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124 条记录 (耗时 0.055 秒)

    Promoter engineering and genetic control
    61.
    发明授权
    Promoter engineering and genetic control 有权
    启动子工程和遗传控制

    公开(公告)号：US08110672B2

    公开(公告)日：2012-02-07

    申请号：US11411212

    申请日：2006-04-26

    申请人： Gregory Stephanopoulos ,  Hal S. Alper ,  Curt Fischer ,  Elke Nevoigt

    发明人： Gregory Stephanopoulos ,  Hal S. Alper ,  Curt Fischer ,  Elke Nevoigt

    IPC分类号： C07H21/04 ,  C40B40/02 ,  C40B40/06 ,  C40B20/08

    CPC分类号： C12N15/1086

    摘要： The present invention relates to expression cassettes libraries of expression vectors comprising the same, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of another in the library and cells comprising the same. Methods utilizing either the libraries or cells of this invention, in optimizing gene expression, protein expression, or optimized gene or protein delivery are described.

    摘要翻译： 本发明涉及包含该表达载体的表达载体的表达盒文库，其中每个载体包含至少一个目的基因和可操作地连接到其上的启动子，其中每个启动子包含核酸，其序列相对于另一个的序列是随机突变的 在图书馆和包含它的细胞中。 描述了利用本发明的文库或细胞，优化基因表达，蛋白质表达或优化的基因或蛋白质递送的方法。

    METHOD FOR IMMOBILIZING NUCLEIC ACIDS ON A SUPPORT
    63.
    发明申请
    METHOD FOR IMMOBILIZING NUCLEIC ACIDS ON A SUPPORT 有权
    用于在支持物上固定核酸的方法

    公开(公告)号：US20110319275A1

    公开(公告)日：2011-12-29

    申请号：US13121206

    申请日：2009-09-22

    申请人： Anke Pierik ,  Hendrik Roelof Stapert

    发明人： Anke Pierik ,  Hendrik Roelof Stapert

    IPC分类号： C40B20/08 ,  C40B50/18 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  C12Q2525/173

    摘要： The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention.

    摘要翻译： 本发明涉及用于将核酸固定在载体上的方法，其包括提供具有仅一种碱基类型的核苷酸序列的核酸和通过光交联将所述核酸固定在载体上，其中所述交联通过 光在约300-500nm的波长处进行，优选在365nm的波长下进行。 本发明还涉及用于分析根据本发明固定的核酸的方法，其包括将固定的核酸与互补和错配片段杂交。 此外，本发明涉及通过本发明的方法可获得的固定化核酸，使用相应地固定的核酸来生产核酸阵列和诊断试剂盒，其包含根据本发明固定的核酸阵列 发明。

    Plant nucelotide sequences and corresponding polypeptides
    65.
    发明申请
    Plant nucelotide sequences and corresponding polypeptides 有权
    植物胡萝卜素序列和相应的多肽

    公开(公告)号：US20110177228A1

    公开(公告)日：2011-07-21

    申请号：US12583039

    申请日：2009-08-12

    申请人： Nickolai Alexandrov ,  Vyacheslav Brover ,  Maxim Troukhan

    发明人： Nickolai Alexandrov ,  Vyacheslav Brover ,  Maxim Troukhan

    IPC分类号： A23L1/212 ,  C07H21/04 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10 ,  C12Q1/68 ,  A01H1/00 ,  C40B20/08 ,  A23K1/00

    CPC分类号： C07K14/415 ,  A23K10/30

    摘要： The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides. The present invention further relates to the uses of these nucleic acid molecules and polypeptides. For example, the nucleic acid molecules and polypeptides could be used in making enzymes or used to make plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.

    摘要翻译： 本发明涉及分离的核酸分子及其相应的编码多肽。 本发明还涉及这些核酸分子和多肽的用途。 例如，核酸分子和多肽可用于制备酶或用于制备植物的植物，植物细胞，植物材料或种子，所述植物具有这样的调节的生长或表型特征，相对于在相似的条件下生长的野生型植物 条件。

    SYSTEM AND METHOD FOR NUCLEIC ACIDS SEQUENCING BY PHASED SYNTHESIS
    67.
    发明申请
    SYSTEM AND METHOD FOR NUCLEIC ACIDS SEQUENCING BY PHASED SYNTHESIS 有权
    通过PHASED合成序列测定核酸的系统和方法

    公开(公告)号：US20090312188A1

    公开(公告)日：2009-12-17

    申请号：US12481799

    申请日：2009-06-10

    申请人： Reuven Duer ,  James Herron

    发明人： Reuven Duer ,  James Herron

    IPC分类号： C40B20/08 ,  C40B60/10

    CPC分类号： G02B27/56 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/00704 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0819 ,  B82Y30/00 ,  C12N15/1068 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6452 ,  G01N21/648 ,  G01N2021/6441 ,  G02B6/24 ,  C12Q2523/319 ,  C12Q2535/107 ,  C12Q2565/607

    摘要： A system and methods of sequencing a nucleic acid by detecting the identity of a fluorescent nucleotide analogue incorporated at the 3′ end of a growing nucleic acid strand are provided. One method includes the steps of (a) immobilizing a plurality of complexes comprising a template nucleic acid, a primer configured to hybridize to the template and a polymerase, at a plurality of optical sensing sites of a substrate, wherein the substrate is part of a waveguide-based optical scanning system; (b) extending the primer by a single nucleotide with the polymerase and one or more fluorescent nucleotide analogues using a polymerase extension reaction, wherein each type of fluorescent nucleotide analogue comprises a unique fluorescent tag optionally configured to inhibit further primer extension and/or a blocking agent at the 3′ end and wherein incorporation of the fluorescent nucleotide analogue reversibly terminates the polymerase extension reaction; (c) detecting the unique tag of the fluorescent nucleotide analogue by optically scanning the substrate using the optical scanning system to identify the fluorescent nucleotide analogue incorporated by the polymerase reaction; (d) recording the results of the optical scanning of the substrate; (e) reversing the termination of the polymerase extension reaction by providing a photo-cleaving pulse of light to one or more of the optical sensing sites of the substrate to cleave the fluorescent tag or the blocking agent; and (f) repeating steps (b) through (e).

    摘要翻译： 提供了通过检测在生长的核酸链的3'末端引入的荧光核苷酸类似物的身份来测序核酸的系统和方法。 一种方法包括以下步骤：（a）在衬底的多个光学感测位置处固定包含模板核酸的多个复合物，配置为与模板杂交的引物和聚合酶，其中所述底物是 基于波导的光学扫描系统; （b）使用聚合酶延伸反应，使用聚合酶和一个或多个荧光核苷酸类似物将引物延伸单个核苷酸，其中每种类型的荧光核苷酸类似物包含独特的荧光标签，其可选地配置为抑制进一步的引物延伸和/或阻断 3'端的试剂，其中掺入荧光核苷酸类似物可逆地终止聚合酶延伸反应; （c）通过使用光学扫描系统光学扫描底物来检测荧光核苷酸类似物的唯一标记，以鉴定通过聚合酶反应引入的荧光核苷酸类似物; （d）记录基板的光学扫描的结果; （e）通过向衬底的一个或多个光学感测位置提供光切割脉冲以切割荧光标签或封闭剂来反转聚合酶延伸反应的终止; 和（f）重复步骤（b）至（e）。