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    METHOD OF DIAGNOSING IRRITABLE BOWEL SYNDROME AND OTHER DISORDERS CAUSED BY SMALL INTESTINAL BACTERIAL OVERGROWTH BY DETECTING THE PRESENCE OF ANTI-SACCHAROMYCES CERIVISIAE ANTIBODIES (ASCA) IN HUMAN SERUM
    42.
    发明授权
    METHOD OF DIAGNOSING IRRITABLE BOWEL SYNDROME AND OTHER DISORDERS CAUSED BY SMALL INTESTINAL BACTERIAL OVERGROWTH BY DETECTING THE PRESENCE OF ANTI-SACCHAROMYCES CERIVISIAE ANTIBODIES (ASCA) IN HUMAN SERUM 有权
    通过检测人类血清中抗真菌抗体（ASCA）的存在，诊断由小肠细菌引起的有用的贝类综合症和其他疾病的方法

    公开(公告)号：US06562629B1

    公开(公告)日：2003-05-13

    申请号：US09374143

    申请日：1999-08-11

    申请人： Henry C. Lin ,  Mark Pimentel

    发明人： Henry C. Lin ,  Mark Pimentel

    IPC分类号： G01N33564

    CPC分类号： G01N33/6854 ,  G01N2333/395

    摘要： Disclosed is a method of diagnosing small intestinal bacterial overgrowth (SIBO), irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, attention deficit/hyperactivity disorder (ADHD), or an autoimmune disease by sampling serum from a human subject having a suspected diagnosis of any of these conditions and analyzing the serum for the presence of ASCA, which corroborates the suspected diagnosis. A method of determining a predisposition for developing Crohn's, in a human subject who does not present a set of symptoms characteristic of the disease and who has small intestinal bacterial overgrowth, involves sampling serum from the subject and analyzing the serum for the presence of ASCA. The presence of ASCA in the serum indicates a predisposition for developing Crohn's disease. Also disclosed is a kit for diagnosing and treating small intestinal bacterial overgrowth, irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, attention deficit/hyperactivity disorder, or an autoimmune disease, such as multiple sclerosis or systemic lupus erythematosus. The kit is useful to improve symptoms, including hyperalgesia related to SIBO and disorders caused by SIBO.

    摘要翻译： 公开了通过从具有疑似诊断的人类受试者取样血清来诊断小肠细菌过度生长（SIBO），肠易激综合征，纤维肌痛，慢性疲劳综合征，抑郁症，注意力缺陷/多动症（ADHD）或自身免疫性疾病的方法 的任何这些条件，并分析血清中ASCA的存在，这证实了疑似诊断。 确定在不具有该疾病特征并且具有小肠细菌过度生长的一组症状的人类受试者中发展克罗恩病的易感性的方法包括从受试者取样血清并分析血清中存在ASCA。 血清中ASCA的存在表明发生克罗恩病的倾向。 还公开了用于诊断和治疗小肠细菌过度生长，肠易激综合征，纤维肌痛，慢性疲劳综合征，抑郁症，注意力缺陷/多动症或自身免疫性疾病如多发性硬化症或系统性红斑狼疮的试剂盒。 该试剂盒可用于改善症状，包括与SIBO相关的痛觉过敏和由SIBO引起的疾病。

    Fungal IPC synthase assay
    43.
    发明授权
    Fungal IPC synthase assay 失效
    真菌IPC合酶测定

    公开(公告)号：US6022684A

    公开(公告)日：2000-02-08

    申请号：US944594

    申请日：1997-10-06

    申请人： Jeffrey Radding ,  Robert C. Dickson ,  Robert L. Lester

    发明人： Jeffrey Radding ,  Robert C. Dickson ,  Robert L. Lester

    IPC分类号： C12Q1/18 ,  C12Q1/48 ,  G01N33/50 ,  C12Q1/00 ,  C12Q1/02

    CPC分类号： C12Q1/18 ,  C12Q1/48 ,  G01N2333/395

    摘要： The presently-disclosed IPC synthase-inhibitor assays comprise the steps of: (1) expression of the IPC1 gene in a cell; (2) introducing labeled starting substrates for ceramide conversion as well as potential inhibitor(s) of such conversion to the expressed gene product in an environment which allows time and conditions for conversion, and (3) identifying those potential inhibitors which actually inhibit conversion. The present invention also provides methods to determine the ability of a test compound to inhibit fungal growth, comprising the steps of (1) presenting active inositolphosophotidylceramide synthase in a manner such that synthesis of inositolphosphotidylceramide can occur; (2) introducing ceramide and phosphotylinositol, said ceramide or phosphatidylinositol carrying label for identification; (3) subjecting said active inositolphosophotidylceramide synthase, ceramide and phosphotylinositol to ordinary conditions necessary for ceramide conversion to phosphoinositol ceramide; and (4) identifying those test compounds which inhibit ceramide conversion to phosphoinositol ceramide.

    摘要翻译： 目前公开的IPC合酶抑制剂测定法包括以下步骤：（1）在细胞中表达IPC1基因; （2）在允许转化时间和条件的环境中引入用于神经酰胺转化的标记的起始底物以及这种转化的表达基因产物的潜在抑制剂，和（3）鉴定那些实际上抑制转化的潜在抑制剂。 本发明还提供了确定测试化合物抑制真菌生长的能力的方法，包括以下步骤：（1）以可以发生肌醇磷脂酰肌醇酰胺的合成的方式呈递活性肌醇磷脂酰肌醇酰胺合酶; （2）引入神经酰胺和磷酸肌醇，所述神经酰胺或磷脂酰肌醇携带标签进行鉴定; （3）使所述活性肌醇六磷脂酰胆碱合成酶，神经酰胺和磷酰肌醇进行神经酰胺转化为磷酸肌醇神经酰胺所必需的常规条件; 和（4）鉴定抑制神经酰胺转化为磷酸肌醇神经酰胺的那些试验化合物。

    UREOHYDROLASES AS DOMINANT SELECTABLE MARKERS IN YEAST
    45.
    发明申请
    UREOHYDROLASES AS DOMINANT SELECTABLE MARKERS IN YEAST 审中-公开

    公开(公告)号：US20170298368A1

    公开(公告)日：2017-10-19

    申请号：US15301599

    申请日：2015-04-13

    申请人： HEINEKEN SUPPLY CHAIN B.V.

    发明人： Jean-Marc Georges Daran ,  Jacobus Thomas Pronk ,  Gabriele Romagnoli

    IPC分类号： C12N15/81 ,  C12N9/78 ,  C12Q1/04

    CPC分类号： C12N15/81 ,  C12N9/78 ,  C12Q1/04 ,  C12Y305/03007 ,  G01N2333/395 ,  G01N2333/978

    摘要： The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a microorganism having an altered genome. The invention further relates to a set of constructs, comprising a first construct comprising a recognition site for an endonuclease, a first region of homology with a target gene of a microorganism, and a first part of a nucleotide sequence encoding the selection marker, and a second construct comprising a second part of the nucleotide sequence encoding the selection marker, a second region of homology with the target gene of the microorganism, and a copy of the endonuclease recognition site. The invention further relates to methods for altering a target gene in a microorganism, to methods for producing a microorganism, and to microorganisms that are produced by the methods of the invention.

    Prion-like form of CPEB and related compositions and methods
    47.
    发明授权
    Prion-like form of CPEB and related compositions and methods 有权
    朊病毒样形式的CPEB及相关成分和方法

    公开(公告)号：US08951734B2

    公开(公告)日：2015-02-10

    申请号：US10578203

    申请日：2004-11-05

    申请人： Kausik Si ,  Eric Kandel

    发明人： Kausik Si ,  Eric Kandel

    IPC分类号： C12Q1/00 ,  C12Q1/02 ,  C12Q1/68 ,  C07K14/435 ,  C07K14/47

    CPC分类号： C12Q1/6897 ,  C07K14/43504 ,  C07K14/4702 ,  G01N2333/395

    摘要： The present invention provides methods for determining whether an agent facilitates the conversion of a cytoplasmic polyadenylation element binding (CPEB) protein from its non-prion form to its prion form. This invention also provides methods for determining the presence and amount of the prion form of CPEB protein in a cell. This invention also provides methods for facilitating the conversion of a non-prion CPEB protein to its prion form. This invention also provides an isolated prion form cytoplasmic polyadenylation element binding (CPEB) protein and compositions comprising a therapeutically effective amount of an agent that facilitates the conversion of CPEB protein from its non-prion form to its prion form, and methods of making such compositions.

    摘要翻译： 本发明提供了用于确定试剂是否促进细胞质多腺苷酸化元件结合（CPEB）蛋白从其非朊病毒形式转化为其朊病毒形式的方法。 本发明还提供了确定细胞中CPEB蛋白质的朊病毒形式的存在和量的方法。 本发明还提供了促进非朊病毒CPEB蛋白转化成其朊病毒形式的方法。 本发明还提供分离的朊病毒形式的细胞质多腺苷酸化元件结合（CPEB）蛋白质和包含治疗有效量的促进CPEB蛋白从其非朊病毒形式转化为其朊病毒形式的药剂的组合物，以及制备这种组合物的方法 。

    YEAST-BASED METHODS OF IDENTIFYING NUCLEIC ACIDS AND COMPOUNDS FOR TREATING NEURODEGENERATIVE DISEASES
    48.
    发明申请
    YEAST-BASED METHODS OF IDENTIFYING NUCLEIC ACIDS AND COMPOUNDS FOR TREATING NEURODEGENERATIVE DISEASES 有权
    基于YEAST的识别核酸和化合物治疗神经营养因子疾病的方法

    公开(公告)号：US20110250607A1

    公开(公告)日：2011-10-13

    申请号：US13063865

    申请日：2009-11-20

    申请人： Stevan Marcus ,  Galina Gulis ,  P.Connor Johnson

    发明人： Stevan Marcus ,  Galina Gulis ,  P.Connor Johnson

    IPC分类号： C12Q1/68 ,  C12Q1/02

    CPC分类号： C12Q1/04 ,  G01N2333/395 ,  G01N2800/2835

    摘要： The disclosure encompasses methods for the screening of small molecules or nucleic acids that may reverse the inhibition of growth of the unicellular yeast Schizosaccharomyces pombe by rotenone. The use of a yeast as the screening target allows for the high-throughput screening of small molecule and nucleic acid libraries for candidates that may then be screened in animal models as therapeutic agents for the treatment of neurodegenerative diseases. The plant-derived isoflavonoid, rotenone, while only moderately inhibitory to S. pombe cell growth on complex rich medium, is highly inhibitory to growth on synthetic minimal medium. S. pombe cells carrying a deletion in the gene pmk1 are hypersensitive to rotenone. S. pombe, therefore, provides a model for elucidating complex 1-independent targets of rotenone, and can serve as a screening tool for identifying compounds or oligonucleotides potentially able to reverse the effects of rotenone or Parkinson's disease in animal or human subjects.

    摘要翻译： 本公开包括用于筛选小分子或核酸的方法，所述小分子或核酸可以通过鱼藤酮来抑制单细胞酵母粟酒裂殖酵母的生长的抑制。 使用酵母作为筛选靶标允许对可能在动物模型中筛选的候选物的小分子和核酸文库的高通量筛选作为治疗神经变性疾病的治疗剂。 植物来源的异黄酮，鱼藤酮，而对富含复合物的培养基上的S.pombe细胞生长只有适度抑制，对合成基本培养基的生长具有高度的抑制作用。 在基因pmk1中携带缺失的粟酒酵母细胞对鱼藤酮具有过敏性。 因此，S.pombe提供了用于阐明鱼藤酮的复合物1-非依赖性靶标的模型，并且可以用作鉴定在动物或人类受试者中潜在地能够逆转鱼藤酮或帕金森病的作用的化合物或寡核苷酸的筛选工具。