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224 条记录 (耗时 0.033 秒)

    METHOD FOR DIAGNOSIS NON-SMALL-CELL-LUNG-CARCINOMA (NSCLC) AND CLASSIFICATION OF ITS SUBTYPES WITH DIFFERENT COMBINATIONS OF 6 PROTEIN BIOMARKERS
    63.
    发明申请
    METHOD FOR DIAGNOSIS NON-SMALL-CELL-LUNG-CARCINOMA (NSCLC) AND CLASSIFICATION OF ITS SUBTYPES WITH DIFFERENT COMBINATIONS OF 6 PROTEIN BIOMARKERS 审中-公开
    用于诊断非小细胞肺癌（NSCLC）的方法及其具有6种蛋白质生物标志物不同组合的亚型的分类

    公开(公告)号：US20120282631A1

    公开(公告)日：2012-11-08

    申请号：US13469316

    申请日：2012-05-11

    申请人： Samuel Chun Lap LO ,  Yoki Kwok Chu BUTT ,  Wallace Woon Fong LEUNG

    发明人： Samuel Chun Lap LO ,  Yoki Kwok Chu BUTT ,  Wallace Woon Fong LEUNG

    IPC分类号： G01N33/574 ,  C12Q1/26 ,  G01N27/26 ,  C12Q1/48 ,  G01N27/62 ,  G01N33/573

    CPC分类号： G01N33/57423 ,  G01N2333/4718 ,  G01N2333/908 ,  G01N2333/912 ,  G01N2333/95 ,  G01N2800/52

    摘要： A method of identifying and classifying non-small-cell lung carcinoma in a biological sample includes a first test wherein the expression level of at least one biomarker is measured in a sample, the at least one=biomarker is selected from a first group consisting of chaperonin, 2,3-bisphosphoglycerate mutase, thymidine phosphorylase, and minichromosome maintenance deficient protein 5, and comparing the measured expression levels against a normal expression level, wherein a significant difference diagnoses or aids in the diagnosis of non-small cell lung carcinoma; and a second test wherein the expression level of at least one biomarker is measured in the sample, the at least one biomarker is selected from a second group consisting of selenium binding protein and Napsin A, and comparing the measured expression level against a normal expression level, wherein a significant difference confirms or aids in confirmation of Non-Small Cell Carcinoma.

    摘要翻译： 在生物样品中鉴定和分类非小细胞肺癌的方法包括第一试验，其中在样品中测量至少一种生物标志物的表达水平，所述至少一种=生物标志物选自由 伴侣蛋白，2,3-二磷酸甘油酸变位酶，胸苷磷酸化酶和微染色体维持缺陷蛋白5，并比较测量的表达水平与正常表达水平，其中显着差异诊断或辅助诊断非小细胞肺癌; 以及第二测试，其中在样品中测量至少一种生物标记的表达水平，所述至少一种生物标志物选自由硒结合蛋白和Napsin A组成的第二组，并将测量的表达水平与正常表达水平进行比较 ，其中显着差异证实或有助于确认非小细胞癌。

    Assay methods using chemiluminescent detection of peroxidase
    66.
    发明授权
    Assay methods using chemiluminescent detection of peroxidase 有权
    使用化学发光检测过氧化物酶的测定方法

    公开(公告)号：US08106203B2

    公开(公告)日：2012-01-31

    申请号：US12476668

    申请日：2009-06-02

    申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua Xie

    发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Wenhua Xie

    IPC分类号： C07D219/00 ,  C12Q1/28

    CPC分类号： C07D219/02 ,  C07D219/04 ,  C07D219/06 ,  C07D409/04 ,  C07D409/06 ,  C07D411/04 ,  C07D411/06 ,  C07F9/64 ,  C07F9/65586 ,  C12Q1/28 ,  G01N33/581 ,  G01N2333/908

    摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.

    摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。

    Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase activity
    67.
    发明授权
    Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase activity 有权
    高纯度吩噻嗪N-烷基磺酸盐的制备及其在化学发光测定中用于测定过氧化物酶活性的用途

    公开(公告)号：US07855287B2

    公开(公告)日：2010-12-21

    申请号：US12010306

    申请日：2008-01-23

    申请人： Leopoldo Della Ciana

    发明人： Leopoldo Della Ciana

    IPC分类号： C07D279/26

    CPC分类号： C07D279/22 ,  G01N33/581 ,  G01N2333/908

    摘要： A process is described for preparing, efficiently and with a high degree of purity N-alkylsulfonates of phenothiazine. The process consists in (a) the preparation of the phenothiazine anion, and (b) the reaction of said anion with cyclic alkyl sulfonates, such as 1,3-propane sultone and 1,4-butane sultone. This process is simpler, more direct, and more efficient than the procedures currently used for the synthesis of N-alkylsulfonates derivatives of phenothiazine. In addition, the products obtained with this process are far more pure than those obtained through current procedures and therefore ideal for bioanalytical applications that require high sensitivity, such as assays based on the measurement of peroxidase activity using chemiluminescence.

    摘要翻译： 描述了制备，有效和高纯度吩噻嗪的N-烷基磺酸盐的方法。 该方法包括（a）吩噻嗪阴离子的制备，和（b）所述阴离子与环烷基磺酸盐如1,3-丙磺酸内酯和1,4-丁烷磺内酯的反应。 该方法比目前用于合成吩噻嗪的N-烷基磺酸盐衍生物的方法更简单，更直接且更有效。 此外，通过该方法获得的产品比通过当前方法获得的产品纯得多，因此对于需要高灵敏度的生物分析应用是理想的，例如基于使用化学发光测量过氧化物酶活性的测定法。

    Methods And Kits For In Situ Measurement of Enzyme Activity and Amount Using Single Measurement System
    68.
    发明申请
    Methods And Kits For In Situ Measurement of Enzyme Activity and Amount Using Single Measurement System 审中-公开
    使用单一测量系统原位测量酶活性和量的方法和试剂盒

    公开(公告)号：US20090286223A1

    公开(公告)日：2009-11-19

    申请号：US12302418

    申请日：2006-05-30

    申请人： Sue Goo Rhee ,  Jong Seo Lee ,  Sung Won Bae ,  Chun Seok Cho ,  Jeong Woo Shin ,  Young Ha Lee ,  Sook Yeon Lee ,  Yun Hwa Kim ,  So Mi Oh

    发明人： Sue Goo Rhee ,  Jong Seo Lee ,  Sung Won Bae ,  Chun Seok Cho ,  Jeong Woo Shin ,  Young Ha Lee ,  Sook Yeon Lee ,  Yun Hwa Kim ,  So Mi Oh

    IPC分类号： C12Q1/70 ,  G01N33/53

    CPC分类号： G01N33/573 ,  C12Q1/25 ,  G01N2333/908

    摘要： Disclosed are methods and kits for measuring in situ the activity and amount of an enzyme in a sample using a single measurement system. The method includes the steps of: (a) contacting a sample to a capturing agent having the capacity to bind to the enzyme to be analyzed and immobilized on a solid matrix; (b) measuring the activity of the enzyme captured by the capturing agent; (c) contacting a detection antibody specific to the enzyme captured by the capturing agent; and (d) detecting an antigen-antibody complex formed in the step (c). There is no need for separate measurement system to measure the activity and amount of an enzyme, as well as the above methods and kits permit to measure the precise activity and amount of an enzyme simultaneously because the measurement of enzyme activity and amount is carried out in a single and same measurement system as to the same sample in almost simultaneous manner.

    摘要翻译： 公开了用于使用单个测量系统原位测量样品中的酶的活性和量的方法和试剂盒。 该方法包括以下步骤：（a）将样品与能够结合待分析和固定在固体基质上的酶的捕获剂接触; （b）测量由捕获剂捕获的酶的活性; （c）使由捕获剂捕获的酶特异的检测抗体接触; 和（d）检测步骤（c）中形成的抗原 - 抗体复合物。 不需要单独的测量系统来测量酶的活性和量，以及上述方法和试剂盒允许同时测量酶的精确活性和量，因为酶活性和量的测量在 以同样的方式对相同样品进行单一和相同的测量系统。

    METHOD FOR INDUCING TUMOR APOPTOSIS BY INCREASING NITRIC OXIDE LEVELS
    69.
    发明申请
    METHOD FOR INDUCING TUMOR APOPTOSIS BY INCREASING NITRIC OXIDE LEVELS 有权
    通过增加氧化氮水平诱导肿瘤病变的方法

    公开(公告)号：US20090264398A1

    公开(公告)日：2009-10-22

    申请号：US12438791

    申请日：2007-08-07

    申请人： Georg Bauer

    发明人： Georg Bauer

    IPC分类号： A61K31/397 ,  C12Q1/18 ,  A61K31/337 ,  A61P35/00

    CPC分类号： G01N33/5011 ,  A61K31/19 ,  A61K31/195 ,  A61K31/415 ,  A61K31/4174 ,  A61K31/4196 ,  A61K31/496 ,  C12Q1/30 ,  G01N2333/908 ,  G01N2333/978 ,  G01N2510/00 ,  A61K2300/00

    摘要： The present invention refers to a method for inducing tumor apoptosis by influencing the ROS (reactive oxygen species) signaling pathway in tumor cells. Increasing the level of ROS leads to the selective inactivation of a tumor cell catalase and subsequently to an apoptosis of these cells. The level of ROS can be increased by increasing the level of nitric oxide through inhibition of the enzymes nitric oxide dioxygenase or arginase.According to the present invention inhibitors of the nitric oxide dioxygenase or arginase can be used for the manufacture of a medicament for the treatment of cancer. The present invention further provides a method for identifying compounds which can be used for the treatment of cancer, wherein the method allows to specifically identify compounds which induce apoptosis through the ROS signaling pathway. The present invention also provides a kit for identifying compounds which induce tumor apoptosis by inactivating a catalase on the tumor cell surface.

    摘要翻译： 本发明涉及通过影响肿瘤细胞中的ROS（活性氧）信号通路诱导肿瘤细胞凋亡的方法。 增加ROS的水平导致肿瘤细胞过氧化氢酶的选择性失活，然后导致这些细胞的细胞凋亡。 可以通过抑制一氧化氮双加氧酶或精氨酸酶来增加一氧化氮的水平来增加ROS的水平。 根据本发明，一氧化氮双加氧酶或精氨酸酶的抑制剂可用于制备用于治疗癌症的药物。 本发明还提供了一种用于鉴定可用于治疗癌症的化合物的方法，其中所述方法允许特异性鉴定通过ROS信号通路诱导细胞凋亡的化合物。 本发明还提供了一种用于鉴定通过灭活肿瘤细胞表面上的过氧化氢酶诱导肿瘤细胞凋亡的化合物的试剂盒。