摘要:
Embodiments of the invention provide a cap for a vessel for performing a multi-stage process for analyzing a sample, such as nested PCR or RT-PCR. In one embodiment, the cap comprises a body configured to be mated to the vessel to enclose a vessel interior, a cap cavity for holding reagents, and a cap cavity control portion that is adjustable with respect to the body between a first-stage position in which the cap cavity is enclosed and fluidicly isolated from the vessel interior and a second-stage position in which the cap cavity is fluidicly coupled with the vessel interior.
摘要:
The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
摘要:
A frequency synthesizer with a single PLL and multiple SSB mixers is presented. The frequency synthesizer includes a single PLL outputting a reference signal that is fed to a plurality of dividers coupled in sequence. The outputs from the dividers are mixed by the SSB mixers to produce signals with different frequencies. These signals with different frequencies can be selected through use of multiple selectors.
摘要:
Embodiments of the invention provide an efficient and effective technique for storing and dispensing reagent beads. In one embodiment, an apparatus is provided for dispensing reagent beads contained in a bead storage device which includes a bead carrier having a plurality of wells; a plurality of reagent beads disposed in the wells; and a cover tape releasably attached to the bead carrier to cover the wells and retain the reagent beads in the wells. The apparatus comprises a channel in which to place the bead storage device with the bead carrier facing a support wall of the channel and the cover tape facing a stripping wall of the channel. The stripping wall includes a stripping gap disposed between a stripping edge and an opposite edge, and a dispense opening provided adjacent the opposite edge on a side of the stripping wall opposite from the stripping edge. The cover tape is insertable through the stripping gap to be pulled against the stripping edge to peel the cover tape from the bead carrier to move the wells of the bead carrier inside the channel toward the dispense opening and expose the wells individually to dispense the reagent beads.
摘要:
Compounds disclosed herein are bradykinin B1 antagonist compounds useful in the treatment or prevention of symptoms such as pain and inflammation associated with the bradykinin B1 pathway.
摘要:
A valve for a needleless syringe fitting is disclosed. The valve has a receptacle fitting for releasable attachment to the syringe fitting, a valve chamber, a valve seat adjacent the chamber, and elastomeric sealing bowl within the chamber. The bowl is deformed and partially inverted when the syringe fitting is attached to the receptacle fitting, causing the valve to open. Also disclosed is a valved container in which a vial is sealed by a valve of this type.
摘要:
A track and hold circuit is disclosed which may be used in high speed analog to digital conversions. The circuit includes a control transistor which keeps the circuit's input transistor in a conductive state even when the circuit is in hold mode. As a result, the track and hold circuit achieves a high switching speed while minimizing input voltage spikes.
摘要:
The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
摘要:
A differential amplifier is provided in the present invention. The differential amplifier includes an amplifying module having a resistive ratio, receiving an input voltage, and amplifying the input voltage as an output voltage in accordance with the resistive ratio; and a feedback module coupled with the amplifying module and generating a feedback signal in accordance with the input voltage and the output voltage for regulating the output voltage.
摘要:
A driving circuit for driving a load is provided. The driving circuit includes a control module providing a control signal to adjust a load current flowing through the load; and a voltage clamping module providing a power voltage to the control module.