-
61.发明申请公开(公告)号：US20110236900A1
公开(公告)日：2011-09-29
申请号：US13130993
申请日：2009-11-27
CPC分类号： C07K14/195 , G01N33/5308 , G01N2333/32
摘要： A method for determining the presence or absence of a mutation on the basis of the presence or absence of amplification with high reliability is provided. A target sequence including a target site contained in a sample nucleic acid is amplified using a primer that can hybridize to a region including the target site contained in the sample nucleic acid in the presence of a novel MutS having an amino acid sequence of SEQ ID NO: 2, and then the presence or absence of a mutation at the target site is determined on the basis of the presence or absence of amplification. The novel MutS binds more specifically to a mismatched base pair than to a fully-matched base pair, whereby an extension reaction caused by a mismatch-binding primer is suppressed. Thus, according to the present invention, the presence or absence of a mutation can be determined with high reliability.
摘要翻译： 提供了一种基于高可靠性的扩增的存在或不存在来确定突变的存在或不存在的方法。 包含样品核酸中包含的靶位点的靶序列使用可以在含有氨基酸序列SEQ ID NO的新型MutS的存在下与包含在样品核酸中的靶位点的区域杂交的引物进行扩增 ：2，然后基于扩增的存在或不存在来确定靶位点处的突变的存在或不存在。 新的MutS更具体地结合失配的碱基对而不是完全匹配的碱基对，由此抑制由错配结合引物引起的延伸反应。 因此，根据本发明，可以高可靠性确定突变的存在或不存在。
-
62.发明申请公开(公告)号：US20080020474A1
公开(公告)日：2008-01-24
申请号：US10594770
申请日：2005-03-29
CPC分类号： G01N33/54373 , H01J49/162 , Y10T436/24 , Y10T436/25
摘要： A method of analyzing a biosample that enables substantial shortening of time required for analysis, further enabling obtaining highly reliable results through means for avoiding sway of analytical results depending on observers, and that enables one-time analysis of a multiplicity of genes, etc. on a single biosample to thereby enhance workload and time efficiencies, and that enables analysis of multiple genes, etc. under conditions completely free from any difference in background attributed to biosamples. There is provided a method comprising irradiating a biosample as an analyte with ultra-short pulse laser beams to thereby effect an ablation thereof so that molecules contained in the biosample are atomized into constituting elements, ionizing the constituting elements resulting from the atomization and analyzing the ionized constituting elements to thereby analyze analytical-target molecules of the biosample.
摘要翻译： 一种分析生物样本的方法，其能够显着缩短分析所需的时间，进一步通过取决于观察者的分析结果的摆动的手段获得高度可靠的结果，并且使得能够对多个基因等进行一次性分析 一个单一的生物样品，从而提高工作量和时间效率，并且可以在完全没有归因于生物样本的背景差异的条件下分析多个基因等。 提供了一种方法，包括用超短脉冲激光束照射作为分析物的生物样品，从而进行消融，使得生物样品中包含的分子被雾化成构成元素，电离由雾化产生的构成元素并分析离子化 构成元件，从而分析生物样品的分析目标分子。
-
63.发明申请Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same 有权扩增核酸的方法和使用该方法检测突变核酸的方法公开(公告)号：US20070190531A1
公开(公告)日：2007-08-16
申请号：US10583706
申请日：2004-12-24
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q2537/163 , C12Q2531/113 , C12Q2521/514 , C12Q2525/301 , C12Q2525/161 , C12Q2537/125 , C12Q2522/101
摘要： A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) located in the 3′ end portion of the target nucleic acid sequence. The first primer also contains, on the 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5′ side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3′ end portion, a sequence (Cc′) that hybridizes to a sequence (C) located in the 3′ end portion of a complementary sequence to the target nucleic acid sequence. The second primer also contains, on the 5′ side of the sequence (Cc′), a folded sequence (D-Dc′) that contains, on the same strand, two nucleic acid sequences that hybridize to each other.
摘要翻译： 引物组，其允许靶核酸被特异性和有效地扩增。 本发明的引物组包括允许靶核酸序列扩增的至少两个引物。 引物组中包含的第一引物在其3'末端含有与位于靶核酸序列3'端部分的序列（A）杂交的序列（Ac'）。 第一引物还在序列的5'侧包含与5'侧存在的序列（B）互补序列（Bc）杂交的序列（B'），相对于 涉及靶核酸序列中的序列（A）。 包括在引物组中的第二引物在其3'末端包含与位于与靶核酸序列的互补序列的3'端部分的序列（C）杂交的序列（Cc'）。 第二引物还包含在序列的5'侧（Cc'）上的折叠序列（D-Dc'），其在同一条链上含有彼此杂交的两个核酸序列。
-
公开(公告)号：US20070178482A1
公开(公告)日：2007-08-02
申请号：US11462939
申请日：2006-08-07
申请人： Alexander Lezhava , Yuko Shibata , Matthias Harbers , Toshizo Hayashi , Yoshihide Hayashizaki , Piero Carninci
发明人： Alexander Lezhava , Yuko Shibata , Matthias Harbers , Toshizo Hayashi , Yoshihide Hayashizaki , Piero Carninci
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q2522/101 , C12Q2521/301 , C12Q2521/325 , C12Q2527/143
摘要： The invention is a method and a kit for preparing single-stranded DNA from double-stranded DNA and the purification of single-stranded DNA derived from double-stranded DNA. A single-stranded-DNA binding substance is used in combination with a double-strand-specific endonuclease for the removal of undesired double-stranded DNA from a single-stranded DNA preparation and for other related purposes.
摘要翻译： 本发明是用于从双链DNA制备单链DNA并纯化由双链DNA衍生的单链DNA的方法和试剂盒。 单链DNA结合物质与双链特异性内切核酸酶组合使用，用于从单链DNA制备物中除去不想要的双链DNA并用于其它相关目的。
-
65.发明申请Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes 审中-公开其上固定有酶的载体，印刷材料和试剂盒，制备载体的方法，酶的储存方法和恢复酶的方法公开(公告)号：US20070117094A1
公开(公告)日：2007-05-24
申请号：US10574165
申请日：2004-09-29
CPC分类号： C12N9/1252 , C12N9/96 , C12N11/12
摘要： The invention enables enzymes to be stored in a simple manner. Disclosed are a support having an enzyme and a protecting agent for the enzyme fixed thereon; a printed material and a reagent kit comprising the support; a method for preparing the support; a method for restoring the enzyme fixed on the support; and a method for storing an enzyme in the form of being fixed on a support as a mixture with a protecting agent for the enzyme.
摘要翻译： 本发明能够以简单的方式储存酶。 公开了具有固定在其上的酶和保护剂的载体; 印刷材料和包含所述载体的试剂盒; 一种制备载体的方法; 用于恢复固定在载体上的酶的方法; 以及以与酶的保护剂作为混合物固定在载体上的形式的酶的储存方法。
-
66.发明授权公开(公告)号：US07195698B2
公开(公告)日：2007-03-27
申请号：US10318124
申请日：2002-12-13
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
-
公开(公告)号：US20070042363A1
公开(公告)日：2007-02-22
申请号：US10509727
申请日：2003-01-22
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C07K14/705
CPC分类号： C07K14/47
摘要： A novel protein which binds with tumor necrosis factor receptor associated factor 2, TRAF2, as well as a nucleic acid coding for the protein is disclosed. From a mouse cDNA library, a cDNA coding for a novel protein which binds with TRAF2 was discovered by mammalian two-hybrid assay, the cDNA was sequenced, the protein encoded by the cDNA was produced, and the fact that the protein binds with TRAF2 was experimentally confirmed.
摘要翻译： 公开了与肿瘤坏死因子受体相关因子2，TRAF2结合的新型蛋白质以及编码该蛋白质的核酸。 从小鼠cDNA文库中，通过哺乳动物双杂交测定发现编码与TRAF2结合的新蛋白质的cDNA，对cDNA进行测序，产生由cDNA编码的蛋白质，并且蛋白质与TRAF2结合的事实为 实验证实。
-
公开(公告)号：US07122382B1
公开(公告)日：2006-10-17
申请号：US09959768
申请日：2000-04-28
IPC分类号： G01N33/543 , G01N33/53
CPC分类号： C40B30/04 , C07K1/13 , G01N33/6803 , G01N33/6842 , G01N33/6845 , Y10S436/824
摘要： A method for detecting proteins under mutual interaction which comprises mixing a protein having a label for detection synthesized by a cell-free protein synthesis method with another protein having a modification for separation, or synthesizing a protein having a label for detection and another protein having a modification for separation in a single system by the cell-free protein synthesis method; separating a pair of proteins formed by the interaction between these proteins with the use of the modification for separation as described above; and distinguishing these proteins with the use of the label for detection as described above. A screening method using this method.
摘要翻译： 一种相互作用下蛋白质检测方法，其特征在于，将具有无细胞蛋白质合成方法合成检测标记的蛋白质与具有分离修饰的另一种蛋白质混合，或合成具有检测标记物的蛋白质和具有 通过无细胞蛋白质合成方法在单一系统中进行分离修饰; 通过使用如上所述的分离修饰来分离由这些蛋白质之间的相互作用形成的一对蛋白质; 并使用如上所述的用于检测的标记区分这些蛋白质。 使用该方法的筛选方法。
-
公开(公告)号：US20060194207A1
公开(公告)日：2006-08-31
申请号：US10566134
申请日：2004-07-29
CPC分类号： B01L3/502 , B01L3/5082 , B01L2200/0621 , B01L2300/0672 , B01L2300/0832 , B01L2300/087 , B01L2400/0677 , B01L2400/0683
摘要： The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for detecting a target nucleic acid from a sample, comprising at least a first compartment which contains an extraction reagent composition for extracting nucleic acids from said sample, a second compartment which contains an amplification reagent composition for amplifying the target nucleic acid, a separating means for separating the first and second compartments, and an aperture which enables to introduce said sample into only said first compartment. The separating means breaks the separation of the first and second compartments by physical energy supplied from the outside of the reactor, and thereby makes it possible to mix the extraction reagent composition in the first compartment and the amplification reagent composition in the second compartment.
摘要翻译： 本发明提供一种用于在单一容器中进行提取核酸和扩增核酸的步骤的反应器，所述步骤的步骤通常彼此不同。 根据本发明的反应器是用于从样品中检测靶核酸的反应器，其包含至少第一隔室，其包含用于从所述样品中提取核酸的提取试剂组合物，第二室含有用于 扩增目标核酸，用于分离第一和第二隔室的分离装置，以及能够将所述样品引入所述第一隔室的孔。 分离装置通过从反应器外部提供的物理能量破坏第一和第二隔室的分离，从而使得可以将第一隔室中的提取试剂组合物和第二隔室中的扩增试剂组合物混合。
-
公开(公告)号：US20060108538A1
公开(公告)日：2006-05-25
申请号：US10525433
申请日：2003-08-25
IPC分类号： H01J27/24
CPC分类号： H01J49/161 , G01N21/718 , G01N33/68 , G01N33/6803 , G01N33/6851 , H01J49/40
摘要： A method of analyzing a protein using laser ablation which comprises, in order to simultaneously atomize the atoms constituting the protein to be analyzed and ionize the same using a single laser source, irradiating the protein with laser beams and thus ablating the protein to thereby atomize the protein into the constituting elements and ionize the thus atomized constituting elements, and then analyzing the constituting elements thus ionized. The laser beams for ablating the subject protein are ultra-short pulse laser beams with which a chip having the protein fixed thereon is irradiated. Thus, the protein fixed on the chip is ablated with the ultra-short pulse laser beams so that the protein is atomized into the constituting elements and, at the same time, ionized followed by the analysis of the constituting elements thus ionized.
摘要翻译： 一种使用激光烧蚀分析蛋白质的方法，其包括为了使用单一激光源同时雾化构成待分析蛋白质的原子并将其电离，同时用激光束照射蛋白质，从而消除蛋白质从而雾化 蛋白质成为构成元素，并将这样雾化的构成元素电离，然后分析由此电离的构成元素。 用于烧蚀受试蛋白的激光束是照射固定有蛋白质的芯片的超短脉冲激光束。 因此，利用超短脉冲激光束将固定在芯片上的蛋白质消融，使蛋白质雾化成为构成元素，同时电离，然后对由此电离的构成元素进行分析。
-
-
-
-
-
-
-
-
-
搜索结果
78 条记录 (耗时 0.032 秒)