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64 条记录 (耗时 0.021 秒)

    Process for the microbial production of alcoholoxidase
    42.
    发明授权
    Process for the microbial production of alcoholoxidase 失效
    藻酸酶微生物生产工艺

    公开(公告)号：US4250261A

    公开(公告)日：1981-02-10

    申请号：US55533

    申请日：1979-07-09

    申请人： Lothar Eggeling ,  Manfred Paschke ,  Hermann Sahm

    发明人： Lothar Eggeling ,  Manfred Paschke ,  Hermann Sahm

    IPC分类号： C12N9/04 ,  C12R1/78

    CPC分类号： C12N9/0006 ,  Y10S435/93

    摘要： A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.

    摘要翻译： 一种生产醇氧化酶的方法，其中将多形汉逊酵母（Hansenula polymorpha yeast）培养在含有无机营养盐的营养肉汤中，其中磷酸盐，硝酸盐或钾以25至45℃的温度存在于细胞生长限制浓度中。 pH为4.0至6.0，氧气作为空气和氧气的混合物供应。 根据本发明，在营养液中以0.03-0.15-1的比例，在肉汤中以0.1至1.0％的浓度加入由甘油，山梨糖醇或木糖组成的有机底物，对醇醇氧化酶具有抑制作用。

    Process for obtaining sorbitol and gluconic acid or gluconate usingZymomonas mobilis
    43.
    发明授权
    Process for obtaining sorbitol and gluconic acid or gluconate usingZymomonas mobilis 失效
    获得硫代酸和葡萄糖酸或葡萄糖的方法

    公开(公告)号：US5190869A

    公开(公告)日：1993-03-02

    申请号：US706333

    申请日：1991-05-28

    申请人： Bert Rehr ,  Hermann Sahm

    发明人： Bert Rehr ,  Hermann Sahm

    IPC分类号： C12P7/18 ,  C12P7/58

    CPC分类号： C12P7/58 ,  C12P7/18 ,  Y10S435/822

    摘要： The long-term activity of the biocatalyst for the production of sorbitol and gluconic acid from an aqueous solution of fructose and glucose is considerably improved with the aid of permeabilized cells of Zymomonas mobilis immobilized using .kappa.-carrageenan that has been rigidified and then stabilized by K.sup.+ ions. Rigidification of .kappa.-carrageenan is preferably carried out by treatment with glutaraldehyde alone or by treatment with polyethyleneimine or hexamethylenediamine and subsequent exposure to glutaraldehyde. A buffer-free solution is preferred and the pH is maintained by adding Ca.sup.++ ions while simultaneously precipitating gluconic acid.

    摘要翻译： 借助于使用κ-卡拉胶来固定的运动发酵单胞菌的透化细胞，通过K + - 角叉菜胶固化，然后通过K +稳定化，生物催化剂从果糖和葡萄糖水溶液中生产山梨糖醇和葡萄糖酸的长期活性显着提高 离子。 κ-卡拉胶的刚性优选通过单独使用戊二醛处理或通过用聚乙烯亚胺或六亚甲基二胺处理，随后暴露于戊二醛进行。 优选无缓冲溶液，通过加入Ca ++离子同时沉淀葡萄糖酸来维持pH。

    Secretion of outer membrane proteins of gram-negative bacteria by means of gram-positive host organisms
    45.
    发明授权
    Secretion of outer membrane proteins of gram-negative bacteria by means of gram-positive host organisms 失效
    通过革兰氏阳性宿主生物分泌革兰氏阴性细菌的外膜蛋白

    公开(公告)号：US5858727A

    公开(公告)日：1999-01-12

    申请号：US676378

    申请日：1996-07-18

    申请人： Jochen Meens ,  Michael Klose ,  Hermann Sahm ,  Roland Freudl

    发明人： Jochen Meens ,  Michael Klose ,  Hermann Sahm ,  Roland Freudl

    IPC分类号： A61K35/74 ,  A61K38/00 ,  A61K39/02 ,  A61P31/04 ,  C07H21/04 ,  C07K14/245 ,  C07K14/31 ,  C12N1/21 ,  C12N9/20 ,  C12N15/09 ,  C12N15/31 ,  C12N15/62 ,  C12P21/02 ,  G01N33/569 ,  C07K14/195

    CPC分类号： C12N9/20 ,  C07K14/245 ,  C12N15/625 ,  G01N33/56911 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/036 ,  Y10S530/825

    摘要： The invention relates to a process for producing outer membrane proteins of gram-negative bacteria by gram-positive host cells. To this end, a gene structure containing a gene coded for an outer membrane protein is constructed; in front of this gene there is a pro-gene sequence which codes for a propeptide of an export protein of a gram-positive bacterium. In front of the pro-gene sequence there is a pre-gene sequence which codes for a signal peptide of an export protein of a gram-positive bacterium. After the cloning of a gene structure thus constructed into a vector and transformation into a gram-positive bacterium, the outer membrane protein is expressed with a signal peptide and propeptide, transported through the membrane of the gram-positive host cell and released into the test of the culture.

    摘要翻译： PCT No.PCT / DE94 / 01394 Sec。 371日期：1996年7月18日 102（e）日期1996年7月18日PCT 1994年11月21日PCT PCT。 WO95 / 20048 PCT出版物 日期1995年7月27日本发明涉及通过革兰氏阳性宿主细胞产生革兰氏阴性细菌的外膜蛋白的方法。 为此，构建了含有外膜蛋白编码基因的基因结构体; 在该基因前面有一个pro-gene序列，其编码革兰氏阳性细菌的出口蛋白质的前肽。 在前基因序列前面有一个前基因序列，其编码革兰氏阳性细菌的出口蛋白质的信号肽。 克隆由此构建成载体并转化成革兰氏阳性细菌的基因结构后，外膜蛋白用信号肽和前肽表达，通过革兰氏阳性宿主细胞的膜转运并释放到测试中 的文化。

    Process for obtaining sorbitol and gluconic acid or gluconate
    47.
    发明授权
    Process for obtaining sorbitol and gluconic acid or gluconate 失效
    获得硫醇和葡萄糖酸或葡萄糖的方法

    公开(公告)号：US5102795A

    公开(公告)日：1992-04-07

    申请号：US606821

    申请日：1990-10-31

    申请人： Bert Rehr ,  Hermann Sahm

    发明人： Bert Rehr ,  Hermann Sahm

    IPC分类号： C12P7/18 ,  C12P7/58

    CPC分类号： C12P7/18 ,  C12P7/58 ,  Y10S435/822

    摘要： A process for obtaining sorbitol and gluconic acid or gluconate from aqueous glucose/fructose mixtures by using Zymomonas mobilis cells which have been permeabilized by treatment with cationic surfactant is disclosed. The permeabilized cells are preferably obtained by treatment with long-chain quaternary alkylammonium salts such as, in particular, CTAB, Dodigen or Bardac. Surfactant concentrations of 0.1 to 0.3% and a treatment time of 1 to 10 minutes at room temperature are expedient. The cell concentrations preferably used for the fermentation are 20 to 80 g/1 dry matter of permeabilized cells.

    摘要翻译： 公开了通过使用通过用阳离子表面活性剂处理透化的运动发酵单胞菌细胞从水性葡萄糖/果糖混合物中获得山梨糖醇和葡萄糖酸或葡萄糖酸盐的方法。 透化细胞优选通过用长链季铵烷基铵盐，特别是CTAB，Dodigen或Bardac处理来获得。 表面活性剂浓度为0.1〜0.3％，室温下处理时间为1〜10分钟为宜。 优选用于发酵的细胞浓度为透化细胞的20〜80g / l干物质。

    Process for obtaining sorbitol and gluconic acid by fermentation, and cell material suitable for this purpose
    48.
    发明授权
    Process for obtaining sorbitol and gluconic acid by fermentation, and cell material suitable for this purpose 失效
    通过发酵获得山梨糖醇和葡萄糖酸的方法，以及适用于该目的的细胞材料

    公开(公告)号：US5017485A

    公开(公告)日：1991-05-21

    申请号：US448334

    申请日：1989-12-11

    申请人： Stephanie Bringer-Meyer ,  Hermann Sahm

    发明人： Stephanie Bringer-Meyer ,  Hermann Sahm

    IPC分类号： C12P7/18 ,  C12P7/58

    CPC分类号： C12P7/18 ,  C12P7/58 ,  Y10S435/822

    摘要： A process is disclosed for obtaining sorbitol and gluconic acid, or gluconate, by fermentation in aqueous glucose/fructose mixtures. Zymomonas mobilis cells are used which have been permeabilized by the freeze technique and which have preferably been obtained by freezing cell centrifugates of pH 6 to 7 at about -20.degree. C., and thawing at room temperature. Cell concentrations of 20 to 60 g of cell dry matter of permeabilized cells/l are preferably used for the fermentation. By using cells which have been permeabilized by freezing and thawing, a considerably higher conversion rate of glucose/fructose into sorbitol and gluconic acid, or gluconate, is achieved.

    摘要翻译： 公开了通过在水性葡萄糖/果糖混合物中发酵获得山梨糖醇和葡萄糖酸或葡萄糖酸盐的方法。 使用通过冷冻技术透化的运动发酵单胞菌细胞，其优选通过在约-20℃下冷冻pH6-7的细胞离心液并在室温下解冻获得。 细胞浓度为20〜60g的透化细胞/ l细胞干物质优选用于发酵。 通过使用通过冷冻和解冻而透化的细胞，实现了相当高的葡萄糖/果糖转化为山梨醇和葡糖酸或葡萄糖酸的转化率。