公开(公告)号：US5140038A

公开(公告)日：1992-08-18

申请号：US401254

申请日：1989-08-31

申请人： Ernst-Christian Witte ,  Karlheinz Stegmeier ,  Liesel Doerge

发明人： Ernst-Christian Witte ,  Karlheinz Stegmeier ,  Liesel Doerge

IPC分类号： A61K31/18 ,  A61K31/195 ,  A61K31/22 ,  A61K31/41 ,  A61P1/04 ,  A61P3/00 ,  A61P7/02 ,  A61P9/00 ,  A61P11/00 ,  A61P13/02 ,  A61P15/00 ,  A61P25/28 ,  C07C311/19 ,  C07C323/65 ,  C07D257/04 ,  C07D257/06

CPC分类号： C07D257/04 ,  C07C311/19 ,  C07D257/06 ,  C07C2101/14

摘要： The present invention provides sulphonamides of the general formula: ##STR1## wherein R.sup.1 and R.sup.2, which can be the same or different, are hydrogen or halogen atoms or C.sub.1 -C.sub.6 -alkyl, trifluoro-methyl, cyano, carboxyl, alkoxycarbonyl, aminocarbonyl, N-alkylaminocarbonyl or N,N-dialkylaminocarbonyl radicals or, when R.sup.1 and R.sup.2 are alkyl radicals ortho to one another, R.sup.1 and R.sup.2, together with the carbon atoms to which they are attached, form a saturated or unsaturated C.sub.5 -C.sub.7 -alkylene ring, R.sup.3 is a hydrogen atom, an alkyl radical containing up to 6 carbon atoms, an acyl radical, a phenylalkyl or phenylalkenyl radical, the phenyl moiety of which can be substituted by halogen, alkyl or trifluoromethyl, C is a --(CH.sub.2).sub.m -- radical, in which m is 0, 1, 2 or 3, or a branched C.sub.2 -C.sub.5 alkylene radical, whereby a methylene radical --CH.sub.2 -- of the group C can be replaced by an oxygen or sulphur atom or by a hydroxymethylene radical --CHOH-- or carbonyl group --CO--, B is a 1,2-, 1,3-, 1,4-cyclohexylidene or 1,2- or 1,3-cyclopentylidene radical.

摘要翻译： 本发明提供通式如下的磺酰胺：其中R 1和R 2可以相同或不同，为氢或卤素原子或C 1 -C 6烷基，三氟甲基，氰基，羧基，烷氧基羰基 ，氨基羰基，N-烷基氨基羰基或N，N-二烷基氨基羰基，或者当R 1和R 2是彼此相邻的烷基时，R 1和R 2与它们连接的碳原子一起形成饱和或不饱和的C5-C7 - 亚烷基环，R3是氢原子，含有至多6个碳原子的烷基，酰基，苯基烷基或苯基烯基，其苯基部分可以被卤素，烷基或三氟甲基取代，C是 - （ CH 2）m - 基，其中m为0,1,2或3，或支链C 2 -C 5亚烷基，其中基团C的亚甲基-CH 2可以被氧或硫原子或羟基亚甲基 基团-CHOH-或羰基-CO-，B是1,2-，1,3-，1,4-亚环己基或1,2-或 1,3-亚环戊基。

公开(公告)号：US5132208A

公开(公告)日：1992-07-21

申请号：US189782

申请日：1988-05-03

申请人： Helmut Freitag ,  Hans-Erich Wilk ,  Anselm Rothe ,  Heino Eikmeier

发明人： Helmut Freitag ,  Hans-Erich Wilk ,  Anselm Rothe ,  Heino Eikmeier

IPC分类号： G01N31/22 ,  C12Q1/00 ,  G01N33/52 ,  G01N33/543

CPC分类号： G01N33/525

摘要： The present invention provides a test carrier for the analysis of a sample liquid and especially of a body fluid, having a porous test layer (8, 13) which contains a solid component (9, 17), wherein the solid component (9, 17) is coated with a protein which is insoluble in the sample liquid under the test conditions. A process for the production of this test layer is also disclosed, wherein the protein is dissolved in a solvent under conditions under which the solubility of the protein is sufficiently high in order to dissolve a certain minimum amount of the protein, the solid component of the test layer is contracted with the solution and the solubility is reduced to such an extent that the component is coated by the precipitating protein.

摘要翻译： 本发明提供了一种用于分析样品液体，特别是体液的测试载体，其具有含有固体组分（9,17）的多孔测试层（8,13），其中固体组分（9,17） ）在测试条件下涂覆有不溶于样品液体的蛋白质。 还公开了用于生产该测试层的方法，其中将蛋白质溶解在溶解有蛋白质的溶解度足够高以便溶解某些最低量的蛋白质的条件下，将固体成分 试验层与溶液一起收缩，溶解度降低至组分被沉淀蛋白包被的程度。

公开(公告)号：US5114847A

公开(公告)日：1992-05-19

申请号：US590602

申请日：1990-09-25

申请人： Herbert Jungfer ,  Heinrich Barchet ,  Winfried Albert

发明人： Herbert Jungfer ,  Heinrich Barchet ,  Winfried Albert

IPC分类号： G01N33/577 ,  A61K35/12 ,  A61K38/16 ,  A61K39/395 ,  C07K14/00 ,  C07K14/52 ,  C07K16/00 ,  C12N15/00 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/48

CPC分类号： C07K16/00 ,  C07K14/00 ,  C07K14/52 ,  C12N15/00

摘要： A method for obtaining permanently culturable human and animal cell lines, as well as uses for these cell lines, are disclosed. The method involves fusing cells of a non-immortal cell line with cytoplasts or cytoplasma fractions of "immortal", transformed cells such as immortal myeloma cells, ascites-tumor cells or Epstein Barr Virus infected cells. Once fusion takes place the product is a permanently culturable variant of the previously normal cell line.

摘要翻译： 公开了获得永久培养的人和动物细胞系的方法以及这些细胞系的用途。 该方法包括将非永生细胞系的细胞与“永生”，转化细胞如永生性骨髓瘤细胞，腹水肿瘤细胞或爱泼斯坦巴尔病毒感染细胞的细胞质或细胞浆级分融合。 一旦发生融合，该产品是以前正常细胞系的永久可培养的变体。

公开(公告)号：US5108913A

公开(公告)日：1992-04-28

申请号：US545092

申请日：1990-06-27

申请人： Elli Rauscher ,  Ulrich Neumann ,  August W. Wahlefeld ,  Alexander Hagen ,  Wolfgang Gruber ,  Joachim Ziegenhorn ,  Eugen Schaich ,  Ulfert Deneke ,  Michal Gerhard ,  Gunter Weimann

发明人： Elli Rauscher ,  Ulrich Neumann ,  August W. Wahlefeld ,  Alexander Hagen ,  Wolfgang Gruber ,  Joachim Ziegenhorn ,  Eugen Schaich ,  Ulfert Deneke ,  Michal Gerhard ,  Gunter Weimann

IPC分类号： G01N33/50 ,  C07H15/04 ,  C07H15/203 ,  C12Q1/40

CPC分类号： C12Q1/40 ,  C12Q2334/10 ,  Y10S435/805 ,  Y10S435/81

摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.

公开(公告)号：US5104811A

公开(公告)日：1992-04-14

申请号：US134950

申请日：1987-12-18

申请人： Dieter Berger ,  Wolfgang-Reinhold Knappe ,  Robert Lorenz ,  Henry M. Grage, Jr. ,  Mark T. Skarstedt ,  Bernward Sojka ,  Manfred Bleisteiner

发明人： Dieter Berger ,  Wolfgang-Reinhold Knappe ,  Robert Lorenz ,  Henry M. Grage, Jr. ,  Mark T. Skarstedt ,  Bernward Sojka ,  Manfred Bleisteiner

IPC分类号： G01N31/22 ,  C12M1/34 ,  G01N21/77 ,  G01N33/49 ,  G01N33/52 ,  G01N33/543 ,  G01N33/558

CPC分类号： G01N33/525 ,  G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/81

摘要： On a carrier layer there are arranged several test layers which are at least partly in fluid contact with one another, enabling liquid exchange. On the carrier layer are arranged an application zone, a detection zone for the production of a detectable signal characteristic of the analytical determination, which contains at least one said test layer which is a reaction layer, and an absorption zone with a test layer which is an absorptive layer of an absorbent material, wherein the reaction layer and absorption zone are positioned next to each other. Between the application and the absorption zone is a capillary-active transport path on the carrier layer which connects the application zone and the absorption zone. The reaction layer is arranged parallel to the transport path between the application zone and the absorption zone in such a manner that it is in liquid contact with a liquid transported in the transport path. The absorption zone therefore absorbs excess liquid and permits dosing the reaction layer with a reproducible amount of sample.

摘要翻译： 在载体层上布置有几个测试层，其至少部分地彼此流体接触，从而实现液体交换。 在载体层上布置有应用区域，用于产生分析测定特征的可检测信号的检测区域，其包含至少一个作为反应层的测试层，以及具有测试层的吸收区，其为 吸收材料的吸收层，其中反应层和吸收区彼此相邻放置。 在应用和吸收区之间是在载体层上的连接施加区和吸收区的毛细管 - 主动传输路径。 反应层以与输送路径中输送的液体液体接触的方式平行于施加区域和吸收区域之间的输送路径布置。 因此，吸收区吸收多余的液体，并允许用可重复量的样品给反应层。

公开(公告)号：US5084395A

公开(公告)日：1992-01-28

申请号：US554733

申请日：1990-07-18

申请人： Lee Bouse ,  Michael Phillips

发明人： Lee Bouse ,  Michael Phillips

IPC分类号： C12Q1/28 ,  G01N21/78 ,  G01N31/22 ,  G01N33/52

CPC分类号： C12Q1/28 ,  G01N21/78 ,  G01N31/22 ,  G01N31/221 ,  G01N33/52 ,  G01N33/525 ,  C12Q2326/12

摘要： The invention relates to a composition useful in determining an analyte in a sample. The composition contains an indicator system which forms a detectable signal in the presence of said analyte, and which is not a coupling system, as well as a pyrogallol derivative. The pyrogallol derivative causes uniform formation of detectable signal. Also described are kits useful in determining an analyte, apparatus which incorporate the composition, and a method for determining an analyte using the composition.

摘要翻译： 本发明涉及可用于测定样品中分析物的组合物。 组合物含有指示剂系统，其在所述分析物存在下形成可检测信号，并且其不是偶联系统，以及连苯三酚衍生物。 连苯三酚衍生物导致可检测信号的均匀形成。 还描述了可用于确定分析物的试剂盒，掺入组合物的装置，以及使用该组合物测定分析物的方法。

公开(公告)号：US5071868A

公开(公告)日：1991-12-10

申请号：US631641

申请日：1991-01-28

申请人： Herbert Leinert

发明人： Herbert Leinert

IPC分类号： A61K31/40 ,  A61K31/403 ,  A61K31/44 ,  A61K31/4427 ,  A61K31/443 ,  A61P9/08 ,  A61P9/12 ,  A61P25/02 ,  C07C29/09 ,  C07D209/56 ,  C07D209/88 ,  C07D303/08 ,  C07D303/12 ,  C07D303/14 ,  C07D303/16 ,  C07D317/20 ,  C07D401/12 ,  C07D405/12 ,  C07D491/056

CPC分类号： C07D405/12 ,  C07C309/00 ,  C07D209/88 ,  C07D303/14 ,  C07D303/16 ,  C07D317/20 ,  Y10S514/913 ,  Y10S514/929

摘要： A process for the preparation of S- or R-carbazole derivatives of the general formula: ##STR1## in which R is an unsubstituted or substituted amino radical and pharmacologically acceptable salts, by either reacting R-(-)-epichlorohydrin (for the S-carbozole derivative); or reacting an S-epoxide derivative of the general formula: ##STR2## in which R.sub.1 is the residue of a substituted sulphonic acid derivative (for the R-carbazole derivative); with 4-hydroxycarbazole and then with ammonia or a substituted amine of the general formula RH, and recovering the compound or converting it to a pharmacologically acceptable salt.The new R-(+)- and S(-)-carbazole derivatives provided by the inventive process have unexpected beta blocking and vasodilatory properties and are useful in pharmaceutical compositions. R-(+)-carbazole derivatives are also useful for the treatment of glaucoma.

公开(公告)号：US5055195A

公开(公告)日：1991-10-08

申请号：US77003

申请日：1987-07-20

申请人： Heinz-Friedrich Trasch ,  Erwin Endres ,  Andreas Trost ,  Walter Rittersdorf

发明人： Heinz-Friedrich Trasch ,  Erwin Endres ,  Andreas Trost ,  Walter Rittersdorf

IPC分类号： A61K35/16 ,  A61K35/18 ,  G01N33/48 ,  G01N33/49 ,  G01N33/52

CPC分类号： A61K35/16 ,  A61K35/18 ,  G01N33/48 ,  G01N33/525 ,  Y10T436/25375

摘要： The present invention provides erythrocyte-retention substrates of the general formula:R.sub.1 --Sp--R.sub.2in which Sp is a bridge serving as a spacer, R.sub.1 is a strongly polar group and R.sub.2 is also a strongly polar group or, when Sp is an electron-conductive group, R.sub.2 can also be a polarizable group.The present invention also provides a process for the separation of erythrocytes from whole blood, wherein whole blood is brought into contact with one of the above substrates and subsequently filtered through a filter layer and the separated plasma obtained.

摘要翻译： 本发明提供了以下通式的红细胞保留底物：其中Sp为桥作为间隔基的R1-Sp-R2，R1为强极性基团，R2为强极性基团，或当Sp为电子 导电基团，R2也可以是可极化的基团。 本发明还提供了从全血中分离红细胞的方法，其中使全血与上述底物接触，然后通过过滤层过滤并分离得到的血浆。

公开(公告)号：US5037849A

公开(公告)日：1991-08-06

申请号：US424452

申请日：1989-10-20

申请人： Herbert Simon ,  Helmut Michel ,  Michael Schultz ,  Wolfgang Bartsch

发明人： Herbert Simon ,  Helmut Michel ,  Michael Schultz ,  Wolfgang Bartsch

IPC分类号： A61K31/21 ,  A61K31/395 ,  A61K31/40 ,  A61K31/445 ,  A61P9/00 ,  A61P9/08 ,  A61P9/10 ,  C07C235/10 ,  C07C235/26 ,  C07C235/40 ,  C07C237/08 ,  C07C237/18 ,  C07C275/10 ,  C07C275/14 ,  C07C275/16 ,  C07C275/26 ,  C07D211/06 ,  C07D295/13 ,  C07D295/14 ,  C07D295/215

CPC分类号： C07D295/215 ,  A61K31/21 ,  C07C235/10 ,  C07C235/26 ,  C07C235/40 ,  C07C237/08 ,  C07C275/10 ,  C07C275/14 ,  C07C275/16 ,  C07C275/26 ,  C07D295/13 ,  C07C2101/14

摘要： The present invention provides pharmaceutical compositions effective against angina-like heart and circulatory diseases containing at least one nitroxyalkylamine derivative of the general formula: ##STR1## wherein R.sub.1 is a hydrogen atom, a lower-alkyl radical, a C.sub.3 -C.sub.8 -cycloalkyl or C.sub.3 -C.sub.8 -cycloalkenyl radical, an aminocarbonyl, C.sub.1 -C.sub.6 -mono- or di-alkylaminocarbonyl radical, R.sup.2 is a hydrogen atom, a lower-alkyl radical or a C.sub.3 -C.sub.8 -cycloalkyl or cycloalkenyl radical or R.sup.2, together with R.sup.1 and the nitrogen atom to which they are attached, form a heteroaliphatic ring containing up to 6 carbon atoms, A is a valency bond or a straight-chained or branched lower-alkylene radical, in which a --CH.sub.2 -- group can be replaced by a cycloalkylene radical, B is a lower-alkylene radical, in which a --CH.sub.2 -- group can be replaced by a cycloalkylene radical and X is an --NR.sup.3 --CO-- or --CO--NR.sup.3 -- radical, in which R.sup.3 is a hydrogen atom or a lower-alkyl radical, when X is an --NR.sup.3 --CO-- radical, R.sup.3, together with the nitrogen atom and a carbon atom of the group A, bridge a heterocyclic ring containing 4 to 6 carbon atoms or R.sup.3, together with R.sup.2, A and the two nitrogen atoms, bridge a heterocyclic ring containing 3 to 5 carbon atoms; and the optically-active forms and physiologically acceptable salts thereof.

摘要翻译： 本发明提供了有效抗心绞痛的心脏和循环系统疾病的药物组合物，其含有至少一种以下通式的硝基烷基胺衍生物：其中R 1是氢原子，低级烷基，C 3 -C 8 - 环烷基或C 3 -C 8 - 环烯基，氨基羰基，C 1 -C 6 - 单 - 或二 - 烷基氨基羰基，R 2是氢原子，低级烷基或C 3 -C 8 - 环烷基或环烯基或R 2，连同 R1和它们所连接的氮原子形成含有至多6个碳原子的杂脂族环，A是价键或直链或支链的低级亚烷基，其中-CH 2 - 基团可以被 亚环烷基，B是低级亚烷基，其中-CH 2 - 基团可被亚环烷基代替，X是-NR 3 -CO-或-CO-NR 3 - 基，其中R 3是氢原子 或低级烷基，当X是-NR 3 -CO-基时，R 3与硝基一起形成 原子和A基团的碳原子，桥接含有4至6个碳原子的杂环或R3与R2，A和两个氮原子一起桥接含有3至5个碳原子的杂环; 和其光学活性形式和生理上可接受的盐。

公开(公告)号：US5030641A

公开(公告)日：1991-07-09

申请号：US424451

申请日：1989-10-20

申请人： Herbert Simon ,  Helmut Michel ,  Michael Schultz ,  Wolfgang Bartsch

发明人： Herbert Simon ,  Helmut Michel ,  Michael Schultz ,  Wolfgang Bartsch

IPC分类号： C07D211/56 ,  A61K31/40 ,  A61K31/445 ,  A61K31/4465 ,  A61K31/4468 ,  A61P9/12 ,  C07D207/16 ,  C07D211/46 ,  C07D211/58 ,  C07D211/60 ,  C07D211/62

CPC分类号： C07D211/58 ,  C07D207/16 ,  C07D211/46 ,  C07D211/60 ,  C07D211/62

摘要： The present invention provides anti-angina pharmaceutical compositions containing at least one compound of the formula: ##STR1## wherein n is 1 or 2, R is a hydrogen atom or an H-(C.sub.1 -C.sub.8)-alkylene, hydroxy-(C.sub.1 -C.sub.8)-alkylene, R.sup.1 R.sup.2 N-(C.sub.1 -C.sub.8)-alkylene, R.sup.1 R.sup.2 N-(C.sub.1 -C.sub.8)-alkylene-CO--, R.sup.1 R.sup.2 N-CO-NR.sup.3 -(C.sub.1 -C.sub.8)-alkylene or R.sup.1 R.sup.2 N-CO-- radical, A is a valency bond or an --NR.sup.4 --CO-- or --CO--NR.sup.4 -- radical, R.sup.4 is a hydrogen atom or a straight-chained or branched, saturated or unsaturated of cyclic alkyl radical containing up to 6 carbon atoms and B is a valency bond or a straight-chained or branched alkylene chain containing up to 8 carbon atoms, in which a --CH.sub.2 -- group can be replaced by a cycloalkylene radical containing 3 to 7 carbon atoms.

摘要翻译： 本发明提供含有至少一种下式的化合物的抗心绞痛药物组合物：其中n为1或2，R为氢原子或H-（C1-C8） - 亚烷基，羟基 - （C1-C8） - 亚烷基，R1R2N-（C1-C8） - 亚烷基，R1R2N-（C1-C8） - 亚烷基-CO-，R1R2N-CO-NR3-（C1-C8） - 亚烷基或R1R2N-CO- A是价键或-NR4-CO-或-CO-NR4-基，R4是氢原子或直链或支链，饱和或不饱和的含有至多6个碳原子的环烷基，B是 含有至多8个碳原子的价键或直链或支链亚烷基链，其中-CH 2 - 基团可被含有3至7个碳原子的亚环烷基代替。