公开(公告)号：US11788108B2

公开(公告)日：2023-10-17

申请号：US17355962

申请日：2021-06-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Harald Sobek ,  Michael Greif ,  Marco Thomann ,  Sebastian Malik

IPC: C12P21/00 ,  C12N9/10 ,  C12P19/44 ,  C07K16/00

CPC classification number: C12P21/005 ,  C07K16/00 ,  C12N9/1081 ,  C12P19/44 ,  C12Y204/99001 ,  C12Y301/03 ,  C07K2317/41

Abstract: The properties of certain glycosyltransferase variants having N-terminal truncation deletions or internal deletions are disclosed. Particularly, mutants that exhibit α-2,6-sialyltransferase enzymatic activity in the presence of CMP-activated sialic acid as co-substrate, and in the presence of a suitable acceptor site, are disclosed. A fundamental finding documented in the present disclosure is that enzymes are not only capable of catalyzing transfer of a sialidyl moiety but they are also capable of catalyzing hydrolytic cleavage of terminally bound sialic acid from a glycan.

公开(公告)号：US20230309870A1

公开(公告)日：2023-10-05

申请号：US18171469

申请日：2023-02-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Thomas

IPC: A61B5/1495 ,  A61B5/145

CPC classification number: A61B5/1495 ,  A61B5/14532

Abstract: An automated method of calibrating a sensor located in a flow-through sensor path and requiring at least one oxygenated calibration fluid for calibration comprises transporting a predefined amount of deoxygenated calibration fluid from a fluid supply into a fluidic line between a fluid-selection valve and the sensor path, transporting ambient air into the fluidic line before and after transporting the calibration fluid forming an isolated plug of calibration fluid between zones of ambient air, oscillating the calibration fluid plug back and forth within a predefined length of the fluidic line causing reciprocating fluid film tailing along the inner walls of the fluidic line, and facilitating oxygenation of the calibration fluid via oxygen uptake by the fluid film from the ambient air in the fluidic line, and transporting the oxygenated calibration fluid into the sensor path at a position where the sensor is located and calibrating the sensor.

公开(公告)号：US20230289688A1

公开(公告)日：2023-09-14

申请号：US18178595

申请日：2023-03-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hermann Hofstetter ,  Alejandro Morcillo Montejo

IPC: G06Q10/0631 ,  G06Q10/0639

CPC classification number: G06Q10/06316 ,  G06Q10/06398

Abstract: A computer-implemented method of providing user guidance for laboratory issue resolution within a laboratory system is presented. The method comprises monitoring activity by a user within a laboratory setting of the laboratory system by a user guidance system, detecting a laboratory issue within the laboratory system, retrieving by the user guidance system a preferred resolution to the detected laboratory issue and typical time to perform preferred solution from a database, notifying the user to the laboratory issue, detecting a non-preferred issue resolution for the laboratory issue being performed by the user, displaying the user guidance system to the user, wherein the user guidance system provides the retrieved preferred resolution for the laboratory issue to the user, resolving the laboratory issue by the user using the user guidance system, and removing or updating the display of the user guidance system following the resolution of the laboratory issue.

公开(公告)号：US11747356B2

公开(公告)日：2023-09-05

申请号：US17128268

申请日：2020-12-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christopher Bates ,  Julian Pfaff ,  Noelia Gonzalez Vila ,  Adrian Fernandez Vazquez ,  Kyle Benton ,  Attila Sapi

IPC: G01N35/04 ,  B01L9/06

CPC classification number: G01N35/04 ,  B01L9/06 ,  G01N2035/0418 ,  G01N2035/0477

Abstract: A support element for a modular transport plane with a plurality of transport module units each comprising a driving surface assembly is presented. The support element has an upper support surface supporting the driving surface assemblies of at least two neighboring transport module units. The upper support surface has driving surface assembly interfaces engaged with complementary interfaces of the supported driving surface assemblies. The support element comprises a lower part having a mounting structure and an upper part having the upper support surface. The upper part connects lengthwise to the lower part via a connection structure. The connection structure restrains a relative movement between the lower part and the upper part in the longitudinal direction of the support element and allows a limited relative movement between the lower part and the upper part in a plane perpendicular to the longitudinal direction of the support element.

公开(公告)号：US11733253B2

公开(公告)日：2023-08-22

申请号：US17119307

申请日：2020-12-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Marco Maetzler

IPC: G01N35/00

CPC classification number: G01N35/0092 ,  G01N35/00871

Abstract: A method to optimize analyzer use in a laboratory having a plurality of analyzers based on laboratory workload is presented. The method comprises determining current laboratory workload, calculating workload capability of the plurality of analyzers minus one analyzer if the current laboratory workload is below a threshold criteria and if there are two or more analyzers in the plurality of analyzers, masking one of the plurality of analyzers if the current workload is met by the plurality of analyzers minus one analyzer, proceeding with current workload, and repeating the above steps until the current laboratory workload has been completed.

公开(公告)号：US20230258680A1

公开(公告)日：2023-08-17

申请号：US18137254

申请日：2023-04-20

Applicant: Ventana Medical Systems, Inc. ,  Roche Diagnostics Operations, Inc.

Inventor： Andreas Drechsler ,  Oliver Gutmann ,  Melis Hazar ,  Reto Huesser ,  Raymond T. Kozikowski, III ,  Daniel Mueller ,  Chris Steinert

IPC: G01N35/10 ,  G01N1/31

CPC classification number: G01N35/1016 ,  G01N1/312 ,  G01N35/1002 ,  G01N35/1011 ,  G01N35/1009 ,  G01N2001/317 ,  G01N2035/1041

Abstract: A system and method for treatment of biological samples is disclosed. In some embodiments, an automated biological sample staining system (100), comprising at least one microfluidic reagent applicator (118); at least one bulk fluid applicator (116); at least one fluid aspirator; at least one sample substrate holder; at least one relative motion system; and a control system (102) that is programmed to execute at least one staining protocol on a sample mounted on a substrate that is held in the at least one sample substrate holder.

公开(公告)号：US11724246B2

公开(公告)日：2023-08-15

申请号：US16599260

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/26 ,  B01J20/28 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

CPC classification number: B01J20/28009 ,  B01J20/267 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/3234 ,  B01J20/3272 ,  B01J20/3282 ,  B01J20/3293 ,  B03C1/01 ,  G01N33/54326 ,  B03C2201/18 ,  B03C2201/26

Abstract: A method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M) is disclosed. Further, particles obtained or obtainable by this method as well as to the use of these particles are disclosed. In a further aspect, a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte is disclosed.

公开(公告)号：US11719714B2

公开(公告)日：2023-08-08

申请号：US16645583

申请日：2019-03-19

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION ,  ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： Sayaka Sarwar ,  Masaharu Nishida ,  Yoko Inoue ,  Masafumi Shimada ,  Kenichi Yagi ,  Naohiko Fukaya ,  Andrew McCaughey

IPC: G01N35/10 ,  G01N35/00

CPC classification number: G01N35/1002 ,  G01N35/00603 ,  G01N35/1016 ,  G01N2035/1018

Abstract: The automatic analyzer includes: a liquid dispensing mechanism that performs suction of a liquid; a pressure sensor that measures a change of an internal pressure of a probe provided at the liquid dispensing mechanism; a determination section that determines whether the suction of the liquid by the probe is normal suction or air suction abnormality; an analysis section; a storage section that stores a cumulative number of times of air suction abnormality per liquid and an allowable cumulative number for the cumulative number of times of air suction abnormality; and a controller that exercises operation control over the liquid dispensing mechanism, the determination section, and the analysis section. The controller exercises control such that the cumulative number of times of air suction abnormality is updated and the updated cumulative number is stored in the storage section even if the air suction abnormality occurs non-consecutively.

公开(公告)号：US11709172B2

公开(公告)日：2023-07-25

申请号：US17120874

申请日：2020-12-14

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Marco Maetzler ,  Christopher Schofield ,  Ole Lambaek ,  Moritz von Hopffgarten

IPC: G01N35/00 ,  G01N35/10 ,  B01L3/02 ,  G16H10/40

CPC classification number: G01N35/00871 ,  B01L3/021 ,  G01N35/0095 ,  G01N35/00722 ,  G01N35/1016 ,  G16H10/40 ,  B01L2200/14 ,  G01N2035/009 ,  G01N2035/00881

Abstract: A method for operating a laboratory system comprising instruments for processing samples and a control unit connected by a communication network is presented. The method comprises receiving and identifying a biological sample and retrieving an order list from a database. The list comprises a plurality of targets defining one or more processing steps to be carried out on the biological sample by one or more of the laboratory instruments. The method also comprises selecting a workflow strategy and retrieving workflow acceptance criterion corresponding to the workflow strategy. The control unit determines a sample workflow for processing the sample based on the workflow strategy and determines whether the sample workflow satisfies the workflow acceptance criterion. If the sample workflow does not satisfy the workflow acceptance criterion, workflow strategy and the workflow acceptance criterion is refined and the sample workflow is determined again until it satisfies the workflow acceptance criterion.

公开(公告)号：US20230221316A1

公开(公告)日：2023-07-13

申请号：US17999222

申请日：2021-05-19

Applicant: Roche Diagnostics Operations, Inc. ,  Roche Diagnostics Hematology, Inc.

Inventor： Anna Maria NEUBAUER-PERCHUC ,  Jan-Gerrit HOOGENDIJK ,  Nicole GRETHER ,  David J. ZAHNISER ,  Katherine K. MUI ,  Michael TACKE ,  Nora TORRES-NAGEL

IPC: G01N33/569 ,  G01N1/30 ,  G01N35/00 ,  G01N35/10

CPC classification number: G01N33/56972 ,  G01N1/30 ,  G01N35/00029 ,  G01N35/1002 ,  G01N2001/302 ,  G01N2333/705

Abstract: The present disclosure relates generally to methods and systems for detecting, characterizing biomarker expression and morphological analysis in cell samples. The methods allow for the use of automated platforms to stain cells for molecular biomarkers and Romanowsky-type staining for cell morphology analysis. Cells that are prepared according to the disclosed methods can also be used in the diagnosis of certain conditions.