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41.发明申请公开(公告)号：US20150052642A1
公开(公告)日：2015-02-19
申请号：US14455747
申请日：2014-08-08
Applicant: Monsanto Technology LLC
Inventor： Xudong Ye , Michael W. Petersen , Larry A. Gilbertson , David Walters , Susan J. Johnson , Shihshieh Huang , Paul S. Chomet
CPC classification number: C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
Abstract translation: 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。
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公开(公告)号：US11840700B2
公开(公告)日：2023-12-12
申请号：US15217695
申请日：2016-07-22
Applicant: Monsanto Technology LLC
Inventor： Timothy K. Ball , Artem G. Evdokimov , Larry A. Gilbertson , Victor M. Guzov , Jeffrey A. Haas , Qing Huai , Sergey Ivashuta , Melissa M. Kemp , Yifei Kong , Thomas M. Malvar , Byron V. Olsen , Parthasarathy Ramaseshadri , Brian E. Weiner
IPC: C12N15/82 , A01N37/44 , C07K14/325 , A01N37/46
CPC classification number: C12N15/8286 , A01N37/44 , A01N37/46 , C07K14/325 , Y02A40/146
Abstract: Pesticidal proteins are provided that combine two or more modes of action into a single molecule. These pesticidal proteins therefor exhibit greater efficacy and/or durability of resistance, for the purposes of pest control, and can be utilized for pest control by provision in the diet of a pest organism, or by topical application to crop plants and/or pests. Methods and compositions for producing and using such proteins are also provided.
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公开(公告)号：US11492638B2
公开(公告)日：2022-11-08
申请号：US15709291
申请日：2017-09-19
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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公开(公告)号：US20220033833A1
公开(公告)日：2022-02-03
申请号：US17276455
申请日：2019-10-01
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Andrei Y. Kouranov , Vladimir A. Sidorov
Abstract: The invention provides novel methods and compositions for introduction, transfer or delivery of one or more biomolecules into wounded recipient plant cell(s). Methods for production of a wounded recipient cell culture and the creation of one or more mutations, edits, transgenic insertions, or other genetic changes in the recipient cell(s) are also provided. Product cells produced by such methods, and resulting cells and regenerated plants, plant parts, and progeny plants are further provided. Molecular and genetic analyses, analysis of phenotypes and traits, and use of screenable and selection markers, are also provided to confirm transfer of the biomolecule in to the recipient cell(s) and generation of the mutation, edit, transgenic insertion, or other genetic change in the recipient cell(s), and/or progeny thereof, and in plants or plant parts developed or regenerated from the foregoing.
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公开(公告)号：US20210207155A1
公开(公告)日：2021-07-08
申请号：US17206113
申请日：2021-03-18
Applicant: Monsanto Technology LLC
Inventor： Edwards Allen , Larry A. Gilbertson , Sergey I. Ivashuta , James K. Roberts
IPC: C12N15/82
Abstract: This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.
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Patent Agency Ranking
公开(公告)号：US20190211346A1
公开(公告)日：2019-07-11
申请号：US16242887
申请日：2019-01-08
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Michael W. Petersen
IPC: C12N15/82
Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
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公开(公告)号：US10240165B2
公开(公告)日：2019-03-26
申请号：US15376495
申请日：2016-12-12
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Shihshieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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公开(公告)号：US10167484B2
公开(公告)日：2019-01-01
申请号：US15130684
申请日：2016-04-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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公开(公告)号：US09856485B2
公开(公告)日：2018-01-02
申请号：US14337932
申请日：2014-07-22
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Shihshieh Huang , Thomas Malvar
CPC classification number: C12N15/8213 , C12N15/63 , C12N15/8217 , C12N15/8218 , C12N15/8243
Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
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公开(公告)号：US20170253937A1
公开(公告)日：2017-09-07
申请号：US15376495
申请日：2016-12-12
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Shihshieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
CPC classification number: C12N15/8265 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8222 , C12N15/8231 , C12N15/8234 , C12N15/8261 , C12N15/8263 , C12N15/8267 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12N15/8294
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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