proteins, and the detection of compounds that inhibit protein/protein or protein/compound interactions. Also disclosed herein are in vitro assays for the selection of interacting proteins and transcriptional activator proteins out of libraries.

Abstract translation: 本文公开了用于鉴定蛋白质或其他分子之间的相互作用的体外测定，转录激活蛋白的鉴定以及抑制蛋白质/蛋白质或蛋白质/化合物相互作用的化合物的检测。 本文还公开了用于从文库中选择相互作用蛋白和转录激活蛋白的体外测定。

公开(公告)号：US20030007983A1

公开(公告)日：2003-01-09

申请号：US10006626

申请日：2001-12-06

Inventor： Stephen P. Goff ,  Gilda Tachedjian ,  Bryan Mark O'Hara

IPC: C12Q001/70 ,  C12P021/06 ,  C12P019/34 ,  A61K039/00 ,  A61K039/38 ,  A61K039/21

CPC classification number: C12Y207/07049 ,  C07K2319/00 ,  C12N9/1276 ,  C12Q1/6897 ,  C12Q2565/201

Abstract: This invention provides methods of determining whether a compound inhibits HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound inhibits formation of a complex between a p66 and p51 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides a method of determining whether a compound enhances formation of a complex between a p66 and p51 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound inhibits formation of a complex between two p66 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound enhances formation of a complex between two p66 subunit polypeptides of HIV-1 reverse transcriptase.

Abstract translation: 本发明提供了确定化合物是否抑制HIV-1逆转录酶的方法。 本发明提供了确定化合物是否抑制HIV-1逆转录酶的p66和p51亚基多肽之间复合物形成的方法。 本发明提供了确定化合物是否增强HIV-1逆转录酶的p66和p51亚基多肽之间的复合物形成的方法。 本发明提供了确定化合物是否抑制HIV-1逆转录酶的两个p66亚基多肽之间复合物形成的方法。 本发明提供了确定化合物是否增强HIV-1逆转录酶的两个p66亚基多肽之间复合物形成的方法。

公开(公告)号：US6114111A

公开(公告)日：2000-09-05

申请号：US50863

申请日：1998-03-30

Applicant: Ying Luo ,  Betty Huang ,  Donald Payan

Inventor： Ying Luo ,  Betty Huang ,  Donald Payan

IPC: C12N15/10 ,  C12N15/12 ,  C12Q1/70

CPC classification number: C12N15/1055 ,  C12Q2565/201

Abstract: The present invention is directed to compositions and methods for a genetic system of detecting protein--protein interactions in a mammalian host cell. Two fusion proteins are made in the host cell. The first fusion protein contains a DNA binding domain which is fused to a so-called bait protein. The second fusion protein consists of a transcriptional activation domain fused to a so-called test protein. The transcriptional activation domain is recruited to the promoter through the functional interaction between the bait protein and the test protein. Subsequently the transcriptional activation domain interacts with the basal transcription machinery to activate expression of one or more reporter genes which can be identified and characterized. The individual compositions are useful for analyzing protein--protein interactions between known proteins and to isolate, clone and characterize unknown proteins. The individual compositions can be used to express the fusion proteins either transiently or stably.

Abstract translation: 本发明涉及用于在哺乳动物宿主细胞中检测蛋白质 - 蛋白质相互作用的遗传系统的组合物和方法。 在宿主细胞中制备两种融合蛋白。 第一个融合蛋白含有与所谓诱饵蛋白融合的DNA结合结构域。 第二种融合蛋白由与所谓的测试蛋白融合的转录激活结构域组成。 通过诱饵蛋白质和测试蛋白质之间的功能相互作用将转录激活结构域引入启动子。 随后，转录激活结构域与基础转录机制相互作用以激活一种或多种可鉴定和表征的报告基因的表达。 单独的组合物可用于分析已知蛋白质之间的蛋白质 - 蛋白质相互作用并分离，克隆和表征未知蛋白质。 单独的组合物可用于瞬时或稳定地表达融合蛋白。

公开(公告)号：US08288107B2

公开(公告)日：2012-10-16

申请号：US11821812

申请日：2007-06-25

Applicant: John L. Teem

Inventor： John L. Teem

IPC: C12Q1/68 ,  C12N1/19 ,  C12N15/85

CPC classification number: G01N33/564 ,  C07K14/4712 ,  C07K2319/00 ,  C12N15/1055 ,  C12Q1/6895 ,  C12Q1/6897 ,  C12Q2565/201

Abstract: The subject invention concerns materials and methods for detecting the interaction of CFTR proteins. In one embodiment, the method can be used to determine whether one CFTR polypeptide interacts with a second CFTR polypeptide. The subject invention also concerns materials and methods for screening for drugs or compositions that can restore or enhance interaction of CFTR proteins containing mutation(s) that reduce or prevent dimerization of the proteins. The assay of the present invention can be used to screen a large number of compounds in a high throughput format. The subject invention also pertains to host cells useful in the methods of the invention. The subject invention also concerns compositions and methods for treating patients afflicted with cystic fibrosis.

Abstract translation: 本发明涉及用于检测CFTR蛋白质相互作用的材料和方法。 在一个实施方案中，该方法可用于确定一种CFTR多肽是否与第二CFTR多肽相互作用。 本发明还涉及用于筛选能够还原或增强含有减少或阻止蛋白质二聚化的突变的CFTR蛋白质相互作用的药物或组合物的材料和方法。 本发明的测定可用于以高通量形式筛选大量化合物。 本发明还涉及可用于本发明方法的宿主细胞。 本发明还涉及用于治疗患有囊性纤维化的患者的组合物和方法。

公开(公告)号：US07892772B2

公开(公告)日：2011-02-22

申请号：US11685122

申请日：2007-03-12

Applicant: John Roy Rohde ,  Claude Rene Raoul Parsot ,  Philippe Joseph Sansonetti

Inventor： John Roy Rohde ,  Claude Rene Raoul Parsot ,  Philippe Joseph Sansonetti

IPC: C12P21/04 ,  C12N15/11

CPC classification number: C12Y603/02019 ,  C12N9/93 ,  C12Q2565/201 ,  G01N33/5023

Abstract: Members of the IpaH superfamily constitute a novel class of E3 ubiquitin ligases which are useful for engineering products which modulate trafficking and destruction of target proteins inside a cell and useful targets for identifying new antimicrobial molecules which modulate, especially inhibit, E3 ligases.

Abstract translation: IpaH超家族的成员构成了E3泛素连接酶的新一类，其可用于调节细胞内靶蛋白的运输和破坏的工程产物，并且用于鉴定调节，特别是抑制E3连接酶的新抗微生物分子的有用靶标。