公开(公告)号：US4762617A

公开(公告)日：1988-08-09

申请号：US3667

申请日：1987-01-15

申请人： Fred J. Stevens

发明人： Fred J. Stevens

IPC分类号： B01D15/08 ,  G01N30/38 ,  G01N30/86 ,  G01N30/89

CPC分类号： G01N30/38 ,  B01D15/34 ,  G01N30/8658 ,  G01N30/89

摘要： A low pressure, microcomputer controlled system employing high performance liquid chromatography (HPLC) allows for precise analysis of the interaction of two reversibly associating macromolecules such as proteins. Since a macromolecular complex migrates faster than its components during size-exclusion chromatography, the difference between the elution profile of a mixture of two macromolecules and the summation of the elution profiles of the two components provides a quantifiable indication of the degree of molecular interaction. This delta profile is used to qualitatively reveal the presence or absence of significant interaction or to rank the relative degree of interaction in comparing samples and, in combination with a computer simulation, is further used to quantify the magnitude of the interaction in an arrangement wherein a microcomputer is coupled to analytical instrumentation in a novel manner.

摘要翻译： 使用高效液相色谱（HPLC）的低压微电脑控制系统允许精确分析两个可逆缔合的大分子如蛋白质的相互作用。 由于大分子复合物在尺寸排阻层析期间比其组分迁移速度更快，两个大分子的混合物的洗脱曲线与两个组分的洗脱曲线的总和之间的差异提供了分子相互作用程度的可量化指示。 这种增量分布图用于定性地揭示存在或不存在显着的相互作用，或者在比较样本中相对交互程度的排序，并结合计算机模拟进一步用于量化布置中的相互作用的大小，其中a 微机以新颖的方式耦合到分析仪器。

公开(公告)号：US20190195840A1

公开(公告)日：2019-06-27

申请号：US15952367

申请日：2018-04-13

申请人： TAIHO PHARMACEUTICAL CO., LTD.

发明人： Yoshinori TOKIMITSU

IPC分类号： B01J20/281 ,  C07H19/06 ,  G01N30/89

CPC分类号： G01N30/48 ,  C07H19/06 ,  G01N30/89 ,  G01N2030/027 ,  G01N2030/486

摘要： This invention provides a method that is capable of detecting a trifluridine-related substance and a tipiracil-related substance contained in a sample containing trifluridine or a salt thereof and tipiracil or a salt thereof using the same procedure. The method is for detecting a trifluridine-related substance or a tipiracil-related substance or both, the method comprising the step of subjecting a sample containing trifluridine or a salt thereof and tipiracil or a salt thereof to high-performance liquid chromatography using a mobile phase composed of an organic phase and an aqueous phase.

公开(公告)号：US20160274019A1

公开(公告)日：2016-09-22

申请号：US15032220

申请日：2014-09-03

申请人： SARTORIUS STEDIM BIOTECH GMBH

发明人： Rebecca Petersen ,  Axel Thiefes ,  Katrin Köhler ,  Wolfgang Demmer ,  Sven Dankenbrink

IPC分类号： G01N15/08

CPC分类号： G01N15/082 ,  G01N30/89 ,  G01N2015/084 ,  G01N2030/889

摘要： The present invention relates to a method for determining the functionality and integrity of depth filter sheets and depth filter sheet systems comprising inorganic layered double hydroxide for contaminant removal in biotechnological processes.

摘要翻译： 本发明涉及一种用于确定深度过滤片和深度过滤片系统的功能性和完整性的方法，该系统包括用于生物技术过程中污染物去除的无机层状双氢氧化物。

公开(公告)号：US20130192340A1

公开(公告)日：2013-08-01

申请号：US13809586

申请日：2011-07-15

申请人： Baizhan Liu ,  Wenjuan Wang ,  Saijing Zheng ,  Da Wu

发明人： Baizhan Liu ,  Wenjuan Wang ,  Saijing Zheng ,  Da Wu

IPC分类号： G01N30/02

CPC分类号： G01N30/02 ,  G01N30/461 ,  G01N2030/025 ,  G01N30/89

摘要： The present invention provides a gas chromatography—inverse gas chromatography combined analysis device, which includes a gas chromatography column and an inverse gas chromatography column, an input end of the gas chromatography column is connected to a sample feeder, an output end of the gas chromatography column is connected to an input end of the inverse gas chromatography column, the output end of the gas chromatography column is further connected to a first detector, the input end of the inverse gas chromatography column is further connected to a carrier gas tube, an output end of the inverse gas chromatography column is connected to a second detector, and the first detector and the second detector are both connected to a signal collector. The present invention not only can investigate adsorption performance of a tested solid adsorption material with respect to a single probe, but also can investigate adsorption performance of different solid adsorption materials with respect to different constituents in a combined probe at the same time, thereby improving the development efficiency of the inverse gas chromatography technologies.

摘要翻译： 本发明提供一种气相色谱 - 反相气相色谱组合分析装置，其包括气相色谱柱和反相气相色谱柱，气相色谱柱的输入端连接到样品进料器，气相色谱法的输出端 柱连接到反相气相色谱柱的输入端，气相色谱柱的输出端进一步连接到第一检测器，反相气相色谱柱的输入端进一步连接到载气管，输出端 反相气相色谱柱的末端连接到第二检测器，第一检测器和第二检测器都连接到信号收集器。 本发明不仅可以研究测试固体吸附材料相对于单个探针的吸附性能，而且可以同时研究不同固体吸附材料在组合探针中的不同成分的吸附性能，从而改善 逆气相色谱技术的开发效率。

公开(公告)号：US20070068385A1

公开(公告)日：2007-03-29

申请号：US11559760

申请日：2006-11-14

申请人： Andrew Tipler ,  Neil Plant

发明人： Andrew Tipler ,  Neil Plant

IPC分类号： B01D53/02

CPC分类号： G01N30/89 ,  G01N15/0826 ,  G01N30/32 ,  G01N30/60 ,  G01N2030/324 ,  G01N2030/524 ,  G01N2030/626 ,  G01N2030/525

摘要： Disclosed are systems and methods that include providing a vessel having an adsorbent disposed therein, the vessel having an inlet and an outlet for communicating a carrier gas through the vessel; determining a differential pressure between the inlet and the outlet for a known flow rate at the outlet; and, determining a geometric measure for the vessel based on a ratio of the known flow rate and the differential pressure. In some embodiments, the methods and systems can include a viscosity in the ratio, determining a flow rate at ambient pressure using a factor based on a ratio of a gas pressure at the output and ambient pressure, and/or, determining a flow rate at ambient temperature using a factor based on a ratio of a temperature at the output and ambient temperature.

摘要翻译： 公开了包括提供其中设置有吸附剂的容器的系统和方法，所述容器具有用于使载气通过容器连通的入口和出口; 确定出口处的已知流速的入口和出口之间的压差; 并且基于已知流量和压差的比率确定容器的几何度量。 在一些实施方案中，方法和系统可以包括该比例的粘度，使用基于输出处的气体压力和环境压力的比率的因子确定环境压力下的流速，和/或确定 使用基于输出温度和环境温度的比率的因子的环境温度。

公开(公告)号：US20050210957A1

公开(公告)日：2005-09-29

申请号：US11072371

申请日：2005-03-04

申请人： Andrew Tipler ,  Neil Plant

发明人： Andrew Tipler ,  Neil Plant

IPC分类号： B01D46/46 ,  G01B13/00 ,  G01B13/08 ,  G01N15/08 ,  G01N30/08 ,  G01N30/32 ,  G01N30/52 ,  G01N30/54 ,  G01N30/60 ,  G01N30/62 ,  G01N30/86 ,  G01N30/89

CPC分类号： G01N30/89 ,  G01N15/0826 ,  G01N30/32 ,  G01N30/60 ,  G01N2030/324 ,  G01N2030/524 ,  G01N2030/626 ,  G01N2030/525

摘要： Disclosed are systems and methods that include providing a vessel having an adsorbent disposed therein, the vessel having an inlet and an outlet for communicating a carrier gas through the vessel; determining a differential pressure between the inlet and the outlet for a known flow rate at the outlet; and, determining a geometric measure for the vessel based on a ratio of the known flow rate and the differential pressure. In some embodiments, the methods and systems can include a viscosity in the ratio, determining a flow rate at ambient pressure using a factor based on a ratio of a gas pressure at the output and ambient pressure, and/or, determining a flow rate at ambient temperature using a factor based on a ratio of a temperature at the output and ambient temperature.

公开(公告)号：US20030006192A1

公开(公告)日：2003-01-09

申请号：US10161705

申请日：2002-06-05

发明人： Irving Wainer ,  Yanxiao Zhang ,  Farideh Beigi ,  Ruin Moaddel

IPC分类号： B01D015/08

CPC分类号： B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3251 ,  B01J20/3274 ,  B01J2220/54 ,  B01J2220/58 ,  G01N30/02 ,  G01N30/48 ,  G01N30/7233 ,  G01N30/89 ,  G01N33/543 ,  G01N33/566 ,  G01N2030/527 ,  G01N2333/4719

摘要： This invention provides an immobilized G-protein coupled receptor (GPCR) on a support in a liquid chromatography system. The method of the invention provides means of evaluating the attachment of an agent to a GPCR comprising the steps of: (a) immobilizing a GPCR on an artificial membrane support in a column, (b) exposing the support with the GPCR to test the agent at varying concentrations in a liquid chromatography system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test material from the column. Using this method, it is possible to evaluate the interaction of the test agent with the GPCR. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.

摘要翻译： 本发明在液相色谱系统的载体上提供了固定的G蛋白偶联受体（GPCR）。 本发明的方法提供了评估试剂与GPCR的连接的方法，包括以下步骤：（a）将GPCR固定在柱上的人造膜载体上，（b）用GPCR暴露载体以测试试剂 在液相色谱系统中以不同的浓度，（c）从柱中洗脱测试试剂，和（d）从柱中评估测试材料的洗脱曲线。 使用这种方法，可以评估测试代理与GPCR的相互作用。 洗脱后，可以通过将洗脱液通过其它测试装置如质谱仪来直接确定分子结构。

公开(公告)号：US20020168773A1

公开(公告)日：2002-11-14

申请号：US09924021

申请日：2001-08-07

发明人： Tomoaki Ito ,  Masashi Fujita

IPC分类号： G01N030/02

CPC分类号： B01J47/14 ,  B01J41/04 ,  G01N30/89 ,  G01N2030/889 ,  Y10T436/204998

摘要： The performance of an anion exchange resin is evaluated on-line by measuring inorganic carbonic acid concentrations at the inlet and at the outlet of an ion exchange resin vessel (such as a demineralizer vessel) of an ion exchange apparatus such as a condensate water demineralization system. Moreover, an MTC (Mass Transfer Coefficient) with respect to the inorganic carbonic acid is calculated and the dynamic performance and/or degree of degradation of the anion exchange resin are evaluated. The degree of degradation of the anion exchange resin is evaluated from the MTC and the replacement timing, lifetime, and throughput capacity for the anion exchange resin are judged.

摘要翻译： 阴离子交换树脂的性能通过测量诸如冷凝水脱矿质系统之类的离子交换装置的离子交换树脂容器（如软化器容器）的入口处和出口处的无机碳酸浓度在线评价 。 此外，计算相对于无机碳酸的MTC（质量传递系数），并评价阴离子交换树脂的动态性能和/或降解程度。 从MTC评价阴离子交换树脂的降解程度，判断阴离子交换树脂的更换时间，寿命和通过量。

公开(公告)号：US06387974B1

公开(公告)日：2002-05-14

申请号：US09923935

申请日：2001-08-07

申请人： Karl Chaplin Deissler ,  Marlin Kenneth Kinzey ,  John Joseph Maikner ,  Robert E. Rosen

发明人： Karl Chaplin Deissler ,  Marlin Kenneth Kinzey ,  John Joseph Maikner ,  Robert E. Rosen

IPC分类号： C08F3600

CPC分类号： B01J20/285 ,  B01D15/20 ,  B01D15/325 ,  B01D15/327 ,  B01D15/34 ,  B01J20/26 ,  B01J20/261 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28057 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/28095 ,  B01J20/287 ,  B01J20/30 ,  B01J20/3064 ,  B01J2220/52 ,  B01J2220/54 ,  G01N30/482 ,  G01N30/89 ,  Y10S530/815

摘要： Macroporous polymers having selected porosity and permeability characteristics that provide rigid polymer matrices suitable for use in medium and high pressure reversed phase liquid chromatography (RPC) are disclosed. A method for preparing the polymers using selected mixed porogens in selected proportions relative to the monomer phase is also disclosed. The polymers are especially useful as stationary phases in large scale chromatography columns without developing increased pressures during prolonged use, while maintaining good chromatographic performance for targeted biomolecules, such as insulin.

摘要翻译： 公开了具有选择的孔隙率和渗透率特性的大孔聚合物，其提供适用于中高压反相液相色谱（RPC）的刚性聚合物基质。 还公开了使用相对于单体相的选定比例的选择的混合致孔剂制备聚合物的方法。 聚合物在大规模色谱柱中作为固定相是特别有用的，在长时间使用时不会增加压力，同时保持目标生物分子如胰岛素的良好色谱性能。

公开(公告)号：US20020023878A1

公开(公告)日：2002-02-28

申请号：US09864595

申请日：2001-05-23

发明人： Nathan Collins ,  Jeffrey Wheatley

IPC分类号： B01D015/08

CPC分类号： B01D15/24 ,  B01D15/10 ,  G01N30/34 ,  G01N30/6095 ,  G01N30/8658 ,  G01N30/89 ,  G01N2030/8804 ,  G01N2030/8886 ,  Y10T436/12

摘要： A component of a chemical mixture is isolated via a high-throughput purification process. An analytical retention time and corresponding analytical chromatographic parameters are determined for the component. Based on the analytical retention time and the corresponding analytical chromatographic parameters, preparative chromatographic parameters are determined to isolate the component at an accelerated retention time using a preparative column. The chemical mixture is eluted through the preparative column using the preparative chromatographic parameters, and the component is isolated at the accelerated retention time.

摘要翻译： 化学混合物的组分通过高通量纯化方法分离。 确定组分的分析保留时间和相应的分析色谱参数。 基于分析保留时间和相应的分析色谱参数，确定制备色谱参数以使用制备柱在加速保留时间分离组分。 使用制备色谱参数将化学混合物通过制备柱洗脱，并且在加速保留时间分离组分。