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88 条记录 (耗时 0.034 秒)

    Process for Preparing L-amino Acids
    66.
    发明申请
    Process for Preparing L-amino Acids 审中-公开
    L-氨基酸的制备方法

    公开(公告)号：US20070122832A1

    公开(公告)日：2007-05-31

    申请号：US11615860

    申请日：2006-12-22

    申请人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P13/08 ,  C12N9/10 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1205 ,  C07K14/34 ,  C12N9/10 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

    摘要： The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70％ b）编码多肽的多核苷酸，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）的多核苷酸互补的多核苷酸， 或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少有citA基因 以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Enterobacteriaceae strains over-expressing the yfiD gene for the fermentative production of L-amino acids
    67.
    发明授权
    Enterobacteriaceae strains over-expressing the yfiD gene for the fermentative production of L-amino acids 有权
    肠杆菌科菌株过表达用于发酵生产L-氨基酸的yfiD基因

    公开(公告)号：US07211415B2

    公开(公告)日：2007-05-01

    申请号：US10817431

    申请日：2004-04-05

    申请人： Mechthild Rieping ,  Mike Farwick

    发明人： Mechthild Rieping ,  Mike Farwick

    IPC分类号： C12P13/04 ,  C12P13/08 ,  C12N1/20 ,  C12N1/21 ,  C12N15/74 ,  C12Q1/68 ,  C07H21/02

    CPC分类号： C12P13/08 ,  C12N15/52

    摘要： The present invention relates to a process for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, wherein a) the yfiD ORF and/or the pflB gene or nucleotide sequences coding for the gene products are overexpressed in the microorganisms producing the desired L-amino acid, and the microorganisms are cultured in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells; and b) the desired L-amino acid is isolated, in a manner such that constituents of the fermentation broth and/or the biomass in its entirety or in portions (>0 to 100%) either remain in the isolated product or are completely removed.

    摘要翻译： 本发明涉及通过发酵肠杆菌科的重组微生物生产L-氨基酸的方法，其中a）yfiD ORF和/或pflB基因或编码基因产物的核苷酸序列在微生物中过表达 产生所需的L-氨基酸，并且将微生物在培养基中培养，其中所需L-氨基酸在培养基中或细胞中富集; 和b）以如下方式分离所需的L-氨基酸，使得发酵液和/或生物量的整体或部分（> 0至100％）的组分保留在分离的产物中或完全除去 。

    Nucleotide sequences for encoding of the lysR2-gene
    69.
    发明授权
    Nucleotide sequences for encoding of the lysR2-gene 失效
    编码lysR2基因的核苷酸序列

    公开(公告)号：US07078502B2

    公开(公告)日：2006-07-18

    申请号：US09826909

    申请日：2001-04-06

    申请人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    发明人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，b）多核苷酸， 其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少lysR2基因存在于减毒 形式，以及使用多核苷酸序列作为杂交探针。