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公开(公告)号：US4861448A
公开(公告)日：1989-08-29
申请号：US99535
申请日：1987-09-22
Applicant: Charles R. Cantor , David C. Schwartz
Inventor： Charles R. Cantor , David C. Schwartz
IPC: G01N27/447
CPC classification number: G01N27/44743 , G01N27/44773
Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
Abstract translation: 包含适合用于电泳方法的固化液体如琼脂糖的凝胶插入物，包埋在由固化液体形成的基质中的裂解细胞和大分子如DNA或来自裂解细胞的完整染色体可以有利地用于电泳分离。 将凝胶插入物直接放置在合适的载体介质中并经受一个或多个电场以分离大分子。
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公开(公告)号：US4695548A
公开(公告)日：1987-09-22
申请号：US654641
申请日：1984-09-25
Applicant: Charles R. Cantor , David C. Schwartz
Inventor： Charles R. Cantor , David C. Schwartz
IPC: G01N27/447 , C12N11/12
CPC classification number: G01N27/44773 , Y10T428/2984 , Y10T428/2987
Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
Abstract translation: 包含适合用于电泳方法的固化液体如琼脂糖的凝胶插入物，包埋在由固化液体形成的基质中的裂解细胞和大分子如DNA或来自裂解细胞的完整染色体可以有利地用于电泳分离。 将凝胶插入物直接放置在合适的载体介质中并经受一个或多个电场以分离大分子。
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公开(公告)号：US09534224B2
公开(公告)日：2017-01-03
申请号：US10535128
申请日：2003-11-14
Applicant: James J. Collins , Farren J. Isaacs , Charles R. Cantor , Daniel J. Dwyer
Inventor： James J. Collins , Farren J. Isaacs , Charles R. Cantor , Daniel J. Dwyer
CPC classification number: C12N15/67 , C12N15/11 , C12N2310/53 , C12Q1/6897
Abstract: The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5′ untranslated region (5′ UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5′ UTR of an mRNA. A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously repressed state.
Abstract translation: 本发明提供核酸分子，DNA构建体，质粒和用于转录后调节基因表达的方法，其使用RNA分子抑制和激活开放阅读框的翻译。 通过在mRNA分子的5'非翻译区（5'UTR）内存在调节性核酸元件（顺式抑制性RNA或crRNA）来实现基因表达的抑制。 核酸元件通过互补碱基配对形成发夹（茎/环）结构。 发夹阻止核糖体进入mRNA转录物，从而阻止翻译。 特别地，在设计用于在原核细胞中操作的本发明的实施方案中，发夹二级结构的茎螯合核糖体结合位点（RBS）。 在设计用于在真核细胞中操作的本发明的实施方案中，发夹的茎位于起始密码子的上游，位于mRNA的5'UTR内的任何位置。 以反式表达的小RNA（反式激活RNA或taRNA）与crRNA相互作用并改变发夹结构。 这种改变允许核糖体进入起始密码子上游的转录物区域，从而从其先前的抑制状态激活转录。
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64.发明授权公开(公告)号：US09034580B2
公开(公告)日：2015-05-19
申请号：US12354749
申请日：2009-01-15
Applicant: Charles R. Cantor
Inventor： Charles R. Cantor
CPC classification number: C12Q1/6876 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844 , C12Q2565/537 , C12Q2565/519 , C12Q2565/501 , C12Q2531/101 , C12Q2525/161 , C12Q2525/155
Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
Abstract translation: 本文提供了改进的固体支持物和分析靶核苷酸序列的方法。 某些改进旨在有效地制备包含与一个或多个靶核苷酸序列或其互补序列相同或基本相同的核苷酸序列的核酸。 所制备的核酸包括促进序列分析的参考序列。 本文提供的固体支持物和方法使发布的序列分析方法所需的步骤数量最小化，从而提供改进的序列分析效率。
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公开(公告)号：US20110263453A1
公开(公告)日：2011-10-27
申请号：US13129797
申请日：2009-11-20
Applicant: Charles R. Cantor
Inventor： Charles R. Cantor
Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.
Abstract translation: 本文描述的是用于核酸定量的产物和方法，其部分可用于检测和测定样品中稀有核酸（即低拷贝数核酸）的核苷酸序列。 这样的产物和方法可用于减少不同核酸物种之间的动态范围。
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Patent Agency Ranking
公开(公告)号：US08034567B2
公开(公告)日：2011-10-11
申请号：US10655762
申请日：2003-09-05
Applicant: Charles R. Cantor , Chunming Ding
Inventor： Charles R. Cantor , Chunming Ding
CPC classification number: C12Q1/6851 , C12Q2545/107 , C12Q2535/125 , C12Q2525/186
Abstract: The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.
Abstract translation: 本发明涉及使用与目的基因相比具有一个碱基差异的标准或“靶核酸序列”来测量样品中靶核酸量的方法。使用这种标准物 结合使用例如在突变位点上携带的碱基延伸反应来“提高”标准差和测试核酸样品的方法，允许以相同的效率扩增标准品和靶核酸并促进定量 的靶核酸。 此后，使用定量“增强”标准和靶核酸样品的手段来确定靶核酸的量。 在优选实施方案中，定量方法是质谱法。
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公开(公告)号：US20100184075A1
公开(公告)日：2010-07-22
申请号：US12704043
申请日：2010-02-11
Applicant: Charles R. Cantor , Chunming Ding
Inventor： Charles R. Cantor , Chunming Ding
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C12Q2527/137 , C12Q2537/143 , C12Q2565/627
Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.
Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。
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68.发明申请公开(公告)号：US20090220942A1
公开(公告)日：2009-09-03
申请号：US12091709
申请日：2006-10-27
Applicant: Natalia Broude , Charles R. Cantor , Vadim V. Demidov
Inventor： Natalia Broude , Charles R. Cantor , Vadim V. Demidov
CPC classification number: C12Q1/6883
Abstract: The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.
Abstract translation: 本发明涉及一种产生活化的裂多肽片段的方法，其在重构时立即形成活性蛋白质。 该方法涉及实时蛋白质互补。 本发明还包括分裂和产生活性状态的分裂荧光蛋白的方法，其在重建时立即产生荧光信号。 本申请还提供了检测核酸的方法; 非核酸分析物和核酸杂交，使用本发明的新型活化的裂解多肽片段。
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69.发明申请公开(公告)号：US20090202984A1
公开(公告)日：2009-08-13
申请号：US12354749
申请日：2009-01-15
Applicant: Charles R. Cantor
Inventor： Charles R. Cantor
CPC classification number: C12Q1/6876 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844 , C12Q2565/537 , C12Q2565/519 , C12Q2565/501 , C12Q2531/101 , C12Q2525/161 , C12Q2525/155
Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
Abstract translation: 本文提供了改进的固体支持物和分析靶核苷酸序列的方法。 某些改进旨在有效地制备包含与一个或多个靶核苷酸序列或其互补序列相同或基本相同的核苷酸序列的核酸。 所制备的核酸包括促进序列分析的参考序列。 本文提供的固体支持物和方法使发布的序列分析方法所需的步骤数量最小化，从而提供改进的序列分析效率。
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70.发明申请公开(公告)号：US20090029370A1
公开(公告)日：2009-01-29
申请号：US12091682
申请日：2006-10-27
Applicant: Natalia E. Broude , Charles R. Cantor , Maria A. Burton , Vadim V. Demidov
Inventor： Natalia E. Broude , Charles R. Cantor , Maria A. Burton , Vadim V. Demidov
CPC classification number: C12Q1/6816 , C12Q1/682 , C12Q2563/107 , C12Q2561/107 , C12Q2522/101
Abstract: The present invention relates to a method to detect nucleic acid molecules, such as RNA molecules in vivo using real time protein complementation methods. The invention further relates to methods for detecting nucleic acids, for example RNA in real-time in living cells with a high sensitivity, using a novel split biomolecular conjugate of the invention.
Abstract translation: 本发明涉及使用实时蛋白质互补方法在体内检测核酸分子例如RNA分子的方法。 本发明还涉及使用本发明的新的分裂生物分子缀合物来检测核酸，例如用活性细胞实时检测高灵敏度的核酸的方法。
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