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272 条记录 (耗时 0.049 秒)

    Anti-rhesus d recombinant polyclonal antibody and methods of manufacture
    71.
    发明申请
    Anti-rhesus d recombinant polyclonal antibody and methods of manufacture 失效
    抗恒河猴重组多克隆抗体及其制备方法

    公开(公告)号：US20090017017A1

    公开(公告)日：2009-01-15

    申请号：US11632937

    申请日：2005-07-18

    申请人： Soren Kofoed Rasmussen ,  Anne Bondgaard Tolstrup ,  Soren Bregenholt Frederiksen ,  John Haurum

    发明人： Soren Kofoed Rasmussen ,  Anne Bondgaard Tolstrup ,  Soren Bregenholt Frederiksen ,  John Haurum

    IPC分类号： A61K39/395 ,  C40B30/06 ,  C12N5/06 ,  C12P21/04 ,  A61P7/00 ,  C40B40/08 ,  C07K16/18

    CPC分类号： C07K16/34 ,  C07K16/005 ,  C07K2317/21 ,  C07K2317/55 ,  C07K2317/77

    摘要： The invention relates to a method for manufacturing an anti-RhD recombinant polyclonal antibody composition (anti-RhD rpAb). The method comprises obtaining a collection of cells transfected with a library of anti-RhD antibody expression vectors, wherein each cell in the collection is capable of expressing from a VH and VL comprising nucleic acid segment, one member of the library, which encodes a distinct member of anti-RhD recombinant polyclonal antibody composition and which is located at the same site in the genome of individual cells in said collection. The cells are cultured under suitable conditions for expression of the recombinant polyclonal antibody, which is obtained from the cells or culture supernatant. The nucleic acid segments encoding the anti-RhD rpAb is introduced into the cells by transfection with a library of vectors for site-specific integration. The present method is suitable for manufacturing anti-RhD rpAb, thereby making available a superior replacement of plasma-derived prophylactic and therapeutic immonoglobulin products.

    摘要翻译： 本发明涉及一种制备抗RhD重组多克隆抗体组合物（抗RhD rpAb）的方法。 该方法包括获得用抗RhD抗体表达载体文库转染的细胞的集合，其中集合中的每个细胞能够从包含核酸片段的VH和VL表达，文库的一个成员编码不同的 抗RhD重组多克隆抗体组合物的成员，并且位于所述收集物中各个细胞的基因组中的相同位点。 在合适的条件下培养细胞以从细胞或培养上清液获得的重组多克隆抗体的表达。 编码抗RhD rpAb的核酸片段通过用载体文库转染引入细胞，用于位点特异性整合。 本发明方法适用于制备抗RhD rpAb，从而使得等离子体衍生的预防和治疗性亚铁蛋白产品更具优势。

    Capture, Concentration And Quantitation Of Abnormal Prion Protein From Biological Fluids Using Depth Filtration
    76.
    发明申请
    Capture, Concentration And Quantitation Of Abnormal Prion Protein From Biological Fluids Using Depth Filtration 审中-公开
    使用深度过滤从生物液体捕获，浓缩和定量异常朊病毒蛋白

    公开(公告)号：US20070148685A1

    公开(公告)日：2007-06-28

    申请号：US11674368

    申请日：2007-02-13

    申请人： Robert Van Holten ,  Stephen Autenrieth

    发明人： Robert Van Holten ,  Stephen Autenrieth

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C07K16/065 ,  A23J3/12 ,  A23L5/20 ,  A23L5/23 ,  A61L2/0017 ,  C07K1/34 ,  C07K16/34

    摘要： Methods for producing biological solutions such as immunoglobulins and in particular anti-D immunoglobulin substantially free of abnormal prion protein resulting therefrom. Specifically provided are methods for aggregation of prions and depth filtration of the biological solution to capture and remove abnormal and if desired, normal prion protein. The prion protein may then be eluted from the depth filter and filter washes and concentrated sufficient for detection at limits currently required by available assays.

    摘要翻译： 用于生产生物溶液如免疫球蛋白的方法，特别是基本上不含由其产生的异常朊病毒蛋白的抗-D免疫球蛋白。 具体提供了用于聚集生物溶液的朊病毒和深度过滤以捕获和除去异常和如果需要的正常朊病毒蛋白的方法。 然后可以从深度过滤器洗脱朊蛋白，并将滤液洗涤并浓缩至足以在现有测定法所要求的限度下进行检测。

    Novel nucleic acid molecules correlated with the rhesus weak D phenotype
    78.
    发明申请
    Novel nucleic acid molecules correlated with the rhesus weak D phenotype 有权
    新型核酸分子与恒河猴弱D表型相关

    公开(公告)号：US20060104987A1

    公开(公告)日：2006-05-18

    申请号：US11200624

    申请日：2005-08-10

    申请人： Willy Flegel ,  Franz Wagner

    发明人： Willy Flegel ,  Franz Wagner

    IPC分类号： A61K39/00 ,  C12Q1/68 ,  G01N33/567 ,  C07H21/04 ,  C07K16/28 ,  C07K14/74

    CPC分类号： G01N33/6854 ,  C07K14/47 ,  C07K16/34 ,  G01N33/80

    摘要： The present invention relates to novel nucleic acid molecules encoding a Rhesus D antigen contributing to the weak D phenotype which are characterized by one or a combination of missense mutations or by a gene conversion involving exons 6 to 9 of the RHD and RHCE genes. The present invention further relates to vectors comprising the nucleic acid molecules of the invention, to hosts transformed with said vectors, to proteins encoded by said nucleic acid molecules and to methods of producing such polypeptides. The fact that missense mutations and the conversion referred to above can be directly correlated to the weak D phenotype has a significant impact on the routine testing of blood samples. For example, oligonucleotides and antibodies can now be designed that generally allow the detection of weak D phenotypes in a sample. Such oligonucleotides, antibodies as well as a variety of diagnostic methods all fall within the scope of the present invention. RhD antigens encoded by the novel nucleic acid molecules may be used for the characterization, standardization and quality control of monoclonal and polyclonal anti-D antisera. Finally, the invention relates to a kit useful for testing for the presence of weak D phenotypes.

    摘要翻译： 本发明涉及编码有助于弱D表型的恒河猴D抗原的新型核酸分子，其特征在于一种或多种错义突变的组合或通过涉及RHD和RHCE基因的外显子6至9的基因转化。 本发明还涉及包含本发明的核酸分子的载体，用所述载体转化的宿主，由所述核酸分子编码的蛋白质以及产生这种多肽的方法。 事实上，错义突变和上述转化可以与弱D表型直接相关，对血液样本的常规检测有显着影响。 例如，现在可以设计寡核苷酸和抗体，其通常允许检测样品中的弱D表型。 这样的寡核苷酸，抗体以及各种诊断方法都落入本发明的范围内。 由新型核酸分子编码的RhD抗原可用于单克隆抗体和多克隆抗-D抗血清的表征，标准化和质量控制。 最后，本发明涉及可用于测试弱D表型存在的试剂盒。

    Rh blood group antigen compositions and methods of use
    80.
    发明授权
    Rh blood group antigen compositions and methods of use 失效
    Rh血型抗原组合物及使用方法

    公开(公告)号：US06911178B2

    公开(公告)日：2005-06-28

    申请号：US09991121

    申请日：2001-11-14

    申请人： L. Scott Rodkey ,  Marwan A. Yared ,  Kenneth J. Moise, Jr.

    发明人： L. Scott Rodkey ,  Marwan A. Yared ,  Kenneth J. Moise, Jr.

    IPC分类号： A61K35/14 ,  A61K38/00 ,  C07K14/705 ,  C07K16/34 ,  G01N33/80 ,  G01N33/00

    CPC分类号： G01N33/80 ,  A61K38/00 ,  C07K14/705 ,  C07K16/34 ,  Y10S435/975 ,  Y10S436/824 ,  Y10T436/10

    摘要： Disclosed are novel protein and peptide compositions comprising soluble and bound forms of immunologically-active blood group antigens including mammalian Rh antigens. In preferred embodiments methods for the isolation and purification of serologically-active human Rh antigens such as D, c, C, E, and e are disclosed. Also disclosed are methods for the adsorption of immunologically-active Rh antigens to solid supports. Diagnostic kits, methods, and devices for the detection of Rh antibodies in clinical and non-clinical samples are also disclosed. Devices, compositions and methods for the isolation, purification and quantitation of anti-Rh antibodies from solution are also provided.

    摘要翻译： 公开了包含可溶性和结合形式的免疫活性血型抗原（包括哺乳动物Rh抗原）的新型蛋白质和肽组合物。 在优选实施方案中，公开了用于分离和纯化血清学活性的人Rh抗原如D，c，C，E和e的方法。 还公开了用于将免疫活性Rh抗原吸附到固体支持物上的方法。 还公开了用于检测临床和非临床样品中Rh抗体的诊断试剂盒，方法和装置。 还提供了用于从溶液中分离，纯化和定量抗Rh抗体的装置，组合物和方法。