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139 条记录 (耗时 0.038 秒)

    Method and device for sample preparation
    41.
    发明授权
    Method and device for sample preparation 有权
    样品制备方法和装置

    公开(公告)号：US07722820B2

    公开(公告)日：2010-05-25

    申请号：US11285531

    申请日：2005-11-21

    申请人： Douglas T. Gjerde ,  Allen Burge ,  Ronald Jones ,  Mark Abel

    发明人： Douglas T. Gjerde ,  Allen Burge ,  Ronald Jones ,  Mark Abel

    IPC分类号： B01D15/08

    CPC分类号： G01N1/40 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3244 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/603 ,  G01N30/6065 ,  G01N2035/1053 ,  Y10T436/25375 ,  Y10T436/255 ,  B01D15/3804

    摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.

    摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床，通常在两个玻璃料之间。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了以疏水和/或亲水膜为玻璃料，和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。

    Method for extracting an analyte
    43.
    发明申请
    Method for extracting an analyte 审中-公开
    提取分析物的方法

    公开(公告)号：US20080145950A1

    公开(公告)日：2008-06-19

    申请号：US12002242

    申请日：2007-12-13

    申请人： Douglas T. Gjerde

    发明人： Douglas T. Gjerde

    IPC分类号： G01N1/28

    CPC分类号： B01D15/3809 ,  B01D15/3828 ,  B01J2220/64 ,  G01N30/02 ,  Y10T436/255 ,  B01D15/3804

    摘要： The instant invention provides a multi-step method for using an extraction device to extract an analyte from a liquid sample. In particular, the invention provides methods for performing a pre-elution wash step to increase the efficiency of the elution step and the yield and concentration of the analyte.

    摘要翻译： 本发明提供了使用提取装置从液体样品中提取分析物的多步骤方法。 特别地，本发明提供了进行洗脱前洗涤步骤以提高洗脱步骤的效率以及分析物的产率和浓度的方法。

    Column for DNA separation by matched ion polynucleotide chromatography
    44.
    发明授权
    Column for DNA separation by matched ion polynucleotide chromatography 失效

    公开(公告)号：US06372142B1

    公开(公告)日：2002-04-16

    申请号：US09501775

    申请日：2000-02-10

    申请人： Douglas T. Gjerde ,  Robert M. Haefele ,  Karl H. Hecker ,  Raquel R. Roque

    发明人： Douglas T. Gjerde ,  Robert M. Haefele ,  Karl H. Hecker ,  Raquel R. Roque

    IPC分类号： B01D1508

    CPC分类号： B01D15/325 ,  B01D15/366 ,  B01D15/424 ,  C12N15/101

    摘要： An improved separation column and method for separating a mixture of double stranded DNA fragments by Matched Ion Polynucleotide Chromatography. The cylindrical column has an ID greater than about 5 mm and contains polymer beads. The beads have an average diameter of 1 to 100 microns and are unsubstituted polymer beads or are polymer beads substituted with a hydrocarbon moiety having from 1 to 1,000,000 carbons. The preferred beads are characterized by being substantially free from multivalent cations which are free to bind with DNA. The improved column provides enhanced separation of DNA fragments with sizes ranging from about 100 to 20,000 base pairs. The column also provides enhanced separation of heteroduplex and homoduplex DNA molecules in a mutation detection procedure in which the chromatography is performed under conditions effecting partial denaturation of DNA at a site of mismatched base pairs.

    Apparatus for performing polynucleotide separations using liquid chromatography
    45.
    发明授权
    Apparatus for performing polynucleotide separations using liquid chromatography 有权
    使用液相色谱进行多核苷酸分离的装置

    公开(公告)号：US6030527A

    公开(公告)日：2000-02-29

    申请号：US350737

    申请日：1999-07-09

    申请人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    发明人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    IPC分类号： B01D15/08

    CPC分类号： B01D15/08

    摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

    摘要翻译： 具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。 处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。 或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。 多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。 基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

    Method for performing polynucleotide separations using liquid chromatography
    46.
    发明授权
    Method for performing polynucleotide separations using liquid chromatography 失效
    使用液相色谱进行多核苷酸分离的方法

    公开(公告)号：US6017457A

    公开(公告)日：2000-01-25

    申请号：US80547

    申请日：1998-05-18

    申请人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    发明人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    IPC分类号： B01D15/08 ,  B01D15/12 ,  B01D15/36 ,  B01D15/38 ,  B01J45/00 ,  C12N15/10 ,  G01N30/14 ,  G01N30/52

    CPC分类号： C12N15/101 ,  B01D15/12 ,  B01D15/366 ,  B01D15/38 ,  B01J45/00 ,  G01N2030/528 ,  G01N30/14

    摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

    摘要翻译： 具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。 处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。 或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。 多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。 基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

    System and method for performing nucleic acid separations using liquid chromatography
    47.
    发明授权
    System and method for performing nucleic acid separations using liquid chromatography 失效
    使用液相色谱进行核酸分离的系统和方法

    公开(公告)号：US5772889A

    公开(公告)日：1998-06-30

    申请号：US748376

    申请日：1996-11-13

    申请人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    发明人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    IPC分类号： B01D15/12 ,  B01D15/36 ,  B01D15/38 ,  B01J45/00 ,  C12N15/10 ,  G01N30/14 ,  G01N30/52 ,  B01D15/08

    CPC分类号： B01D15/366 ,  B01D15/12 ,  B01D15/38 ,  B01J45/00 ,  C12N15/101 ,  G01N2030/528 ,  G01N30/14

    摘要： Improved liquid chromatography systems having components made of titanium, coated stainless steel, or organic polymeric material are useful in the separation of nucleic acid fragments, particularly large fragments of double-stranded nucleic acids, by ion pairing reverse phase chromatography. The titanium, coated stainless steel, or polymeric components do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column may be employed to remove multivalent ions from the system. The multivalent cation capture resin may be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of nucleic acid fragments into fractions based on their molecular weight by ion pairing reverse phase chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

    摘要翻译： 具有由钛，涂层不锈钢或有机聚合材料制成的部件的改进的液相色谱系统可用于通过离子配对反相色谱法分离核酸片段，特别是双链核酸的大片段。 钛，涂层不锈钢或聚合物组分不会将多价阳离子释放到流过色谱系统的水溶液中。 或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。 多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。 通过离子对反相色谱法和回旋分离色谱法分离基于其分子量的核酸片段混合物的新方法利用上述液相色谱系统。

    Columns for isolation, detection and use of biological cells
    48.
    发明授权
    Columns for isolation, detection and use of biological cells 有权

    公开(公告)号：US11097207B2

    公开(公告)日：2021-08-24

    申请号：US16292271

    申请日：2019-03-04

    申请人： Douglas T. Gjerde

    发明人： Douglas T. Gjerde

    IPC分类号： B01D15/22 ,  C07K1/22 ,  B01D15/38 ,  G01N1/34 ,  B01D15/18 ,  G01N1/40 ,  B01D15/14 ,  G01N35/04

    摘要： This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.