LABELED PEPTIDES, PROTEINS AND ANTIBODIES AND PROCESSES AND INTERMEDIATES USEFUL FOR THEIR PREPARATION
    64.
    发明申请
    LABELED PEPTIDES, PROTEINS AND ANTIBODIES AND PROCESSES AND INTERMEDIATES USEFUL FOR THEIR PREPARATION 失效
    标签肽,蛋白质和抗体及其制备和中间体用于制备

    公开(公告)号:US20070218502A1

    公开(公告)日:2007-09-20

    申请号:US11673909

    申请日:2007-02-12

    CPC分类号: C07K1/13 C07K1/1077

    摘要: The invention provides peptide synthons having protected functional groups for attachment of desired moieties (e.g. functional molecules or probes). Also provided are peptide conjugates prepared from such synthons, and synthon and conjugate preparation methods including procedures for identifying optimum probe attachment sites. Biosensors are provided having functional molecules that can locate and bind to specific biomolecules within living cells. Biosensors can detect chemical and physiological changes in those biomolecules as living cells are moving, metabolizing and reacting to its environment. Methods are included for detecting GTP activation of a Rho GTPase protein using polypeptide biosensors. When the biosensor binds GTP-activated Rho GTPase protein, an environmentally sensitive dye emits a signal of a different lifetime, intensity or wavelength than when not bound. New fluorophores whose fluorescence responds to environmental changes are also provided that have improved detection and attachment properties, and that can be used in living cells, or in vitro.

    摘要翻译: 本发明提供具有用于连接所需部分(例如功能分子或探针)的保护的官能团的肽合成子。 还提供了由这种合成子制备的肽缀合物,以及合成子和缀合物制备方法,包括鉴定最佳探针附着位点的程序。 生物传感器具有能够定位并结合活细胞内的特定生物分子的功能分子。 生物传感器可以检测生物分子中的化学和生理变化,因为活细胞正在移动,代谢并对其环境作出反应。 包括使用多肽生物传感器检测Rho GTPase蛋白的GTP活化的方法。 当生物传感器结合GTP激活的Rho GTPase蛋白质时,环境敏感的染料会发出与未结合不同的寿命,强度或波长的信号。 还提供了荧光对环境变化的响应的新荧光团,其具有改进的检测和附着性质,并且可用于活细胞或体外。

    Hand held pressure washer
    66.
    发明申请
    Hand held pressure washer 失效
    手持式压力清洗机

    公开(公告)号:US20070125878A1

    公开(公告)日:2007-06-07

    申请号:US11585503

    申请日:2006-10-24

    IPC分类号: B05B9/04

    摘要: A portable pressure washer includes a housing having a front end and a rear end defining a longitudinal axis. A handle has a first end extending from a rear portion of the housing and a second end terminating in a barrel. A first nozzle and a second aperture extend from the barrel. A fluid pump and a motor disposed are disposed within the housing, which includes a fluid inlet in fluid communication with the first nozzle and the second aperture.

    摘要翻译: 便携式压力清洗机包括具有限定纵向轴线的前端和后端的壳体。 手柄具有从壳体的后部延伸的第一端和终止于筒体的第二端。 第一喷嘴和第二孔从筒体延伸。 流体泵和设置的马达设置在壳体内,其包括与第一喷嘴和第二孔流体连通的流体入口。

    Multi-function power washer
    68.
    发明申请
    Multi-function power washer 失效
    多功能电动洗衣机

    公开(公告)号:US20060254008A1

    公开(公告)日:2006-11-16

    申请号:US11376610

    申请日:2006-03-14

    IPC分类号: A47L11/28

    摘要: A power washer is provided with a transport frame, a wash unit and a gun. The wash unit and the gun may be separated from the transport frame to be used in multiple modes. Possible modes include a walk-behind mode, a spray mode and hand wash modes. A quick connection is also provided to make it easier to disconnect and reconnect various components of the power washer.

    摘要翻译: 电动洗衣机设有运输框架,洗涤单元和枪。 洗涤单元和枪可以与运输框架分离以用于多种模式。 可能的模式包括步行模式,喷雾模式和手洗模式。 还提供快速连接,以便更容易地断开并重新连接电动洗衣机的各种部件。