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    Polymerase-encoding nucleic acids and method of making and using them
    56.
    发明授权
    Polymerase-encoding nucleic acids and method of making and using them 有权
    聚合酶编码核酸及其制备和使用方法

    公开(公告)号：US07781198B2

    公开(公告)日：2010-08-24

    申请号：US10034621

    申请日：2001-12-21

    申请人： Walter Callen ,  Eric J. Mathur

    发明人： Walter Callen ,  Eric J. Mathur

    IPC分类号： C12N9/12 ,  C07H21/02

    CPC分类号： C12N9/1252 ,  A01K2217/05

    摘要： The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.

    摘要翻译： 本发明涉及聚合酶活性温度在90℃至113℃范围内的热稳定聚合酶，例如源自富马酸火焰菌的那些，以及编码聚合酶的多核苷酸。此外，设计新的耐热DNA聚合酶的方法 还提供了其使用方法。 聚合酶在增加的pH和温度下具有增加的活性和稳定性。

    Esterases and Related Nucleic Acids and Methods
    57.
    发明申请
    Esterases and Related Nucleic Acids and Methods 审中-公开
    酯酶和相关的核酸和方法

    公开(公告)号：US20090324574A1

    公开(公告)日：2009-12-31

    申请号：US12278108

    申请日：2007-02-02

    申请人： Eric J. Mathur ,  Walter N. Callen ,  Roderick Fielding

    发明人： Eric J. Mathur ,  Walter N. Callen ,  Roderick Fielding

    IPC分类号： A61K38/46 ,  C12N15/11 ,  C07H21/04 ,  C12N15/00 ,  C12N1/21 ,  C12N1/19 ,  C12N9/16 ,  C12P21/02 ,  C07H1/00 ,  C12P7/64 ,  C12P7/10 ,  D06M16/00 ,  A23L2/38 ,  A23L1/00 ,  A23K1/00 ,  A61K38/02 ,  A61P1/14

    CPC分类号： C12N9/14 ,  Y02E50/16

    摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphplipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.

    摘要翻译： 本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如磷脂酰酶A，B，C和D活性，patatin活性，脂质酰基水解酶（ LAH）活性）或蛋白酶活性，包括耐热和耐热水解酶活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换）， 酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。 本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。 另一方面，本发明的多肽用于合成对映体纯的手性产物。

    Method of making a protein polymer and uses of the polymer
    58.
    发明授权
    Method of making a protein polymer and uses of the polymer 失效
    制备蛋白质聚合物的方法和聚合物的用途

    公开(公告)号：US07459172B2

    公开(公告)日：2008-12-02

    申请号：US09997807

    申请日：2001-11-30

    申请人： Jay Short ,  Eric J. Mathur ,  W. Michael Lafferty ,  Nelson Barton ,  Kevin Chow

    发明人： Jay Short ,  Eric J. Mathur ,  W. Michael Lafferty ,  Nelson Barton ,  Kevin Chow

    IPC分类号： A61K31/74 ,  A61K38/16

    CPC分类号： B82Y30/00 ,  A61K9/0092 ,  A61K9/1274 ,  C07K14/195

    摘要： A polymer is prepared by self-assembly of a plurality of monomeric polypeptide units. The polymer tends to form a nanotube and is capable of encapsulating a particular drug molecule. Once encapsulated in the polymer of the present invention, the drug molecule may be delivered to a particular location of human body to effectively cure a disease or treat a symptom.Generally, the monomeric polypeptide unit of the present invention has a sequence found in Pyrodictium abyssi, a microorganism that produces an extracellular network having hollow protein tubes, or a sequence substantially identical thereto. The monomeric polypeptide may be mass produced using recombinant biotechnologies and be polymerized into the polymer of the present invention. One or more additional targeting vector may be attached to the monomeric polypeptide unit or the polymer to facilitate the targeting of the drug molecule that may be held there within. The sequence contained in the monomeric polypeptide unit may be further optimized using one or more technique selected from Gene Site Saturation Mutagenesis and GeneReasembly™.

    摘要翻译： 通过多个单体多肽单元的自组装制备聚合物。 聚合物倾向于形成纳米管并且能够封装特定的药物分子。 一旦包封在本发明的聚合物中，药物分子可以被递送到人体的特定位置以有效治愈疾病或治疗症状。 通常，本发明的单体多肽单元具有在黑腹果蝇（Pyrodictium abyssi）中发现的序列，该菌株产生具有中空蛋白管的细胞外网络或与其基本相同的序列。 单体多肽可以使用重组生物技术大规模生产并聚合成本发明的聚合物。 一个或多个另外的靶向载体可以连接到单体多肽单元或聚合物，以促进可能在其中保持的药物分子的靶向。 可以使用选自Gene Site Saturation Mutagenesis和GeneReasembly TM的一种或多种技术进一步优化单体多肽单元中所含的序列。