公开(公告)号：US20180003634A1

公开(公告)日：2018-01-04

申请号：US15638902

申请日：2017-06-30

申请人： Shenzhen Mindray Bio-Medical Electronics., Ltd.

发明人： Ye BO ,  Qi HUAN ,  Qian CHENG

IPC分类号： G01N21/64 ,  G01N33/49

CPC分类号： G01N21/64 ,  G01N15/1459 ,  G01N33/49 ,  G01N2015/0076

摘要： Disclosed are nucleated red blood cell warning devices and methods, and flow cytometers using the same. The devices, methods and flow cytometers can warn whether nucleated red blood cells exist in a blood sample. The warning device may obtain forward-scattered light information, side-scattered light information and fluorescence information when cells in the blood sample pass through a detection region of the flow cytometer. The warning device may generate a side-scattered light-fluorescence dot plot, so as to classify leucocytes into four groups, and may generate a forward-scattered light-fluorescence dot plot, where the forward-scattered light-fluorescence dot plot can include a leucocyte group region. The warning device may obtain a predetermined feature region located at the left side of the leucocyte group region, perform statistics on the amount of characterization cells in the predetermined feature region, and provide a warning when the number of the characterization cells exceeds a threshold value.

公开(公告)号：US20170315046A1

公开(公告)日：2017-11-02

申请号：US15163541

申请日：2016-05-24

申请人： SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD.

发明人： Xiansuan DU ,  Yong DAI

IPC分类号： G01N15/14 ,  G01N21/64 ,  G01N21/47 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N21/4738 ,  G01N21/6428 ,  G01N2015/0076 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2201/06113 ,  G01N2201/124

摘要： A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.

公开(公告)号：US20150125900A1

公开(公告)日：2015-05-07

申请号：US14595341

申请日：2015-01-13

申请人： SYSMEX CORPORATION

发明人： Takaaki NAGAI ,  Noriyuki NARISADA ,  Daigo FUKUMA

IPC分类号： G01N33/50 ,  G01N15/14 ,  G01N33/72

CPC分类号： G01N33/5091 ,  G01N15/12 ,  G01N15/1459 ,  G01N33/5094 ,  G01N33/721 ,  G01N33/726 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/1486 ,  Y10T436/11 ,  Y10T436/113332

摘要： A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.

公开(公告)号：US08906309B2

公开(公告)日：2014-12-09

申请号：US12767611

申请日：2010-04-26

申请人： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

发明人： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

IPC分类号： G01N33/00 ,  G01N31/00 ,  G01N15/00 ,  G01N15/14 ,  G01N33/49

CPC分类号： G01N15/1404 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/492 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2800/22 ,  Y10T436/101666

摘要： A method for identifying, analyzing, and quantifying the cellular components of whole blood by use of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by use of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

摘要翻译： 一种通过使用自动血液分析仪来鉴定，分析和定量全血细胞成分的方法，以及每个细胞散射，吸收和荧光发射的光的检测。 更具体地，上述方法包括通过使用波长范围为约400nm至约450nm的光源和多次流入光学测量和染色来识别，分析和定量全血的细胞组分，并且不需要 裂解红细胞。

公开(公告)号：US08721964B2

公开(公告)日：2014-05-13

申请号：US12727938

申请日：2010-03-19

申请人： Yoichi Nakamura ,  Hideki Hirayama ,  Hideaki Matsumoto ,  Keiko Moriyama

发明人： Yoichi Nakamura ,  Hideki Hirayama ,  Hideaki Matsumoto ,  Keiko Moriyama

IPC分类号： G01N33/00

CPC分类号： G01N15/147 ,  G01N2015/0076 ,  G01N2015/0092

摘要： An animal blood cell measuring apparatus comprising: a specimen preparation section for preparing a measurement specimen from blood of an animal; a characteristic information obtaining section for obtaining characteristic information indicating a characteristic of the measurement specimen, from the measurement specimen prepared by the specimen preparation section; and a controller configured for performing operations comprising: (a) classifying aggregate reticulocytes contained in the blood from other blood cells, based on the characteristic information obtained by the characteristic information obtaining section; and (b) outputting information regarding a number of the classified aggregate reticulocytes.

摘要翻译： 一种动物血细胞测定装置，其特征在于，具备：动物的血液制作测定标本的试样准备部; 特征信息获取部分，用于从由样本制备部分准备的测量样本获得指示测量样本的特征的特性信息; 以及控制器，被配置为执行操作，所述操作包括：（a）基于由所述特征信息获取部获得的特征信息，将来自其他血细胞的血液中包含的网状细胞集合进行分类; 和（b）输出关于分类的网状细胞总数的信息。

公开(公告)号：US20130023007A1

公开(公告)日：2013-01-24

申请号：US13549211

申请日：2012-07-13

申请人： Michael Zahniser ,  Russell Zahniser

发明人： Michael Zahniser ,  Russell Zahniser

IPC分类号： G01N21/25 ,  C12M1/42

CPC分类号： G01N15/1475 ,  G01N15/1429 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1006 ,  G01N2015/1465 ,  G01N2015/1472 ,  G06K9/0014 ,  G06K9/46 ,  G06K9/4652 ,  G06K9/52 ,  G06K2009/4666 ,  G06T7/0012 ,  G06T7/62 ,  G06T2207/10024 ,  G06T2207/30004 ,  G06T2207/30024 ,  G06T2207/30242 ,  H04N7/18

摘要： Methods and systems for identifying reticulocytes in a blood sample deposited on a substrate include: illuminating the sample with incident light at two different wavelengths, obtaining a two-dimensional image of the sample corresponding to a first one of the wavelengths, and obtaining a two-dimensional image of the sample corresponding to a second one of the wavelengths; analyzing the images to identify a set of representative red blood cells; determining an area of each of the red blood cells in the set; determining a color value of each of the red blood cells in the set; and, for each one of the red blood cells in the set, identifying the red blood cell as a reticulocyte if the area of the red blood cell exceeds an area cutoff value and the color value of the red blood cell is less than a color cutoff value.

公开(公告)号：US08339585B2

公开(公告)日：2012-12-25

申请号：US13312832

申请日：2011-12-06

申请人： Mark A. Wells ,  Carlos A. Perez ,  Jose M. Cano

发明人： Mark A. Wells ,  Carlos A. Perez ,  Jose M. Cano

IPC分类号： G01N33/48 ,  G01N21/00

CPC分类号： G01N21/532 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084

摘要： Transducer modules for use in a blood analysis instrument and methods for analyzing a blood sample. The transducer modules presented generally include a light source, a focus-alignment system, a flow cell, and a light scatter detection system. Electrodes within the flow cell allow for the measurement of the DC impedance and RF conductivity of cells passing through a cell-interrogation zone in the flow cell. Light scatter from the cells passing through the cell-interrogation zone is measured by the light scatter detection system. The light scatter detection system measures the light scatter parameters of upper median light scatter, lower median angle light scatter, low angle light scatter, and axial light loss. The presented methods for analyzing a blood sample generally include aspirating a whole blood sample into a blood analysis instrument, preparing the blood sample for analysis, passing the blood sample through a flow cell in a transducer system, and measuring axial light loss, multiple angles of light scatter, DC impedance and/or RF conductivity.

摘要翻译： 用于血液分析仪器的传感器模块和用于分析血液样本的方法。 所呈现的换能器模块通常包括光源，聚焦对准系统，流动池和光散射检测系统。 流动池内的电极允许测量通过流动池中的细胞询问区的细胞的DC阻抗和RF电导率。 通过细胞询问区的细胞的光散射通过光散射检测系统测量。 光散射检测系统测量高中值光散射，较低中角度光散射，低角度光散射和轴向光损失的光散射参数。 提出的分析血液样本的方法通常包括将全血样品吸入血液分析仪器，准备用于分析的血液样品，使血液样品通过换能器系统中的流动池，并测量轴向光损失，多个角度 光散射，DC阻抗和/或RF电导率。

公开(公告)号：US08154713B2

公开(公告)日：2012-04-10

申请号：US12550626

申请日：2009-08-31

申请人： Ramon Simon-Lopez

发明人： Ramon Simon-Lopez

IPC分类号： G01N33/48

CPC分类号： G01N33/90 ,  G01N15/12 ,  G01N33/721 ,  G01N2015/0069 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1062 ,  G01N2015/1093 ,  Y10T436/11

摘要： New diagnostic parameters or indexes for detection of absolute iron deficiency, latent iron deficiency, functional iron deficiency, or latent functional iron deficiency have been disclosed. The parameters include a RBC size factor, RSf1 defined by the formula of (MCV×MRV)1/2, or RSf2 defined by the formula of (MCV×MRV)/100, a volume-hemoglobin factor (VHf) defined by the formula of (MCV×Hgb)/100, and a volume-hemoglobin/distribution factor (VHDWf) defined by the formula of (MCV×Hgb)/(RDW×10). Further disclosed are the methods of using these parameters for detection of absolute iron deficiency, latent iron deficiency, functional iron deficiency, or latent functional iron deficiency. Also disclosed is a method of using RSf for detection of hemochromatosis.

摘要翻译： 已经公开了用于检测绝对铁缺乏，潜在铁缺乏，功能性铁缺乏或潜在功能性铁缺乏症的新的诊断参数或指标。 参数包括RBC尺寸因子，由（MCV×MRV）1/2的公式定义的RSf1或由（MCV×MRV）/ 100的式定义的RSf2，由式（5）定义的体积 - 血红蛋白因子（VHf） （MCV×Hgb）/ 100，和由（MCV×Hgb）/（RDW×10）式定义的体积血红蛋白/分布因子（VHDWf）。 还公开了使用这些参数来检测绝对铁缺乏，潜铁缺乏，功能性铁缺乏或潜在功能缺铁的方法。 还公开了使用RSf检测血色素沉着症的方法。

公开(公告)号：US08094299B2

公开(公告)日：2012-01-10

申请号：US12178817

申请日：2008-07-24

申请人： Mark A. Wells ,  Carlos A. Perez ,  Jose M. Cano

发明人： Mark A. Wells ,  Carlos A. Perez ,  Jose M. Cano

IPC分类号： G01N21/01

CPC分类号： G01N21/532 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084

摘要： Transducer modules for use in a blood analysis instrument and methods for analyzing a blood sample. The transducer modules presented generally include a light source, a focus-alignment system, a flow cell, and a light scatter detection system. Electrodes within the flow cell allow for the measurement of the DC impedance and RF conductivity of cells passing through a cell-interrogation zone in the flow cell. Light scatter from the cells passing through the cell-interrogation zone is measured by the light scatter detection system. The light scatter detection system measures the light scatter parameters of upper median light scatter, lower median angle light scatter, low angle light scatter, and axial light loss. The presented methods for analyzing a blood sample generally include aspirating a whole blood sample into a blood analysis instrument, preparing the blood sample for analysis, passing the blood sample through a flow cell in a transducer system, and measuring axial light loss, multiple angles of light scatter, DC impedance and/or RF conductivity.

公开(公告)号：US07962292B2

公开(公告)日：2011-06-14

申请号：US12012431

申请日：2008-01-31

申请人： Naohiko Matsuo ,  Hiroyuki Fujino ,  Mitsuyo Koya ,  Susumu Hoshiko

发明人： Naohiko Matsuo ,  Hiroyuki Fujino ,  Mitsuyo Koya ,  Susumu Hoshiko

IPC分类号： G01N31/00

CPC分类号： G01N35/00594 ,  G01N15/12 ,  G01N15/1459 ,  G01N21/272 ,  G01N21/82 ,  G01N33/4905 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/1486

摘要： A sample analyzer optically measures reaction of a sample mixed with reagent, and obtains optical information therefrom; generates a reaction curve representing change in the optical information over time; determines a first area prior to an evaluation target time (t0) and a second area after the evaluation target time (t0) wherein the first and second areas are formed between a baseline which is parallel to the time axis and a reaction curve from a first time (t1) prior to the optional evaluation target time (t0) to a second time (t2) after the evaluation target time, and determines the reaction end point based on the first and second areas; and obtains a characteristic of a sample based on the determined reaction end point, is disclosed. a sample analyzing method is also disclosed.

摘要翻译： 样品分析仪光学测量与试剂混合的样品的反应，并从中获得光学信息; 产生表示光信息随时间变化的反应曲线; 确定在评估目标时间（t0）之前的第一区域和在评估目标时间（t0）之后的第二区域，其中在与时间轴平行的基线和来自第一个时间轴的反应曲线之间形成第一和第二区域 在评估目标时间之后的可选评估目标时间（t0）到第二时间（t2）之前的时间（t1），并且基于第一和第二区域确定反应结束点; 并且基于所确定的反应终点获得样品的特性。 还公开了一种样品分析方法。