公开(公告)号：US20160222363A1

公开(公告)日：2016-08-04

申请号：US15012317

申请日：2016-02-01

Applicant: Genia Technologies, Inc. ,  Roche Molecular Systems, Inc.

Inventor： Aruna AYER ,  Arkadiusz BIBILLO ,  Cleoma Renetta ARNOLD ,  Charles Wayan SCHWAB ,  Eileen THAI ,  Ilya LEDERMAN ,  Colin Alexander MCGAW ,  Tyler O'Brien SHULTZ ,  Barbara ECKERT ,  Shawn SUKO ,  Mara BOENITZ-DULAT ,  Bigna WOERSDOERFER ,  David Daniel WUNDERLICH

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.

Abstract translation: 本文描述的是具有至少一个选自H223，N224，Y225，H227，I295，Y342，T343，I357，S360，L361，I363，S365Q，S366，Y367，P368，D417，E475，Y476， F478，K518，H527，T529，M531，N535，G539，P542，N545，Q546，A547，L549，I550，N552，G553，F558，A596，G603，A610，V615，Y622，C623，D624，I628，Y629， R632，N635，M641，A643，I644，T647，I648，T651，I652，K655，W656，D657，V658，H660，F662，L690及其组合。

公开(公告)号：US20160178554A1

公开(公告)日：2016-06-23

申请号：US14971667

申请日：2015-12-16

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/22 ,  C12Q1/68 ,  G01N33/487 ,  G01N27/447

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/3273 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

Abstract translation: 公开了分析分子的方法。 使用由复位信号控制的开关，电压源选择性地连接到电容器或从电容器断开。 当电压源连接到电容器时，电容被存储在电容器中。 当电压源与电容器断开时，电容器通过膜中的纳米孔排出。 确定复位信号的占空比，使得电压源和电容器连接至复位信号周期的至少十分之一，并且对于复位信号周期的剩余部分断开，使得跨越纳米孔的电压 在维持连接的复位信号周期的部分期间保持在比不保持连接的复位信号周期的剩余部分期间更高的电平。

公开(公告)号：US20160146203A1

公开(公告)日：2016-05-26

申请号：US14867922

申请日：2015-09-28

Applicant: Genia Technologies, Inc.

Inventor： Robert A. Yuan

IPC: F04B53/10

CPC classification number: F04B53/10 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0663 ,  B01L2300/0627 ,  B01L2300/0867 ,  B01L2400/0644 ,  F16K27/003 ,  G01N33/48721

Abstract: A delivery system for a sensor chip includes a plurality of selectable ports and a two-way pump port selectively connectable to each of the selectable ports. The two-way pump port is configured to allow material to be drawn or delivered from or to the two-way pump port. The delivery system also includes a chamber and a bypass waste channel that is selectively connectable to the two-way pump port. The plurality of selectable ports includes a selectable chamber port connected to the chamber and the chamber has a chamber waste exit. Material may selectively flow through the chamber to a waste collection via the chamber waste exit or flow to the waste collection via the bypass waste channel that bypasses the chamber waste exit.

Abstract translation: 用于传感器芯片的传送系统包括多个可选端口和可选择性地连接到每个可选端口的双向泵端口。 双向泵端口被配置为允许材料从双向泵端口被吸入或输送到双向泵端口。 输送系统还包括可选择性地连接到双向泵端口的室和旁路废气通道。 多个可选择的端口包括连接到室的可选择的室端口，并且该室具有室废物出口。 材料可以选择性地通过室废物出口流过废物收集物，或通过绕过室废物出口的旁路废物通道流入废物收集物。

公开(公告)号：US20150107996A1

公开(公告)日：2015-04-23

申请号：US14056795

申请日：2013-10-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

Abstract translation: 公开了鉴定分子的方法。 通过在第一周期期间将一个第一电压信号施加到一对电极上，将一个分子吸引到纳米孔，其中第一电压信号引起通过纳米孔的第一离子电流，其指示分子的一部分的性质接近于 纳米孔。 在第二时段期间，通过在所述一对电极施加第二电压信号，从所述纳米孔释放所述分子，其中所述第二电压信号引起通过所述纳米孔的第二离子电流。 至少部分地基于通过包含第一离子电流和第二离子电流的纳米孔的净离子电流确定第一周期和第二周期。

公开(公告)号：US20150094214A1

公开(公告)日：2015-04-02

申请号：US14513642

申请日：2014-10-14

Applicant: Genia Technologies, Inc

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: C12Q1/68 ,  G01N27/327 ,  G01N27/447

CPC classification number: C12Q1/6816 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/3278 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  G01N2015/0038 ,  Y10T29/49002 ,  Y10T29/49117 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.

Abstract translation: 用于表征分子的技术在本文中描述。 在一个实例中，分子的一部分被捕获在纳米孔中，跨越纳米孔施加可变电压，直到分子的被捕获部分在纳米孔内移动，并且基于影响运动所需的电刺激来表征分子 纳米孔内的分子的截留部分的至少一部分。

公开(公告)号：US20140134616A1

公开(公告)日：2014-05-15

申请号：US14073445

申请日：2013-11-06

Applicant: Genia Technologies, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum ,  Michael Dorwart

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

Abstract translation: 本公开提供了用于对核酸样品进行测序的芯片，系统和方法。 将标记的核苷酸提供到包含膜中的纳米孔的反应室中。 标记的核苷酸的单个标记的核苷酸可以含有与核苷酸偶联的标签，该标签借助于纳米孔可检测。 接下来，标记的核苷酸的单个标记的核苷酸可以掺入与衍生自核酸样品的单链核酸分子互补的生长链中。 借助纳米孔，可以在掺入单个标记的核苷酸时检测与单个标记的核苷酸相关的标签。 当标签从核苷酸释放时，可以借助纳米孔检测标签。

公开(公告)号：US20140014513A1

公开(公告)日：2014-01-16

申请号：US13893142

申请日：2013-05-13

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: G01N27/447

CPC classification number: C12Q1/6816 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/3278 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  G01N2015/0038 ,  Y10T29/49002 ,  Y10T29/49117 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.

Abstract translation: 用于表征分子的技术在本文中描述。 在一个实例中，分子的一部分被捕获在纳米孔中，跨越纳米孔施加可变电压，直到分子的被捕获部分在纳米孔内移动，并且基于影响运动所需的电刺激来表征分子 纳米孔内的分子的截留部分的至少一部分。

公开(公告)号：US20130237460A1

公开(公告)日：2013-09-12

申请号：US13777879

申请日：2013-02-26

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Kevin Deierling ,  Roger J.A. Chen ,  David J. Fullagar

IPC: C12Q1/68

CPC classification number: G01N27/4473 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721 ,  Y10T436/11 ,  C12Q2565/631 ,  C12Q2565/607

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

公开(公告)号：US20200216887A1

公开(公告)日：2020-07-09

申请号：US16071406

申请日：2017-01-20

Applicant: Genia Technologies, Inc. ,  ROCHE DIAGNOSTICS GMBH

Inventor： Timothy Kellogg Craig ,  Christos Tzitzilonis ,  Alexander H. Yang ,  Liv E. Jensen ,  Marshall Porter ,  Charlotte Yang ,  Corissa Harris ,  Matt Dipetro

IPC: C12Q1/6869 ,  C12N9/12

Abstract: A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.

公开(公告)号：US20190292591A1

公开(公告)日：2019-09-26

申请号：US16351456

申请日：2019-03-12

Applicant: GENIA TECHNOLOGIES, INC. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy GEISER ,  Randall DAVIS ,  Roger CHEN

IPC: C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.