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    Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis
    61.
    发明授权
    Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis 失效
    寡核苷酸标记核苷三磷酸（OTNTP）用于遗传分析

    公开(公告)号：US07109316B2

    公开(公告)日：2006-09-19

    申请号：US10226734

    申请日：2002-08-23

    Applicant: Shiv Kumar ,  Anup Sood ,  Sudhakar Rao ,  John Nelson

    Inventor： Shiv Kumar ,  Anup Sood ,  Sudhakar Rao ,  John Nelson

    IPC: C07H21/02 ,  C07H21/04 ,  C07H19/04 ,  C12Q1/68

    CPC classification number: C07H19/04

    Abstract: Oligonucleotide tagged nucleoside triphosphates, OTNTPs, which are substrates for polymerases and or terminal nucleotidyl transferases are provided as well as methods of making these OTNTPs. Further provided are OTNTPs with fluorescent dyes including energy transfer dyes, attached to the oligonucleotide chain, OTNTPs with unnatural bases incorporated in the oligonucleotide sequence and methods for incorporating these OTNTPs in DNA or RNA. Also provided are methods for using the oligonucleotides on OTNTPs for amplifying the oligo sequence on the OTNTP using an amplification method described above. Further provided are reactive bifunctional amidites, methods of making these compounds and methods for detecting single nucleotide polymorphisms using the above OTNTPs. Methods for detecting differential gene expression using the OTNTPs and methods of separating specifically modified DNA or RNA using the OTNTPs are also provided.

    Abstract translation: 提供了寡聚核苷酸标记的核苷三磷酸酯，OTNTP，其是用于聚合酶和末端核苷酸转移酶的底物，以及制备这些OTNTP的方法。 还提供了含有荧光染料的OTNTP，其包括与寡核苷酸链连接的能量转移染料，掺入寡核苷酸序列中的非天然碱基的OTNTP以及将这些OTNTP引入DNA或RNA中的方法。 还提供了使用寡核苷酸在OTNTP上使用上述扩增方法在OTNTP上扩增寡核苷酸序列的方法。 还提供了反应性双官能脒，制备这些化合物的方法和使用上述OTNTP检测单核苷酸多态性的方法。 还提供了使用OTNTP检测差异基因表达的方法和使用OTNTP分离特异性修饰的DNA或RNA的方法。

    Pyrimidinedione derivatives
    62.
    发明授权
    Pyrimidinedione derivatives 失效
    嘧啶二酮衍生物

    公开(公告)号：US07101873B2

    公开(公告)日：2006-09-05

    申请号：US10409153

    申请日：2003-04-09

    Applicant: Shiv Kumar Agarwal ,  Ravikumar Tadiparthi ,  Pawan Aggarwal ,  Savithiri Shivakumar

    Inventor： Shiv Kumar Agarwal ,  Ravikumar Tadiparthi ,  Pawan Aggarwal ,  Savithiri Shivakumar

    IPC: A61K31/54

    CPC classification number: C07D239/54

    Abstract: Pyrimidinedione derivatives of the general formula (I), their derivatives, analogs, tautomeric forms, stereoisomers, polymorphs, hydrates, solvates, pharmaceutically acceptable salts and pharmaceutically acceptable compositions containing them are useful for the treatment of inflammation and immunological diseases

    Abstract translation: 通式（I）的嘧啶二酮衍生物，其衍生物，类似物，互变异构体形式，立体异构体，多晶型物，水合物，溶剂合物，药学上可接受的盐和含有它们的药学上可接受的组合物可用于治疗炎症和免疫疾病

    Terminal-phosphate-labeled nucleotides and methods of use
    64.
    发明授权
    Terminal-phosphate-labeled nucleotides and methods of use 有权

    公开(公告)号：US07052839B2

    公开(公告)日：2006-05-30

    申请号：US10113030

    申请日：2002-04-01

    Applicant: John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

    Inventor： John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

    IPC: C12Q1/68

    CPC classification number: C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6851

    Abstract: The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

    Energy transfer dyes, terminators and use thereof
    65.
    发明申请
    Energy transfer dyes, terminators and use thereof 审中-公开
    能量转移染料，终止剂及其用途

    公开(公告)号：US20050255475A1

    公开(公告)日：2005-11-17

    申请号：US10528863

    申请日：2003-09-25

    Applicant: Shiv Kumar ,  Chung-Yuan Chen ,  Sudhakar Rao

    Inventor： Shiv Kumar ,  Chung-Yuan Chen ,  Sudhakar Rao

    IPC: C07D311/82 ,  C07D405/14 ,  C07D473/02 ,  C07D487/04 ,  C07D491/22 ,  C09B11/04 ,  C09B11/08 ,  C09B11/24 ,  C12Q1/68

    CPC classification number: C09B11/08 ,  C07D311/82 ,  C07D405/14 ,  C07D487/04 ,  C09B11/04 ,  C09B11/24 ,  C12Q1/6869 ,  C12Q2527/125

    Abstract: The present invention relates to a novel set of four fluorescently labeled dye terminators. Two of them are single dye labeled terminators and the other two dye terminators are based on fluorescent resonance energy transfer (FRET). The FRET dye terminators are generated from the 4′,5′-bis-aminomethylfluorescein. Of the two amino groups of the donor dye, 4′,5′-bis-aminomethylfluorescein, one amino group is used to attach the acceptor dye, and the other amino group is used to attach the dideoxynucleoside-5′-triphosphate. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

    Abstract translation: 本发明涉及一组四种荧光标记的染料终止剂。 其中两个是单染色标记终止子，另外两个染料终止子是基于荧光共振能量转移（FRET）。 FRET染料终止子由4'，5'-双氨基甲基荧光素生成。 在供体染料的两个氨基中，4'，5'-双氨基甲基荧光素，1个氨基用于连接受体染料，而另一个氨基用于连接双脱氧核苷-5'-三磷酸。 公开了它们的制备，能量转移效率和用作标记，特别是在DNA测序反应中。

    Tissue culture process for producing a large number of viable mint plants in vitro from internodal segments
    66.
    发明授权
    Tissue culture process for producing a large number of viable mint plants in vitro from internodal segments 失效
    组织培养方法，用于在体外从节间段产生大量可行的薄荷植物

    公开(公告)号：US5898001A

    公开(公告)日：1999-04-27

    申请号：US792545

    申请日：1997-01-31

    Applicant: Sushil Kumar ,  Shiv Kumar Gupta ,  Savithri Bhat ,  Rakesh Tuli

    Inventor： Sushil Kumar ,  Shiv Kumar Gupta ,  Savithri Bhat ,  Rakesh Tuli

    IPC: A01H4/00 ,  C12N5/04 ,  C12N5/00

    CPC classification number: A01H4/008

    Abstract: The present invention relates to a tissue culture process for producing a large number of viable mint plants in vitro. The process of the present invention employs specified pieces of an internodal segment of the stem of the mint plant as the starting material and identifies medium and culture conditions for producing a large number of plants. Such plants can be used for micropropagation, selection of mutants, production of plants with altered levels of endogenous secondary metabolites and for genetic engineering.

    Abstract translation: 本发明涉及用于在体外生产大量活的薄荷植物的组织培养方法。 本发明的方法使用薄荷植物的茎的节间段的指定片段作为起始材料，并且识别用于生产大量植物的培养条件。 这样的植物可以用于微繁殖，突变体的选择，产生具有改变的内源性次级代谢物水平的植物和遗传工程。

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