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    In vitro synthesis of polypeptides by optimizing amino acid metabolism
    74.
    发明授权
    In vitro synthesis of polypeptides by optimizing amino acid metabolism 有权
    通过优化氨基酸代谢体外合成多肽

    公开(公告)号：US06994986B2

    公开(公告)日：2006-02-07

    申请号：US09948815

    申请日：2001-09-07

    申请人： James Robert Swartz ,  Dong-Myung Kim

    发明人： James Robert Swartz ,  Dong-Myung Kim

    IPC分类号： C12P21/06

    CPC分类号： C12N9/1033 ,  C07K14/5255 ,  C12P21/00

    摘要： Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides. In order to improve the performance of in vitro protein synthesis reactions, metabolic inhibitors, or manipulation of a source organism, is used to diminish or avoid the action of enzymes responsible for undesirable amino acids production or depletion. A homeostatic system may be used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

    摘要翻译： 提供组合物和方法用于增强多肽的体外合成。 为了改善体外蛋白质合成反应的性能，使用代谢抑制剂或来源生物体的操作来减少或避免负责不良氨基酸产生或消耗的酶的作用。 可以使用稳态系统来生产ATP，其中所需的高能磷酸键是原位生成的。 通过与氧化反应的偶联。 稳态能源本身通常缺乏高能量磷酸键，因此在产生ATP期间将在反应混合物中利用游离磷酸盐。 稳态能量源与催化高能磷酸键的酶的结合，以及能够使用高能磷酸键重新生成ATP的酶组合提供。

    Recombinant animal viral nucleic acids
    78.
    发明授权
    Recombinant animal viral nucleic acids 失效
    重组动物病毒核酸

    公开(公告)号：US06448046B1

    公开(公告)日：2002-09-10

    申请号：US09726651

    申请日：2000-11-28

    申请人： Jon Donson ,  William O. Dawson ,  George L. Grantham ,  Thomas H. Turpen ,  Ann M. Turpen ,  Stephen J. Garger ,  Laurence K. Grill

    发明人： Jon Donson ,  William O. Dawson ,  George L. Grantham ,  Thomas H. Turpen ,  Ann M. Turpen ,  Stephen J. Garger ,  Laurence K. Grill

    IPC分类号： C12N1511

    CPC分类号： C12Y114/18001 ,  C07K14/005 ,  C07K14/415 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/1033 ,  C12N9/1074 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12N15/8203 ,  C12N15/8289 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2830/00 ,  C12N2830/60 ,  C12P41/003 ,  C12Y203/01028 ,  C12Y302/01031 ,  C12Y304/21069

    摘要： The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.

    摘要翻译： 本发明涉及选自具有编码第一病毒亚基因组启动子的天然亚基因启动子的（+）有义单链RNA病毒的重组病毒核酸，编码转录调节的病毒外壳蛋白的核酸序列 通过第一病毒亚基因组启动子，第二病毒亚基因组启动子和其转录受第二病毒亚基因组启动子调控的第二核酸序列。 重组病毒核酸的第一和第二病毒亚基因组启动子相对于彼此不具有同源序列。 重组病毒核酸提供其系统地转录宿主中的第二核酸的特别优点。 本发明包括的宿主生物包括原核生物和真核生物，特别是动物和植物。 本发明还涉及含有感染性病毒载体的病毒，能够产生病毒或其部分的生产细胞，被本发明的病毒感染的宿主，通过表达病毒核酸产生的基因产物和 通过生长受感染的宿主来生产所需产品的方法。