公开(公告)号：US20120258549A1

公开(公告)日：2012-10-11

申请号：US13433619

申请日：2012-03-29

申请人： Meng Lu ,  Chun Ge ,  Brian T. Cunningham ,  Stephen Schulz

发明人： Meng Lu ,  Chun Ge ,  Brian T. Cunningham ,  Stephen Schulz

IPC分类号： G01N21/75 ,  G01N21/64

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N2021/6421 ,  H01S3/1055 ,  H01S5/141 ,  H01S5/142

摘要： A label-free biosensor detection arrangement incorporating an external cavity laser (ECL) includes a tunable lasing element (e.g. an antireflection coated laser diode or semiconductor optical amplifier) and a narrow bandwidth resonant reflectance filter as the wavelength-selective element for the tunable lasing element. A sample is deposited on the surface of the resonant reflectance filter containing a biological material. The wavelength emitted by the external cavity laser is continuously tunable by binding interactions between the biological material and the resonant reflectance filter or adsorption of the biological material present in the sample on resonant reflectance filter. The narrow bandwidth resonance reflectance filter can take the form of photonic crystal (PC), a Bragg stack, or a Brag fiber reflection filter.

摘要翻译： 包含外腔激光器（ECL）的无标签生物传感器检测装置包括可调谐激光元件（例如抗反射涂层激光二极管或半导体光放大器）和窄带谐振反射滤光器，作为可调谐激光元件的波长选择元件 。 将样品沉积在含有生物材料的共振反射滤光片的表面上。 外腔激光器发出的波长可以通过生物材料和谐振反射滤光片之间的结合相互作用或者在样品中存在于共振反射滤光片上的生物材料的吸附来连续调节。 窄带谐振反射滤波器可以采用光子晶体（PC），布拉格堆叠或布拉格光纤反射滤光片的形式。

公开(公告)号：US20120007000A1

公开(公告)日：2012-01-12

申请号：US13135246

申请日：2011-06-28

申请人： Meng Lu ,  Stephen C. Schulz ,  Brian T. Cunningham ,  Anusha Pokhriyal

发明人： Meng Lu ,  Stephen C. Schulz ,  Brian T. Cunningham ,  Anusha Pokhriyal

IPC分类号： G01N21/64

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/7743

摘要： A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, which can be fabricated uniformly from plastic materials over a ˜3×5 in2 surface area by nanoreplica molding, features a 1-D periodic grating structure which utilizes two distinct resonant modes to enhance electric field stimulation of a first dye excited by a first laser (e.g., λ=632.8 nm laser exciting cyanine-5) and a second dye excited by a second laser (e.g., λ=532 nm laser exciting cyanine-3). The first and second lasers could be replaced by a single variable wavelength (tunable) laser. Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle θ. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in a given wavelength range (e.g., the range 532 nm

摘要翻译： 已经证明了表现出多个荧光团的共振增强的光子晶体衬底。 可以通过纳米尺寸成型在〜3×5 in2表面积上由塑料材料均匀地制造的器件具有1-D周期性光栅结构，其利用两种不同的谐振模式来增强第一染料激发的电场刺激 第一激光（例如，λ= 632.8nm激光花青-5）和由第二激光器激发的第二染料（例如，λ= 532nm激光花青-3）。 第一和第二激光器可以由单个可变波长（可调谐）激光器代替。 获得激光激发与光子晶体表面的共振耦合，每个波长处于不同的入射角。 通过选择适当的入射角，光子晶体能够通过给定波长范围（例如，532nm <λ<660nm）的激发波长放大任何荧光染料的输出。 该装置可用于在单个成像区域内利用多种荧光染料的生物测定法，例如基因表达微阵列。

公开(公告)号：US07863052B2

公开(公告)日：2011-01-04

申请号：US11490556

申请日：2006-07-20

申请人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

发明人： Stephen C. Schulz ,  Brian T. Cunningham ,  Lance G. Laing ,  Peter Y. Li ,  Brant Binder ,  Gangadhar Jogikalmath ,  Alex Borsody

IPC分类号： G01N33/551

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/7743 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/143333

摘要： A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label-free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.

公开(公告)号：US20100221847A1

公开(公告)日：2010-09-02

申请号：US12800082

申请日：2010-05-06

申请人： Brian T. Cunningham ,  Charles Choi

发明人： Brian T. Cunningham ,  Charles Choi

IPC分类号： G01N33/557

CPC分类号： G01N21/4133 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0487 ,  B82Y20/00 ,  G01N21/253 ,  G01N21/7743 ,  G02B2006/1213

摘要： Photonic crystal (PC) sensors, and sensor arrays and sensing systems incorporating PC sensors are described which have integrated fluid containment and/or fluid handling structures. The PC sensors are further integrated into a sample handling device such as a microwell plate. Sensors and sensing systems of the present disclosure are capable of high throughput sensing of analytes in fluid samples, bulk refractive index detection, and label-free detection of a range of molecules, including biomolecules and therapeutic candidates. The present disclosure also provides a commercially attractive fabrication platform for making photonic crystal sensors and systems wherein an integrated fluid containment structure and a photonic crystal structure are fabricated in a single molding or imprinting processing step amendable to high throughput processing.

摘要翻译： 描述了具有集成流体容纳和/或流体处理结构的光子晶体（PC）传感器以及包含PC传感器的传感器阵列和感测系统。 PC传感器进一步集成到诸如微孔板之类的样品处理装置中。 本公开的传感器和感测系统能够高流量感测流体样品中的分析物，体积折射率检测和一系列分子（包括生物分子和治疗候选物）的无标记检测。 本公开还提供了用于制造光子晶体传感器和系统的商业上有吸引力的制造平台，其中集成流体容纳结构和光子晶体结构在可修改为高通量处理的单个模制或压印处理步骤中制造。

公开(公告)号：US07756365B2

公开(公告)日：2010-07-13

申请号：US11822303

申请日：2007-07-02

申请人： Brian T. Cunningham ,  Nikhil Ganesh ,  Ian D. Block

发明人： Brian T. Cunningham ,  Nikhil Ganesh ,  Ian D. Block

IPC分类号： G02B6/00

CPC分类号： G01N21/7743 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B82Y20/00 ,  G01N21/253 ,  G01N21/33 ,  G01N33/54373 ,  G01N33/551 ,  G01N33/552 ,  G02B1/005

摘要： Biosensors are disclosed based on one- or two-dimensional photonic-crystal reflectance filters operating at near-ultraviolet wavelengths. The biosensors feature a tightly confined resonant electric field at the surface of this biosensor and provide a high surface-sensitivity to bulk-sensitivity ratio, and therefore enables enhanced detection resolution for biomolecules adsorbed on the biosensor surface. These new biosensors can be fabricated in mass by replica molding. They are especially well suited for applications requiring the detection of small molecules or ultra-low analyte concentrations.

摘要翻译： 基于在近紫外波长下操作的一维或二维光子晶体反射滤光片来公开生物传感器。 生物传感器在该生物传感器的表面具有紧密限制的共振电场，并提供对体敏感比的高表面灵敏度，因此能够增强吸附在生物传感器表面上的生物分子的检测分辨率。 这些新的生物传感器可以通过复制成型制成。 它们特别适用于需要检测小分子或超低分析物浓度的应用。

公开(公告)号：US20100085566A1

公开(公告)日：2010-04-08

申请号：US12586516

申请日：2009-09-22

申请人： Brian T. Cunningham

发明人： Brian T. Cunningham

IPC分类号： G01J3/44 ,  B05D5/12

CPC分类号： G01J3/44 ,  G01N21/658 ,  G02B1/005

摘要： Highly sensitive Surface Enhanced Raman Spectroscopy (SERS) sensors are described in the form of a optical resonator and a metal nanostructure deposited on surface of the optical resonator. In one embodiment the optical resonator is in the form of a photonic crystal, but other optical resonators are contemplated. Examples are described in which the resonant near-fields of a large-area replica molded photonic crystal efficiently couples light from a laser to dielectric-metal “post-cap” nanostructures deposited on the photonic crystal surface by a glancing angle evaporation technique, achieving a high SERS enhancement factor. Other constructions are also contemplated a metal nanostructure formed on a dielectric support deposited on the photonic crystal, including a metallic film deposited over close-packed surface of nanospheres, arrays of metallic nanotriangles, metallic nanorods, metallic nanohelices, arrays of metallic nanospheres, and roughened metal surfaces.

摘要翻译： 高灵敏度表面增强拉曼光谱（SERS）传感器以沉积在光学谐振器表面上的光学谐振器和金属纳米结构的形式进行描述。 在一个实施例中，光学谐振器是光子晶体的形式，但是可以考虑其它光学谐振器。 描述了大面积复制光子晶体的共振近场效应地将光从激光耦合到通过扫掠角蒸发技术沉积在光子晶体表面上的电介质金属“后盖”纳米结构，实现了 高SERS增强因子。 还考虑了其​​它结构，其形成在沉积在光子晶体上的电介质载体上，包括沉积在纳米球的紧密堆积表面上的金属膜，金属纳米管阵列，金属纳米棒，金属纳米螺旋，金属纳米球阵列和粗糙化 金属表面。

公开(公告)号：US07531786B2

公开(公告)日：2009-05-12

申请号：US11983108

申请日：2007-11-06

申请人： Brian T. Cunningham ,  Charles Choi

发明人： Brian T. Cunningham ,  Charles Choi

IPC分类号： H01L31/00

CPC分类号： B01L3/5085 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0654 ,  B82Y20/00 ,  G01N21/253 ,  G02B2006/1213

摘要： Photonic crystal (PC) sensors, and sensor arrays and sensing systems incorporating PC sensors are described which have integrated fluid containment and/or fluid handling structures. Sensors and sensing systems of the present disclosure are capable of high throughput sensing of analytes in fluid samples, bulk refractive index detection, and label-free detection of a range of molecules, including biomolecules and therapeutic candidates. The present disclosure also provides a commercially attractive fabrication platform for making photonic crystal sensors and systems wherein an integrated fluid containment structure and a photonic crystal structure are fabricated in a single molding or imprinting processing step amendable to high throughput processing.

摘要翻译： 描述了具有集成流体容纳和/或流体处理结构的光子晶体（PC）传感器以及包含PC传感器的传感器阵列和感测系统。 本公开的传感器和感测系统能够高流量感测流体样品中的分析物，体积折射率检测和一系列分子（包括生物分子和治疗候选物）的无标记检测。 本公开还提供了用于制造光子晶体传感器和系统的商业上有吸引力的制造平台，其中集成流体容纳结构和光子晶体结构在可修改为高通量处理的单个模制或压印处理步骤中制造。

公开(公告)号：US07497992B2

公开(公告)日：2009-03-03

申请号：US10434015

申请日：2003-05-08

申请人： Brian T. Cunningham ,  Peter Y. Li ,  Constance Chang-Hasnain ,  Carlos Mateus

发明人： Brian T. Cunningham ,  Peter Y. Li ,  Constance Chang-Hasnain ,  Carlos Mateus

IPC分类号： C12M1/34 ,  C12M3/00 ,  G01N33/53 ,  G01N21/00

CPC分类号： G01N21/45 ,  G01N21/39 ,  G01N21/75 ,  G01N21/7743 ,  G01N33/54373

摘要： An apparatus and method for detection of peak wavelength values of colorimetric resonant optical biosensors using tunable filters and tunable lasers is provided. Biomolecular interactions may be detected on a biosensor by directing collimated white light towards a surface of the biosensor. Molecular binding on the surface of the biosensor is indicated by a shift in the peak wavelength value of reflected or transmitted light from the biosensor, while an increase in the wavelength corresponds to an increase in molecular absorption. A tunable laser light source may generate the collimated white light and a tunable filter may receive the reflected or transmitted light and pass the light to a photodiode sensor. The photodiode sensor then quantifies an amount of the light reflected or transmitted through the tunable filter as a function of the tuning voltage of the tunable filter.

摘要翻译： 提供了使用可调滤波器和可调谐激光器检测比色共振光学生物传感器的峰值波长值的装置和方法。 可以在生物传感器上通过将准直的白光指向生物传感器的表面来检测生物分子相互作用。 生物传感器表面上的分子结合由来自生物传感器的反射光或透射光的峰值波长值的偏移表示，而波长的增加对应于分子吸收的增加。 可调激光光源可以产生准直的白光，并且可调滤光器可以接收反射或透射的光并将光传递到光电二极管传感器。 然后光电二极管传感器根据可调谐滤波器的调谐电压量化反射或透过可调谐滤波器的光量。

公开(公告)号：US20080246961A1

公开(公告)日：2008-10-09

申请号：US12079171

申请日：2008-03-24

申请人： Wei Zhang ,  Brian T. Cunningham

发明人： Wei Zhang ,  Brian T. Cunningham

IPC分类号： G01N21/64 ,  C03B37/00 ,  G02B6/00

CPC分类号： G01N21/648 ,  B82Y15/00 ,  B82Y20/00 ,  G01N21/7743 ,  G01N2021/7726 ,  G02B6/1226

摘要： Biosensors are disclosed which include a surface for binding to sample molecule to the biosensor in the form of a porous, thin film of dielectric material, e.g., TiO2. In one example the porous, thin film is in the form of a multitude of sub-micron sized rod-like structures (“nanorods”) projecting therefrom. In one embodiment, the biosensor is in the form of a photonic crystal biosensor. The approach of depositing a thin film of dielectric nanorods may be applied to any enhanced fluorescence biosensor surface structure, including 1-dimensional photonic crystals, 2-dimensional photonic crystals, 3-dimensional photonic crystals, surface plasmon resonance surfaces, planar waveguides, and grating-coupled waveguides. The dielectric nanorod structures can be fabricated on the surface of a biosensor by the glancing angle deposition technique (GLAD).

摘要翻译： 公开了生物传感器，其包括以多孔的电介质材料薄膜（例如TiO 2）的形式将样品分子结合到生物传感器的表面。 在一个实例中，多孔薄膜是从其突出的多个亚微米尺寸的棒状结构（“纳米棒”）的形式。 在一个实施例中，生物传感器是光子晶体生物传感器的形式。 沉积介电纳米棒薄膜的方法可以应用于任何增强的荧光生物传感器表面结构，包括一维光子晶体，二维光子晶体，三维光子晶体，表面等离子体共振表面，平面波导和光栅 耦合波导。 介电纳米棒结构可以通过扫视角沉积技术（GLAD）在生物传感器的表面上制造。

公开(公告)号：US07300814B2

公开(公告)日：2007-11-27

申请号：US11014415

申请日：2004-12-16

申请人： Brian T. Cunningham ,  Marc S. Weinberg

发明人： Brian T. Cunningham ,  Marc S. Weinberg

IPC分类号： H01L21/00

CPC分类号： B81C3/008 ,  B81B2201/0271 ,  B81C2203/031 ,  G01C19/5719 ,  G01P15/0802 ,  G01P15/097

摘要： A method of fabricating micro-mechanical devices. A mesa is etched in a homogeneous wafer. The wafer is bonded to a patterned substrate with the mesa defining device elements suspended above the substrate. A portion of the wafer is removed until a desired device thickness is achieved. Discrete elements of the device are then formed by performing a structural etch on the remaining wafer material.

摘要翻译： 一种制造微机械装置的方法。 在均匀晶片中蚀刻台面。 晶片被结合到图案化衬底，其中台面限定器件元件悬置在衬底上。 去除晶片的一部分直到实现所需的器件厚度。 然后通过对剩余的晶片材料进行结构蚀刻来形成器件的离散元件。