公开(公告)号：US20110021376A1

公开(公告)日：2011-01-27

申请号：US12895064

申请日：2010-09-30

申请人： Yehudit Amor ,  Ofer Markman ,  Mirit Kolog Gulko ,  Albena Samokovlisky ,  Fredi Kleinman ,  Tal Alergand ,  Rakefet Rosenfeld ,  Ruth Maya ,  Sabina Rebe ,  Idil Kelson Kasuto ,  Haim Bangio

发明人： Yehudit Amor ,  Ofer Markman ,  Mirit Kolog Gulko ,  Albena Samokovlisky ,  Fredi Kleinman ,  Tal Alergand ,  Rakefet Rosenfeld ,  Ruth Maya ,  Sabina Rebe ,  Idil Kelson Kasuto ,  Haim Bangio

IPC分类号： C40B30/04

CPC分类号： G01N33/5308 ,  G01N2333/42 ,  G01N2333/924 ,  G01N2400/02

摘要： The invention relates generally the structural analysis of glycomolecule-containing macromolecules, such as those that occur either attached to proteins (proteoglycans, glycoproteins), lipids, or as free saccharides.

摘要翻译： 本发明一般涉及含糖分子的大分子的结构分析，例如与蛋白质（蛋白聚糖，糖蛋白），脂质或游离糖连接的那些。

公开(公告)号：US20090148865A1

公开(公告)日：2009-06-11

申请号：US12269273

申请日：2008-11-12

申请人： Ofer Markman

发明人： Ofer Markman

IPC分类号： G01N33/53 ,  G01N33/52 ,  C40B40/00 ,  C40B30/02

CPC分类号： G01N33/66 ,  C12Q1/34 ,  G01N33/5308 ,  G01N2333/42 ,  G01N2333/924 ,  G01N2400/00 ,  G01N2400/02 ,  G01N2400/10 ,  Y10T436/143333

摘要： The invention provides a method for the structural analysis of a saccharide, comprising: a) providing on a surface a plurality of essentially sequence- and/or site-specific binding agents; b) contacting said surface with a saccharide to be analyzed, or with a mixture comprising a plurality of fragments of said saccharide; c) washing or otherwise removing unbound saccharide or saccharide fragments; d) adding to the surface obtained in step c) an essentially sequence- and/or site-specific marker, or a mixture of essentially sequence- and/or site-specific markers; e) acquiring one or more images of the markers that are bound to said surface; and f) deriving information related to the identity of the saccharide being analyzed from said image.

摘要翻译： 本发明提供了一种用于糖的结构分析的方法，包括：a）在表面上提供多个基本上序列和/或位点特异性结合剂; b）使所述表面与待分析的糖接触，或与包含所述糖的多个片段的混合物接触; c）洗涤或以其他方式去除未结合的糖或糖片段; d）加入步骤c）中获得的表面基本上是序列和/或位点特异性标记，或基本上序列和/或位点特异性标记的混合物; e）获取结合到所述表面的标记的一个或多个图像; 以及f）从所述图像导出与正在分析的糖的身份有关的信息。

公开(公告)号：US20080085838A1

公开(公告)日：2008-04-10

申请号：US11896717

申请日：2007-09-05

申请人： Avinoam Dukler ,  Nir Dotan

发明人： Avinoam Dukler ,  Nir Dotan

IPC分类号： C40B30/04

CPC分类号： C40B30/04 ,  C07B2200/11 ,  C07H15/02 ,  C07H15/203 ,  C07K14/59 ,  G01N33/5308 ,  G01N33/543 ,  G01N33/66 ,  G01N33/6842 ,  G01N33/6845 ,  G01N2333/42 ,  G01N2400/02 ,  G01N2400/10

摘要： A combinatorial complex carbohydrate library is provided and including a plurality of addressable complex carbohydrate structures.

摘要翻译： 提供组合复合物碳水化合物文库并且包括多个可寻址的复合碳水化合物结构。

公开(公告)号：US20050221380A1

公开(公告)日：2005-10-06

申请号：US10499297

申请日：2002-12-20

申请人： Johannes Muthing ,  Jasna Peter-Katalinic ,  Martin Langer ,  Babette Mockel ,  Jurgen Eck

发明人： Johannes Muthing ,  Jasna Peter-Katalinic ,  Martin Langer ,  Babette Mockel ,  Jurgen Eck

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K16/28 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/48 ,  G01N33/566 ,  G01N33/573 ,  G01N33/574 ,  G01N33/577 ,  G01N33/66 ,  A61K38/16

CPC分类号： C12Q1/48 ,  C07K16/18 ,  G01N33/566 ,  G01N33/66 ,  G01N2333/42 ,  G01N2400/00 ,  G01N2800/52

摘要： A method for the determination of the responsiveness of an individual to mistletoe lectin or to (an) mistletoe lectin single chain(s), wherein the expression of a membrane-bound receptor for mistletoe lectin is characteristic of a corresponding responsiveness.

摘要翻译： 用于测定个体对槲寄生凝集素或（一种）槲寄生凝集素单链的响应性的方法，其中槲寄生凝集素的膜结合受体的表达是相应的响应性的特征。

公开(公告)号：US20020150878A1

公开(公告)日：2002-10-17

申请号：US10051653

申请日：2002-01-17

发明人： David Henry Small ,  Lisa Fodero

IPC分类号： C12Q001/00 ,  G01N033/574

CPC分类号： G01N33/6896 ,  G01N2333/42 ,  G01N2333/918 ,  G01N2400/00 ,  G01N2800/2821 ,  G01N2800/2828

摘要： The invention provides a method for the diagnosis of dementia and transmissible spongiform encephalopathies in a subject by detecting the levels of glycoproteins that bind wheat germ agglutinin. The invention also provides a method for diagnosis of dementia and transmissible spongiform encephalopathies in a subject by comparing the levels of glycoproteins that bind wheat germ agglutinin with the glycosylation patterns of biomarkers, acetylcholinesterase and butyrylcholinesterase.

摘要翻译： 本发明提供一种通过检测结合小麦胚芽凝集素的糖蛋白的水平来诊断受试者的痴呆和传染性海绵状脑病的方法。 本发明还提供了一种用于通过比较结合小麦胚芽凝集素的糖蛋白与生物标志物，乙酰胆碱酯酶和丁酰胆碱酯酶的糖基化模式的水平来诊断受试者的痴呆和传染性海绵状脑病的方法。

公开(公告)号：US5695949A

公开(公告)日：1997-12-09

申请号：US418495

申请日：1995-04-07

申请人： Robert S. Galen ,  John F. Burd ,  Talei Hoblitzell ,  Gebhard Neyer

发明人： Robert S. Galen ,  John F. Burd ,  Talei Hoblitzell ,  Gebhard Neyer

IPC分类号： C12Q1/34 ,  C12Q1/54 ,  G01N33/52 ,  G01N33/66 ,  G01N31/22

CPC分类号： C12Q1/34 ,  C12Q1/54 ,  G01N33/526 ,  G01N33/66 ,  C12Q2326/12 ,  C12Q2326/14 ,  C12Q2326/92 ,  G01N21/645 ,  G01N2333/42 ,  Y10S435/97 ,  Y10S436/828 ,  Y10T436/144444

摘要： The present invention is directed to a single test system and method for determining the integrated glycemic condition of a subject by measuring the concentration of glucose and the level of protein-bound glucose in a subject's body fluid, such as whole blood. The glucose concentration is indicative of the subject's immediate glycemic condition, whereas the protein-bound glucose concentration is indicative of either intermediate or long-term glycemic condition. Optionally, other analytes indicative of glycemic condition, such as ketone bodies or fatty acid derivatives, can also be measured. The present invention also provides a method of diagnosing diabetes. The invention additionally provides a method for analyzing the concentration of fructosamine in less than or equal to five minutes without the use of a reaction accelerator.

摘要翻译： 本发明涉及用于通过测量受试者体液（例如全血）中葡萄糖浓度和蛋白质结合葡萄糖水平来测定受试者的综合血糖状况的单一测试系统和方法。 葡萄糖浓度表示受试者的立即血糖状况，而蛋白质结合的葡萄糖浓度则指示中度或长期血糖状态。 任选地，还可以测量指示血糖状况的其它分析物，例如酮体或脂肪酸衍生物。 本发明还提供了诊断糖尿病的方法。 本发明另外提供了一种在不使用反应促进剂的情况下分析小于或等于五分钟的果糖胺浓度的方法。

公开(公告)号：US20240309074A1

公开(公告)日：2024-09-19

申请号：US18467204

申请日：2023-09-14

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Donald E. INGBER ,  Michael SUPER ,  Jeffrey Charles WAY ,  Mark J. CARTWRIGHT ,  Julia B. BERTHET ,  Dinah R. SUPER ,  Martin ROTTMAN ,  Alexander L WATTERS

IPC分类号： C07K16/12 ,  A61K47/68 ,  C07K14/42 ,  G01N33/543 ,  G01N33/569

CPC分类号： C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  C07K2319/33 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/30

摘要： Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：US20240077486A1

公开(公告)日：2024-03-07

申请号：US18270871

申请日：2022-01-05

申请人： FlowMetric Life Sciences, Inc.

发明人： Renold Julius Capocasale ,  Patrick Romano ,  Julie Ann Bick

IPC分类号： G01N33/574 ,  C07K14/42 ,  G01N33/543

CPC分类号： G01N33/57438 ,  C07K14/42 ,  G01N33/54353 ,  G01N2333/42 ,  G01N2400/02 ,  G01N2440/38

摘要： Described herein is a flow cytometry platform for the detection of glycosylated proteins in a clinical sample, along with the use of a flow cytometry platform for early disease diagnoses, for example, hepatocellular carcinoma (HCC). The use of the flow cytometry platform described herein allows for multiplexing and quantification of two or more biomarkers associated with the disease, for example, alpha-feto protein (AFP), alfa-feto protein-L3 (AFP-L3), or alpha-L-fucosidase (AFU) for HCC patients.

公开(公告)号：US11840578B2

公开(公告)日：2023-12-12

申请号：US17332243

申请日：2021-05-27

申请人： SYSMEX CORPORATION

发明人： Youichi Takahama ,  Hideki Matsuzaki ,  Takuya Kyoutou

IPC分类号： C07K16/42 ,  C07K16/06 ,  C07K19/00 ,  G01N33/68 ,  G01N33/96

CPC分类号： C07K16/4283 ,  C07K16/065 ,  C07K19/00 ,  G01N33/6854 ,  G01N33/6893 ,  G01N33/96 ,  G01N2333/42 ,  G01N2333/4724 ,  G01N2800/347

摘要： Disclosed is a calibrator comprising IgA having a biotin group and a biotin-binding site, the calibrator being used to obtain a concentration of an IgA aggregate in a sample.

公开(公告)号：US20180134756A1

公开(公告)日：2018-05-17

申请号：US15706144

申请日：2017-09-15

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Takashi Sato ,  Yasunori Chiba ,  Hiroaki Tateno ,  Hiroyuki Kaji ,  Masanori Goto ,  Hisashi Narimatsu

IPC分类号： C07K14/42 ,  G01N33/53

CPC分类号： C07K14/42 ,  G01N33/5308 ,  G01N2333/42 ,  G01N2400/00

摘要： A Wisteria floribunda monomeric lectin polypeptide is provided. The Wisteria floribunda monomeric lectin polypeptide includes any one of the amino acid sequences selected from the group consisting of: (1) the amino acid sequence represented by SEQ ID NO: 2; (2) the amino acid sequence defined in (1) above, except that one to 20 amino acids at positions other than Cys272 position is/are deleted, substituted, inserted, or added; and (3) the amino acid sequence defined in (1) or (2) above, further having an N-terminus deletion of one to 30 amino acids, in which Cys272 is alkylated, and the polypeptide is capable of specifically binding to a GalNAc terminal sugar chain.