利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

80 条记录 (耗时 0.045 秒)

    Apparatus and Methods for Self-Biasing Differential Signaling Circuitry Having Multimode Output Configurations for Low Voltage Applications
    72.
    发明申请
    Apparatus and Methods for Self-Biasing Differential Signaling Circuitry Having Multimode Output Configurations for Low Voltage Applications 有权
    具有用于低电压应用的多模输出配置的自偏压差分信号电路的装置和方法

    公开(公告)号：US20090115457A1

    公开(公告)日：2009-05-07

    申请号：US12352916

    申请日：2009-01-13

    申请人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard W. Fung

    发明人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard W. Fung

    IPC分类号： H03K19/20

    CPC分类号： H03K17/302 ,  H03K17/04106

    摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.

    摘要翻译： 本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。 两个或更多个开关被包括并且被配置为根据差分信号电路的操作模式来选择性地将电源电压耦合到输出。 电路还包括可操作地耦合到至少一个开关和差分信号电路的输出的开关控制偏置电路。 开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。 此外，体积偏置电路被包括并且可操作地耦合到开关。 体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

    UNSYMMETRICAL CYANINE DIMER COMPOUNDS AND THEIR APPLICATION
    74.
    发明申请
    UNSYMMETRICAL CYANINE DIMER COMPOUNDS AND THEIR APPLICATION 有权
    不对称氰基二异氰酸酯化合物及其应用

    公开(公告)号：US20080199875A1

    公开(公告)日：2008-08-21

    申请号：US12099020

    申请日：2008-04-07

    申请人： Stephen Yue ,  Ching-Ying Cheung

    发明人： Stephen Yue ,  Ching-Ying Cheung

    IPC分类号： C12Q1/68 ,  C07D401/14 ,  C07H21/00 ,  G01N21/76

    CPC分类号： C12Q1/6816 ,  Y10S436/80 ,  C12Q2563/173

    摘要： Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.

    摘要翻译： 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。

    Detection of immobilized nucleic acid
    78.
    发明申请
    Detection of immobilized nucleic acid 有权
    检测固定化核酸

    公开(公告)号：US20050239096A1

    公开(公告)日：2005-10-27

    申请号：US10956868

    申请日：2004-09-30

    申请人： Matthew Beaudet ,  W. Cox ,  Stephen Yue

    发明人： Matthew Beaudet ,  W. Cox ,  Stephen Yue

    IPC分类号： C07D413/02 ,  C07D417/02 ,  C07D417/06 ,  C09B23/02 ,  C12Q1/68 ,  C07D43/02

    CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

    摘要翻译： 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。 通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。 优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。 使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

    Cyanine compounds and their application as quenching compounds
    79.
    发明申请
    Cyanine compounds and their application as quenching compounds 有权
    花青化合物及其作为淬灭化合物的应用

    公开(公告)号：US20050191643A1

    公开(公告)日：2005-09-01

    申请号：US10916822

    申请日：2004-08-11

    申请人： Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    发明人： Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    IPC分类号： C07D209/12 ,  C07D209/24 ,  C07D401/04 ,  C07D471/02 ,  C07D491/02 ,  C07D498/02 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C07D209/12 ,  C07D209/14 ,  C07D209/24 ,  C07D401/04 ,  C09B23/12

    摘要： The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

    摘要翻译： 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。