-
61.发明授权Self-calibrating gradient dilution in a constituent assay and gradient dilution apparatus performed in a thin film sample 有权在薄膜样品中进行的成分测定和梯度稀释装置中的自校准梯度稀释公开(公告)号：US08221985B2
公开(公告)日：2012-07-17
申请号：US12417333
申请日：2009-04-02
IPC分类号： G01N33/53
CPC分类号： G01N33/48 , G01N21/6428 , G01N33/5304 , G01N33/54373 , G01N33/54386
摘要： A method and apparatus for measuring antibody titers in a thin film sample in an automated system which does not require multiple dilutions. The system provides a simple method for creating an in-situ dilution within a sample analysis chamber without the use of any precision fluid-handling components, and further, to use the same principles to provide a wide range of sample dilutions within the chamber so as to obviate the need for additional dilution steps when dealing with samples possibly containing wide ranges of analyte concentrations.
摘要翻译： 一种在不需要多次稀释的自动化系统中测量薄膜样品中抗体滴度的方法和装置。 该系统提供了一种用于在样品分析室内创建原位稀释而不使用任何精密流体处理组件的简单方法，此外，使用相同的原理在腔室内提供范围广泛的样品稀释度，以便 以避免在处理可能含有大范围分析物浓度的样品时需要额外的稀释步骤。
-
62.发明申请公开(公告)号：US20120147357A1
公开(公告)日：2012-06-14
申请号：US13323438
申请日：2011-12-12
IPC分类号： G01N33/48
CPC分类号： G01N33/49 , G01N15/147 , G01N15/1475 , G01N2015/0076 , G01N2015/008 , G01N2015/0084 , G01N2015/0092 , G01N2015/1486
摘要： A method for enumerating platelets within a blood sample is provided. The method includes the steps of: 1) depositing the sample into a sample container having an analysis chamber adapted to quiescently hold the sample for analysis, and an amount of colorant that platelets absorb and which fluoresces upon exposure to one or more predetermined first wavelengths of light; 2) imaging at least a portion of the sample disposed in the analysis chamber, including producing image signals indicative of fluorescent emissions from the platelets illuminated by first wavelengths of light; 3) identifying the platelets using the image signals; and 4) enumerating individual platelets and clumped platelets within the sample using one or more of fluorescent emissions, area, shape, and granularity.
摘要翻译： 提供了一种用于列举血液样品中的血小板的方法。 该方法包括以下步骤：1）将样品沉积到具有分析室的样品容器中，所述分析室适于静态保持样品进行分析，以及一定量的着色剂，血小板被吸收并且在暴露于一个或多个预定的第一波长 光; 2）对设置在分析室中的样品的至少一部分进行成像，包括产生指示由第一波长的光照射的血小板的荧光发射的图像信号; 3）使用图像信号识别血小板; 和4）使用荧光发射，面积，形状和粒度中的一种或多种来列举样品中的各个血小板和聚集的血小板。
-
63.发明申请SYSTEMS AND METHODS FOR REDUCING EXPANSION OF FLUID CONTRAINING TUBES DURING CENTRIFUGATION 审中-公开在离心过程中减少流体对比管扩张的系统和方法公开(公告)号：US20110067488A1
公开(公告)日：2011-03-24
申请号：US12879984
申请日：2010-09-10
CPC分类号： B04B5/0414 , B01L3/50215 , B04B2005/0435
摘要： Systems and methods for determining the presence of a target material in a suspension are provided. In one embodiment, a system (100) for isolating a target material in suspension includes at least one tube and float system (106,108), an offset fluid (135) disposed with at least one chamber (101-104), and a centrifuge (109) for centrifugally processing the suspension disposed within the at least one tube and float system the at least one chamber.
摘要翻译： 提供了用于确定悬浮液中目标材料的存在的系统和方法。 在一个实施例中，用于分离悬架中的目标材料的系统（100）包括至少一个管和浮子系统（106,108），设置有至少一个室（101-104）的偏置流体（135）和离心机 109），用于离心处理设置在所述至少一个管内的所述悬浮液并且将所述至少一个室浮起。
-
64.发明申请DETECTION OF INFECTIOUS DISEASE IN A HUMAN OR ANIMAL BY MEASURING SPECIFIC PHAGOCYTOSIS IN A THIN FILM SAMPLE OF THEIR ANTICOAGULATED BLOOD 有权通过在其抗凝血液薄膜中测定特异性PHAGOCYTOSIS来检测人类或动物中的感染性疾病公开(公告)号：US20100255509A1
公开(公告)日：2010-10-07
申请号：US12417296
申请日：2009-04-02
IPC分类号： G01N33/53
CPC分类号： G01N33/56911 , C12Q1/04 , G01N15/1475 , G01N33/5094 , G01N33/5432 , G01N2015/008 , Y02A50/57
摘要： A method for performing a target analyte assay of a thin film anticoagulated blood sample, wherein the target analyte is the presence or absence of specific phagocytosis and/or binding of particles coated with a particular antigen or antigens by white blood cells present in the anticoagulated blood sample, wherein the particles are coated with the particular antigen or antigens, which antigens are similar or identical to antigens expressed by a defined pathogenic infectious agent of interest.
摘要翻译： 一种用于进行薄膜抗凝血液样品的目标分析物测定的方法，其中所述目标分析物是存在或不存在特定的吞噬作用和/或通过存在于抗凝血液中的白细胞与特定抗原或抗原结合的颗粒的结合 样品，其中所述颗粒用特定的抗原或抗原包被，所述抗原与所定义的感兴趣感染性感染物表达的抗原相似或相同。
-
65.发明申请METHOD OF DETECTING VERY LOW LEVELS OF ANALYTE WITHIN A THIN FILM FLUID SAMPLE CONTAINED IN A THIN THICKNESS CHAMBER 审中-公开检测在薄膜室中含有薄膜流体样品中分析物的极低水平的方法公开(公告)号：US20090258371A1
公开(公告)日：2009-10-15
申请号：US12417266
申请日：2009-04-02
IPC分类号： G01N33/53
CPC分类号： G01N33/588 , B82Y15/00 , G01N33/54366
摘要： A method and apparatus for the detection and quantification of very low levels of a target analyte using an imaging system is provided. In the case of some analytes such as certain hormones, for example TSH, their levels may be as low as several tens of thousands of molecules per micro liter. These extremely low levels can be measured by using the present invention to count the individual molecules of analyte. The invention also has the advantage of being a primary quantitative method, which is one which needs no standardization.
摘要翻译： 提供了使用成像系统检测和定量非常低水平的目标分析物的方法和装置。 在某些分析物如某些激素（例如TSH）的情况下，其水平可能低至每微升几万个分子。 通过使用本发明来计数分析物的各个分子，可以测量这些极低的水平。 本发明还具有作为不需要标准化的主要定量方法的优点。
-
66.发明申请METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING 有权使用荧光猝灭和/或漂白分析个体细胞或颗粒物的方法和装置公开(公告)号：US20090239257A1
公开(公告)日：2009-09-24
申请号：US12408448
申请日：2009-03-20
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence value for each the first constituents and second constituents using the image signals; g) determining an optical density value for each of the first constituents and second constituents, which optical density is a function of the colorant absorbed by the constituents, using the image signals; and h) identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
摘要翻译： 提供了一种用于分析血液样本的方法，其包括以下步骤：a）提供具有一个或多个第一成分和一个或多个第二成分的血液样品，所述第二成分与第一成分不同; b）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板和第二面板限定，两个面板都是透明的; c）将着色剂与所述样品混合，所述着色剂可操作以使得所述第一组分和第二组分在暴露于预定的第一波长的光时发荧光，并且所述着色剂可操作地吸收一个或多个预定的第二波长的光的光; d）以第一波长和第二波长照射含有第一组分和第二组分的样品的至少一部分; e）对样品的至少一部分进行成像，包括产生指示来自第一组分和第二组分的荧光发射的图像信号和第一组分和第二组分的光密度; f）使用图像信号确定每个第一成分和第二成分的荧光值; g）使用所述图像信号确定所述第一成分和第二成分中的每一个的光密度值，所述光密度值是由所述成分吸收的着色剂的函数; 和h）使用所确定的荧光和光密度值来识别第一组分和第二组分。
-
67.发明授权Method and apparatus for providing quality control in an instrument for medical analysis 有权用于在用于医学分析的仪器中提供质量控制的方法和装置公开(公告)号：US06748337B2
公开(公告)日：2004-06-08
申请号：US10021959
申请日：2001-12-14
IPC分类号： G01N3700
摘要： A method and apparatus for providing quality control in a medical analysis instrument is provided. The method includes the steps of: 1) sending one or more quality control specimens to an operator of the analytical instrument; 2) directly or indirectly communicating control data to the analytical instrument, wherein the control data relates acceptable standards to the analytical instrument; 3) analyzing the quality control specimen using the analytical instrument and thereby creating instrument analysis data; 4) evaluating the instrument analysis data using the control data to determine a functional status of the analytical instrument; 5) providing notice to the operator regarding the functional status of the analytical instrument; and 6) optionally preventing the reporting of patient data from the instrument if the functional status is unacceptable.
摘要翻译： 提供了一种用于在医疗分析仪器中提供质量控制的方法和装置。 该方法包括以下步骤：1）向分析仪器的操作者发送一个或多个质量控制样本; 2）直接或间接地将控制数据传达给分析仪器，其中控制数据将可接受标准与分析仪器相关联; 3）使用分析仪器分析质量控制标本，从而创建仪器分析数据; 4）使用控制数据评估仪器分析数据，以确定分析仪器的功能状态; 5）向运营人提供关于分析仪器功能状况的通知; 以及6）如果功能状态是不可接受的，则可选地防止从仪器报告患者数据。
-
68.发明授权Method for determining the effects of a growth-altering agent on a microbial colony 失效用于测定生长改变剂对微生物菌落的影响的方法公开(公告)号：US06472166B1
公开(公告)日：2002-10-29
申请号：US09506157
申请日：2000-02-17
IPC分类号： C12Q118
CPC分类号： C12Q1/18
摘要： A method for determining the effects of a growth-altering agent on one or more microbial colonies is provided. The method utilizes a growth-altering agent incorporated into at least a portion of a growth medium, and includes the steps of: (a) incubating the colony-forming units in a manner likely to cause the colony-forming units to replicate into microbial colonies; (b) inoculating the growth medium with an inoculum having one or more viable colony-forming units; (c) quantifying one or more characteristics of one or more individual microbial colonies exposed to the growth-altering agent; and (d) evaluating the quantified characteristics to determine the effects of the growth-altering agent on the individual microbial colonies.
摘要翻译： 提供了用于测定生长改变剂对一个或多个微生物菌落的影响的方法。 该方法利用掺入到生长培养基的至少一部分中的生长改变剂，并且包括以下步骤：（a）以可能导致集落形成单位复制到微生物菌落中的方式孵育菌落形成单位 ; （b）用具有一个或多个活菌落形成单位的接种物接种生长培养基; （c）定量暴露于生长改变剂的一个或多个单个微生物菌落的一个或多个特征; 和（d）评估量化特征以确定生长改变剂对各个微生物菌落的影响。
-
69.发明授权公开(公告)号：US06197523B1
公开(公告)日：2001-03-06
申请号：US08976886
申请日：1997-11-24
IPC分类号： G01N33574
CPC分类号： G01N15/042 , G01N33/56972 , G01N33/57484 , G01N2015/045 , Y10S436/813
摘要： A method for analyzing blood enables one to isolate, detect, enumerate and confirm under magnification the presence or absence of target cancer cells and/or hematologic progenitor cells which are known to circulate in blood. The analysis is performed in a sample of centrifuged anticoagulated whole blood. The analysis involves both morphometric and epitopic examination of the blood sample while the blood sample is disposed in a centrifuged blood sampling tube. The epitopic analysis of the presence or absence of cancer cells relies on the detection of epitopes which are known to present only on cancer cells; and the epitopic analysis of the presence or absence of hematologic progenitor cells relies on the detection of epitopes which are known to present only on hematologic progenitor cells. The targeted epitopes on the target cell types are epitopes which are also known to be absent on normal circulating blood cells; and the target cancer cell epitopes are epitopes which are known to be absent on target hematologic progenitor cells. Fluorophors with distinct emissions are coupled with antibodies which are directed against the targeted epitopes. The morphometric analysis is performed by staining the cells in the blood sample with an intracellular stain such as acridine orange which highlights the intracellular cell structure. Both the morphometric and epitopic analyses are preferably performed at or near the platelet layer of the expanded buffy coat in the centrifuged blood sample. The morphometric analysis and/or the epitopic analysis may be performed under magnification both visually and/or photometrically.
摘要翻译： 用于分析血液的方法使得能够在放大下分离，检测，枚举和确认已知在血液中循环的靶癌细胞和/或血液祖细胞的存在或不存在。 在离心的抗凝全血样品中进行分析。 该分析涉及血液样品的形态测定和表征检查，同时将血液样品置于离心取样管中。 癌细胞存在或不存在的代表性分析依赖于已知仅存在癌细胞的表位的检测; 并且血液祖细胞存在或不存在的表征分析依赖于已知仅存在血液祖细胞的表位的检测。 目标细胞类型上的靶向表位是已知在正常循环血细胞上不存在的表位; 并且目标癌细胞表位是已知在靶血液祖细胞上不存在的表位。 具有明显排放的荧光素与针对靶向表位的抗体相结合。 通过用细胞内染色剂如吖啶橙染色血液样品中的细胞进行形态测定分析，突出细胞内细胞结构。 形态测定和表征分析优选在离心血液样品中在扩张的血沉棕黄层的血小板层或其附近进行。 形态测定分析和/或表征分析可以在视觉上和/或光度下放大进行。
-
公开(公告)号：US5593848A
公开(公告)日：1997-01-14
申请号：US335310
申请日：1994-11-07
IPC分类号： G01N33/48 , A61K9/127 , G01N33/532 , G01N33/534 , G01N33/537 , G01N33/538 , G01N33/544 , G01N33/533 , G01N33/536
CPC分类号： G01N33/5375 , G01N33/538 , Y10S436/829
摘要： An improved assay of target components in a sample utilizes specific gravity-altering particles which are attached to the target components by specific antibodies. The attached specific gravity-altering particles are preferably liposomes which will buoy or sink the targets to a common level in the specimen sample when the latter has been centrifuged in a transparent tube. The liposomes can provide an accentuated and more pronounced indication of the presence of the targets in the sample due to their ability to contain many multiples of fluorescent or non-fluorescent dye molecules with minimal steric interference with the attached antibodies' binding ability.
摘要翻译： 样品中目标组分的改进测定利用通过特异性抗体附着于靶组分的比重改变颗粒。 附着的比重改变颗粒优选是脂质体，当将后者已经在透明管中离心时，其将标本浮标或将目标物吸收到标本样品中的共同水平。 脂质体可以提供样品中靶标存在的突出和更显着的指示，因为它们具有包含许多倍数的荧光或非荧光染料分子的能力，其具有与所附抗体结合能力的最小空间干扰。
-
-
-
-
-
-
-
-
-
搜索结果
209 条记录 (耗时 0.051 秒)
