公开(公告)号：US4780879A

公开(公告)日：1988-10-25

申请号：US16410

申请日：1987-02-19

申请人： Naoki Chinone ,  Shigeo Yamashita ,  Shin'ichi Nakatsuka ,  Akemi Yamanaka ,  Yuichi Ono ,  Toshihiro Kawano ,  Kazuhisa Uomi ,  Takashi Kajimura ,  Toshiaki Tanaka ,  Kunio Aiki

发明人： Naoki Chinone ,  Shigeo Yamashita ,  Shin'ichi Nakatsuka ,  Akemi Yamanaka ,  Yuichi Ono ,  Toshihiro Kawano ,  Kazuhisa Uomi ,  Takashi Kajimura ,  Toshiaki Tanaka ,  Kunio Aiki

IPC分类号： H01S5/00 ,  H01S3/00 ,  H01S5/026 ,  H01S5/10 ,  H01S3/19

CPC分类号： H01S5/1082 ,  H01S5/0267 ,  H01S5/1064

摘要： A semiconductor laser device having a stripe-shaped active region defined between a pair of end surfaces, at least one of the end surfaces having a curved cross-section in a plane parallel to the active region with a radius of curvature from 10 to 300 .mu.m, the stripe of the active region having such a width that a single transverse mode and a multi-longitudinal mode are allowed. The laser beam emitted from this laser can have little astigmatism and small spot size.

摘要翻译： 一种半导体激光器件，其具有限定在一对端面之间的条状有源区域，所述端面中的至少一个在平行于有源区域的平面中具有弯曲的横截面，其曲率半径为10至300μm m，有源区域的条纹具有允许单横模和多纵模的宽度。 从该激光器发射的激光束可以具有很少的散光和小的斑点尺寸。

公开(公告)号：US09453840B2

公开(公告)日：2016-09-27

申请号：US14234361

申请日：2012-07-27

申请人： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Yuichi Ono

发明人： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Yuichi Ono

IPC分类号： C12N15/08 ,  G01N33/569 ,  C12N5/0793 ,  C12N5/0797

CPC分类号： G01N33/56966 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/727 ,  C12N2502/1394 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00

摘要： The present invention provides a method for selecting dopaminergic neuron progenitor cells, which comprises detecting any one or more of markers selected from the group consisting of CD15 (SSEA-1), CD24, CD46, CD47, CD49b, CD57, CD58, CD59, CD81, CD90, CD98, CD147, CD184, Disalogangliosid GD2, SSEA-4, CD49f, SERINC4, CCR9, PHEX, TMPRSS11E, HTR1E, SLC25A2, Ctxn3, Ccl7, Chrnb4, Chrna3, Kcnv2, Grm2, Syt2, Lim2, Mboat1, St3gal6, Slc39a12, Tacr1, Lrtm1, Dscam and CD201.

摘要翻译： 本发明提供了选择多巴胺能神经元祖细胞的方法，其包括检测选自CD15（SSEA-1），CD24，CD46，CD47，CD49b，CD57，CD58，CD59，CD81的任何一种或多种标志物 ，CD90，CD98，CD147，CD184，Disalogangliosid GD2，SSEA-4，CD49f，SERINC4，CCR9，PHEX，TMPRSS11E，HTR1E，SLC25A2，Ctxn3，Ccl7，Chrnb4，Chrna3，Kcnv2，Grm2，Syt2，Lim2，Mboat1，St3gal6， Slc39a12，Tacr1，Lrtm1，Dscam和CD201。

公开(公告)号：US20110256548A1

公开(公告)日：2011-10-20

申请号：US13141063

申请日：2009-12-18

申请人： Yuichi Ono ,  Tomoya Nakatani ,  Yasuko Nakagawa

发明人： Yuichi Ono ,  Tomoya Nakatani ,  Yasuko Nakagawa

IPC分类号： C12Q1/68 ,  C12N5/071 ,  G01N33/567

CPC分类号： C12N5/0678

摘要： The present invention provides markers that can selectively distinguish pancreatic progenitor cells. The present invention also provides methods for distinguishing pancreatic progenitor cells by using the markers as an indicator, and reagents to be used in the methods.The present inventors successfully identified the surface marker Nephrin-like 3 (Neph3) which is specifically expressed in pancreatic progenitor cells, and isolated pancreatic progenitor cells using the marker as an indicator or such. Viable pancreatic progenitor cells can be selected by using Neph3 as an indicator without using translated products and transcripts of any foreign genes. The marker is useful in preparing and identifying pancreatic progenitor cells which are applied to regenerative medicine or such for treatment of pancreatic diseases.

摘要翻译： 本发明提供了可以选择性区分胰腺祖细胞的标志物。 本发明还提供了通过使用标记作为指标来区分胰腺祖细胞的方法，以及用于该方法的试剂。 本发明人成功地鉴定了在胰腺祖细胞中特异性表达的表面标记物Nephrin样3（Neph3），以及使用该标记作为指示剂的分离的胰腺祖细胞。 可以通过使用Neph3作为指标来选择可行的胰腺祖细胞，而不使用翻译的产物和任何外来基因的转录物。 该标记物可用于制备和鉴定适用于再生医学或胰腺疾病治疗的胰腺祖细胞。

公开(公告)号：US20100303771A1

公开(公告)日：2010-12-02

申请号：US12524153

申请日：2008-02-07

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Eri Mizuhara

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Eri Mizuhara

IPC分类号： A61K35/30 ,  C12Q1/68 ,  G01N33/68 ,  G01N33/567 ,  C12N5/0797 ,  C07H21/00 ,  C07K16/18 ,  C07H21/04 ,  A61P25/00 ,  A61P25/28

CPC分类号： C07K16/40 ,  C07K14/47 ,  C07K16/18 ,  G01N33/5023 ,  G01N33/5058 ,  G01N33/5073 ,  G01N33/56966 ,  G01N33/9426

摘要： The present inventors identified a selective marker 65B13 for GABA neuron progenitor cells of the spinal dorsal horn and cerebellum, and successfully isolated GABA neuron progenitor cells using antibodies that bind to a protein encoded by the gene. 65B13 was demonstrated to be useful as a marker to isolate GABA-producing neuron progenitor cells in the spinal dorsal horn and cerebellum. GABA neuron progenitor cells can be efficiently identified or isolated by using the identified marker as an indicator.

摘要翻译： 本发明人鉴定了用于脊髓背角和小脑的GABA神经元祖细胞的选择性标记65B13，并且使用结合由该基因编码的蛋白质的抗体成功分离GABA神经元祖细胞。 65B13被证明可用作分离脊髓背角和小脑中产生GABA的神经元祖细胞的标志物。 通过使用鉴定的标记作为指标，可以有效地鉴定或分离GABA神经元祖细胞。

公开(公告)号：US07807371B2

公开(公告)日：2010-10-05

申请号：US12110111

申请日：2008-04-25

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Yoshimasa Sakamoto

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such.According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.

摘要翻译： 在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。 此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。 根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。 在细胞中使用Lrp4表达作为指标，可以进行分离。 的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。

公开(公告)号：US20100028866A1

公开(公告)日：2010-02-04

申请号：US12064018

申请日：2006-08-18

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani ,  Yoshimasa Sakamoto

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07K16/00 ,  C12N5/02

CPC分类号： C07K16/18 ,  C07K14/47 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5073

摘要： The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of a Nato3 gene, or a complementary sequence thereto, and an antibody against a Nato3 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.

摘要翻译： 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可与由Nato3基因的核苷酸序列或互补的多核苷酸组成的多核苷酸杂交 序列，以及针对Nato3蛋白或其部分的抗体用于检测或选择多巴胺能神经元增殖祖细胞。

公开(公告)号：US07622270B2

公开(公告)日：2009-11-24

申请号：US10532264

申请日：2003-10-21

申请人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshimasa Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

发明人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshimasa Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

IPC分类号： G01N33/567 ,  C07K16/28

CPC分类号： G01N33/6896 ,  C07K14/47

摘要： A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.

摘要翻译： 通过本发明获得在细胞周期出口后立即在多巴胺能神经元前体细胞中特异性和瞬时表达的新基因65B13。 65B13的细胞表达可用作选择适合于其安全性，存活率和网络形成能力的细胞的指标，用于神经变性疾病如帕金森病的移植治疗。

公开(公告)号：US20080213757A1

公开(公告)日：2008-09-04

申请号：US11574115

申请日：2005-08-23

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

IPC分类号： C12Q1/68 ,  G01N33/567

CPC分类号： C12Q1/6876 ,  C12Q2600/158 ,  G01N33/5026 ,  G01N33/5058 ,  G01N33/5073

摘要： As a result of screening for genes that are selectively expressed in fetal mouse brain region by subtraction method, the present inventors obtained a cDNA fragment encoding Corl1. The expression of Corl1 was examined by RT-PCR, in situ hybridization, and immunostaining using polyclonal antibodies. The results demonstrated that Corl1 was especially expressed at a high level of selectively in the central nervous system during embryonic stages. The expression patterns of Corl1 determined using various markers in embryonic spinal cord were compared to identify types of neurons expressing Corl1. The results revealed that Corl1 was specifically expressed in spinal cord interneurons dI4, dI5, dILA, and dILB. Accordingly, the present invention provides for discrimination between dI4 and dI6, neurons which previously could only be discriminated based on developmental location, using the expression of Corl1 as an indicator.

摘要翻译： 作为筛选通过减法法在胎儿小鼠脑区域中选择性表达的基因的结果，本发明人获得了编码Corl1的cDNA片段。 通过RT-PCR，原位杂交和使用多克隆抗体的免疫染色检测Corl1的表达。 结果表明，Corl1在胚胎期期间在中枢神经系统的高水平选择性表达。 比较使用胚胎脊髓中各种标记物测定的Corl1的表达模式，以鉴定表达Corl1的神经元的类型。 结果表明，Corl1在脊髓中间神经元dI4，dI5，dILA和dILB中特异性表达。 因此，本发明提供了使用Corl1的表达作为指标的dI4和dI6之间的区别，以前仅可以基于发育位置鉴别神经元。

公开(公告)号：US20080078679A1

公开(公告)日：2008-04-03

申请号：US11576887

申请日：2005-10-05

申请人： Masanori Yamaguchi ,  Yuichi Ono ,  Susumu Kosemura ,  Eiji Nishimura ,  Tadashi Ogasawara ,  Makoto Yamaguchi ,  Masahiko Hori ,  Toru Uenishi

发明人： Masanori Yamaguchi ,  Yuichi Ono ,  Susumu Kosemura ,  Eiji Nishimura ,  Tadashi Ogasawara ,  Makoto Yamaguchi ,  Masahiko Hori ,  Toru Uenishi

IPC分类号： C25C3/02 ,  C25C3/28 ,  C25C7/00

CPC分类号： C22B34/129 ,  C25C3/02 ,  C25C3/28 ,  C25C7/025

摘要： A method for production of metal by molten-salt electrolysis of the present invention is a method for production of metal by molten-salt electrolysis which is performed by filling a molten salt of calcium chloride in an electrolysis vessel having a anode and a cathode, one of the anode or cathode is arranged surrounding the other electrode, the cathode has at least one hole communicating the inner area surrounded by the cathode with the outer area, and the molten salt flows through the communicating holes from one area including the anode (the inner area or outer area) to the other area.

摘要翻译： 本发明的通过熔盐电解生产金属的方法是通过熔融盐电解生产金属的方法，其通过在具有阳极和阴极的电解容器中填充氯化钙的熔融盐，一种 阳极或阴极被布置为围绕另一电极，阴极具有至少一个孔，其将由阴极包围的内部区域与外部区域连通，并且熔融盐从包括阳极的一个区域（内部）流过连通孔 区域或外部区域）到另一个区域。

公开(公告)号：US5101949A

公开(公告)日：1992-04-07

申请号：US674772

申请日：1991-03-25

申请人： Kazunori Takikawa ,  Yuichi Ono ,  Hiroshi Inoue

发明人： Kazunori Takikawa ,  Yuichi Ono ,  Hiroshi Inoue

IPC分类号： F01P7/08 ,  F16D35/00 ,  F16D35/02

CPC分类号： F16D35/022

摘要： A temperature sensitive fluid-type fan coupling device comprises a closed enclosure including a casing journalled on a rotary shaft through a bearing and having a cooling fan at its outer periphery, and a cover, a partition adapted to divide the interior of the closed enclosure into an oil chamber and a torque transmission chamber within which a drive disk is located and including a oil flow adjustment opening, a dam mounted to the inner periphery of the closed enclosure in a face-to-face relation to the outer periphery of the drive disk, an oil flowing toward the inner periphery of the closed enclosure during spinning of the closed enclosure, a circulation passage extending from the torque transmission chamber to the oil chamber through the dam, a valve element mounted within the oil chamber and having one end operable to open the flow adjustment opening when external temperature exceeds a given temperature and close the flow adjustment opening when external temperature is below the given temperature, a temperature sensitive element mounted to the front of the cover and deformed according to temperature fluctuation to move the valve element, and a magnet mounted to bias the valve element in a direction to open the flow adjustment opening.

摘要翻译： 温度敏感型流体式风扇联接装置包括封闭的外壳，其包括通过轴承支承在旋转轴上并在其外周边具有冷却风扇的外壳，以及盖，适于将封闭外壳的内部分成 油室和扭矩传递室，驱动盘位于该油室和转矩传递室内，并且包括油流调节开口，与驱动盘的外周面对面地安装到封闭壳体的内周的坝 在封闭的外壳旋转期间流向封闭外壳的内周的油，从扭矩传递室通过坝延伸到油室的循环通道，安装在油室内的阀元件，其一端可操作以 当外部温度超过给定温度时打开流量调节开口，当外部温度低于g时关闭流量调节开口 温度敏感元件，安装在盖的前部并根据温度波动而变形以移动阀元件;以及磁体，其安装成沿着打开流量调节开口的方向偏压阀元件。