公开(公告)号：US20020063208A1

公开(公告)日：2002-05-30

申请号：US09994575

申请日：2001-11-27

Applicant: SURROMED, INC.

Inventor： Curtis Hastings

IPC: H01J049/00

CPC classification number: H01J49/0036 ,  G01N30/72 ,  G01N30/8624 ,  G01N30/8641 ,  H01J49/0431

Abstract: High-intensity, spiked noise is reduced in chromatography-mass spectrometry data by applying a nonlinear filter such as a moving median filter to the data. The filter is applied to individual mass chromatograms, plots of ion abundance versus retention time for each detected mass-to-charge ratio, and the filtered chromatograms are combined to form a filtered total ion current chromatogram. Standard linear filters are not effective for reducing noise in liquid chromatography-mass spectrometry (LC-MS) data because they assume a normal distribution of noise. LC-MS noise, however, is not normally distributed.

Abstract translation: 通过对数据应用诸如移动中值滤波器的非线性滤波器，在色谱 - 质谱数据中降低了高强度，加标噪声。 将过滤器应用于单个质量色谱图，每种检测质量 - 电荷比的离子丰度曲线与保留时间曲线，并将滤出的色谱图合并形成过滤的总离子电流色谱图。 标准线性滤波器对于降低液相色谱 - 质谱（LC-MS）数据中的噪声无效，因为它们假设噪声的正态分布。 然而，LC-MS噪声不是正态分布的。

公开(公告)号：US20020036263A1

公开(公告)日：2002-03-28

申请号：US09814800

申请日：2001-03-23

Applicant: ANELVA CORPORATION

Inventor： Yoshiro Shiokawa ,  Megumi Nakamura ,  Toshihiro Fujii

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/147 ,  H01J49/107 ,  H01J49/145

Abstract: A mass spectrometry apparatus is provided with a mass spectrometry mechanism for analyzing the mass of ionized detected gas. The mass spectrometry apparatus is further provided with two ion sources, that is, a first ion source for attaching positive charge metal ions to cause ionization, and a second ion source for causing electrons to impact to cause ionization. Based on the configuration, it becomes possible to simultaneously or separately measure the molecular weight and analyze the molecular structure of the detected gas with a high sensitivity. The second ion source is positioned between the first ion source and the mass spectrometry mechanism and the detected gas is introduced into the first ion source. According to the above mass spectrometry apparatus, it is possible to measure the accurate molecular weight of the detected gas with a sufficient sensitivity and to simultaneously analyze the molecular structure with a sufficient sensitivity.

Abstract translation: 质谱装置设置有用于分析电离检测气体的质量的质谱分析机构。 该质谱装置还具有两个离子源，即用于附加正电荷金属离子以引起电离的第一离子源和用于引起电子撞击以引起电离的第二离子源。 基于该结构，可以以高灵敏度同时或分别测量分子量并分析检测到的气体的分子结构。 第二离子源位于第一离子源和质谱分析机构之间，检测到的气体被引入第一离子源。 根据上述质谱装置，可以以足够的灵敏度测定检测气体的精确分子量，并以足够的灵敏度同时分析分子结构。

公开(公告)号：US20020036261A1

公开(公告)日：2002-03-28

申请号：US09905536

申请日：2001-07-13

Applicant: Epion Corporation

Inventor： Jerald P. Dykstra

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J37/304 ,  H01J2237/0812

Abstract: The invention provides methods and apparatus for measuring the distribution of cluster ion sizes in a gas cluster ion beam (GCIB) and for determining the mass distribution and mass flow of cluster ions in a GCIB processing system without necessitating the rejection of a portion of the beam through magnetic or electrostatic mass analysis. The invention uses time-of-flight measurement to estimate or monitor cluster ion size distribution either before or during processing of a workpiece. The measured information is displayed and incorporated in automated control of a GCIB processing system.

Abstract translation: 本发明提供了用于测量气体簇离子束（GCIB）中的团簇离子尺寸分布并用于确定GCIB处理系统中的团簇离子的质量分布和质量流量的方法和装置，而不需要排除一部分束 通过磁性或静电质量分析。 本发明使用飞行时间测量在工件处理之前或处理期间估计或监测簇离子尺寸分布。 测量的信息被显示并结合在GCIB处理系统的自动控制中。

公开(公告)号：US20020027196A1

公开(公告)日：2002-03-07

申请号：US09824211

申请日：2001-04-03

Inventor： Yoshiro Shiokawa

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/4215

Abstract: A Q-pole type mass spectrometer which can be used under a high-pressure atmosphere of more than 0.1 Pa is provided. The said Q-pole type mass spectrometer can analyze mass of gas molecule continuously, and can separate mass properly even if ion is injected at high speed in order to reduce an influence of an end electric field near an end face (fringing) of the Q-pole. In this invention, it is noticed that the motion of ion to be measured in the diameter direction is independent of the motion of ion in the axial direction within the Q-pole region of the Q-pole type mass spectrometer. In the Q-pole type mass spectrometer installed in a reduced pressure atmosphere, the motion of ion to be measured in the axial direction advancing from an ion source toward a collector is controlled within the Q-pole region so as to separate mass of the ion to be measured by Coulomb force generated by a quadrupole high-frequency electric field in the diameter direction.

Abstract translation: 提供可以在高于0.1Pa的高压气氛下使用的Q极型质谱仪。 所述Q极型质谱仪可以连续分析气体分子的质量，即使高速注入离子，也可以分离质量，以减少Q值端面（边缘）附近末端电场的影响 -极。 在本发明中，注意到直径方向的测定离子的运动与Q极型质谱仪的Q极区域内的轴向的离子运动无关。 在安装在减压气氛中的Q极型质谱仪中，从离子源朝向集电体的轴向测定的离子的运动被控制在Q极区域内，以分离出离子的质量 通过在直径方向上由四极高频电场产生的库仑力来测量。

公开(公告)号：US20020020812A1

公开(公告)日：2002-02-21

申请号：US09866855

申请日：2001-05-29

Inventor： Marvin L. Vestal ,  Peter Juhasz

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/164 ,  H01J49/403

Abstract: A time-of-flight mass spectrometer for measuring the mass-to-charge ratio of a sample molecule is described. The spectrometer provides independent control of the electric field experienced by the sample before and during ion extraction. Methods of mass spectrometry utilizing the principles of this invention reduce matrix background, induce fast fragmentation, and control the transfer of energy prior to ion extraction.

Abstract translation: 描述了用于测量样品分子的质荷比的飞行时间质谱仪。 离子提取之前和期间，光谱仪可以独立控制样品经历的电场。 使用本发明原理的质谱法降低了基质背景，诱导快速碎裂，并控制离子提取前的能量转移。

公开(公告)号：US20020011563A1

公开(公告)日：2002-01-31

申请号：US09927666

申请日：2001-08-10

Applicant: Isis Pharmaceuticals, Inc.

Inventor： Richard Griffey ,  Steven Hofstadler

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/0059 ,  Y10T436/143333 ,  Y10T436/24

Abstract: The present invention discloses novel methods and apparatuses for mass spectrometry. In the methods and apparatuses of the invention, ions are accumulated in an ion reservoir and dissociated with coherent radiation prior to mass analysis. These methods and apparatuses are amenable to mass spectrometric analysis of biomolecules and are particularly useful for the sequencing of oligonucleotides, peptides and oligosaccharides.

Abstract translation: 本发明公开了用于质谱的新颖的方法和装置。 在本发明的方法和装置中，在质量分析之前，离子积聚在离子储存器中并与相干辐射分离。 这些方法和装置适于生物分子的质谱分析，并且对寡核苷酸，肽和寡糖的测序特别有用。

公开(公告)号：US20020003209A1

公开(公告)日：2002-01-10

申请号：US09754736

申请日：2001-01-04

Inventor： Troy D. Wood ,  E. Peter Maziarz III ,  Sarah A. Lorenz ,  Thomas P. White

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/0018 ,  G01N30/7266 ,  H01J49/167

Abstract: The present invention relates to a nanospray emitter including an emitter body which includes a fluid inlet, an outlet orifice, and a passage communicating between the fluid inlet and outlet orifice; and an electrically conductive polymer coating on at least a portion of the emitter body. Also disclosed are a nano-electrospray device including the nanospray emitter of the present invention, a method for making a nanospray emitter of the present invention, a method of forming a nanospray using the nanospray emitter of the present invention, and a method of analyzing a solution using the nanospray emitter of the present invention.

Abstract translation: 本发明涉及纳喷射发射器，其包括发射体，其包括流体入口，出口孔和在流体入口和出口孔之间连通的通道; 以及在发射体的至少一部分上的导电聚合物涂层。 还公开了包括本发明的纳米喷雾发射体的纳米电喷雾装置，制备本发明的纳米喷雾发射体的方法，使用本发明的纳米喷雾发射体形成纳米喷雾的方法，以及分析 使用本发明的纳米喷雾发射体。

公开(公告)号：US20020000517A1

公开(公告)日：2002-01-03

申请号：US09764698

申请日：2001-01-18

Inventor： Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser ,  Xian Huang

IPC: H01J049/00 ,  B01D059/44

CPC classification number: G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles for the same fluid for analysis. This invention dramatically increases the sensitivity of microchip electrospray devices compared to prior disclosed systems and methods. The silicon chip having the packed microchannels disclosed herein finds application in coupling with mass spectrometry for sample analysis. Also disclosed is a separation block having multiple through-substrate channels filled with a separation material such as polymer monolith which can be stacked in multiple blocks for sequential two-dimensional chromatographic separation and integrated with the electrospray device.

Abstract translation: 公开了一种具有分离材料的微细硅芯片，例如在其微通道中原位制备的多孔聚合物整料。 聚合物整料是液体可渗透的，用作液相色谱的微柱，其通过在微通道中含有乙烯基单体和溶剂（致孔剂）的混合物的原位自由基聚合制备。 公开了一种方法和系统，以从一个或多个喷嘴产生一个或多个电喷雾羽流，其提供与质量分析仪测量的离子强度，其大小与为流体中包含的分析形成的电喷雾羽流的数量成比例。 多个电喷雾装置可以以小型化的独立电喷雾装置阵列的形式使用，用于从用于分析的相同流体的多个喷嘴产生多个电喷雾羽流。 与先前公开的系统和方法相比，本发明显着提高了微芯片电喷雾装置的灵敏度。 具有本文公开的包装微通道的硅芯片可以与质谱联用进行样品分析。 还公开了一种分离块，其具有填充有诸如聚合物整料的分离材料的多个通过基底通道，其可以堆叠在多个块中用于顺序的二维色谱分离并与电喷雾装置集成。

公开(公告)号：US20010054686A1

公开(公告)日：2001-12-27

申请号：US09811904

申请日：2001-03-19

Inventor： Richard P. Torti ,  Matthew C. Gwinn ,  Jerald P. Dykstra

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/10 ,  H01J27/026 ,  H01J49/0422 ,  H01J2237/0812 ,  H01J2237/24485

Abstract: A detector apparatus and its use for cluster ion beam diagnostics are described. The detector has a Faraday cup with a conductance path to a gas pressure detector and a conductance to the detector exit. The detector acquires ion current, which is a measure of the ion beam flux, and also acquires mass flux, through a pressure measurement. The pressure measurement responds to the mass of dissociated gas clusters and is combined with information about instantaneous ion current to estimate mean gas cluster ion size ({overscore (N)}i).

Abstract translation: 描述了一种检测器装置及其用于群集离子束诊断的用途。 检测器具有法拉第杯，其具有通向气体压力检测器的电导路径和对检测器出口的电导。 检测器获取离子电流，其是离子束通量的量度，并且还通过压力测量获得质量通量。 压力测量响应于解离的气体团簇的质量，并与关于瞬时离子电流的信息相结合以估计平均气体团簇离子大小（{overscore（N）} i）。

公开(公告)号：US20010054684A1

公开(公告)日：2001-12-27

申请号：US09087535

申请日：1998-05-29

Inventor： MELVIN A. PARK

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/406 ,  H01J49/0068

Abstract: The present invention relates generally to time-of-flight mass spectrometers and discloses an improved method and apparatus for analyzing ions using a time-of-flight mass spectrometer. More specifically, a means and method are described for the use of tandem time-of-flight mass spectrometry in conjunction with surface induced dissociation and pulsed ion extraction for fragmentation and analysis of selected sample ions. The concept essential to SID with PIE is that the kinetic energy of product and scattered ions can be correlated with their position at some time T after the collision event, and by using this relationship, one can reduce the distribution of either the kinetic energy of the ions or the arrival times of the ions at some position in the spectrometer. Ions are produced from sample material in an ion source and pulsed into the SID-PIE instrument. The packet of ions thus produced may or may not be mass analyzed before striking the SID surface. Ions of interest are accelerated to a kinetic energy appropriate to the desired fragmentation and then allowed to strike the SID surface.

Abstract translation: 本发明一般涉及飞行时间质谱仪，并且公开了一种使用飞行时间质谱仪分析离子的改进方法和装置。 更具体地，描述了使用串联飞行时间质谱联用表面诱导的离解和脉冲离子提取以用于选择的样品离子的碎裂和分析的方法和方法。 SID与PIE的关键概念是产物和散射离子的动能可以与碰撞事件后的某一时间T的位置相关联，并且通过使用这种关系，可以减少其中的动能 离子或离子在光谱仪中的某个位置的到达时间。 离子源由离子源中的样品材料制成并脉冲进入SID-PIE仪器。 在撞击SID表面之前，这样产生的离子包可以被分析或者不被质量分析。 感兴趣的离子被加速到适合于所需碎裂的动能，然后允许撞击SID表面。