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    Electrophoretic methods employing gel inserts
    1.
    发明授权
    Electrophoretic methods employing gel inserts 失效
    使用凝胶插入物的电泳方法

    公开(公告)号：US4861448A

    公开(公告)日：1989-08-29

    申请号：US99535

    申请日：1987-09-22

    Applicant: Charles R. Cantor ,  David C. Schwartz

    Inventor： Charles R. Cantor ,  David C. Schwartz

    IPC: G01N27/447

    CPC classification number: G01N27/44743 ,  G01N27/44773

    Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.

    Abstract translation: 包含适合用于电泳方法的固化液体如琼脂糖的凝胶插入物，包埋在由固化液体形成的基质中的裂解细胞和大分子如DNA或来自裂解细胞的完整染色体可以有利地用于电泳分离。 将凝胶插入物直接放置在合适的载体介质中并经受一个或多个电场以分离大分子。

    Gel inserts useful in electrophoresis
    2.
    发明授权
    Gel inserts useful in electrophoresis 失效
    凝胶插片可用于电泳

    公开(公告)号：US4695548A

    公开(公告)日：1987-09-22

    申请号：US654641

    申请日：1984-09-25

    Applicant: Charles R. Cantor ,  David C. Schwartz

    Inventor： Charles R. Cantor ,  David C. Schwartz

    IPC: G01N27/447 ,  C12N11/12

    CPC classification number: G01N27/44773 ,  Y10T428/2984 ,  Y10T428/2987

    Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.

    Abstract translation: 包含适合用于电泳方法的固化液体如琼脂糖的凝胶插入物，包埋在由固化液体形成的基质中的裂解细胞和大分子如DNA或来自裂解细胞的完整染色体可以有利地用于电泳分离。 将凝胶插入物直接放置在合适的载体介质中并经受一个或多个电场以分离大分子。

    Electrophoresis using alternating transverse electric fields
    3.
    发明授权
    Electrophoresis using alternating transverse electric fields 失效
    使用交替横向电场进行电泳

    公开(公告)号：US4473452A

    公开(公告)日：1984-09-25

    申请号：US442580

    申请日：1982-11-18

    Applicant: Charles R. Cantor ,  David C. Schwartz

    Inventor： Charles R. Cantor ,  David C. Schwartz

    IPC: G01N27/26 ,  B01D20060101 ,  B01D57/02 ,  G01N20060101 ,  G01N27/447 ,  G01N27/28

    CPC classification number: G01N27/44773

    Abstract: Disclosed are an apparatus for and a method of electrophoretically separating particles by electric fields which are transverse to each other, which alternate between respective high and low intensities out of phase with each other at a frequency related to the mass of the particles and which move the particles in an overall direction transverse to the respective directions of the fields. For separating large macromolecules, at least one of the fields preferably has an intensity gradient in a direction transverse to its own. The new arrangement makes it possible to: (1) separate particles (molecules) larger in size than those able to be separated with previously known techniques, (2) carry out separation at higher speed and at better resolution than is possible with previously known techniques, and (3) concurrently separate particles which differ greatly in mass (molecular weight).

    Abstract translation: 公开了一种通过电场电泳分离颗粒的装置和方法，它们彼此横向，它们以相对于颗粒的质量的频率彼此相位的相应的高和低强度之间交替， 颗粒在横向于各个方向的整个方向上。 为了分离大的大分子，至少一个场优选在横向于其自身的方向上具有强度梯度。 新的布置使得可以：（1）分离比以前已知技术更大尺寸的颗粒（分子），（2）以比先前已知技术更高的速度和更好的分辨率进行分离 ，（3）同时分离质量差异很大的分子（分子量）。

    METHOD FOR DETECTING AND QUANTIFYING RARE MUTATIONS/POLYMORPHISMS
    4.
    发明申请
    METHOD FOR DETECTING AND QUANTIFYING RARE MUTATIONS/POLYMORPHISMS 有权
    检测和量化稀有突变/多态性的方法

    公开(公告)号：US20110097712A1

    公开(公告)日：2011-04-28

    申请号：US12496390

    申请日：2009-07-01

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: C12Q1/68

    CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

    Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

    Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

    METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION
    5.
    发明申请
    METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION 有权
    在核酸中检测序列的方法：通过分段进行的目标序列

    公开(公告)号：US20090075288A1

    公开(公告)日：2009-03-19

    申请号：US12259376

    申请日：2008-10-28

    Applicant: Charles R. Cantor ,  Fouad A. Siddiqi

    Inventor： Charles R. Cantor ,  Fouad A. Siddiqi

    IPC: C12Q1/68

    CPC classification number: C12Q1/6872 ,  C12Q2525/101 ,  C12Q2523/107

    Abstract: The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.

    Abstract translation: 本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。 该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。 该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。 这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。 这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。

    Method for De Novo Detection of Sequences in Nucleic Acids: Target Sequencing by Fragmentation
    6.
    发明申请
    Method for De Novo Detection of Sequences in Nucleic Acids: Target Sequencing by Fragmentation 失效
    核酸序列的新检测方法：通过分段进行靶序列测定

    公开(公告)号：US20080096194A1

    公开(公告)日：2008-04-24

    申请号：US11547765

    申请日：2005-04-08

    Applicant: Charles R. Cantor ,  Fouad A. Siddiqi

    Inventor： Charles R. Cantor ,  Fouad A. Siddiqi

    IPC: C12Q1/68

    CPC classification number: C12Q1/6872 ,  C12Q2525/101 ,  C12Q2523/107

    Abstract: The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.

    Abstract translation: 本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。 该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。 该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。 这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。 这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。

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