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    Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (SPM) reading
    41.
    发明授权
    Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (SPM) reading 失效
    用于扫描探针显微镜（SPM）读数的纳米条形码的控制对准编码特定信息

    公开(公告)号：US07476786B2

    公开(公告)日：2009-01-13

    申请号：US10667004

    申请日：2003-09-19

    申请人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  A61K38/00 ,  C07K1/00

    CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

    摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by scanning probe microscopy (SPM), such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and scanning probe microscopy (SPM) analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.

    摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含与一个或多个纳米条形码连接的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米条形码可以是通过扫描探针显微镜（SPM）可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米粒子或量子点。 当探针为寡核苷酸时，与靶核酸杂交的相邻编码探针可在对准和扫描探针显微镜（SPM）分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

    Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
    43.
    发明授权
    Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading 有权
    用于扫描探针显微镜（SPM）读数的编码特定信息的纳米棒的受控比对

    公开(公告)号：US07361821B2

    公开(公告)日：2008-04-22

    申请号：US10251152

    申请日：2002-09-20

    申请人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

    摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.

    摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含连接至一个或多个纳米糖基的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米线可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米颗粒或量子点。 当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

    DETECTION OF DNA METHYLATION USING RAMAN SPECTROSCOPY
    47.
    发明申请
    DETECTION OF DNA METHYLATION USING RAMAN SPECTROSCOPY 审中-公开
    使用拉曼光谱法检测DNA甲基化

    公开(公告)号：US20080241828A1

    公开(公告)日：2008-10-02

    申请号：US11694427

    申请日：2007-03-30

    申请人： Kai Wu ,  Xing Su ,  Mineo Yamakawa

    发明人： Kai Wu ,  Xing Su ,  Mineo Yamakawa

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2523/125 ,  C12Q2565/628

    摘要： Epigenetic events such as DNA methylation play important roles in the regulation of gene expression. DNA methylation patterns have been found to differ between healthy and diseased tissue, such as healthy and cancerous tissue, thereby allowing DNA methylation to serve as a biomarker for disease states. Embodiments of the invention provide methods for detecting methylation patterns in DNA polymers. Methylation patterns are detected, in part, through the use of surface enhanced Raman spectroscopy (SERS). SERS provides a sensitive structure-based technique for chemical analysis.

    摘要翻译： 表观遗传事件如DNA甲基化在调控基因表达中起重要作用。 已经发现DNA甲基化模式在健康和患病组织如健康和癌组织之间不同，从而允许DNA甲基化作为疾病状态的生物标志物。 本发明的实施方案提供了检测DNA聚合物中甲基化模式的方法。 甲基化模式部分地通过使用表面增强拉曼光谱（SERS）来检测。 SERS提供了一种敏感的基于结构的化学分析技术。

    Methods to increase nucleotide signals by Raman scattering
    48.
    发明授权
    Methods to increase nucleotide signals by Raman scattering 有权
    通过拉曼散射增加核苷酸信号的方法

    公开(公告)号：US07238477B2

    公开(公告)日：2007-07-03

    申请号：US10660902

    申请日：2003-09-12

    申请人： Xing Su ,  Andrew A. Berlin ,  Selena Chan ,  Steven J. Kirch ,  Tac-Woong Koo ,  Gabi Neubauer ,  Valluri Rao ,  Narayanan Sundararajan ,  Mineo Yamakawa

    发明人： Xing Su ,  Andrew A. Berlin ,  Selena Chan ,  Steven J. Kirch ,  Tac-Woong Koo ,  Gabi Neubauer ,  Valluri Rao ,  Narayanan Sundararajan ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  G01N21/63

    CPC分类号： C12Q1/6869 ,  C12Q2565/632 ,  C12Q2563/155 ,  C12Q2521/319

    摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.

    摘要翻译： 本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。 在本发明的某些实施方案中，在掺入核酸之前，核苷酸与拉曼标记物共价连接。 在其它实施方案中，使用未标记的核酸。 核酸外切核酸处理导致通过拉曼光谱法检测的标记或未标记的核苷酸的释放。 在本发明的替代实施方案中，通过外切核酸酶处理从核酸释放的核苷酸与纳米颗粒共价交联，并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干反斯托克斯拉曼 光谱学（CARS）。 本发明的其它实施方案涉及用于核酸测序的装置。

    Methods to increase nucleotide signals by raman scattering
    50.
    发明申请
    Methods to increase nucleotide signals by raman scattering 审中-公开
    通过拉曼散射增加核苷酸信号的方法

    公开(公告)号：US20060166243A1

    公开(公告)日：2006-07-27

    申请号：US11329693

    申请日：2006-01-10

    申请人： Xing Su ,  Andrew Berlin ,  Selena Chan ,  Steven Kirch ,  Tac-Woong Koo ,  Gabi Neubauer ,  Valluri Rao ,  Narayanan Sundararajan ,  Mineo Yamakawa

    发明人： Xing Su ,  Andrew Berlin ,  Selena Chan ,  Steven Kirch ,  Tac-Woong Koo ,  Gabi Neubauer ,  Valluri Rao ,  Narayanan Sundararajan ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6869 ,  C12Q2565/632 ,  C12Q2563/155 ,  C12Q2521/319

    摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.

    摘要翻译： 本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。 在本发明的某些实施方案中，在掺入核酸之前，核苷酸与拉曼标记物共价连接。 在其它实施方案中，使用未标记的核酸。 核酸外切核酸处理导致通过拉曼光谱法检测的标记或未标记的核苷酸的释放。 在本发明的替代实施方案中，通过外切核酸酶处理从核酸释放的核苷酸与纳米颗粒共价交联，并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干反斯托克斯拉曼 光谱学（CARS）。 本发明的其它实施方案涉及用于核酸测序的装置。