公开(公告)号：US5730985A

公开(公告)日：1998-03-24

申请号：US259004

申请日：1994-06-13

申请人： Brian H. Barber ,  Neal den Hollander ,  Jiri J. Krepinsky ,  M. Younus Meah

发明人： Brian H. Barber ,  Neal den Hollander ,  Jiri J. Krepinsky ,  M. Younus Meah

IPC分类号： A61K38/00 ,  A61P25/36 ,  C07F9/6561 ,  C07K16/44 ,  G01N33/94 ,  A61K39/385 ,  C07C69/76 ,  C07D451/12 ,  C07F9/02

CPC分类号： C07K16/44 ,  A61K39/0013 ,  C07F9/6561 ,  G01N33/946 ,  A61K2039/6081 ,  A61K2039/627 ,  A61K38/00

摘要： Methods are described for the rapid synthesis in satisfactory yield of methyl ecgonine phenylphosphonates as analogues of transition states for the hydrolysis of the benzoyl ester of an ecgonine derivative, namely cocaine, and their linking to carrier proteins, for the purpose of using them as immunogens. The resulting immunogens elicit the formation in experimental animals of antibodies able to promote the hydrolysis of cocaine. Both these catalytic anti-cocaine antibodies and the immunogens themselves are potentially useful for the treatment of individuals seeking to avoid the pharmacological effects of cocaine and in diagnostic applications.

公开(公告)号：US5501985A

公开(公告)日：1996-03-26

申请号：US230995

申请日：1994-04-21

申请人： Bennett W. Baugher ,  Aurora J. Chamberlain ,  Sharon M. Devereaux ,  Frank S. Ungemach

发明人： Bennett W. Baugher ,  Aurora J. Chamberlain ,  Sharon M. Devereaux ,  Frank S. Ungemach

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/94

CPC分类号： G01N33/948 ,  G01N33/54306 ,  G01N33/946 ,  G01N33/9486 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/816 ,  Y10S436/817 ,  Y10S436/822

摘要： Assay reagents, devices, methods and kits used in the analysis of low molecular weight analytes which by themselves are too small or unable to bind to two specific binding members at the same time. The invention involves the use of an analyte-substitute reagent (ASR) comprising at least two components, the first of which is identical to or an analog of the analyte to be determined, while the second is an unrelated ligand for which an antibody or other specific binding member can be obtained or produced.

摘要翻译： 用于分析低分子量分析物的试剂，装置，方法和试剂盒，它们本身太小或不能同时与两种特异性结合成员结合。 本发明涉及使用包含至少两种组分的分析物 - 替代试剂（ASR），其中第一组分与待测定的分析物相同或类似，而第二种是不相关的配体，抗体或其它组分 可以获得或制造特异性结合构件。

公开(公告)号：US5328828A

公开(公告)日：1994-07-12

申请号：US916568

申请日：1992-07-20

申请人： Mae W. Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07C233/18 ,  C07C323/12 ,  C07C323/29 ,  C07C323/32 ,  C07C323/41 ,  C07C323/60 ,  G01N33/535 ,  G01N33/577 ,  G01N33/94 ,  G01N33/536 ,  G01N33/537 ,  G01N33/542

CPC分类号： G01N33/946 ,  C07C233/18 ,  C07C323/12 ,  C07C323/32 ,  C07C323/41 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/822

摘要： The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

摘要翻译： 本发明涉及免疫测定，其可以通过使用至少两种共轭物来确定怀疑含有苯丙胺和/或甲基苯丙胺的样品中苯丙胺的存在，每个缀合物分别与分别与苯丙胺类似物和甲基苯丙胺类似物结合的功能相似的标记 以及针对苯丙胺的抗体和针对甲基苯丙胺的抗体，其中至少一种抗体是单克隆抗体。

公开(公告)号：US5326708A

公开(公告)日：1994-07-05

申请号：US115902

申请日：1993-09-01

申请人： Douglas E. Lewis

发明人： Douglas E. Lewis

IPC分类号： G01N33/94 ,  G01N33/00

CPC分类号： G01N33/946 ,  G01N33/948 ,  G01N33/9486 ,  Y10S436/901 ,  Y10T436/141111 ,  Y10T436/24 ,  Y10T436/25

摘要： Methods for collecting neonatal meconium samples, preparing meconium specimens for testing and for chemically analyzing neonatal meconium samples to determine their chemical composition are provided. A novel extraction method is employed in accordance with the invention to provide a non-aqueous, concentrated "cocktail" meconium extract containing substantially all of many possible target analytes in a single extraction step. Preliminary screening by fluorescence polarization immunoassay methods may be performed on the cocktail extract to qualitatively determine the presence of the target analytes in the meconium sample. If a positive preliminary result is obtained, new and improved quantitative GC/MS confirmatory procedures are provided by this invention to unequivocally identify and quantitate the amount of target analyte present in the sample in terms of nanograms of analyte per gram of meconium tested.

摘要翻译： 提供了收集新生儿胎粪样品，制备胎粪标本进行测试和化学分析新生儿胎粪样品以确定其化学成分的方法。 根据本发明采用新的提取方法，以在单次提取步骤中提供含有基本上所有许多可能的目标分析物的非水性浓缩“混合物”胎粪提取物。 通过荧光偏振免疫测定方法的初步筛选可以在混合物提取物上进行，以定性测定胎粪样品中目标分析物的存在。 如果获得了积极的初步结果，本发明提供了新的和改进的定量GC / MS确认程序，以每克测得的胎粪的纳克分析物来明确和定量存在于样品中的目标分析物的量。

公开(公告)号：US5262333A

公开(公告)日：1993-11-16

申请号：US898238

申请日：1992-06-12

申请人： Daniel F. Heiman ,  Sharon A. Johnson ,  Hsiang-Yun Y. Hu

发明人： Daniel F. Heiman ,  Sharon A. Johnson ,  Hsiang-Yun Y. Hu

IPC分类号： G01N33/542 ,  G01N33/94

CPC分类号： G01N33/946 ,  G01N33/542 ,  Y10S436/816

摘要： This disclosure relates to a method and reagents for determining amphetamine and d-methamphetamine in a biological fluid, such as urine. In particular, this disclosure relates to improvements in a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and d-methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross reactants such as .beta.-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4.0 to about 7.5 without adjustment to an alkaline pH, and contacting the sample with riboflavin binding protein to reduce interference from fluorescent components in the sample. The procedure also maintains the cross reactivity of the immunoassay for tyramine at about 0.4% and for 1-methamphetamine below about 5.1% and eliminates the necessity of using controlled substances as starting materials.

摘要翻译： 本公开涉及用于在生物流体例如尿中测定苯丙胺和d-甲基苯丙胺的方法和试剂。 特别地，本公开涉及在单次测定中用于确定苯丙胺和d-甲基苯丙胺的存在的荧光偏振免疫测定方法的改进，以及在这种方法中用作试剂的新型示踪剂化合物。 所述方法包括通过预先处理生物样品以消除交叉反应物例如β-羟基苯乙胺，通过仅用pH约4.0至约7.5的高碘酸钠溶液预温育样品，而不调节至碱性pH，并将样品与核黄素接触 结合蛋白以减少样品中荧光成分的干扰。 该方法还保持酪胺的免疫测定在约0.4％的交叉反应性和低于约5.1％的1-甲基苯丙胺，并且不需要使用受控物质作为起始原料。

公开(公告)号：US5145784A

公开(公告)日：1992-09-08

申请号：US816772

申请日：1992-01-02

申请人： Daniel E. Cox ,  Obsidiana Abril ,  Sara Bauminger ,  Bruce P. Neri ,  Lisa Shinefeld

发明人： Daniel E. Cox ,  Obsidiana Abril ,  Sara Bauminger ,  Bruce P. Neri ,  Lisa Shinefeld

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/94 ,  G01N35/00

CPC分类号： G01N33/54333 ,  G01N33/585 ,  G01N33/946 ,  G01N35/0098 ,  Y10S436/816 ,  Y10S436/901

摘要： A capillary flow device useful in double capture assays, such as double capture immunoassays, and a double capture assay. The capillary flow device comprises a capillary track, a sample receptacle, a particle collecting means and, optionally, a magnet and a particle concentrator. The assay method is useful for determining any analyte of interest for which there is a specific binding partner.

摘要翻译： 用于双重捕获测定的毛细管流动装置，例如双重捕获免疫测定和双重捕获测定。 毛细管流动装置包括毛细管轨道，样品容器，颗粒收集装置，以及可选地，磁体和颗粒集中器。 测定方法可用于确定任何具有特异性结合配偶体的感兴趣的分析物。

公开(公告)号：US5135863A

公开(公告)日：1992-08-04

申请号：US290487

申请日：1988-12-23

申请人： Mae Wan Leng Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

发明人： Mae Wan Leng Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07C233/18 ,  C07C323/12 ,  C07C323/29 ,  C07C323/32 ,  C07C323/41 ,  C07C323/60 ,  G01N33/535 ,  G01N33/577 ,  G01N33/94

CPC分类号： G01N33/946 ,  C07C233/18 ,  C07C323/12 ,  C07C323/32 ,  C07C323/41 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/822

摘要： The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

公开(公告)号：US4329281A

公开(公告)日：1982-05-11

申请号：US106347

申请日：1979-12-21

申请人： James G. Christenson ,  Benjamin Pecherer

发明人： James G. Christenson ,  Benjamin Pecherer

IPC分类号： C07D207/46 ,  G01N33/94 ,  A61K39/395 ,  A61K39/385 ,  C07G7/00 ,  G01N33/54

CPC分类号： C07D207/46 ,  G01N33/946 ,  Y10S436/822 ,  Y10S530/806

摘要： Novel hapten compositions consisting of 4-[4-[2-(aminopropyl)]phenyl]butanoic acid and its N-methyl derivative are useful in preparing immunogens which can be respectively employed in the elicitation of antibodies selective to amphetamine and methamphetamine. These antibodies can be used as reagents in immunoassays for these two compounds.

摘要翻译： 由4- [4- [2-（氨基丙基）]苯基]丁酸及其N-甲基衍生物组成的新型半抗原组合物可用于制备可分别用于引发对苯丙胺和甲基苯丙胺敏感的抗体的免疫原。 这些抗体可用作这两种化合物的免疫测定中的试剂。

公开(公告)号：US4191613A

公开(公告)日：1980-03-04

申请号：US760499

申请日：1977-01-19

申请人： Edwin F. Ullman ,  Kenneth E. Rubenstein

发明人： Edwin F. Ullman ,  Kenneth E. Rubenstein

IPC分类号： C07J19/00 ,  C07J41/00 ,  C07J43/00 ,  G01N33/535 ,  G01N33/78 ,  G01N33/94 ,  G01N31/14 ,  C07G7/02

CPC分类号： C07J41/0016 ,  C07J19/00 ,  C07J43/003 ,  G01N33/535 ,  G01N33/78 ,  G01N33/946 ,  G01N33/948 ,  G01N33/9486 ,  Y10S435/964

摘要： Novel conjugated enzyme compositions are provided for use in homogeneous enzyme immunoassays. A wide variety of compounds, particularly drugs, including drugs of abuse, drugs used in repetitive therapeutic applications, hormones, and the like, are conjugated to malate dehydrogenase. The resulting products have a high turnover rate, so as to provide a high multiplication factor when employed in a homogeneous immunoassay, have long storage lives, and allow for the accurate and sensitive detection of compounds of interest.

摘要翻译： 提供新型共轭酶组合物用于均相酶免疫测定。 与各种各样的化合物，特别是药物，包括滥用药物，用于重复治疗应用的药物，激素等都与苹果酸脱氢酶缀合。 所得产品具有高的更换率，以便在均相免疫测定中使用时提供高倍增因子，具有长存储寿命，并且允许精确和灵敏地检测目的化合物。

公开(公告)号：US4190496A

公开(公告)日：1980-02-26

申请号：US802683

申请日：1977-06-02

申请人： Kenneth E. Rubenstein ,  Edwin F. Ullman

发明人： Kenneth E. Rubenstein ,  Edwin F. Ullman

IPC分类号： G01N33/542 ,  G01N33/94 ,  G01N31/14

CPC分类号： G01N33/948 ,  G01N33/542 ,  G01N33/946 ,  G01N33/9486 ,  Y10S435/966 ,  Y10S930/26

摘要： Novel biological assay method for determining the presence of a specific organic material by employing a modified enzyme for amplification. By employing receptors specific for one or a group of materials (hereinafter referred to as "ligands") and binding an enzyme to the ligand or ligand counterfeit to provide an "enzyme-bound-ligand", an extremely sensitive method is provided for assaying for ligands. The receptor when bound to the enzyme-bound-ligand substantially inhibits enzymatic activity, providing for different catalytic efficiencies of enzyme-bound-ligand and enzyme-bound-ligand combined with receptor.The receptor, ligand and enzyme-bound-ligand are combined in an arbitrary order and the effect of the presence of ligand on enzymatic activity determined. Various protocols may be used for assaying for enzymatic activity and relating the result to the amount of ligand present.The subject method may also be used for determining receptors, employing the same procedure, except for not including receptor as a reagent.

摘要翻译： 用于通过使用经修饰的酶进行扩增来确定特定有机材料的存在的新型生物测定方法。 通过使用对一种或一组材料（以下称为“配体”）特异性的受体并将酶与配体或配体假冒结合以提供“酶结合 - 配体”，提供极其灵敏的方法用于测定 配体。 当与酶结合的配体结合时的受体基本上抑制酶活性，提供与受体结合的酶结合配体和酶结合配体的不同催化效率。 受体，配体和酶结合配体以任意顺序组合，并确定配体存在对酶活性的影响。 可以使用各种方案来测定酶活性并将结果与​​存在的配体的量相关联。 本方法也可以用于确定受体，采用相同的方法，但不包括受体作为试剂。