公开(公告)号：US10570250B2

公开(公告)日：2020-02-25

申请号：US16107818

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  H01L51/00 ,  C07F7/08 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58 ,  C09K11/06 ,  G01N33/545

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US20190242912A1

公开(公告)日：2019-08-08

申请号：US16298810

申请日：2019-03-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel Beacham ,  Kyle Gee

IPC: G01N33/84 ,  G01N33/68 ,  C12Q1/02

CPC classification number: G01N33/84 ,  C12Q1/02 ,  G01N33/6872 ,  G01N2333/705

Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

公开(公告)号：US20180355098A1

公开(公告)日：2018-12-13

申请号：US16107818

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  H01L51/00 ,  G01N33/545 ,  C09K11/06 ,  G01N33/58 ,  G01N33/533 ,  C08L65/00 ,  C07F7/08

CPC classification number: C08G61/123 ,  C07F7/0816 ,  C07F7/083 ,  C08G2261/122 ,  C08G2261/124 ,  C08G2261/1424 ,  C08G2261/145 ,  C08G2261/1644 ,  C08G2261/1646 ,  C08G2261/3142 ,  C08G2261/3244 ,  C08G2261/334 ,  C08G2261/344 ,  C08G2261/411 ,  C08G2261/522 ,  C08G2261/5222 ,  C08G2261/72 ,  C08G2261/94 ,  C08L65/00 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/1491 ,  G01N33/533 ,  G01N33/545 ,  G01N33/582 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/0094

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US20180202936A1

公开(公告)日：2018-07-19

申请号：US15923262

申请日：2018-03-16

Applicant: Life Technologies Corporation

Inventor： Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli ,  Lai-Qiang Ying ,  Bruce Branchaud

IPC: G01N21/64 ,  C09B57/00 ,  C09B23/06 ,  C09B23/08

CPC classification number: G01N21/6486 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

Abstract: The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

公开(公告)号：US09910051B2

公开(公告)日：2018-03-06

申请号：US14455550

申请日：2014-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel Beacham ,  Jeffrey Dzubay ,  Kyle Gee ,  Vladimir Martin ,  Aleksey Rukavishnikov

IPC: G01N33/84 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  G01N31/22 ,  C09B11/24 ,  G01N33/50 ,  G01N33/58 ,  G01N33/80 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07D209/14 ,  C09K11/06 ,  C07F5/02

CPC classification number: G01N33/84 ,  C07D209/14 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/24 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  C09K11/06 ,  G01N31/22 ,  G01N33/5005 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/582 ,  G01N33/80 ,  G01N2333/245 ,  Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

公开(公告)号：US09846168B2

公开(公告)日：2017-12-19

申请号：US15152346

申请日：2016-05-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Vladimir Martin

IPC: G01N21/76 ,  G01N33/84 ,  C07C229/18 ,  C07D221/14 ,  C07D265/38 ,  C07D311/82 ,  C09B5/60 ,  C09B5/62 ,  C09B11/06 ,  C09K11/06 ,  C07D209/14 ,  C07D311/90 ,  G01N33/20 ,  C07C311/44 ,  C07K14/195 ,  C07K14/435 ,  C09B11/08 ,  C09B19/00 ,  C09B57/08 ,  C07D413/04

CPC classification number: G01N33/84 ,  C07C229/18 ,  C07C311/44 ,  C07D209/14 ,  C07D221/14 ,  C07D265/38 ,  C07D311/82 ,  C07D311/90 ,  C07D413/04 ,  C07K14/195 ,  C07K14/43595 ,  C09B5/60 ,  C09B5/62 ,  C09B11/06 ,  C09B11/08 ,  C09B19/00 ,  C09B19/005 ,  C09B57/08 ,  C09K11/06 ,  C09K2211/1014 ,  G01N33/20 ,  G01N2458/00

Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.

公开(公告)号：US09389183B2

公开(公告)日：2016-07-12

申请号：US13793517

申请日：2013-03-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

IPC: C07D311/82 ,  C07D471/06 ,  C07F9/655 ,  G01N21/64 ,  C07F5/02 ,  C07F9/54 ,  C09B11/24 ,  G01N33/52

CPC classification number: G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F5/022 ,  C07F9/5442 ,  C07F9/65522 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/10 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/52 ,  G01N2021/6439 ,  Y10T436/143333

Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.

Abstract translation: 本公开涉及能够检测含有醛和酮基的分析物的荧光席夫碱形成染料。 染料含有亲核肼基附属物，并能够原位结合和检测分析物。

公开(公告)号：US20160025713A1

公开(公告)日：2016-01-28

申请号：US14876782

申请日：2015-10-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Courtenay Hart-Kerndt ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

IPC: G01N33/52 ,  C07F5/02 ,  C07D311/16 ,  G01N33/58

CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

公开(公告)号：US09140706B2

公开(公告)日：2015-09-22

申请号：US13972081

申请日：2013-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Evan Antoulinakis ,  Kyle Gee ,  Aleksey Rukavishnikov

IPC: C07D311/80 ,  C07D311/82 ,  C07D311/88 ,  G01N33/58 ,  C07D209/14 ,  C07D401/10 ,  G01N33/532 ,  C09B11/26 ,  C09B45/00

CPC classification number: G01N33/58 ,  C07D209/14 ,  C07D209/18 ,  C07D311/18 ,  C07D311/82 ,  C07D401/10 ,  C07D493/14 ,  C07D495/22 ,  C07F5/022 ,  C09B11/24 ,  C09B11/26 ,  C09B23/04 ,  C09B23/08 ,  C09B45/00 ,  C09B56/16 ,  G01N33/532 ,  G01N33/582

Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.

Abstract translation: 本公开涉及能够将报告分子与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有以下通式：其中在整个申请中描述了变量。

公开(公告)号：US20130337536A1

公开(公告)日：2013-12-19

申请号：US13972081

申请日：2013-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Evan Antoulinakis ,  Kyle Gee ,  Aleksey Rukavishnikov

IPC: G01N33/58 ,  C09B11/26 ,  C09B45/00

CPC classification number: G01N33/58 ,  C07D209/14 ,  C07D209/18 ,  C07D311/18 ,  C07D311/82 ,  C07D401/10 ,  C07D493/14 ,  C07D495/22 ,  C07F5/022 ,  C09B11/24 ,  C09B11/26 ,  C09B23/04 ,  C09B23/08 ,  C09B45/00 ,  C09B56/16 ,  G01N33/532 ,  G01N33/582

Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.