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    REDUCING SPATIAL OCCUPANCY OF MOLECULES IN A SAMPLE THROUGH SAMPLE CROSSLINKING
    44.
    发明公开
    REDUCING SPATIAL OCCUPANCY OF MOLECULES IN A SAMPLE THROUGH SAMPLE CROSSLINKING 审中-公开

    公开(公告)号：US20240132938A1

    公开(公告)日：2024-04-25

    申请号：US18478392

    申请日：2023-09-29

    申请人： 10x Genomics, Inc.

    发明人： Malte KÜHNEMUND

    IPC分类号： C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876

    CPC分类号： C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876

    摘要： The present disclosure in some aspects relates to methods and compositions for confining molecules generated in a biological sample. In particular examples, rolling circle amplification (RCA) products are generated in a fixed biological sample which has been crosslinked prior to RCA, such that the three-dimensional crosslinked matrix generated prior to RCA confines the RCA products, and the RCA products are smaller than they would otherwise be in a fixed biological sample which has not been crosslinked prior to RCA. Confining the RCA products by generating them in a pre-crosslinked matrix in the biological sample may result in compaction of the RCA products and facilitate subsequent in situ analysis. The sample crosslinking can be performed after probe hybridization to nucleic acid molecules in the sample and before RCA.

    Nucleic acid sequencing using self-luminescence
    49.
    发明授权
    Nucleic acid sequencing using self-luminescence 有权

    公开(公告)号：US11952624B1

    公开(公告)日：2024-04-09

    申请号：US18088097

    申请日：2022-12-23

    申请人： GeneSense Technology Inc.

    发明人： Mei Yan

    IPC分类号： C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q1/6876

    CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6876

    摘要： Methods and kits for sequencing a nucleic acid molecule are provided, which include utilizing four different compounds that are respectively derivatives of nucleotides A, (T/U), C and G, wherein the hydroxyl at the 3′-position of sugar of each of the four compounds is protected by a reversible protecting group. Each of the four compounds comprises a first linker, a second linker, and a terminal molecular label binding to or reactive to a receptor in a detectable group comprising a luminescence-activating molecule and the receptor, the luminescence-activating molecule capable of causing emission of fluorescence in the presence of a suitable substrate. The second linkers in the four compounds are different, and can be cleaved at different conditions. After incorporating one of the four compounds into a growing chain using the target nucleic acid molecule as a template, a series of reactions is performed to cleave the second linker and the first linker, and a series of detections are performed for fluorescent signals, thereby determining the identity of the nucleotide of the incorporated compound.