利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

63 条记录 (耗时 0.067 秒)

    Isolated dishevelled associated kinases, polynucleotides encoding the kinases, and methods of use thereof
    56.
    发明授权
    Isolated dishevelled associated kinases, polynucleotides encoding the kinases, and methods of use thereof 失效
    分离的蓬乱相关激酶，编码激酶的多核苷酸及其使用方法

    公开(公告)号：US06958238B2

    公开(公告)日：2005-10-25

    申请号：US10464939

    申请日：2003-06-18

    申请人： Tian-Qiang Sun ,  Lewis T. Williams

    发明人： Tian-Qiang Sun ,  Lewis T. Williams

    IPC分类号： G01N33/50 ,  A61K31/7088 ,  A61K38/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P35/00 ,  C12N9/12 ,  C12N15/09 ,  C12N15/54 ,  G01N33/15

    CPC分类号： C12N9/12

    摘要： The invention provides Dishevelled Associated Kinase polypeptides, which phosphorylate Dsh proteins involved in the Wnt/Wg signalling pathway. Isolated polynucleotides encoding DAK polypeptides are further provided, as are recombinant vectors, and host cells comprising the polynucleotides. Methods of using the polynucleotides and polypeptides of the invention are further aspects of the invention, including assays to detect agents which modulate DAK kinase activity. Further provided are methods of modulating pathways involved in control of cell proliferation.

    摘要翻译： 本发明提供了Dishevelled Associated Kinase多肽，其使参与Wnt / Wg信号通路的Dsh蛋白质磷酸化。 还提供编码DAK多肽的分离的多核苷酸，重组载体以及包含多核苷酸的宿主细胞也是如此。 使用本发明的多核苷酸和多肽的方法是本发明的另外的方面，包括检测调节DAK激酶活性的试剂的测定法。 进一步提供调节参与控制细胞增殖的途径的方法。

    Receptors for fibroblast growth factors
    57.
    发明授权
    Receptors for fibroblast growth factors 失效
    成纤维细胞生长因子受体

    公开(公告)号：US06350593B1

    公开(公告)日：2002-02-26

    申请号：US08459395

    申请日：1995-06-02

    申请人： Lewis T. Williams ,  Daniel F. Johnson ,  Pauline E Lee

    发明人： Lewis T. Williams ,  Daniel F. Johnson ,  Pauline E Lee

    IPC分类号： C12N1509

    CPC分类号： C07K14/71 ,  A61K38/00 ,  G01N33/74 ,  G01N2333/50 ,  G01N2333/71 ,  G01N2500/00

    摘要： A fibroblast growth factor (FGF) receptor including a basic fibroblast growth factor receptor has been purified. Various forms have bee identified including soluble forms lacking any transmembrane segment. DNA sequences encoding full-length fibroblast growth factor receptors and polypeptides comprising a portion of an FGF-R ligand-binding domain have been isolated and sequenced. These DNAs include DNAs encoding for a basic FGF-R and a human FGF-R and are operably linked to control sequences and expressed in a culture of a compatible host transformed, transfected or electroporated by a cloning vehicle containing the DNA sequence. The invention also comprises antibodies to the receptor, methods of synthesizing the growth factor receptor proteins, methods for providing analogs of the fibroblast growth factor receptors. Methods for evaluating compositions which promoter or inhibit fibroblastic growth factors and compositions which are agonistic or antagonistic to fibroblast growth factor receptors are also provided. Diagnostic and therapeutic uses are described.

    摘要翻译： 已经纯化了包含碱性成纤维细胞生长因子受体的成纤维细胞生长因子（FGF）受体。 鉴别出各种形式的蜜蜂，包括没有任何跨膜片段的可溶形式。 已经分离并测序了编码全长成纤维细胞生长因子受体的DNA序列和包含一部分FGF-R配体结合结构域的多肽。 这些DNA包括编码碱性FGF-R和人FGF-R的DNA，并且与对照序列可操作地连接，并在通过含有DNA序列的克隆载体转化，转染或电穿孔的相容宿主的培养物中表达。 本发明还包括对受体的抗体，合成生长因子受体蛋白质的方法，提供成纤维细胞生长因子受体类似物的方法。 还提供了用于评估启动子或抑制成纤维细胞生长因子和对成纤维细胞生长因子受体具有激动作用或拮抗作用的组合物的组合物的方法。 描述诊断和治疗用途。

    Constitutively active phosphatidylinositol 3-kinase and uses thereof
    58.
    发明授权
    Constitutively active phosphatidylinositol 3-kinase and uses thereof 失效
    组成型活性磷脂酰肌醇3-激酶及其用途

    公开(公告)号：US6043062A

    公开(公告)日：2000-03-28

    申请号：US390874

    申请日：1995-02-17

    申请人： Anke Klippel ,  Lewis T. Williams

    发明人： Anke Klippel ,  Lewis T. Williams

    IPC分类号： A61K38/00 ,  C12N9/12 ,  C12N15/54 ,  C12P9/00

    CPC分类号： C12N9/1205 ,  A61K38/45 ,  C07H21/04 ,  Y10S435/975

    摘要： The invention provides a method of producing a constitutively active phosphatidylinositol 3-kinase (PI 3-kinase) comprising the catalytic p110 subunit covalently attached at the N-terminus to the iSH2 region of the regulatory subunit, p85. The invention discloses one form of the constitutively active kinase, p110*, which functions independently of growth factor stimulation. Expression vectors encoding a constitutively active PI 3-kinase and cells containing such expression vectors are provided. The invention also provides methods of using the constitutively active phosphatidylinositol 3-kinase to generate phosphoinositides, to identify cellular target proteins and associating molecules of PI 3-kinase, to screen for inhibitors of PI 3-kinase activity and to treat certain diseases, in particular, proliferative diseases. Kits comprising the constitutively active kinase are also provided.

    摘要翻译： 本发明提供了一种生产组成型活性磷脂酰肌醇3-激酶（PI3-激酶）的方法，其包含在N末端与调节亚基p85的iSH2区共价连接的催化性p110亚基。 本发明公开了一种形式的组成型活性激酶p110 *，其独立于生长因子刺激起作用。 提供编码组成型活性PI 3激酶的表达载体和含有这种表达载体的细胞。 本发明还提供使用组成型活性磷脂酰肌醇3-激酶产生磷酸肌醇，鉴定细胞靶蛋白和结合PI3-激酶分子的筛选PI3-激酶活性抑制剂和治疗某些疾病的方法，特别是 ，增殖性疾病。 还提供了包含组成型活性激酶的试剂盒。

    Nucleic acid encoding novel protein domain which binds tyrosine phosphorylated proteins
    60.
    发明授权
    Nucleic acid encoding novel protein domain which binds tyrosine phosphorylated proteins 失效
    编码结合酪氨酸磷酸化蛋白质的新型蛋白质结构域的核酸

    公开(公告)号：US5925547A

    公开(公告)日：1999-07-20

    申请号：US551687

    申请日：1995-11-01

    申请人： Lewis T. Williams ,  William Michael Kavanaugh

    发明人： Lewis T. Williams ,  William Michael Kavanaugh

    IPC分类号： G01N33/566 ,  A61K38/00 ,  A61K48/00 ,  A61P9/10 ,  A61P17/00 ,  A61P29/00 ,  A61P35/00 ,  A61P43/00 ,  C07K14/47 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12Q1/68

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： The present invention generally relates to novel protein domains which bind to tyrosine-phosphorylated proteins. More particularly, the present invention provides non-SH2 protein domains that bind phosphotyrosine. The present invention also provides various composite polypeptides comprising these domains, such that these composite polypeptides retain the ability to bind tyrosine phosphorylated proteins. It also provides nucleic acids encoding these polypeptides. Typically, these nucleic acids may also comprise a promoter for expression, and a segment encoding fusion peptides on the amino or carboxy terminus of the expressed composite protein. Also included within the present invention are methods of preparing these polypeptides and cells capable of expressing them. Also provided are methods of using these polypeptides in research, diagnostic and therapeutic applications.

    摘要翻译： 本发明一般涉及结合酪氨酸磷酸化蛋白质的新型蛋白质结构域。 更具体地，本发明提供了结合磷酸酪氨酸的非SH2蛋白结构域。 本发明还提供了包含这些结构域的各种复合多肽，使得这些复合多肽保留结合酪氨酸磷酸化蛋白的能力。 它还提供编码这些多肽的核酸。 通常，这些核酸还可以包含用于表达的启动子，以及在表达的复合蛋白的氨基或羧基末端编码融合肽的区段。 本发明还包括制备这些多肽和能够表达它们的细胞的方法。 还提供了在研究，诊断和治疗应用中使用这些多肽的方法。