公开(公告)号：US11905519B2

公开(公告)日：2024-02-20

申请号：US17815122

申请日：2022-07-26

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/50 ,  A01N47/08

CPC classification number: C12N15/8286 ,  A01N47/08 ,  A01N63/50 ,  C07K14/325 ,  Y02A40/146 ,  A01N63/50 ,  A01N63/23

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US11459359B2

公开(公告)日：2022-10-04

申请号：US17127310

申请日：2020-12-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Artem G. Evdokimov ,  Farhad Moshiri ,  Timothy J. Rydel ,  Eric J. Sturman ,  Moritz von Rechenberg ,  Halong Vu ,  Andrew M. Wollacott ,  Meiying Zheng

IPC: C12N1/20 ,  C07K14/325 ,  C12N15/82 ,  C07K14/00 ,  A01N37/46 ,  C07K14/435 ,  C07K14/32

Abstract: The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

公开(公告)号：US20220281927A1

公开(公告)日：2022-09-08

申请号：US17698936

申请日：2022-03-18

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C07K14/325 ,  C12N15/82 ,  A01N37/46

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US10801037B2

公开(公告)日：2020-10-13

申请号：US16573538

申请日：2019-09-17

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/10 ,  A01N47/08

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US10787680B2

公开(公告)日：2020-09-29

申请号：US16716281

申请日：2019-12-16

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Larry A. Gilbertson ,  David K. Kovalic ,  Thomas J. Larosa ,  Maolong Lu ,  Tichafa R. I. Munyikwa ,  James K. Roberts ,  Wei Wu ,  Bei Zhang

IPC: C12N15/82 ,  A01N63/10

Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US10703782B2

公开(公告)日：2020-07-07

申请号：US15868676

申请日：2018-01-11

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  David J. Bowen ,  Catherine A. Chay ,  David J. Chi ,  William P. Clinton ,  Crystal L. Dart ,  Leigh English ,  Stanislaw Flasinski ,  Victor M. Guzov ,  Kevin A. Jarrell ,  Uma R. Kesanapalli ,  Thomas M. Malvar ,  Robert M. McCarroll ,  Jason S. Milligan ,  Jay P. Morgenstern ,  Deborah G. Rucker ,  Sara A. Salvador ,  Temple F. Smith ,  Carlos E. Soto ,  Collin M. Stultz ,  Brian M. Turczyk ,  Ty T. Vaughn ,  Moritz W. F. Von Rechenberg

IPC: C07K14/325 ,  C12N15/82

Abstract: Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

公开(公告)号：US10538783B2

公开(公告)日：2020-01-21

申请号：US15610220

申请日：2017-05-31

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Claire A. CaJacob ,  Pascale Feldmann ,  Gregory R. Heck ,  Irene Maria Antonia Nooren ,  Geert Plaetinck ,  Wendy T. Maddelein ,  Ty T. Vaughn

IPC: C12N15/82 ,  C07H21/04 ,  C07K14/435 ,  C12N15/113

Abstract: The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US10334858B2

公开(公告)日：2019-07-02

申请号：US15729114

申请日：2017-10-10

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: C07H21/04 ,  C12N15/67 ,  A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N15/72 ,  C12N9/22

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US10287605B2

公开(公告)日：2019-05-14

申请号：US15835346

申请日：2017-12-07

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: A01N47/08 ,  A01N63/02 ,  C12N15/82 ,  C07K14/325

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US10070652B2

公开(公告)日：2018-09-11

申请号：US15237214

申请日：2016-08-15

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/00 ,  A01N65/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  A01N63/02 ,  C12N15/67 ,  A01H3/00 ,  C12N15/72 ,  C12N9/22

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.