利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

140 条记录 (耗时 0.061 秒)

    Proteases from gram positive organisms
    55.
    发明授权
    Proteases from gram positive organisms 有权
    来自革兰氏阳性菌的蛋白酶

    公开(公告)号：US06528255B1

    公开(公告)日：2003-03-04

    申请号：US09555062

    申请日：2000-05-23

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C12Q168

    CPC分类号： C12N9/54 ,  C11D3/38663 ,  C12N9/80 ,  C12N15/75

    摘要： The present invention relates to the identification of metalloproteases in gram positive microorganisms and provides the nucleic acid and amino acid sequences for a metalloprotease. Host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease wherein the mutation or deletion results in inactivation of the proteolytic activity of the metalloprotease are also part of the invention.

    摘要翻译： 本发明涉及革兰氏阳性微生物中金属蛋白酶的鉴定，并提供金属蛋白酶的核酸和氨基酸序列。 具有编码金属蛋白酶的部分或全部基因的突变或缺失的宿主细胞，其中突变或缺失导致金属蛋白酶的蛋白水解活性失活也是本发明的一部分。

    Proteases from gram-positive organisms
    56.
    发明授权
    Proteases from gram-positive organisms 有权
    来自革兰氏阳性菌的蛋白酶

    公开(公告)号：US06521440B1

    公开(公告)日：2003-02-18

    申请号：US09486192

    申请日：2000-02-22

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C12N1511

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C12N15/75

    摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.

    摘要翻译： 本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供了用于芽孢杆菌MP的核酸和氨基酸序列。 本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的MP的清洁组合物。

    Liquid detergent with stabilized enzyme
    59.
    发明授权
    Liquid detergent with stabilized enzyme 失效
    具有稳定酶的液体洗涤剂

    公开(公告)号：US5039446A

    公开(公告)日：1991-08-13

    申请号：US559456

    申请日：1990-07-23

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C11D3/386

    CPC分类号： C11D3/38663

    摘要： Liquid detergent containing enzymes particularly proteases are stabilized against enzyme degradation prior to use by inclusion of an inhibitor of the enzyme having a dissociation constant of between about 1.times.10.sup.-2 M and about 1.times.10.sup.-7 M which binds to the enzyme such that prior to use of the detergent at least about 55% of the enzyme is bound to the enzyme inhibitor essentially at the enzyme active site. Such enzyme inhibitor so selected can be used effectively in concentrations much lower than previously taught; i.e., from about 0.002% to less than 0.1% weight/weight.

    摘要翻译： 含有酶特别是蛋白酶的液体洗涤剂在使用之前通过包含约1×10-2M至约1×10-7M的解离常数的酶的抑制剂在使用前被稳定化，其结合酶，使得在使用洗涤剂之前 至少约55％的酶基本上在酶活性位点与酶抑制剂结合。 所选择的这种酶抑制剂可以有效地以比以前教导的浓度低得多的方式使用; 即约0.002％至小于0.1％重量/重量。