摘要:
The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
摘要:
Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译:分离的多核苷酸,其包含选自以下的多核苷酸序列:a)与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸,b)多核苷酸, 其编码多肽,其包含与SEQ ID No.2的氨基酸序列至少70%的程度相同的氨基酸序列,c)与a)或b)的多核苷酸互补的多核苷酸, 和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少lysR2基因存在于减毒 形式,以及使用多核苷酸序列作为杂交探针。
摘要:
An isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids by attenuation of the poxB gene.
摘要翻译:一种分离的多核苷酸,其含有多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码含有SEQ ID NO:1的氨基酸序列的多肽的多核苷酸至少70%相同。 2,b)编码多肽的多核苷酸,其含有与SEQ ID No:1的氨基酸序列至少70%相同的氨基酸序列。 2)c)与a)或b)的多核苷酸互补的多核苷酸,和d)包含a),b)或c)多核苷酸序列的至少15个连续碱基的多核苷酸,以及发酵生产L - 氨基酸通过减少poxB基因。
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the citB gene, and a host-vector system having a coryneform host bacterium in which the citB gene is present in attenuated form and a vector which carries at least the citB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码citB基因的多核苷酸序列的分离的多核苷酸,以及具有其中citB基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带citB基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the gorA gene, and a host-vector system having a coryneform host bacterium in which the gorA gene is present in attenuated form and a vector which carries at least the gorA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码gorA基因的多核苷酸序列的分离的多核苷酸,以及具有其中gorA基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带gorA基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The present invention relates lo an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group c insisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of(a) or (b), and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.
摘要翻译:本发明涉及一种来自谷氨酸棒杆菌的分离的多核苷酸,其包含选自下组的多核苷酸序列,所述多核苷酸序列坚持:(a)与编码多肽的多核苷酸的程度相同的多核苷酸,所述多核苷酸包含氨基 SEQ ID NO:2的酸序列; (b)编码多肽的多核苷酸,其包含与SEQ ID NO:2的氨基酸序列至少70%的程度相同的氨基酸序列; (c)与(a)或(b)的多核苷酸互补的多核苷酸,和(d)包含(a),(b)或(c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸, 以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少sigE基因以增强形式存在,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which. comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70% ,b)编码多肽的多核苷酸。 包括与SEQ ID No.2的氨基酸序列至少70%的程度相同的氨基酸序列,c)与a)或b)的多核苷酸互补的多核苷酸,和d)包含在 a),b)或c)的多核苷酸序列的至少15个连续核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少citA基因以减毒形式存在,以及使用 包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to polynucleotides that contain polynucleotide sequences coding for the genes sucC and sucD, selected from the group a) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 2, b) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 3, c) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No. 2, d) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No. 3, e) polynucleotide that is complementary to the polynucleotides of a), b), c) or d), and f) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b), c), d) or e), a process for the fermentative production of L-amino acids using coryneform bacteria in which the genes are present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译:本发明涉及含有编码基因sucC和sucD的多核苷酸序列的多核苷酸,其选自a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸 ,b)与编码含有SEQ ID No.3的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸,c)编码含有至少70%的氨基酸序列的多肽的多核苷酸, 与SEQ ID No.2的氨基酸序列相同,d)编码含有与SEQ ID No.3的氨基酸序列至少70%相同的氨基酸序列的多肽的多核苷酸,e) 与a),b),c)或d)的多核苷酸互补,以及f)包含a),b),c),d)或e)的多核苷酸序列的至少15个连续核苷酸的多核苷酸, 发酵生产 使用棒状细菌的L-氨基酸,其中基因以减毒形式存在,以及使用多核苷酸序列作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.
摘要翻译:本发明涉及来自棒状细菌的分离的多核苷酸,其含有至少一种多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码含有根据SEQ ID No.3所述的氨基酸序列的多肽的多核苷酸至少70%相同。 2,b)编码多肽的多核苷酸,其含有与SEQ ID NO:1的氨基酸序列至少70%相同的氨基酸序列。 2)c)与a)或b)的多核苷酸互补的多核苷酸,和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及d)发酵生产的方法 的L-氨基酸,特别是L-赖氨酸。
摘要:
The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been derived from Corynebacterium glutamicum. The invention also encompasses methods for fermentatively producing amino acids using bacteria in which phosphofructokinase activity has been enhanced