公开(公告)号：US20050279634A1

公开(公告)日：2005-12-22

申请号：US11213961

申请日：2005-08-30

申请人： Nobuhiko Ozaki ,  Hiroaki Oka

发明人： Nobuhiko Ozaki ,  Hiroaki Oka

IPC分类号： C12M1/34 ,  G01N33/483 ,  G01N7/00

CPC分类号： G01N33/48728

摘要： It is an object of the invention to provide an instrument for pharmacologic measurement capable of detecting a very small and short time change in electric signal caused by a pharmacologic action of a biologic specimen with quickness and a high sensitivity by steeply decreasing an external disturbance component mixed into the system while dropping/exchange of medicinal solutions is conducted in a batch mode. The pharmacologic measurement instrument 20 detects a change in electric signal caused by a pharmacologic action or electrophysiologic action of a biologic specimen, and includes: an electrically conductive box 2 having an opening section 8 at the top surface thereof; and a well vessel 19 disposed in the opening section 8 thereof, wherein the well vessel 19 includes: a base 1 having recesses 4 in which the biologic specimens 6 are put; measurement electrodes 10 formed on the bottom surfaces of the respective recesses 4; and reference electrodes 13 electrically insulated from the respective measurement electrodes 10, and the reference electrodes 13 together with the electrically conductive box 2 electrostatically shields the well vessel 19.

摘要翻译： 本发明的目的是提供一种用于药物测量的仪器，其能够通过快速和高灵敏度来检测由生物样本的药理作用引起的电信号的非常小的和短的时间变化，通过急剧地降低混合的外部干扰成分 在分批模式下进行药物溶液的滴加/交换时进入系统。 药理学测量仪器20检测由生物样本的药理作用或电生理作用引起的电信号变化，并且包括：导电盒2，其顶表面具有开口部分8; 以及设置在其开口部分8中的井管19，其中井筒19包括：具有凹部4的基部1，生物标本6放置在其中; 形成在各个凹部4的底面上的测量电极10; 并且与各测量电极10电绝缘的参考电极13以及参考电极13与导电盒2一起静电屏蔽井管19。

公开(公告)号：US20050274423A1

公开(公告)日：2005-12-15

申请号：US11195649

申请日：2005-08-03

申请人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Maki Katagiri

发明人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Maki Katagiri

IPC分类号： B01L3/00 ,  F15C1/04 ,  F15C5/00 ,  F16K99/00 ,  G01N27/447 ,  G01N37/00

CPC分类号： G01N27/44791 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0661 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0036 ,  F16K2099/0074 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/206 ,  Y10T137/2196 ,  Y10T137/2224

摘要： The present invention is a method of moving a fluid in a capillary using a capillary chip having a layer including a polymer composition and a capillary formed on the surface or inside of the aforementioned layer including a polymer composition, wherein the aforementioned capillary has a movement control part, and the aforementioned movement control part includes multiple and sequential opening/closing parts; the aforementioned opening/closing part blocks movement of the fluid that flows in the aforementioned capillary by increase in the volume of the aforementioned polymer composition to result in the closed state, while it permits movement of the fluid that flows in the aforementioned capillary by decrease in the volume of the aforementioned polymer composition to result in the open state; and the aforementioned method of moving the fluid includes a step (a) of switching the aforementioned multiple opening/closing parts from the open state to the closed state sequentially in a movement direction by changing the temperature of the aforementioned polymer composition in the aforementioned movement control part, thereby moving the fluid in the aforementioned capillary.

摘要翻译： 本发明是一种使用毛细管芯片的毛细管芯片移动流体的方法，所述毛细管芯片具有包含聚合物组合物和形成在包括聚合物组合物的上述层的表面或内部的毛细管的毛细管，其中上述毛细管具有移动控制 上述移动控制部分包括多个和顺序的打开/关闭部分; 上述打开/关闭部分通过增加上述聚合物组合物的体积来阻止在上述毛细管中流动的流体的运动，导致关闭状态，同时允许通过减少在上述毛细管中流动的流体的运动 上述聚合物组合物的体积导致打开状态; 并且上述移动流体的方法包括：通过在上述移动控制中改变上述聚合物组合物的温度，将上述多个开闭部从打开状态向移动方向顺序地切换到闭合状态的步骤（a） 从而使上述毛细管中的流体移动。

公开(公告)号：US20050221469A1

公开(公告)日：2005-10-06

申请号：US10513392

申请日：2004-03-08

申请人： Masaya Nakatani ,  Hiroaki Oka ,  Fumiaki Emoto

发明人： Masaya Nakatani ,  Hiroaki Oka ,  Fumiaki Emoto

IPC分类号： G01N33/487 ,  C12M1/34

CPC分类号： G01N33/48728

摘要： An extracellular potential measuring device includes a plate portion having a first surface and a second surface opposite to the first surface, and an electrode provided on the second surface of the plate portion. In the plate portion, a pocket having an opening which opens to the first surface is formed, and a through-hole communicating to the second surface from the pocket. The through-hole communicates from a position which is closer to the opening than a deepest point of the first pocket. The electrode is provided around of the opening of the through-hole. In this device, even if a cell to be examined does not reach the deepest point of the pocket, a cell membrane of the cell can tightly attaches onto the through-hole securely without a clearance. Hence, culture solution inside the through-hole is isolated from culture solution over an upper surface of the plate portion, thereby allowing electrochemical changes caused by activities of the cell to be detected efficiently with a detector electrode.

摘要翻译： 细胞外电位测量装置包括具有第一表面和与第一表面相对的第二表面的板部分，以及设置在板部分的第二表面上的电极。 在该板部分中，形成具有向第一表面开口的开口的口袋，以及从口袋连通到第二表面的通孔。 通孔从比第一口袋的最深点更靠近开口的位置连通。 电极设置在通孔的开口周围。 在该装置中，即使要检查的细胞未达到口袋的最深点，细胞的细胞膜可以牢固地紧贴在通孔上而没有间隙。 因此，通孔内部的培养液在培养液的上表面上与培养液分离，从而能够通过检测器电极有效地检测细胞活性引起的电化学变化。

公开(公告)号：US20050032075A1

公开(公告)日：2005-02-10

申请号：US10674787

申请日：2003-10-01

申请人： Hidenobu Yaku ,  Tetsuo Yukimasa ,  Hiroaki Oka

发明人： Hidenobu Yaku ,  Tetsuo Yukimasa ,  Hiroaki Oka

IPC分类号： C12Q1/25 ,  C12Q1/68 ,  G01N33/00

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2521/543

摘要： Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H+ hardly permeable membrane having H+-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H+ concentration of at least either one of the solution at the front face side of the H+ hardly permeable membrane or the solution at the back face side of the H+ hardly permeable membrane, in a state where the H+-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).

摘要翻译： 提供了用于区分核酸的碱基序列中的碱基类型的方便的技术。 该技术包括制备含有核酸的样品溶液的步骤（a），具有与核酸互补结合的互补结合区的碱基序列的引物和核苷酸; 在引起引物延伸反应的条件下使样品溶液静置的步骤（b），在引起延伸反应时产生焦磷酸盐; 使样品溶液与H + - 焦磷酸酶的前端面从H + - 焦磷酸酶的前面向后表面接触的步骤（c），其中水解焦磷酸盐的活性位点 暴露在前面; 在H +难以渗透的膜的前面侧的溶液或H +的背面侧的溶液中的至少任一溶液的H +浓度的测定步骤（d） 在其中将H + - 焦磷酸酶浸入溶液中的状态下， 基于步骤（d）中的测量结果检测延伸反应的步骤（e）; 以及基于步骤（e）中的检测结果来区分核酸碱基序列中的碱基类型的步骤（f）。

公开(公告)号：US20050004442A1

公开(公告)日：2005-01-06

申请号：US10495621

申请日：2002-11-19

申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Hirokazu Sugihara

发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Hirokazu Sugihara

IPC分类号： B01L3/00 ,  G01N33/487 ,  A61B5/05

CPC分类号： G01N33/48707 ,  B01L3/5027

摘要： A measurement device 10 includes a measurement cell 11. The measurement cell 11 includes a substrates 17 and a substrate 16 formed on the substrate 17. The substrates 16 and 17 are bonded to each other. Thus, a flow pass 9 is formed in the measurement cell 11. As shown in FIG. 3, on the substrate 17 of the measurement cell 11, provided are a concave well 19, a protruding microelectrode 1 connected to a terminal 4a and formed on a side wall surface of the well 19, communicating tubes 15a and 15b connected to both of side surfaces of the well 19 (i.e., the flow pass 9) to face to each other, resistance baths 6a and 6b connected to the communicating tubes 15a and 15b, thin film resistors 3a and 3b arranged in the resistance baths 6a and 6b, and a reference electrode 2 connected to a terminal 4b.

摘要翻译： 测量装置10包括测量单元11.测量单元11包括基板17和形成在基板17上的基板16.基板16和17彼此接合。 因此，在测量单元11中形成流路9。 如图3所示，在测量单元11的基板17上设置凹槽19，连接到端子4a并形成在井19的侧壁表面上的突出微电极1，连接到两侧的连通管15a和15b 孔19的表面（即，流路9）彼此相对，连接到连通管15a和15b的电阻槽6a和6b，布置在电阻槽6a和6b中的薄膜电阻器3a和3b，以及 参考电极2连接到端子4b。

公开(公告)号：US20130010300A1

公开(公告)日：2013-01-10

申请号：US13613325

申请日：2012-09-13

申请人： Masaya Tamura ,  Hiroshi Kagata ,  Hiroaki Oka

发明人： Masaya Tamura ,  Hiroshi Kagata ,  Hiroaki Oka

IPC分类号： G01N21/55 ,  B05D5/06

CPC分类号： G01N21/553

摘要： A plasmon sensor includes a first metal layer and a second metal layer having an upper surface facing a lower surface of the first metal layer. The upper surface of the first metal layer is configured to receive an electromagnetic wave. A hollow space is provided between the first and second metal layers, and is configured to be filled with a test sample containing a medium. This plasmon sensor has a small size and a simple structure.

摘要翻译： 等离子体激元传感器包括第一金属层和具有面向第一金属层的下表面的上表面的第二金属层。 第一金属层的上表面被配置为接收电磁波。 在第一和第二金属层之间设置中空空间，并且被配置为填充含有介质的测试样品。 该等离子体传感器具有体积小且结构简单的特点。

公开(公告)号：US08257962B2

公开(公告)日：2012-09-04

申请号：US10513392

申请日：2004-03-08

申请人： Masaya Nakatani ,  Hiroaki Oka ,  Fumiaki Emoto

发明人： Masaya Nakatani ,  Hiroaki Oka ,  Fumiaki Emoto

IPC分类号： C12M1/42 ,  C12M3/00 ,  C12N13/00

CPC分类号： G01N33/48728

摘要： An extracellular potential measuring device includes a plate portion having a first surface and a second surface opposite to the first surface, and an electrode provided on the second surface of the plate portion. In the plate portion, a pocket having an opening which opens to the first surface is formed, and a through-hole communicating to the second surface from the pocket. The through-hole communicates from a position which is closer to the opening than a deepest point of the first pocket. The electrode is provided around of the opening of the through-hole. In this device, even if a cell to be examined does not reach the deepest point of the pocket, a cell membrane of the cell can tightly attaches onto the through-hole securely without a clearance. Hence, culture solution inside the through-hole is isolated from culture solution over an upper surface of the plate portion, thereby allowing electrochemical changes caused by activities of the cell to be detected efficiently with a detector electrode.

摘要翻译： 细胞外电位测量装置包括具有第一表面和与第一表面相对的第二表面的板部分，以及设置在板部分的第二表面上的电极。 在该板部分中，形成具有向第一表面开口的开口的口袋，以及从口袋连通到第二表面的通孔。 通孔从比第一口袋的最深点更靠近开口的位置连通。 电极设置在通孔的开口周围。 在该装置中，即使要检查的细胞未达到口袋的最深点，细胞的细胞膜可以牢固地紧贴在通孔上而没有间隙。 因此，通孔内部的培养液在培养液的上表面上与培养液分离，从而能够通过检测器电极有效地检测细胞活性引起的电化学变化。

公开(公告)号：US08202687B2

公开(公告)日：2012-06-19

申请号：US13248880

申请日：2011-09-29

申请人： Masato Suzuki ,  Hiroaki Oka ,  Shigeki Kiyonaka ,  Yasuo Mori

发明人： Masato Suzuki ,  Hiroaki Oka ,  Shigeki Kiyonaka ,  Yasuo Mori

IPC分类号： C12Q1/00 ,  G01N33/53

CPC分类号： G01N33/566 ,  G01N33/6872

摘要： An object of the present invention is to detect a specific chemical substance with high sensitivity and high precision. By using a specific lipid bilayer membrane, a chemical substance is detected with high sensitivity and high precision. Here, the specific lipid bilayer membrane comprises a chemical substance receptor, a chimeric G protein, and a potassium ion channel. The chimeric G protein comprises a chimeric Gα subunit and a Gβγ subunit complex. The chimeric Gα subunit is selected from the group consisting of Gi/olf13 (SEQ ID NO: 04), Gi/olf28 (SEQ ID NO: 05), Gi/olf94 (SEQ ID NO: 07), Gi/olf113 (SEQ ID NO: 08), Gi/olf α3-β5,C (SEQ ID NO: 12), and Gi/olfα4-β6,C (SEQ ID NO: 15).

摘要翻译： 本发明的目的是检测具有高灵敏度和高精度的特定化学物质。 通过使用特定的脂质双层膜，以高灵敏度和高精度检测化学物质。 这里，特定脂质双层膜包含化学物质受体，嵌合G蛋白和钾离子通道。 嵌合G蛋白包含嵌合Gα亚基和G＆bgrγγ亚基复合物。 嵌合Gα亚基选自Gi / olf13（SEQ ID NO：04），Gi / olf28（SEQ ID NO：05），Gi / olf94（SEQ ID NO：07），Gi / olf113（SEQ ID NO： NO：08），Gi /olfα3-和bgr; 5，C（SEQ ID NO：12）和Gi /olfα4-＆bgr; 6，C（SEQ ID NO：15）。

公开(公告)号：US08202439B2

公开(公告)日：2012-06-19

申请号：US12359426

申请日：2009-01-26

申请人： Masaya Nakatani ,  Soichiro Hiraoka ,  Hiroshi Ushio ,  Akiyoshi Oshima ,  Hiroaki Oka ,  Fumiaki Emoto

发明人： Masaya Nakatani ,  Soichiro Hiraoka ,  Hiroshi Ushio ,  Akiyoshi Oshima ,  Hiroaki Oka ,  Fumiaki Emoto

IPC分类号： B44C1/22 ,  H01L21/302

CPC分类号： G01N33/48728 ,  Y10T428/24322

摘要： A diaphragm is formed by etching a substrate. This substrate has a first surface provided with a depression by isotropic dry etching, and a second surface opposite the first surface. Furthermore, a through-hole is formed from the depression to the second surface by anisotropic dry etching. The depression and the through-hole are formed by using one resist mask. The depression has a hemispherical shape or a semi-elliptical spherical shape.

摘要翻译： 通过蚀刻基板形成隔膜。 该基板具有通过各向同性干蚀刻设置有凹部的第一表面和与第一表面相对的第二表面。 此外，通过各向异性干蚀刻从凹陷形成通孔到第二表面。 通过使用一个抗蚀剂掩模形成凹陷和通孔。 凹陷具有半球形或半椭圆形球形。

公开(公告)号：US07857963B2

公开(公告)日：2010-12-28

申请号：US12683502

申请日：2010-01-07

申请人： Hidehiro Sasaki ,  Hiroaki Oka

发明人： Hidehiro Sasaki ,  Hiroaki Oka

IPC分类号： G01N27/30

CPC分类号： G01N27/3271

摘要： To provide an electrode plate for electrochemical measurements that enables detecting and quantifying the concentration of a target substance contained in a sample solution with rapidity and favorable sensitivity using an apparatus for electrochemical measurements is objected to.Specifically, the present invention is directed to a method of the determination using an electrode plate for electrochemical measurements 1 including a substrate 31, an upper layer 40 provided on the upper face of the substrate, a lower layer 11 provided on the lower face of the substrate, a first electrode body 32 sandwiched between the upper face of the substrate and the upper layer, and a second electrode body 12 sandwiched between the lower face of the substrate and the lower layer, wherein: the upper layer has a plurality of upper layer through-holes 41a and 41b; the first electrode body has a plurality of first electrodes 32d including a portion exposed from the upper face of the upper layer via the upper layer through-hole 41b; the substrate has a plurality of substrate through-holes 33; and the second electrode body has a plurality of second electrodes 12d including a portion exposed from the upper face of the upper layer via the upper layer through-hole 41a and the substrate through-hole in the second electrode body.

摘要翻译： 为了提供用于电化学测量的电极板，可以使用电化学测量装置来检测和定量检测和定量样品溶液中包含的目标物质的浓度，并以快速和良好的灵敏度。 具体而言，本发明涉及一种使用电化学测定用电极板1进行测定的方法，所述电极板包括基板31，设置在基板的上表面上的上层40，设置在基板31的下表面上的下层11 基板，夹在基板的上表面和上层之间的第一电极体32和夹在基板的下表面和下层之间的第二电极体12，其中：上层具有多个上层 通孔41a和41b; 第一电极体具有经由上层通孔41b从上层的上表面露出的部分的多个第一电极32d; 基板具有多个基板通孔33; 并且第二电极体具有多个第二电极12d，第二电极12d包括经由上层通孔41a和第二电极体中的基板通孔从上层的上表面露出的部分。