公开(公告)号：US20240261472A1

公开(公告)日：2024-08-08

申请号：US18567448

申请日：2022-06-06

Applicant: President and Fellows of Harvard College

Inventor： Daniel S. REYNOLDS ,  Jennifer A. Lewis

IPC: A61L27/48 ,  A61L27/54 ,  B29C64/106 ,  B29C64/40 ,  B33Y10/00 ,  B33Y70/00 ,  C09D11/03 ,  C09D11/04 ,  C09D11/08 ,  C09D11/14

CPC classification number: A61L27/48 ,  A61L27/54 ,  B29C64/106 ,  B29C64/40 ,  C09D11/03 ,  C09D11/04 ,  C09D11/08 ,  C09D11/14 ,  B33Y10/00 ,  B33Y70/00

Abstract: A bioink for extrusion-based printing includes an extracellular matrix (ECM) precursor comprising an uncrosslinked polymer, and sacrificial microparticles dispersed in the ECM precursor. The sacrificial microparticles have a melting temperature above a crosslinking temperature of the uncrosslinked polymer. A method of fabricating a tissue/organ model or therapeutic construct comprises extruding a bioink comprising a first ECM precursor and first sacrificial microparticles through a nozzle moving relative to a deposition bath, and depositing an extruded filament comprising the bioink into the deposition bath as the nozzle moves. After deposition, the first ECM precursor is crosslinked to form a first ECM material, and after the crosslinking, the first sacrificial microparticles are melted to form pores in the first ECM material. The pores may have a width or diameter comparable to that of individual cells.

公开(公告)号：US12005631B2

公开(公告)日：2024-06-11

申请号：US17982877

申请日：2022-11-08

Applicant: President and Fellows of Harvard College

Inventor： Natalie M. Larson ,  Jochen Mueller ,  Jennifer A. Lewis

IPC: B29C64/118 ,  B29C64/209 ,  B29C64/255 ,  B29C64/264 ,  B29C64/336 ,  B33Y10/00 ,  B33Y30/00

CPC classification number: B29C64/118 ,  B29C64/209 ,  B29C64/255 ,  B29C64/264 ,  B29C64/336 ,  B33Y10/00 ,  B33Y30/00

Abstract: A printhead comprises a plurality of ink cartridges and a nozzle, where the nozzle and the ink cartridges are configured to rotate together about an axis during printing. The nozzle includes a nozzle body comprising an inlet end, an outlet end, and one or more internal passageways extending through the nozzle body from the inlet end to the outlet end. The one or more internal passageways terminate at one or more outlets at or near the outlet end. The nozzle also includes plurality of nozzle inlets at the inlet end for delivery of flowable inks into the internal passageways, where each nozzle inlet is in fluid communication with a dispensing end of one of the ink cartridges.

公开(公告)号：US20230049026A1

公开(公告)日：2023-02-16

申请号：US17743804

申请日：2022-05-13

Applicant: President and Fellows of Harvard College ,  Trustees of Boston University

Inventor： Arda Kotikian ,  Jennifer A. Lewis ,  Javier M. Morales Ferrer ,  John William Boley

IPC: B29C64/118 ,  B33Y10/00 ,  B33Y80/00

Abstract: A method of forming an innervated liquid crystal elastomer (iLCE) actuator comprises extruding a filament through a nozzle moving relative to a substrate, where the filament has a core-shell structure including a shell comprising a liquid crystal elastomer surrounding a core configured to induce a nematic-to-isotropic transition of the liquid crystal elastomer. The filament is subjected to UV curing as the filament is extruded, and the filament is deposited on the substrate as the nozzle moves. A director of the liquid crystal elastomer is aligned with a print path of the nozzle, and a 3D printed architecture configured for actuation is formed.

公开(公告)号：US11559607B2

公开(公告)日：2023-01-24

申请号：US16330974

申请日：2017-09-06

Applicant: President and Fellows of Harvard College

Inventor： David B. Kolesky ,  Kimberly A. Homan ,  Jennifer A. Lewis ,  Yen-Chih Lin

IPC: A61L27/38 ,  A61M1/34 ,  A61M1/36 ,  A01N1/02

Abstract: Described are devices and methods for use in connection with organ replacement or organ assist therapy in a patient.

公开(公告)号：US11498332B2

公开(公告)日：2022-11-15

申请号：US16320746

申请日：2017-07-24

Applicant: President and Fellows of Harvard College

Inventor： Daniele Foresti ,  Jennifer A. Lewis ,  Armand Kurum

IPC: B41J2/14 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  B29C64/00 ,  A23P20/20 ,  A23G1/50 ,  A61L27/00 ,  B41M5/00 ,  C09D11/50 ,  B29C64/112 ,  B29C64/209 ,  A23G1/54 ,  A61L27/24 ,  A61L27/38 ,  A61L27/54 ,  C09D11/52 ,  A23P20/25

Abstract: A method of acoustophoretic printing comprises generating an acoustic field at a first end of an acoustic chamber fully or partially enclosed by sound-reflecting walls. The acoustic field interacts with the sound-reflecting walls and travels through the acoustic chamber. The acoustic field is enhanced in a chamber outlet at a second end of the acoustic chamber. An ink is delivered into a nozzle positioned within the acoustic chamber. The nozzle has a nozzle opening projecting into the chamber outlet. The ink travels through the nozzle and is exposed to the enhanced acoustic field at the nozzle opening, and a predetermined volume of the ink is ejected from the nozzle opening and out of the acoustic chamber.

公开(公告)号：US10462907B2

公开(公告)日：2019-10-29

申请号：US14900860

申请日：2014-06-24

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Michael A. Bell ,  Travis A. Busbee ,  John E. Minardi, II

IPC: H05K3/00 ,  H05K3/12 ,  H01L23/13 ,  H01L21/48 ,  H01L23/31 ,  H01L21/56 ,  H01L23/498 ,  H01L23/538 ,  H01L23/00 ,  H05K1/02 ,  H01L25/065 ,  H01L25/00 ,  B33Y80/00 ,  B33Y10/00 ,  H01M4/04 ,  H05K7/02 ,  B29C70/72 ,  H04R25/00 ,  B29C64/00 ,  B29C64/106 ,  H01M4/02 ,  H05K1/18

Abstract: A printed 3D functional part includes a 3D structure comprising a structural material, and at least one functional electronic device is at least partially embedded in the 3D structure. The functional electronic device has a base secured against an interior surface of the 3D structure. One or more conductive filaments are at least partially embedded in the 3D structure and electrically connected to the at least one functional electronic device.

公开(公告)号：US20190242878A1

公开(公告)日：2019-08-08

申请号：US16387323

申请日：2019-04-17

Applicant: Massachusetts Institute of Technology ,  President and Fellows of Harvard College

Inventor： Krystyn J. Van Vliet ,  Anna Jagielska ,  Kimberly Homan ,  Jennifer A. Lewis ,  Travis Alexander Busbee

IPC: G01N33/50 ,  C12M1/34 ,  C12M1/12

CPC classification number: G01N33/5032 ,  C12M25/04 ,  C12M25/14 ,  C12M41/38 ,  C12M41/46 ,  G01N33/5058

Abstract: Aspects of the present invention provide improved methods and apparatus for use in in vitro modeling of the interaction of cells with cellular constructs/parts/axons, including axon mimetics and use of three-dimensional fibers.

公开(公告)号：US20190105622A1

公开(公告)日：2019-04-11

申请号：US16126723

申请日：2018-09-10

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Thomas J. Ober

IPC: B01F15/00 ,  B41J2/21 ,  B01F7/24 ,  B01F3/08 ,  B01F3/12 ,  B01F7/06 ,  B01F13/00 ,  B28C5/02 ,  B29C64/209 ,  B33Y30/00 ,  B41J2/175 ,  B05B1/30 ,  B28C5/12

CPC classification number: B01F15/00389 ,  B01F3/0861 ,  B01F3/0865 ,  B01F3/1221 ,  B01F7/06 ,  B01F7/248 ,  B01F13/0059 ,  B01F13/0064 ,  B01F15/00253 ,  B05B1/30 ,  B28C5/026 ,  B28C5/1292 ,  B29C64/209 ,  B33Y30/00 ,  B41J2/175 ,  B41J2/211

Abstract: A microfluidic printing nozzle for 3D printing may include a mixing chamber, a first inlet for connecting with a first ink source, the first inlet located at a first end of the mixing chamber, and a second inlet for connecting with a second ink source, the second inlet located at the first end of the mixing chamber. An outlet may be located at a second end of the mixing chamber, and a generally cylindrical impeller may be rotatably disposed in the mixing chamber between the first end and the second end. The cylindrical impeller may include an outer surface, and the outer surface of the impeller includes a groove, a protrusion, or both, to facilitate mixing of fluidic inks flowing from the first end to the second end of the mixing chamber.

公开(公告)号：US10151649B2

公开(公告)日：2018-12-11

申请号：US15036937

申请日：2014-11-17

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Joseph T. Muth ,  Daniel M. Vogt ,  Ryan L. Truby ,  Yigit Menguc ,  David B. Kolesky ,  Robert J. Wood

IPC: G01B7/16 ,  G01L1/22 ,  G01L1/18 ,  G01L1/20 ,  G06F3/01

Abstract: A printed stretchable strain sensor comprises a seamless elastomeric body and a strain-sensitive conductive structure embedded in the seamless elastomeric body. The strain-sensitive conductive structure comprises one or more conductive filaments arranged in a continuous pattern. A method of printing a stretchable strain sensor comprises depositing one or more conductive filaments in a predetermined continuous pattern into or onto a support matrix. After the depositing, the support matrix is cured to embed a strain-sensitive conductive structure in a seamless elastomeric body.

公开(公告)号：US10071350B2

公开(公告)日：2018-09-11

申请号：US15564396

申请日：2016-04-07

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Thomas J. Ober

IPC: B41J2/21 ,  B01F3/08 ,  B01F3/12 ,  B01F7/06 ,  B01F15/00 ,  B28C5/02 ,  B01F13/00 ,  B05B1/30 ,  B41J2/175 ,  B01F7/24 ,  B28C5/12

CPC classification number: B01F15/00389 ,  B01F3/0861 ,  B01F3/0865 ,  B01F3/1221 ,  B01F7/06 ,  B01F7/248 ,  B01F13/0059 ,  B01F13/0064 ,  B01F15/00253 ,  B05B1/30 ,  B28C5/026 ,  B28C5/1292 ,  B29C64/209 ,  B33Y30/00 ,  B41J2/175 ,  B41J2/211

Abstract: The present disclosure relates to a device for three-dimensional ink deposition from an impeller-driven active mixing microfluidic printing nozzle. The device is configured to receive a material property associated with the plurality of fluids and receive a structure property of the printing nozzle. The device then determines a threshold relation between a rotating speed Ω of an impeller in the nozzle and a volumetric flow rate Q of fluids that flow through the nozzle based on the material property of the plurality of fluids, the structure property of the printing nozzle. Based on the threshold relation, the device then determines an actual volumetric flow rate of the fluids and actual rotation speed of the impeller.