公开(公告)号：US20100173429A1

公开(公告)日：2010-07-08

申请号：US12727612

申请日：2010-03-19

申请人： Hashem AKHAVAN-TAFTI ,  Renuka De SILVA ,  Wenhua XIE

发明人： Hashem AKHAVAN-TAFTI ,  Renuka De SILVA ,  Wenhua XIE

IPC分类号： G01N33/566 ,  G01N21/76 ,  G01N33/533

CPC分类号： G01N33/582 ,  C07D219/02 ,  C07D409/04 ,  G01N33/53 ,  Y10S435/968 ,  Y10T436/13 ,  Y10T436/143333 ,  Y10T436/145555

摘要： Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.

摘要翻译： 提供了使用化学发光标记化合物和化学发光标记的缀合物的方法。 该化合物包含带有环外烯酮二硫缩醛基团的吖啶环，并且还含有允许附着于感兴趣的化合物的标记取代基。 新型化学发光化合物和标记的共轭物方便制备，高度稳定，并根据需要快速生成化学发光。 化合物和缀合物可用于样品中分析物的测定和使用标记的特异性结合对的测定。

公开(公告)号：US07682839B2

公开(公告)日：2010-03-23

申请号：US11724727

申请日：2007-03-16

申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua Xie

发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua Xie

IPC分类号： G01N33/533 ,  G01N21/76 ,  C07K1/10 ,  C12Q1/28

CPC分类号： G01N33/582 ,  C07D219/02 ,  C07D409/04 ,  G01N33/53 ,  Y10S435/968 ,  Y10T436/13 ,  Y10T436/143333 ,  Y10T436/145555

摘要： Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.

摘要翻译： 提供了使用化学发光标记化合物和化学发光标记的缀合物的方法。 该化合物包含带有环外烯酮二硫缩醛基团的吖啶环，并且还含有允许附着于感兴趣的化合物的标记取代基。 新型化学发光化合物和标记的共轭物方便制备，高度稳定，并根据需要快速生成化学发光。 化合物和缀合物可用于样品中分析物的测定和使用标记的特异性结合对的测定。

公开(公告)号：US20090246819A1

公开(公告)日：2009-10-01

申请号：US12476668

申请日：2009-06-02

申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua XIE

发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua XIE

IPC分类号： C12Q1/28

CPC分类号： C07D219/02 ,  C07D219/04 ,  C07D219/06 ,  C07D409/04 ,  C07D409/06 ,  C07D411/04 ,  C07D411/06 ,  C07F9/64 ,  C07F9/65586 ,  C12Q1/28 ,  G01N33/581 ,  G01N2333/908

摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.

摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。

公开(公告)号：US20070264664A1

公开(公告)日：2007-11-15

申请号：US11799895

申请日：2007-05-03

申请人： Hashem Akhavan-Tafti

发明人： Hashem Akhavan-Tafti

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/54306

摘要： Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays.

公开(公告)号：US06774249B2

公开(公告)日：2004-08-10

申请号：US09965046

申请日：2001-09-27

申请人： Hashem Akhavan-Tafti ,  Richard S. Handley ,  Mark D. Sandison ,  Randall K. Larkin

发明人： Hashem Akhavan-Tafti ,  Richard S. Handley ,  Mark D. Sandison ,  Randall K. Larkin

IPC分类号： C01B1332

CPC分类号： C01B13/02 ,  C08F8/18 ,  C08F8/40 ,  C08F8/26 ,  C08F212/08 ,  C08F212/14 ,  C08F212/36

摘要： Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.

摘要翻译： 公开了改进的聚合物固定化光敏剂以及制备和使用它们的方法。 聚合物固定的光敏剂包括交联的聚合物主链，共价结合到聚合物主链上的多个阳离子铵或鏻基团和固定的光敏剂。 铵或鏻基团中的平均总碳原子数为至少四个，优选至少12.光敏剂可以共价或离子地结合到聚合物上。 本发明的聚合物负载的光敏剂在催化含有碳 - 碳双键的化合物的光敏化氧化方面出人意料地优异。

公开(公告)号：US06635437B2

公开(公告)日：2003-10-21

申请号：US10054417

申请日：2002-01-22

申请人： Hashem Akhavan-Tafti ,  Zahra Arghavani ,  Renuka DeSilva

发明人： Hashem Akhavan-Tafti ,  Zahra Arghavani ,  Renuka DeSilva

IPC分类号： C12Q142

CPC分类号： C07F9/6541 ,  C07F9/5765 ,  C07F9/64 ,  C12Q1/42 ,  C12Q2334/00 ,  Y10S435/968

摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon—carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group. Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.

摘要翻译： 提供了与磷酸酶反应产生化学发光的新型杂环化合物以及其制备方法和可用于其中的中间体。 该化合物包含带有环外碳 - 碳双键的氮，氧或含硫杂环体系。 该双键在远端碳被磷酸基团和含氧或含硫原子的基团进一步取代。 提供除了杂环磷酸酯化合物之外还包含阳离子芳族化合物（CAC）的新型组合物。 在组合物中添加CAC大大增加了化学发光的产生并提供了改善的检测灵敏度。 进一步包含阴离子表面活性剂和非离子表面活性剂的组合物提供检测灵敏度的另外的改进。 新颖的化学发光化合物和组合物可用于生产光的方法和用于磷酸酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。

公开(公告)号：US06270695B1

公开(公告)日：2001-08-07

申请号：US09358003

申请日：1999-07-21

申请人： Hashem Akhavan-Tafti ,  Zahra Arghavani ,  Renuka DeSilva

发明人： Hashem Akhavan-Tafti ,  Zahra Arghavani ,  Renuka DeSilva

IPC分类号： C09K300

CPC分类号： C07F9/6541 ,  C07F9/5765 ,  C07F9/64 ,  C12Q1/42 ,  C12Q2334/00 ,  Y10S435/968

摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group. Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.

摘要翻译： 提供了与磷酸酶反应产生化学发光的新型杂环化合物以及其制备方法和可用于其中的中间体。 该化合物包含带有环外碳 - 碳双键的氮，氧或含硫杂环体系。 提供了双键在磷酸基和含氧或含硫原子的基团的末端碳上进一步取代的。还提供除了杂环磷酸酯化合物之外还包含阳离子芳族化合物（CAC）的新型组合物。 在组合物中添加CAC大大增加了化学发光的产生并提供了改善的检测灵敏度。 进一步包含阴离子表面活性剂和非离子表面活性剂的组合物提供检测灵敏度的另外的改进。 新颖的化学发光化合物和组合物可用于生产光的方法和用于磷酸酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。

公开(公告)号：US06245928B1

公开(公告)日：2001-06-12

申请号：US09506263

申请日：2000-02-17

申请人： Zahra Arghavani ,  Hashem Akhavan-Tafti ,  Renuka DeSilva ,  Kumar Thakur

发明人： Zahra Arghavani ,  Hashem Akhavan-Tafti ,  Renuka DeSilva ,  Kumar Thakur

IPC分类号： C07F906

CPC分类号： G01N33/582 ,  C07C69/734 ,  C07C2603/74 ,  C07D321/00 ,  C07F9/12 ,  C07F9/65512 ,  G01N33/5308 ,  G01N33/54326

摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.

摘要翻译： 提供了具有改善的水溶性和储存稳定性的稳定的，酶促触发的化学发光1,2-二氧杂环丁烷，以及用于其制备的合成方法和中间体。 二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的测定中时，通过消除试剂残留的问题而提供优异的性能。 这些二氧杂环丁烷在储存时显示出显着提高的稳定性。 还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。

公开(公告)号：US06218137B1

公开(公告)日：2001-04-17

申请号：US09540796

申请日：2000-03-31

申请人： Hashem Akhavan-Tafti ,  Zahra Arghavani ,  Renuka DeSilva

发明人： Hashem Akhavan-Tafti ,  Zahra Arghavani ,  Renuka DeSilva

IPC分类号： C12Q142

CPC分类号： C07F9/6541 ,  C07F9/5765 ,  C07F9/64 ,  C12Q1/42 ,  C12Q2334/00 ,  Y10S435/968

摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group. Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.

摘要翻译： 提供了与磷酸酶反应产生化学发光的新型杂环化合物以及其制备方法和可用于其中的中间体。 该化合物包含带有环外碳 - 碳双键的氮，氧或含硫杂环体系。 提供了双键在磷酸基和含氧或含硫原子的基团的末端碳上进一步取代的。还提供除了杂环磷酸酯化合物之外还包含阳离子芳族化合物（CAC）的新型组合物。 在组合物中添加CAC大大增加了化学发光的产生并提供了改善的检测灵敏度。 进一步包含阴离子表面活性剂和非离子表面活性剂的组合物提供检测灵敏度的另外的改进。 新颖的化学发光化合物和组合物可用于生产光的方法和用于磷酸酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。

公开(公告)号：US6126870A

公开(公告)日：2000-10-03

申请号：US99657

申请日：1998-06-17

申请人： Hashem Akhavan-Tafti

发明人： Hashem Akhavan-Tafti

IPC分类号： C07D219/00 ,  C07D219/02 ,  C07D219/04 ,  C07D221/08 ,  C07F7/18 ,  C07F9/113 ,  C07F9/64 ,  C07F9/6553 ,  C09K11/06 ,  C09K11/07 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/533 ,  G01N33/58 ,  C09K3/00

CPC分类号： C07D221/08 ,  C07D219/02 ,  C07F7/1856 ,  C07F9/113 ,  C07F9/64 ,  C07F9/655372 ,  C09K11/06 ,  G01N33/533 ,  G01N33/582

摘要： Chemiluminescent labeling compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic double bond and further contain a labeling substituent which permits attachement to compounds of interest. The novel chemiluminescent compounds and labeled conjugates generated chemiluminescence rapidly after undergoing a reaction with an acid, an oxidant and a base. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.

摘要翻译： 提供化学发光标记化合物和化学发光标记的缀合物。 所述化合物包含带有环外双键的吖啶环，并且还含有允许附着于感兴趣的化合物的标记取代基。 新型化学发光化合物和标记的共轭物在与酸，氧化剂和碱反应后迅速产生化学发光。 化合物和缀合物可用于样品中分析物的测定和使用标记的特异性结合对的测定中。