利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

91 条记录 (耗时 0.046 秒)

    Proteinaceous pharmaceuticals and uses thereof
    64.
    发明申请
    Proteinaceous pharmaceuticals and uses thereof 审中-公开
    蛋白质药物及其用途

    公开(公告)号：US20070212703A1

    公开(公告)日：2007-09-13

    申请号：US11528950

    申请日：2006-09-27

    申请人： Willem Stemmer ,  Volker Schellenberger ,  Martin Bader ,  Michael Scholle

    发明人： Willem Stemmer ,  Volker Schellenberger ,  Martin Bader ,  Michael Scholle

    IPC分类号： C40B40/02 ,  C40B40/08 ,  C40B40/10

    CPC分类号： G01N33/6845 ,  C07K14/001 ,  C07K14/415 ,  C12N15/1044

    摘要： The present invention provides cysteine-containing scaffolds and/or proteins, expression vectors, host cell and display systems harboring and/or expressing such cysteine-containing products. The present invention also provides methods of designing libraries of such products, methods of screening such libraries to yield entities exhibiting binding specificities towards a taraget molecule. Further provided by the invention are pharmaceutical compositions comprising the cysteine-containing products of the present invention.

    摘要翻译： 本发明提供含半胱氨酸的支架和/或蛋白质，表达载体，宿主细胞和含有和/或表达这种含半胱氨酸的产品的显示系统。 本发明还提供了设计此类产品的文库的方法，筛选此类文库以产生显示针对taraget分子的结合特异性的实体的方法。 本发明进一步提供的是包含本发明的含半胱氨酸的产品的药物组合物。

    Method of increasing efficiency of directed evolution of a gene using phagemid
    67.
    发明授权
    Method of increasing efficiency of directed evolution of a gene using phagemid 失效
    使用噬菌粒增加基因定向进化效率的方法

    公开(公告)号：US6156509A

    公开(公告)日：2000-12-05

    申请号：US968627

    申请日：1997-11-12

    申请人： Volker Schellenberger

    发明人： Volker Schellenberger

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/86 ,  C12N15/01 ,  C12N15/10 ,  C12P21/02 ,  C12Q1/68

    CPC分类号： C12N15/01 ,  C12N15/102 ,  C12N9/86

    摘要： An improved method of mutanting DNA is provided comprising mutating a gene bearing phagemid in a host and infecting the host cell with a suitable helper phage to initiate packaging of the DNA into a phagemid. The packaged phagemid is then used to infect a second host cell. The method facilitates more efficient screening for genetic diversity. Also, the gene of interest is mutated and separable from the host cell which undergoes the mutational event reducing false positive results or other artifacts of general mutation techniques.

    摘要翻译： 提供突变DNA的改进方法包括在宿主中突变含有噬菌粒的基因并用合适的辅助噬菌体感染宿主细胞，以启动将DNA包装到噬菌粒中。 然后将包装的噬菌粒用于感染第二宿主细胞。 该方法有助于更有效地筛选遗传多样性。 此外，感兴趣的基因被突变并与经历突变事件的宿主细胞分离，减少假阳性结果或其他一般突变技术的伪像。