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115 条记录 (耗时 0.036 秒)

    Factor X deletion mutants and analogues thereof
    41.
    发明授权
    Factor X deletion mutants and analogues thereof 有权
    因子X缺失突变体及其类似物

    公开(公告)号：US06562598B1

    公开(公告)日：2003-05-13

    申请号：US09367777

    申请日：1999-11-18

    申请人： Michele Himmelspach ,  Michael Pfleiderer ,  Falko-Guenter Falkner ,  Johann Eibl ,  Friedrich Dorner ,  Uwe Schlokat

    发明人： Michele Himmelspach ,  Michael Pfleiderer ,  Falko-Guenter Falkner ,  Johann Eibl ,  Friedrich Dorner ,  Uwe Schlokat

    IPC分类号： C12P2106

    CPC分类号： C12N9/6432 ,  A61K38/00 ,  C12Y304/21006

    摘要： Factor X&Dgr; analogues are provided, as well as pharmaceutical preparations containing such analogues and methods of preparing such analogues. The factor X&Dgr; analogues have a deletion of the amino acids Arg180 to Arg234 and a modification in the region of the amino acid sequence between Gly173 and Arg179 of the factor X amino acid sequence. Such analogues can include a processing site not normally present in factor X, thus allowing for selective conversion of the analogue to an active form. The analogues and preparations have utility in the treatment of a number of blood coagulation disorders.

    摘要翻译： 提供因子XDELTA类似物，以及含有此类类似物的药物制剂和制备此类类似物的方法。 因子XDELTA类似物具有氨基酸Arg180至Arg234的缺失和在因子X氨基酸序列的Gly173和Arg179之间的氨基酸序列区域的修饰。 这样的类似物可以包括通常不存在于因子X中的处理位点，从而允许将类似物选择性转化为活性形式。 类似物和制剂可用于治疗许多凝血障碍。

    Perfusion system and a method for the large scale production of virus or virus antigen
    48.
    发明授权
    Perfusion system and a method for the large scale production of virus or virus antigen 失效
    灌注系统和大规模生产病毒或病毒抗原的方法

    公开(公告)号：US5719051A

    公开(公告)日：1998-02-17

    申请号：US357292

    申请日：1994-12-13

    申请人： Wolfgang Mundt ,  Noel Barrett ,  Friedrich Dorner ,  Johann Eibl

    发明人： Wolfgang Mundt ,  Noel Barrett ,  Friedrich Dorner ,  Johann Eibl

    IPC分类号： A61K39/12 ,  C12N7/02 ,  C12N7/01

    CPC分类号： C12N7/00 ,  A61K39/12 ,  C12N2531/00 ,  C12N2770/24134 ,  C12N2770/24151

    摘要： The invention resides in a matrix, i.e. in a carrier material, with human or animal cells adherently bound thereto, the cells being infected with virus. It has shown that surface-dependent cells suitable for virus propagation remain adherently bound to a matrix even in the virus-infected state, continuously produce virus antigen over relatively long periods of time and deliver them into the culture medium. For producing TBE virus antigen by growing tick-borne encephalitis (TBE) virus in cell cultures, a surface-dependent permanent cell line, preferably the Vero cell line ATCC CCL 81, is inoculated with TBE virus, and the cells are kept bound to carriers in a non-lyric serum-free system while maintaining the cell growth, so as to maintain antigen formation, whereupon the antigen-containing medium is separated form the carrier-bound cells and, in a known manner, is processed to a galencially acceptable preparation by concentration, inactivation and purification.

    摘要翻译： 本发明涉及一种基质，即在载体材料中，与人或动物细胞贴壁结合，细胞被病毒感染。 已经表明，即使在病毒感染状态下，适用于病毒传播的表面依赖性细胞仍保持与基质结合，在相对长的时间内连续产生病毒抗原并将其输送到培养基中。 为了通过在细胞培养物中生长蜱传导性脑炎（TBE）病毒来生产TBE病毒抗原，表面依赖的永久性细胞系（优选Vero细胞系ATCC CCL 81）用TBE病毒接种，并且细胞保持与载体结合 在保持细胞生长的同时，在非抒情的无血清系统中，从而保持抗原形成，由此将含抗原的培养基从载体结合的细胞分离，并以已知的方式加工成半衰期可接受的制剂 通过浓缩，灭活和纯化。