公开(公告)号：US5981505A

公开(公告)日：1999-11-09

申请号：US979385

申请日：1997-11-26

申请人： David B. Weiner ,  William V. Williams ,  Bin Wang

发明人： David B. Weiner ,  William V. Williams ,  Bin Wang

IPC分类号： A61K39/00 ,  A61K39/21 ,  C07K14/16 ,  C12N15/87 ,  A61K45/05 ,  A61K48/00 ,  A61K31/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  C12N15/87 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K39/0011 ,  C12N2740/16034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234 ,  C12N2740/16322 ,  Y10S514/818

摘要： Methods of inducing genetic material into cells of an individual and compositions and kits for practicing the same are disclosed. The methods comprise the steps of contacting cells of an individual with a polynucleotide function enhancer and administering to the cells, a nucleic acid molecule that is free of retroviral particles. The nucleic acid molecule comprises a nucleotide sequence that encodes a protein that comprises at least one epitope that is identical or substantially similar to an epitope of a pathogen antigen or an antigen associated with a hyperproliferative or autoimmune disease, a protein otherwise missing from the individual due to a missing, non-functional or partially functioning gene, or a protein that produces a therapeutic effect on an individual. Methods of prophylactically and therapeutically immunizing an individual against HIV are disclosed. Pharmaceutical compositions and kits for practicing methods of the present invention are disclosed.

摘要翻译： PCT No.PCT / US94 / 00899 Sec。 371日期：1995年8月28日 102（e）日期1995年8月28日PCT 1994年1月26日PCT PCT。 公开号WO94 / 16737 日期1994年8月4日公开了将遗传物质诱导入个体细胞的方法和用于实施其的组合物和试剂盒。 所述方法包括使个体的细胞与多核苷酸功能增强子接触并向细胞施用不含逆转录病毒颗粒的核酸分子的步骤。 核酸分子包含编码蛋白质的核苷酸序列，该蛋白质包含与病原体抗原或与过度增殖或自身免疫疾病相关的抗原的表位相同或基本相似的至少一个表位， 涉及缺失的，非功能的或部分功能的基因或对个体产生治疗作用的蛋白质。 公开了预防和治疗个体免受HIV感染的方法。 公开了用于本发明的实践方法的药物组合物和试剂盒。

公开(公告)号：US5840300A

公开(公告)日：1998-11-24

申请号：US526583

申请日：1995-09-11

申请人： William V. Williams ,  David B. Weiner

发明人： William V. Williams ,  David B. Weiner

IPC分类号： A61K38/00 ,  C07K16/00 ,  C07K16/10 ,  A61K39/395 ,  A61K39/42 ,  C07H21/02

CPC分类号： C07K16/00 ,  C07K16/1063 ,  A61K38/00

摘要： Methods and compositions for the generation of single chain antibody fragments.

摘要翻译： 用于产生单链抗体片段的方法和组合物。

公开(公告)号：US11419925B2

公开(公告)日：2022-08-23

申请号：US14775047

申请日：2014-03-14

申请人： David B. Weiner ,  Jian Yan ,  Karuppiah Muthumani ,  Jewell Walters

发明人： David B. Weiner ,  Jian Yan ,  Karuppiah Muthumani ,  Jewell Walters

IPC分类号： A61K39/00 ,  A61K39/395 ,  C12N9/02

摘要： Disclosed herein are compositions and methods for treating cancer and in particular vaccines that treat and provide protection against tumor growth.

公开(公告)号：US10398769B2

公开(公告)日：2019-09-03

申请号：US14910136

申请日：2014-08-04

申请人： The Trustees of the University of Pennsylvania ,  Inovio Pharmaceuticals, Inc. ,  David B. Weiner ,  Jian Yan

发明人： David B. Weiner ,  Jian Yan

IPC分类号： A61K39/145 ,  A61K39/12 ,  C12N7/00 ,  C07K14/005 ,  A61K39/00

摘要： Provided herein are nucleic acid sequences that encode novel consensus amino acid sequences of HA Influenza A of serotype H7N9 alone and in combination with HA hemagglutinin and/or influenza B hemagglutinin, as well as genetic constructs/vectors and vaccines expressing the sequences. Also provided herein are methods for generating an immune response against one or more influenza A serotypes and/or influenza B serotypes, or combinations thereof, using the vaccines that are provided.

公开(公告)号：US09597388B2

公开(公告)日：2017-03-21

申请号：US14391952

申请日：2013-04-12

申请人： David B. Weiner ,  Devon Shedlock

发明人： David B. Weiner ,  Devon Shedlock

IPC分类号： A61K39/12 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K38/20 ,  A61K38/208 ,  A61K38/2086 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/57 ,  A61K2039/572 ,  A61K2039/70 ,  A61N1/327 ,  C07K14/005 ,  C07K2319/02 ,  C12N7/00 ,  C12N2760/14122 ,  C12N2760/14134 ,  C12N2760/14171 ,  C12N2760/14222 ,  C12N2760/14234 ,  C12N2760/14271

摘要： Nucleic acid molecules and compositions comprising one or more nucleic acid sequences that encode a consensus filovirus immunogen including a consensus Marburgvirus filovirus glycoprotein MARV GP immunogen, a consensus Ebolavirus Sudan filovirus glycoprotein SEBOV GP immunogen and a consensus Ebolavirus Zaire glycoprotein ZEBOV GP immunogen are disclosed. The coding sequences optionally include operable linked coding sequence that encode a signal peptide. Immunomodulatory methods and methods of inducing an immune response against filovirus, particularly Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Method of preventing filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire and methods of treating individuals infected with filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Consensus filovirus proteins are disclosed.

公开(公告)号：US20160022796A1

公开(公告)日：2016-01-28

申请号：US14774399

申请日：2014-03-17

申请人： David B. WEINER ,  Daniel VILLARREAL ,  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： David B. Weiner ,  Daniel Villarreal

IPC分类号： A61K39/04 ,  A61K45/06

CPC分类号： A61K39/04 ,  A61K39/07 ,  A61K45/06 ,  A61K2039/53

摘要： Compositions comprising a nucleic acid molecule that encodes TB esat-6 proteins are disclosed. Methods of inducing an immune response against TB an individual are disclosed. Method of treating an individual who has been diagnosed with TB are disclosed. Method of preventing TB infection in an individual are disclosed.

摘要翻译： 公开了包含编码TB esat-6蛋白的核酸分子的组合物。 公开了诱导个体对TB的免疫应答的方法。 公开了治疗已被诊断患有结核病的个体的方法。 公开了预防结核病感染个体的方法。

公开(公告)号：US08852609B2

公开(公告)日：2014-10-07

申请号：US12936186

申请日：2009-04-06

申请人： David B. Weiner ,  Karuppiah Muthumani

发明人： David B. Weiner ,  Karuppiah Muthumani

IPC分类号： A61K39/12 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00 ,  A61K39/39

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/39 ,  A61K2039/53 ,  A61K2039/55516 ,  A61K2039/55522 ,  A61K2039/55588 ,  A61K2039/575 ,  C07H21/04 ,  C12N7/00 ,  C12N2770/36121 ,  C12N2770/36122 ,  Y02A50/383

摘要： Consensus CHIKV E1 protein, consensus CHIKV E2 protein, consensus CHIKV capsid protein, or fragments and homologues thereof, and nucleic acid molecules that encode the same are disclosed. A consensus CHIKV Env protein which includes CHIKV E1 consensus protein, CHIKV E2 consensus protein, CHIKV E3 consensus protein, or fragments and homologues thereof and nucleic acid molecules that encode the same are also disclosed. Compositions and recombinant vaccines comprising CHIKV consensus proteins, and methods of using them are disclosed.

摘要翻译： 公开了共识CHIKV E1蛋白，共有CHIKV E2蛋白，共有CHIKV衣壳蛋白或其片段和同系物，以及编码它们的核酸分子。 还公开了包括CHIKV E1共有蛋白，CHIKV E2共有蛋白，CHIKV E3共有蛋白或其片段和同系物以及编码它们的核酸分子的共识CHIKV Env蛋白。 公开了包含CHIKV共有蛋白质的组合物和重组疫苗及其使用方法。

公开(公告)号：US20140127244A1

公开(公告)日：2014-05-08

申请号：US14054621

申请日：2013-10-15

申请人： Arnold I. Levinson ,  Sandra Calarota ,  David B. Weiner ,  Miguel Otero

发明人： Arnold I. Levinson ,  Sandra Calarota ,  David B. Weiner ,  Miguel Otero

IPC分类号： A61K39/00 ,  A61K39/08

CPC分类号： A61K39/0005 ,  A61K39/00 ,  A61K39/08 ,  A61K2039/505 ,  A61K2039/53 ,  C07K16/00 ,  C07K2317/52 ,  C07K2318/10 ,  C07K2319/55

摘要： Nucleic acid molecules that encode a protein comprising at least one epitope of membrane IgE free of epitopes present on the serum IgE, including proteins that further comprise non-IgE T cell helper epitope are disclosed. Vaccines, vectors and host cells that comprise such nucleic acid molecules are disclosed. Isolated proteins, including haptenized proteins, comprising at least one epitope of membrane IgE free of epitopes present on the serum IgE, including proteins that further comprise non-IgE T cell helper epitope are disclosed. Vaccines that comprise and methods of making such proteins and antibodies that specifically bind to such proteins are disclosed. Vaccines that comprise killed or inactivated cells or particles are disclosed. Methods of treating and preventing IgE mediated allergic disease or condition are disclosed.

摘要翻译： 公开了编码蛋白质的核酸分子，其包含不含存在于血清IgE上的表位的膜IgE的至少一个表位，包括进一步包含非IgE T细胞辅助表位的蛋白质。 公开了包含这种核酸分子的疫苗，载体和宿主细胞。 公开了分离的蛋白质，包括半抗原蛋白，其包含至少一个不含存在于血清IgE上的表位的膜IgE表位，包括进一步包含非IgE T细胞辅助表位的蛋白质。 公开了包含制备这种蛋白质和特异性结合这些蛋白质的抗体的方法的疫苗。 公开了包含杀死或灭活的细胞或颗粒的疫苗。 公开了治疗和预防IgE介导的过敏性疾病或病症的方法。

公开(公告)号：US20130273112A1

公开(公告)日：2013-10-17

申请号：US13876148

申请日：2011-09-27

申请人： David B. Weiner ,  Bernadette Ferraro ,  Jian Yan ,  Niranjan Y. Sardesai

发明人： David B. Weiner ,  Bernadette Ferraro ,  Jian Yan ,  Niranjan Y. Sardesai

IPC分类号： A61K39/015 ,  A61K38/20

摘要： Provided herein is consensus amino acid sequences of P. falciparum (P.f.) proteins and their encoding sequences, as well as expression constructs expressing the sequences. Also provided herein are methods for generating an immune response against P. falciparum using the expression constructs provided herein.

摘要翻译： 本文提供恶性疟原虫（P.f.）蛋白及其编码序列的共有氨基酸序列以及表达序列的表达构建体。 本文还提供了使用本文提供的表达构建体来产生针对恶性疟原虫的免疫应答的方法。

公开(公告)号：US20130004529A1

公开(公告)日：2013-01-03

申请号：US13458013

申请日：2012-04-27

申请人： David B. Weiner ,  Jian Yan ,  Dominick Laddy

发明人： David B. Weiner ,  Jian Yan ,  Dominick Laddy

IPC分类号： A61K39/00 ,  C07K14/155 ,  A61P37/04 ,  A61P31/18 ,  A61P31/20 ,  A61P31/14 ,  A61P31/16 ,  A61K39/21 ,  C07K2/00 ,  C07K14/025 ,  C07K14/08 ,  C12N9/12 ,  C07K14/11 ,  A61K39/29 ,  A61K39/145 ,  A61K39/12 ,  C12N15/54 ,  C12N15/37 ,  C12N15/44 ,  C12N15/40 ,  C12N15/63 ,  C12N7/04 ,  C12N5/09 ,  A61P35/00 ,  C12N15/49

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/523 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/55538 ,  A61K2039/585 ,  C12N7/00 ,  C12N2710/20034 ,  C12N2740/16034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2740/16322 ,  C12N2740/16334 ,  C12N2760/16134 ,  C12N2770/24234

摘要： Improved anti-HIV immunogens and nucleic acid molecules that encode them are disclosed. Immunogens disclosed include those having consensus sequences for HIV Subtype A Envelope protein, those having consensus sequences for HIV Subtype B Envelope protein, those having consensus sequences for HIV Subtype C Envelope protein, those having consensus sequences for HIV Subtype D Envelope protein, those having consensus sequences for HIV Subtype B consensus Nef-Rev protein, and those having consensus sequences form HIV Gag protein subtypes A, B, C and D. Improved anti-HPV immunogens and nucleic acid molecules that encode them; improved anti-HCV immunogens and nucleic acid molecules that encode them; improved hTERT immunogens and nucleic acid molecules that encode them; and improved anti-Influenza immunogens and nucleic acid molecules that encode them are disclosed as well methods of inducing an immune response in an individual against HIV, HPV, HCV, hTERT and Influenza are disclosed.

摘要翻译： 公开了改进的抗HIV免疫原和编码它们的核酸分子。 所公开的免疫原包括具有HIV亚型A包膜蛋白的共有序列的那些，对于HIV亚型B包膜蛋白具有共有序列的那些，对于HIV亚型C包膜蛋白具有共有序列的那些，对于HIV亚型D包膜蛋白具有共有序列的那些 HIV亚型B共有序列Nef-Rev蛋白和具有共有序列的序列形成HIV Gag蛋白亚型A，B，C和D.改进的抗HPV免疫原和编码它们的核酸分子; 改进的抗HCV免疫原和编码它们的核酸分子; 改进的hTERT免疫原和编码它们的核酸分子; 公开了改进的抗流感免疫原和编码它们的核酸分子，以及在个体中针对HIV，HPV，HCV，hTERT和流感诱导免疫应答的方法。