Methods For Enhancing Segregation of Transgenes in Plants and Compositions Thereof
    49.
    发明申请
    Methods For Enhancing Segregation of Transgenes in Plants and Compositions Thereof 审中-公开
    用于增强植物及其组合物中转基因分离的方法

    公开(公告)号:US20160024512A1

    公开(公告)日:2016-01-28

    申请号:US14862734

    申请日:2015-09-23

    Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

    Abstract translation: 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因,阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体,将植物细胞再生成植物并鉴定独立的转基因基因座,其中可选择标记基因或转基因元件可以在后代中分离。

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