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公开(公告)号:US20120083426A1
公开(公告)日:2012-04-05
申请号:US13188031
申请日:2011-07-21
申请人: Claus Lindvald JOHANSEN , Soren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
发明人: Claus Lindvald JOHANSEN , Soren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
IPC分类号: C40B30/08 , G01N33/566 , C12N9/88 , C09K3/00 , G01N33/573 , C07K14/00 , C12Q1/26 , C12Q1/02 , C07K1/14 , C12N9/04
摘要: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
摘要翻译: 描述了一种用于释放可溶性或膜相关胞内细胞内蛋白质(POI)的方法,包括以下步骤:提供包含可溶性或膜相关细胞内POI的细胞; 使细胞与膜提取组合物接触; 并且使得POI在足以特异性释放POI并且以可溶形式的条件下从细胞释放。
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公开(公告)号:US20110212090A1
公开(公告)日:2011-09-01
申请号:US13055321
申请日:2009-07-22
申请人: Henrik Pedersen , Liselotte Brix
发明人: Henrik Pedersen , Liselotte Brix
IPC分类号: A61K39/395 , A61P35/00 , A61K31/704 , A61P31/04 , A61P31/06 , A61K31/496 , A61K39/00 , A61K39/02
摘要: A method for the repair of a unit, by specific diagnosis of the undesired state, and its appropriate repair, using said specific diagnosis as a means to repair in an appropriate way said unit. The diagnosis and repair processes may involve chemical, physical, or mechanical means. The units being diagnosed and repaired include live matter (e.g. human beings, animals, plants) as well as non-live matter (e.g. buildings, electronic equipment, polymer materials).
摘要翻译: 通过特定诊断不期望的状态及其适当的修复,使用所述特定诊断作为以适当方式修复所述单元的手段来修复单元的方法。 诊断和修复过程可能涉及化学,物理或机械手段。 被诊断和修复的单位包括活物(例如人类,动物,植物)以及非活体物质(例如建筑物,电子设备,聚合物材料)。
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公开(公告)号:US07919275B2
公开(公告)日:2011-04-05
申请号:US12719648
申请日:2010-03-08
CPC分类号: C12Q1/6895 , C12N9/14
摘要: An isolated mutant host cell produced by disrupting or deleting, in a parent cell, a nucleic acid sequence encoding an oxaloacetate hydrolase, or a promoter thereof, which results in the mutant cell producing less of the oxaloacetate hydrolase than the parent cell is disclosed. The disclosure also relates to recombinant methods for producing the products such as polypeptides.
摘要翻译: 公开了一种分离的突变宿主细胞,其通过在母体细胞中破坏或缺失编码草酰乙酸水解酶的核酸序列或其启动子,导致突变体细胞产生比母细胞少的草酰乙酸酯水解酶。 本公开还涉及用于生产诸如多肽的产物的重组方法。
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54.
公开(公告)号:US20100168390A1
公开(公告)日:2010-07-01
申请号:US12644554
申请日:2009-12-22
申请人: Liselotte Brix , Henrik Pedersen , Tina Jakobsen , Jorgen Scholler , Jesper Lohse , Katja Brunstedt , Kivin Jacobsen
发明人: Liselotte Brix , Henrik Pedersen , Tina Jakobsen , Jorgen Scholler , Jesper Lohse , Katja Brunstedt , Kivin Jacobsen
IPC分类号: C07K14/74
CPC分类号: C07K14/70539 , A61K38/00 , A61K47/61 , A61K47/6425 , A61K47/665 , B82Y5/00 , Y02A50/55 , Y02A50/57 , Y02A50/58
摘要: The present invention describes novel methods to generate MHC multimers and methods to improve existing and new MHC multimers. The invention also describes improved methods for the use of MHC multimers in analysis of T-cells in samples including diagnostic and prognostic methods. Furthermore the use of MHC multimers in therapy are described, e.g. anti-tumour and anti-virus therapy, including isolation of antigen specific T-cells capable of inactivation or elimination of undesirable targeT-cells or isolation of specific T-cells capable of regulation of other immune cells.
摘要翻译: 本发明描述了生成MHC多聚体的新方法和改进现有的和新的MHC多聚体的方法。 本发明还描述了在样品中T细胞分析中使用MHC多聚体的改进方法,包括诊断和预后方法。 此外,MHC多聚体在治疗中的使用描述如下。 抗肿瘤和抗病毒治疗,包括分离能够灭活或消除不需要的targeT细胞的抗原特异性T细胞或分离能够调节其他免疫细胞的特异性T细胞。
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55.
公开(公告)号:US20090264300A1
公开(公告)日:2009-10-22
申请号:US12095778
申请日:2006-12-01
申请人: Thomas Franch , Mikkel Dybro Lundorf , Soren Nyboe Jacobsen , Eva Kampmann Olsen , Anne Lee Andersen , Anette Holtmann , Anders Holm Hansen , Anders Malling Sorensen , Anne Goldbech , Daen de Leon , Ditte Kievsmose Kaldor , Frank Abildgaard Slok , Gitte Nystrup Husemoen , Johannes Dolberg , Kim Birkebaek Jensen , Lene Pedersen , Mads Norregaard-Madsen , Michael Anders Godskesen , Sanne Schroder Glad , Sore Neve , Thomas Thisted , Tine Titilola Akinleminu Kronborg , Christian Klarner Sams , Jakob Felding , Per-Ola Freskgard , Alex Haahr Gouliaev , Henrik Pedersen
发明人: Thomas Franch , Mikkel Dybro Lundorf , Soren Nyboe Jacobsen , Eva Kampmann Olsen , Anne Lee Andersen , Anette Holtmann , Anders Holm Hansen , Anders Malling Sorensen , Anne Goldbech , Daen de Leon , Ditte Kievsmose Kaldor , Frank Abildgaard Slok , Gitte Nystrup Husemoen , Johannes Dolberg , Kim Birkebaek Jensen , Lene Pedersen , Mads Norregaard-Madsen , Michael Anders Godskesen , Sanne Schroder Glad , Sore Neve , Thomas Thisted , Tine Titilola Akinleminu Kronborg , Christian Klarner Sams , Jakob Felding , Per-Ola Freskgard , Alex Haahr Gouliaev , Henrik Pedersen
CPC分类号: C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要: Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
摘要翻译: 公开了一种获得包含与单链标识符寡核苷酸连接的分子的双功能复合物的方法,其中包含化学反应位点的新生双功能复合物和用于酶标记添加的引发位点是a)在化学反应位点与 一种或多种反应物,和b)在起始位点用一种或多种标记反应物的标签进行酶促反应。
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公开(公告)号:US20060234231A1
公开(公告)日:2006-10-19
申请号:US10518056
申请日:2003-06-20
申请人: Per-Ola Freskgard , Henrik Pedersen
发明人: Per-Ola Freskgard , Henrik Pedersen
CPC分类号: C07D405/04 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C12N15/1068 , C12P19/34 , C40B40/00
摘要: Disclosed is a microarray comprising a plurality of single stranded nucleic acid probes immobilized in discrete areas of a solid support, said probes being hybridised to a library of complexes, wherein each complex comprises an encoded molecule and a template which codes for said molecule, said template comprising a number of codons which codes for chemical entities which upon reaction form a reaction product which at least partly form part of the encoded molecule.
摘要翻译: 公开了一种微阵列,其包含固定在固体支持物的离散区域中的多个单链核酸探针,所述探针与复合物文库杂交,其中每个复合物包含编码分子和编码所述分子的模板,所述模板 包括编码化学实体的多个密码子,其在反应时形成至少部分形成编码分子的一部分的反应产物。
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公开(公告)号:US20050202502A1
公开(公告)日:2005-09-15
申请号:US11122506
申请日:2005-05-04
申请人: Carsten Hjort , Henrik Pedersen
发明人: Carsten Hjort , Henrik Pedersen
CPC分类号: C12Q1/6895 , C12N9/14
摘要: The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
摘要翻译: 本发明涉及编码具有草酰乙酸水解酶活性的多肽的分离的核酸序列。 本发明还涉及包含核酸序列的核酸构建体,载体和宿主细胞以及用于产生多肽的重组方法。
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58.
公开(公告)号:US20050176948A1
公开(公告)日:2005-08-11
申请号:US10507936
申请日:2003-03-14
申请人: Alex Gouliaev , Henrik Pedersen , Thomas Thisted , Mikkel Lundorf , Christian Sams , Thomas Franch , Gitte Husemoen , Justin Ho
发明人: Alex Gouliaev , Henrik Pedersen , Thomas Thisted , Mikkel Lundorf , Christian Sams , Thomas Franch , Gitte Husemoen , Justin Ho
IPC分类号: C07B61/00 , C07D405/04 , C07F9/6558 , C07F9/6561 , C07H21/00 , C07H21/02 , C07H23/00 , C12N15/10 , C12P19/34
CPC分类号: C07D405/04 , C07B2200/11 , C07F9/65586 , C07F9/65616 , C07H21/00 , C07H21/02 , C07H23/00 , C12N15/1068 , C12P19/34 , C40B40/00
摘要: A building block having the dual capabilities of transferring the genetic information e.g. by recognising an encoding element and transferring a functional entity to a recipient reactive group is diclosed. The building block can be designed with an adjustable transferability taking into account the components of the building block. The building block may be used in the generation of a single complex or libraries of different complexes, wherein the complex comprises an encoded molecule linked to an encoding element. Libraries of complexes are useful in the quest for pharmaceutically active compounds.
摘要翻译: 具有转移遗传信息的双重能力的构建块,例如, 通过识别编码元素并将功能实体传送到接收者反应组被关闭。 构建块可以设计成具有可调节的可转移性,同时考虑到构件的部件。 构建单元可以用于生成单个复合体或不同复合物的文库,其中复合物包含与编码元件连接的编码分子。 复合物库可用于寻求药物活性化合物。
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公开(公告)号:US06544765B1
公开(公告)日:2003-04-08
申请号:US09501612
申请日:2000-02-10
IPC分类号: C12N914
CPC分类号: C12Q1/6895 , C12N9/14
摘要: The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
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公开(公告)号:US06352988B2
公开(公告)日:2002-03-05
申请号:US09814037
申请日:2001-03-21
申请人: Jens Kristian Perregaard , Benny Bang-Andersen , Henrik Pedersen , Ivan Mikkelsen , Robert Dancer
发明人: Jens Kristian Perregaard , Benny Bang-Andersen , Henrik Pedersen , Ivan Mikkelsen , Robert Dancer
IPC分类号: A61K31496
CPC分类号: C07D401/04 , C07D209/08 , C07D209/14 , C07D401/14 , C07D491/04 , G11C5/025 , G11C7/1048
摘要: The present invention relates to substituted indane or dihydroindole compounds of Formula (I) wherein A is an indole. These compounds have high affinity for D4 receptors.
摘要翻译: 本发明涉及式(I)的取代的茚满或二氢吲哚化合物,其中A是吲哚。 这些化合物对D4受体具有高亲和力。
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