公开(公告)号：US11971341B2

公开(公告)日：2024-04-30

申请号：US16196479

申请日：2018-11-20

Applicant: MINITÜB GMBH

Inventor： Georg Wippenbeck ,  Christian Simmet

IPC: B01L3/00 ,  C12Q1/06 ,  G01N15/06 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N21/03 ,  G02B21/34 ,  G02B21/16

CPC classification number: G01N15/1404 ,  B01L3/5027 ,  C12Q1/06 ,  G01N15/06 ,  G02B21/34 ,  B01L2200/021 ,  B01L2200/026 ,  B01L2200/14 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2201/0662 ,  G02B21/16

Abstract: The present invention relates to a method for analyzing samples comprising spermatozoa, said method comprising the use of a flow-through counting compartment, wherein the time period between the end of loading and closing is carried out in a controlled and specified time period. The present invention further relates to a counting compartment or chamber suitable for said method and to a counting device comprising said counting compartment.

公开(公告)号：US11808758B2

公开(公告)日：2023-11-07

申请号：US17082483

申请日：2020-10-28

Applicant: S.D. SIGHT DIAGNOSTICS LTD.

Inventor： Joseph Joel Pollak ,  Sarah Levy Schreier ,  Yochay Shlomo Eshel ,  Amir Zait ,  Sharon Pecker ,  Trevor Ruggiero

IPC: G01N33/50 ,  G01N21/31 ,  G01N21/59 ,  G01N21/64 ,  G01N21/03

CPC classification number: G01N33/50 ,  G01N21/0303 ,  G01N21/31 ,  G01N21/59 ,  G01N21/64 ,  G01N2201/0662

Abstract: Apparatus and methods are described including placing a sample into a sample carrier that comprises a plurality of regions having upper and lower surfaces, having respective heights that are different from each other, and being configured such that cells form a monolayer, the monolayer within respective regions of the sample carrier having respective, different densities from each other, due to the respective regions of the sample carrier having respective heights that are different from each other. Microscopic images are acquired of each of the plurality of regions. Measurements are performed upon cell types that have a relatively high density upon microscopic images of a region of the sample chamber having a relatively low height, and measurements are performed upon cell types that have a relatively low density upon microscopic images of a region of the sample chamber having a relatively great height. Other applications are also described.

公开(公告)号：US20170003203A1

公开(公告)日：2017-01-05

申请号：US15112792

申请日：2015-06-30

Applicant: Dale Lee HEINTZELMAN, JR. ,  Beacon Technologies, LLC

Inventor： Dale Lee Heintzelman, JR.

IPC: G01N1/14 ,  B01L3/02 ,  G01N21/01 ,  G01N35/10

CPC classification number: B01L3/0275 ,  B01L3/021 ,  B01L9/543 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2300/0654 ,  B01L2300/0858 ,  B01L2300/123 ,  B01L2300/168 ,  G01N1/14 ,  G01N21/01 ,  G01N21/0303 ,  G01N21/13 ,  G01N21/59 ,  G01N35/10 ,  G01N2021/0367 ,  G01N2035/1062 ,  G01N2201/0662

Abstract: This disclosure is directed to exemplary embodiments of systems, methods, techniques, processes, products and product components that can facilitate users making improved absorbance or fluorescence measurements in the field of spectroscopy with reduced (minimal) sample waste, and increased throughput, particularly in the study of biological sciences. A measuring system is provided having: a base unit with a means for locating a pipette tip; a pipette tip designed to interact with the base unit for purposes of accurate pipette tip positioning; at least one light supplying unit positioned to supply light to a liquid sample in the pipette tip and at least one light collecting unit positioned to collect light from a liquid sample in the pipette tip.

Abstract translation: 本公开涉及系统，方法，技术，方法，产品和产品组件的示例性实施方案，其可以促进用户在减少（最小）样品废物和增加的产量的光谱学领域中进行改进的吸光度或荧光测量，特别是在 生物科学研究。 提供了一种测量系统，其具有：具有用于定位移液管尖端的装置的基座单元; 移液管尖端设计为与基座单元相互作用，用于精确移液管尖端定位; 至少一个供光单元，其被定位成向移液管尖端中的液体样品提供光，以及至少一个聚光单元，定位成收集来自移液管尖端中的液体样品的光。

公开(公告)号：US08947668B2

公开(公告)日：2015-02-03

申请号：US13617478

申请日：2012-09-14

Applicant: A Keith Hulme ,  John Hammond ,  Nathan Hulme

Inventor： A Keith Hulme ,  John Hammond ,  Nathan Hulme

IPC: G01N21/00 ,  G01N21/11 ,  G01N21/27

CPC classification number: G01N21/11 ,  G01N21/274 ,  G01N21/278 ,  G01N2201/0662 ,  G01N2201/1286

Abstract: A for traceably determining an unknown optical path length of a sample in an optical measuring device comprises the steps of: providing a drop analyzer connected to a standard spectrophotometer; providing a certified reference material contained in first and second closed high accuracy cuvettes; measuring absorbance of the certified reference material to obtain a first absorbance measurement for the first specified path length; measuring absorbance of the certified reference material for a second path length to obtain a second absorbance measurement; using a dropping device to drop a specified volume of the solvent on an optical surface so that the path length of the specified volume can be determined by reference to the first and second absorbance measurement; and using the dropping device to drop the same volume of sample as the specified volume of solvent on the optical measuring device.

Abstract translation: A，用于在光学测量装置中用于可追溯性地确定样品的未知光程长度包括以下步骤：提供连接到标准分光光度计的液滴分析仪; 提供包含在第一和第二封闭高精度比色杯中的认证参考材料; 测量认证参考材料的吸光度，以获得第一指定路径长度的第一吸光度测量; 测量第二路径长度的认证参考材料的吸光度以获得第二吸光度测量; 使用滴下装置将特定体积的溶剂落在光学表面上，使得可以参照第一和第二吸光度测量来确定指定体积的路径长度; 并使用滴液装置将相同体积的样品与特定体积的溶剂一起放在光学测量装置上。

公开(公告)号：US07796265B2

公开(公告)日：2010-09-14

申请号：US12109254

申请日：2008-04-24

Applicant: Michael Tkachuk

Inventor： Michael Tkachuk

IPC: G01N21/00

CPC classification number: G01N21/3504 ,  G01N2201/0662 ,  G01N2201/0668

Abstract: An optical absorption gas analyzer for determining the concentration of a target gas in a sample is disclosed. The analyzer comprises a chamber for containing the sample in use; a radiation source assembly arranged to emit radiation into the chamber; a first radiation detector assembly arranged to detect radiation transmitted along a first optical path through the chamber and a second radiation detector assembly arranged to detect radiation transmitted along a second optical path through the chamber, wherein the length of the second optical path which the sample can intercept is shorter than that of the first optical path. The analyzer further comprises a processor adapted to generate a sensing signal SS based on the detected radiation transmitted along the first optical path and a reference signal SR based on the detected radiation transmitted along the second optical path. The processor determines the concentration of the target gas in the sample based on a comparison of the sensing signal with the reference signal.

Abstract translation: 公开了一种用于测定样品中目标气体浓度的光吸收气体分析仪。 分析仪包括用于容纳使用中的样品的室; 辐射源组件，被布置成将辐射发射到所述腔室中; 第一辐射检测器组件，被布置成检测沿着通过所述室的第一光路传输的辐射;以及第二辐射检测器组件，其布置成检测沿着通过所述室的第二光路传输的辐射，其中所述样品可以 截距比第一光路的截距短。 分析器还包括处理器，其适于基于沿着第一光路传输的检测到的辐射和基于沿着第二光路传输的检测到的辐射的参考信号SR生成感测信号SS。 处理器基于感测信号与参考信号的比较来确定样品中目标气体的浓度。

公开(公告)号：US20090310138A1

公开(公告)日：2009-12-17

申请号：US12311042

申请日：2007-09-17

Applicant: Jarmo Vanhanen ,  Marcus Rinkiö ,  Päivi Törmä ,  Jouko Korppi-Tommola ,  Kari Loberg ,  Jukka Elfström

Inventor： Jarmo Vanhanen ,  Marcus Rinkiö ,  Päivi Törmä ,  Jouko Korppi-Tommola ,  Kari Loberg ,  Jukka Elfström

IPC: G01N21/00

CPC classification number: G01N21/8507 ,  G01N21/15 ,  G01N33/2888 ,  G01N2201/0662

Abstract: The invention relates to a method for monitoring the condition of a medium, based on the transmission/emission of light in a channel, in which a light is conducted through a medium layer defined by a measuring gap in a measuring head pushed in from an opening in the wall of the channel, the intensity of the light, or a variable proportional to it is measured through the medium layer, and the condition of the medium is evaluated, using measuring electronics, from the intensity of the change, according to set criteria. The measurement is performed using a sensor with a compact measuring head, in which the measuring electronics are essentially outside the channel, and in which the light is conducted to the measuring gap and away from the measuring gap by optical-fibre means. In addition, the invention also relates to a corresponding device.

Abstract translation: 本发明涉及一种基于通道中的光的透射/发射来监测介质的状态的方法，其中光通过由从开口推入的测量头中的测量间隙限定的介质层传导 在通道的壁上，通过介质层测量光的强度或与之成比例的变量，并且根据设定的标准，使用测量电子学根据变化的强度来评估介质的状况 。 使用具有紧凑测量头的传感器进行测量，其中测量电子器件基本上在通道外，并且光通过光纤装置传导到测量间隙并远离测量间隙。 此外，本发明还涉及相应的装置。

公开(公告)号：US20080123094A1

公开(公告)日：2008-05-29

申请号：US11792097

申请日：2005-12-01

Applicant: Henrik Vilstrup Juhl

Inventor： Henrik Vilstrup Juhl

IPC: G01J3/427

CPC classification number: G01N21/0303 ,  G01N21/31 ,  G01N2021/036 ,  G01N2201/066 ,  G01N2201/0662 ,  G01N2201/1293

Abstract: A spectrophotometer (2) comprising a source of radiation (6), preferably optical radiation, disposed to emit radiation at a plurality of wavelengths towards a sample in a sample holder (4) and a detection arrangement 8 for detecting the radiation after its interaction with the sample. The sample holder (4) is adapted to present a plurality of different path lengths for the emitted radiation through the sample. An arithmetic unit (10;10b) is operably connected to receive an intensity dependent output from the detection arrangement (8) and is adapted to store an intensity value of the detected emitted radiation indexed to its wavelength at two or more path lengths of the plurality of different path lengths and to calculate a value dependent on the ratio of the indexed intensity values at each of two path lengths by which an indication of the presence of a substance of interest withiA spectrophotometer (2) comprise a source of radiation (6), preferably optical radiation, disposed to emit radiation at a plurality of wavelengths towards a sample in a sample holder (4) and a detection arrangement 8 for detecting the radiation after its interaction with the sample. The sample holder (4) is adapted to present a plurality of different path lengths for the emitted radiation through the sample. An arithmetic unit (10;10b) is operably connected to receive an intensity dependent output from the detection arrangement (8) and is adapted to store an intensity value of the detected emitted radiation indexed to its wavelength at two or more path lengths of the plurality of different path lengths and to calculate a value dependent on the ratio of the indexed intensity values at each of two path lengths by which an indication of the presence of a substance of interest within the retained sample can be obtained.

Abstract translation: 一种分光光度计（2），包括辐射源（6），优选光辐射，被设置成朝向样品保持器（4）中的样品发射多个波长的辐射;以及检测装置8，用于在与 例子。 样品保持器（4）适于对通过样品的发射辐射呈现多个不同的路径长度。 算术单元（10; 10b）可操作地连接以接收来自检测装置（8）的强度相关输出，并且适于将检测到的发射的辐射的强度值存储在其波长的两个或更多个路径长度上 多个不同的路径长度并且计算取决于两个路径长度中的每一个处的索引强度值的比率的值，通过该值，用分光光度计（2）存在感兴趣的物质的指示包括辐射源（6） 优选地，光辐射被设置为发射多个波长的辐射朝向样本保持器（4）中的样本;以及检测装置8，用于在与样本相互作用之后检测辐射。 样品保持器（4）适于对通过样品的发射辐射呈现多个不同的路径长度。 算术单元（10; 10b）可操作地连接以接收来自检测装置（8）的强度相关输出，并且适于将检测到的发射的辐射的强度值存储在其波长的两个或更多个路径长度上 多个不同的路径长度，并且计算取决于两个路径长度中的每一个处的索引强度值的比率的值，由此可以获得在保留的样本内存在感兴趣的物质的指示。

公开(公告)号：US07248911B2

公开(公告)日：2007-07-24

申请号：US10802920

申请日：2004-03-18

Applicant: Kye-jin Jeon ,  Gil-won Yoon ,  In-duk Hwang ,  Sang-joon Han

Inventor： Kye-jin Jeon ,  Gil-won Yoon ,  In-duk Hwang ,  Sang-joon Han

IPC: A61B5/00

CPC classification number: A61B5/14532 ,  A61B5/0053 ,  A61B5/107 ,  A61B5/14546 ,  A61B5/1455 ,  A61B5/1495 ,  A61B2560/0223 ,  G01N21/359 ,  G01N2021/036 ,  G01N2021/3144 ,  G01N2201/0662

Abstract: In an apparatus and method of noninvasively measuring a concentration of a blood component, the method includes (a) varying a thickness of a body part of a subject, measuring absorption spectrums at different thicknesses of the body part, obtaining a first differential absorption spectrum between the absorption spectrums measured at different thicknesses, actually measuring concentrations of the blood component, and establishing a statistical model using the first differential absorption spectrum and the actually measured concentrations; and (b) estimating the concentration of the blood component using a second differential absorption spectrum obtained with respect to the body part based on the statistical model.

Abstract translation: 在非侵入性地测量血液成分浓度的装置和方法中，该方法包括：（a）改变被检体的身体部位的厚度，测量身体部位的不同厚度的吸收光谱，获得第一差异吸收光谱， 在不同厚度下测量的吸收光谱，实际测量血液成分的浓度，并使用第一个差示吸收光谱和实际测量的浓度建立统计模型; 和（b）基于统计模型使用相对于身体部位获得的第二差分吸收光谱估计血液成分的浓度。

公开(公告)号：US5482842A

公开(公告)日：1996-01-09

申请号：US224054

申请日：1994-04-07

Applicant: Klaus W. Berndt

Inventor： Klaus W. Berndt

IPC: C12M1/34 ,  C12Q1/04 ,  G01N21/27 ,  G01N21/35 ,  G01N21/59 ,  G06Q50/00

CPC classification number: G01N21/274 ,  C12M41/46 ,  G01N2021/5969 ,  G01N21/3504 ,  G01N2201/06106 ,  G01N2201/066 ,  G01N2201/0662 ,  Y10S435/808

Abstract: Methods and apparatus for detecting biological activity within a sample are disclosed. The present invention provides a combination of a first and a second infrared light source arranged on the side of a sample vial, and a first and a second narrow-band infrared detector similarly arranged on the side of the vial approximately opposite the sources. The disclosed arrangement cancels the sources of error while measuring the carbon dioxide content of the headspace gas above the sample. In operation, the present invention sequentially measures the photocurrents generated at each detector with no source turned on, with the first source turned on, and with the second source turned on and the first source turned off. The CO.sub.2 absorption coefficient of the vial headspace gas is then calculated based on the photocurrents measured. This present invention allows compensation for source aging, detector aging, and vial wall thickness changes. Moreover, the present invention permits a determination of the absolute absorption coefficient at a selected wavelength, most preferably about 4.26 .mu.m, which is the CO.sub.2 absorption characteristic wavelength. The determination of the absolute CO.sub.2 concentration within the headspace permits the detection of bacterial growth processes. Additionally, the disclosed source/detector combination can be produced at low cost. Thus, in preferred embodiments, the apparatus of the present invention comprises a plurality of vials that are simultaneously monitored by providing each of the plurality of vials with its own source/detector combination and activating and deactivating the sources and detectors using a multiplexer/demultiplexer arrangement.

Abstract translation: 公开了用于检测样品内生物活性的方法和装置。 本发明提供了布置在样品瓶侧面的第一和第二红外光源的组合，以及类似地布置在大致与源相对的小瓶侧面上的第一和第二窄带红外检测器。 所公开的布置在测量样品上方的顶空气体的二氧化碳含量的同时消除误差源。 在操作中，本发明顺序地测量在每个检测器处产生的光电流，其中第一源被接通，并且第二源被接通，并且第一源被关断。 然后基于测量的光电流计算出瓶顶空气体的CO 2吸收系数。 本发明允许补偿源老化，检测器老化和小瓶壁厚变化。 此外，本发明允许确定选定波长的绝对吸收系数，最优选约4.26μm，这是CO 2吸收特性波长。 顶部空间中绝对二氧化碳浓度的测定允许细菌生长过程的检测。 另外，所公开的源/检测器组合可以以低成本生产。 因此，在优选实施例中，本发明的装置包括多个小瓶，其通过向多个小瓶中的每一个提供自己的源/检测器组合来同时监视，并使用多路复用器/解复用器布置来激活和去激活源和检测器 。

公开(公告)号：US5371020A

公开(公告)日：1994-12-06

申请号：US050100

申请日：1993-05-07

Applicant: Peter A. Frischauf

Inventor： Peter A. Frischauf

IPC: G01N21/05 ,  G01N21/03 ,  G01N21/31 ,  G01N35/10

CPC classification number: G01N21/0303 ,  G01N2021/036 ,  G01N2021/0364 ,  G01N2021/3129 ,  G01N2035/1062 ,  G01N21/314 ,  G01N2201/0662

Abstract: A method of photometric in vitro determination of the content of an analyte in a sample is disclosed. The sample is located in a measuring chamber which has a radiation path length and has at least one at least partially transparent wall part. The measuring chamber is in optical communication with an optical system adapted for the analyte which includes a radiation source and a radiation detector. The measuring chamber is adjustable in shape which enables the radiation path length across the measuring chamber to be changed. In a first measuring step an unknown first radiation path length across the measuring chamber is set and radiation at at least two wavelengths is transmitted from the radiation source through the measuring chamber and to the radiation detector. In a second step, the measuring chamber is adjusted in shape thereby setting a second unknown path length across the measuring chamber. Radiation at the same wavelengths as during the first step is again transmitted from the radiation source through the measuring chamber and to the radiation detector. The analyte content is then determined on the basis of radiation detected in each of the measuring steps.

Abstract translation: PCT No.PCT / DK92 / 00280 Sec。 371日期：1993年5月7日 102（e）日期1993年5月7日PCT 1991年9月17日PCT公布。 出版物WO93 / 06456 日期：1993年4月1日。公开了一种光度测定样品中分析物含量的方法。 样品位于具有辐射路径长度并具有至少一个至少部分透明的壁部分的测量室中。 测量室与适用于包括辐射源和辐射探测器的分析物的光学系统光学通信。 测量室的形状可调节，使测量室上的辐射路径长度可以改变。 在第一测量步骤中，设置跨越测量室的未知的第一辐射路径长度，并且至少两个波长的辐射从辐射源通过测量室和辐射探测器传输。 在第二步骤中，测量室的形状被调节，从而在测量室上设置第二未知路径长度。 与第一步骤相同的波长的辐射再次从辐射源通过测量室和辐射探测器传输。 然后基于在每个测量步骤中检测到的辐射来确定分析物含​​量。