利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

116 条记录 (耗时 0.065 秒)

    Vaccines against multiple subtypes of influenza virus
    42.
    发明授权
    Vaccines against multiple subtypes of influenza virus 有权
    针对流感病毒多种亚型的疫苗

    公开(公告)号：US09592285B2

    公开(公告)日：2017-03-14

    申请号：US12269824

    申请日：2008-11-12

    申请人： Ruxandra Draghia-Akli ,  David B. Weiner ,  Jian Yan ,  Dominick Laddy

    发明人： Ruxandra Draghia-Akli ,  David B. Weiner ,  Jian Yan ,  Dominick Laddy

    IPC分类号： A61K39/12 ,  A61K9/00 ,  A61K38/20 ,  C07K14/005 ,  A61K47/34 ,  A61K39/145 ,  A61K39/00

    CPC分类号： A61K39/145 ,  A61K9/0019 ,  A61K38/208 ,  A61K38/2086 ,  A61K39/12 ,  A61K47/34 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/58 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16034 ,  C12N2760/16122 ,  C12N2760/16134 ,  A61K2300/00

    摘要： An aspect of the present invention is directed towards DNA plasmid vaccines capable of generating in a mammal an immune response against a plurality of influenza virus subtypes, comprising a DNA plasmid and a pharmaceutically acceptable excipient. The DNA plasmid is capable of expressing a consensus influenza antigen in a cell of the mammal in a quantity effective to elicit an immune response in the mammal, wherein the consensus influenza antigen comprises consensus hemagglutinin (HA), neuraminidase (NA), matrix protein, nucleoprotein, M2 ectodomain-nucleo-protein (M2e-NP), or a combination thereof. Preferably the consensus influenza antigen comprises HA, NA, M2e-NP, or a combination thereof. The DNA plasmid comprises a promoter operably linked to a coding sequence that encodes the consensus influenza antigen. Additionally, an aspect of the present invention includes methods of eliciting an immune response against a plurality of influenza virus subtypes in a mammal using the DNA plasmid vaccines provided.

    摘要翻译： 本发明的一个方面涉及能够在哺乳动物中产生针对多种流感病毒亚型的免疫应答的DNA质粒疫苗，其包含DNA质粒和药学上可接受的赋形剂。 DNA质粒能够以有效引发哺乳动物免疫应答的量在哺乳动物细胞中表达共有流感抗原，其中共有流感抗原包含共有血凝素（HA），神经氨酸酶（NA），基质蛋白， 核蛋白，M2胞外结构域 - 核蛋白（M2e-NP）或其组合。 优选地，共有流感抗原包含HA，NA，M2e-NP或其组合。 DNA质粒包含与编码共有流感抗原的编码序列可操作地连接的启动子。 此外，本发明的一个方面包括使用提供的DNA质粒疫苗在哺乳动物中引发针对多种流感病毒亚型的免疫应答的方法。

    Vaccines and immunotherapeutics using codon optimized IL-15 and methods for using the same
    46.
    发明授权
    Vaccines and immunotherapeutics using codon optimized IL-15 and methods for using the same 有权
    使用密码子优化的IL-15的疫苗和免疫治疗剂及其使用方法

    公开(公告)号：US08178660B2

    公开(公告)日：2012-05-15

    申请号：US12160766

    申请日：2007-01-12

    申请人： David B. Weiner ,  Michele Kutzler

    发明人： David B. Weiner ,  Michele Kutzler

    IPC分类号： C07H21/02 ,  C07H21/04 ,  A61K48/00

    CPC分类号： C07K14/5443 ,  A61K2039/55522 ,  Y02A50/394

    摘要： Nucleic acid molecules that encode IL-15 or fragments thereof, which express protein at a higher level than nucleic acid molecules with native coding sequences for IL-15 are disclosed. Nucleic acid molecules with additional modifications such as the absence of coding sequences for IL-15 signal sequences and/or the absence of IL-15 untranslated sequences and/or inclusion of non-IL-15 signal sequences are also disclosed. Vectors, including plasmids and viral vectors, comprising such nucleic acid molecules; and to host cells comprising such nucleic acid molecules are disclosed as well as methods of using such nucleic acid molecules alone or in combination with nucleic acid sequences encoding immunogens which are part of the nucleic acid molecules and/or part of a different nucleic acid molecule. Recombinant vaccines and live attenuated pathogens encoding fusion proteins, and methods of using the same, are disclosed.

    摘要翻译： 公开了编码IL-15或其片段的核酸分子，其以比具有IL-15的天然编码序列的核酸分子更高的水平表达蛋白质。 还公开了具有附加修饰的核酸分子，例如不存在IL-15信号序列的编码序列和/或不存在IL-15非翻译序列和/或包含非IL-15信号序列。 包括这种核酸分子的载体，包括质粒和病毒载体; 以及包含这种核酸分子的宿主细胞以及使用这些核酸分子单独或与编码作为核酸分子和/或不同核酸分子的一部分的免疫原的核酸序列组合的方法。 公开了重组疫苗和编码融合蛋白的活减毒病原体及其使用方法。

    Nucleic acid sequences encoding and compositions comprising IgE signal peptide and/or IL-15 and methods for using the same
    47.
    发明授权
    Nucleic acid sequences encoding and compositions comprising IgE signal peptide and/or IL-15 and methods for using the same 有权
    编码核酸序列和包含IgE信号肽和/或IL-15的组合物及其使用方法

    公开(公告)号：US08173786B2

    公开(公告)日：2012-05-08

    申请号：US10560650

    申请日：2004-06-14

    申请人： David B. Weiner ,  Michele Kutzler ,  Andrew Y. Choo ,  Joo-Sung Yang ,  Jean D. Boyer

    发明人： David B. Weiner ,  Michele Kutzler ,  Andrew Y. Choo ,  Joo-Sung Yang ,  Jean D. Boyer

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C07K14/5443 ,  A61K2039/53 ,  A61K2039/55527 ,  C07K14/70578 ,  C07K2319/02 ,  Y02A50/394

    摘要： Fusion proteins and nucleic acid molecules encoding fusion proteins are disclosed. Fusion proteins comprising non-IL-15 signal peptide linked to IL-15 protein sequences and fusion proteins comprising an IgE signal peptide linked to non-IgE protein sequences are disclosed. Vectors comprising such nucleic acid molecules; and to host cells comprising such vectors are disclosed as well as recombinant vaccines and live attenuated pathogens encoding fusion proteins, and methods of using the same, are disclosed. The immunomodulatory effect following delivery of IL-15 and CD40L, with or without immunogens, is disclosed as are various nucleic acid molecules and compositions thereof used for delivering such proteins and methods of using such compositions.

    摘要翻译： 公开了融合蛋白和编码融合蛋白的核酸分子。 公开了包含与IL-15蛋白质序列连接的非IL-15信号肽和包含与非IgE蛋白序列连接的IgE信号肽的融合蛋白的融合蛋白。 包含这种核酸分子的载体; 公开了包含这种载体的宿主细胞以及编码融合蛋白的重组疫苗和活减毒病原体及其使用方法。 公开了递送IL-15和CD40L（含有或不含免疫原）后的免疫调节作用，用于递送此类蛋白质的各种核酸分子及其组合物以及使用该组合物的方法。

    Compositions for and methods of using herpes simplex virus glycoprotein D to suppress immune responses
    48.
    发明授权
    Compositions for and methods of using herpes simplex virus glycoprotein D to suppress immune responses 有权
    使用单纯疱疹病毒糖蛋白D的组合物和方法来抑制免疫应答

    公开(公告)号：US07569218B2

    公开(公告)日：2009-08-04

    申请号：US10333802

    申请日：2001-07-27

    申请人： David B. Weiner ,  Jeong-Im Sin

    发明人： David B. Weiner ,  Jeong-Im Sin

    IPC分类号： A61K48/00

    CPC分类号： A61K48/00 ,  A61K38/162 ,  A61K39/385 ,  A61K2039/53 ,  A61K2039/6075 ,  C07K14/005 ,  C12N2710/10343 ,  C12N2710/16622

    摘要： Methods of delivering a desired polypeptide to an individual are disclosed. The methods comprise administering to the individual an immunogenic vector comprising a nucleic acid encoding the desired polypeptide operably linked to regulatory elements in combination with one or more of gD protein, a function fragment of gD protein, a nucleic acid encoding gD protein operably linked to regulatory elements, or a nucleic acid encoding a functional fragment of gD protein operably linked to regulatory elements. Compositions comprising an immunogenic vector that comprises a nucleic acid encoding the desired polypeptide operably linked to regulatory elements; and one or more of gD protein, a function fragment of gD protein, a nucleic acid encoding gD protein operably linked to regulatory elements, or a nucleic acid encoding a functional fragment of gD protein operably linked to regulatory elements are disclosed. Methods for inhibiting an undesirable immune response in an individual are disclosed.

    摘要翻译： 公开了将期望的多肽递送给个体的方法。 所述方法包括向个体施用免疫原性载体，其包含编码所需多肽的核酸，其与调节元件结合使用，所述多核苷酸可与gD蛋白，gD蛋白的功能片段，编码可调控的 元件或编码与调节元件可操作地连接的gD蛋白的功能性片段的核酸。 包含免疫原性载体的组合物，其包含编码可操作地连接到调节元件的所需多肽的核酸; 并且公开了一种或多种gD蛋白，gD蛋白的功能片段，编码与调控元件可操作地连接的gD蛋白的核酸，或编码与调节元件可操作地连接的gD蛋白的功能性片段的核酸。 公开了抑制个体中不期望的免疫应答的方法。

    Cosmid dna constructs and methods of making and using same
    49.
    发明申请
    Cosmid dna constructs and methods of making and using same 有权
    粘粒dna构建及其制作和使用方法

    公开(公告)号：US20080219953A1

    公开(公告)日：2008-09-11

    申请号：US10168694

    申请日：2000-12-22

    申请人： David B. Weiner ,  Jeong-Im Sin ,  Donghui Zhang

    发明人： David B. Weiner ,  Jeong-Im Sin ,  Donghui Zhang

    IPC分类号： A61K48/00 ,  A61K31/445 ,  C12N15/86

    CPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/54 ,  C12N2710/16634

    摘要： Methods of inducing immune responses in individuals against a pathogen are disclosed. Methods of treating individuals susceptible to or suffering from a disease associated with a genetic defect which results in the non-production or under production of a protein or the production of a non-functioning or partially functioning protein are disclosed. Methods of delivering a protein to an individual are disclosed. Pharmaceutical compositions that comprise cosmids are disclosed.

    摘要翻译： 公开了在针对病原体的个体中诱导免疫应答的方法。 公开了治疗导致非生产或正在产生蛋白质或产生不起作用或部分功能的蛋白质的遗传缺陷相关疾病或患有疾病的个体的方法。 公开了向个体递送蛋白质的方法。 公开了包含粘粒的药物组合物。