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75 条记录 (耗时 0.053 秒)

    METHOD FOR PRODUCING L-FUCULOSE AND METHOD FOR PRODUCING L-FUCOSE
    42.
    发明申请
    METHOD FOR PRODUCING L-FUCULOSE AND METHOD FOR PRODUCING L-FUCOSE 失效
    生产L-纤维素的方法和生产L-纤维的方法

    公开(公告)号：US20070026504A1

    公开(公告)日：2007-02-01

    申请号：US11532618

    申请日：2006-09-18

    申请人： Shunichi Suzuki ,  Kunihiko Watanabe

    发明人： Shunichi Suzuki ,  Kunihiko Watanabe

    IPC分类号： C12P19/02 ,  C12N9/02 ,  C12N1/21

    CPC分类号： C12P19/02 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0036

    摘要： The present invention provides a method for producing L-fuculose and L-fucose which is suitable as an industrial method. L-Fuculose is synthesized from L-fucitol in the presence of a microorganism-derived protein having a dehydrogenase activity which results in production of L-fuculose from L-fucitol. The reaction system preferably contains NADH oxidase. L-Fuculose thus synthesized is then converted into L-fucose.

    摘要翻译： 本发明提供一种适用于工业方法的L-岩藻糖和L-岩藻糖的生产方法。 在具有脱氢酶活性的微生物衍生的蛋白质的存在下，从L-岩藻糖醇合成L-赖螺蛋白糖，其导致从L-岩藻糖醇生产L-赖螺酮糖。 反应体系优选含有NADH氧化酶。 然后将由此合成的L-福乐糖转化成L-岩藻糖。

    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid
    43.
    发明申请
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法和产生旋光氨基酸的方法

    公开(公告)号：US20060240532A1

    公开(公告)日：2006-10-26

    申请号：US11476572

    申请日：2006-06-29

    申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC分类号： C12P13/04 ,  C12P13/22 ,  C07H21/04 ,  C12N9/80 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

    摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。

    Mutant protein having the peptide-synthesizing activity
    45.
    发明申请
    Mutant protein having the peptide-synthesizing activity 审中-公开
    具有肽合成活性的突变蛋白

    公开(公告)号：US20060188976A1

    公开(公告)日：2006-08-24

    申请号：US11311467

    申请日：2005-12-20

    申请人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

    发明人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

    IPC分类号： C12N9/48 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C07K14/00 ,  C07K14/195 ,  C12N9/93

    摘要： The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant protein having the amino acid sequence containing one or more mutations of the above mutations 1 to 38 in the amino acid sequence described in SEQ ID NO:2; and (II) The mutant protein having the amino acid sequence containing one or more mutations selected from the group consisting of substitution, deletion, insertion, addition and inversion at positions other than one or more mutation positions of the above mutation 1 to 38 in the mutant protein described in the above (I), and having a peptide-synthesizing activity.

    摘要翻译： 本发明提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质的存在下，使胺组分和羧基组分反应合成肽：（I）具有含有一个或多个突变的氨基酸序列的突变蛋白 在SEQ ID NO：2中描述的氨基酸序列中的上述突变1至38; 和（II）具有在上述变异株1〜38中的一个以上突变位置以外的位置上具有选自取代，缺失，插入，添加和倒位的一个或多个突变的氨基酸序列的突变蛋白质 上述（I）所述的突变型蛋白质，具有肽合成活性。

    Xylitol dehydrogenase of acetic acid bacteria and gene thereof
    47.
    发明授权
    Xylitol dehydrogenase of acetic acid bacteria and gene thereof 失效
    乙酸细菌的木糖醇脱氢酶及其基因

    公开(公告)号：US06242228B1

    公开(公告)日：2001-06-05

    申请号：US09363189

    申请日：1999-07-29

    申请人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

    发明人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P718

    CPC分类号： C12N9/0006 ,  C12P7/18

    摘要： Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.

    摘要翻译： 通过使木糖醇脱氢酶或用编码木糖醇脱氢酶的DNA（其是以下（A）或（B）的蛋白质）作用于D-木酮糖并收集产生的木糖醇的DNA产生木糖醇：（A） 具有SEQ ID NO：4的氨基酸序列;（B）具有SEQ ID NO：4的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或倒位，并且具有木糖醇 脱氢酶活性。

    Semiconductor memory
    49.
    发明授权
    Semiconductor memory 失效
    半导体存储器

    公开(公告)号：US4622655A

    公开(公告)日：1986-11-11

    申请号：US607026

    申请日：1984-05-04

    申请人： Shunichi Suzuki

    发明人： Shunichi Suzuki

    IPC分类号： G11C11/409 ,  G11C11/4099 ,  G11C11/24

    CPC分类号： G11C11/4099

    摘要： A semiconductor memory has at least a pair of bit lines, a plurality of word lines crossing the pair of bit lines, a pair of dummy word lines crossing the pair of bit lines, memory cells arranged at intersections between the bit lines and the word lines, dummy cells arranged at intersections between any the bit lines and the dummy word lines, a sense amplifier connected to the pair of bit lines, and a means for equalizing the potentials of the pair of bit lines. Each of the memory cell has a transistor and a capacitor. Each dummy cell has the same construction as each memory cell. The pair of bit lines and dummy cell capacitors are electrically connected at a predetermined timing and are set at a third voltage, the corresponding dummy cell is disconnected from one of the bit line pair, to which a selected memory cell capacitor is connected. Subsequently, a voltage difference between voltages on the bit lines is detected.

    摘要翻译： 半导体存储器具有至少一对位线，与该对位线交叉的多条字线，与该对位线交叉的一对虚拟字线，位于位线和字线之间的交叉处的存储单元 布置在任何位线和虚拟字线之间的交叉处的虚拟单元，连接到所述一对位线的读出放大器以及用于均衡所述一对位线的电位的装置。 每个存储单元具有晶体管和电容器。 每个虚拟单元具有与每个存储单元相同的结构。 一对位线和虚设单元电容器以预定的定时电连接并被设置为第三电压，相应的虚设单元与连接所选存储单元电容器的位线对之一断开。 随后，检测位线上的电压之间的电压差。