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    Diagnostic methods for neural disorders
    76.
    发明授权
    Diagnostic methods for neural disorders 有权
    神经障碍的诊断方法

    公开(公告)号：US09594075B2

    公开(公告)日：2017-03-14

    申请号：US14692242

    申请日：2015-04-21

    Applicant: Q-STATE BIOSCIENCES, INC.

    Inventor： Kevin C. Eggan ,  Adam Cohen ,  Joel Kralj ,  Evangelos Kiskinis

    IPC: G01N33/50 ,  G01N33/487 ,  G01N33/68 ,  C12N5/0793 ,  G01N21/64

    CPC classification number: G01N33/5091 ,  C12N5/0619 ,  C12N2502/081 ,  C12N2510/00 ,  G01N21/6486 ,  G01N33/48728 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/5058 ,  G01N33/6872 ,  G01N2333/705 ,  G01N2800/2835 ,  G01N2800/2842 ,  G01N2800/302

    Abstract: The invention generally relates to optical methods for the diagnosis of neuronal condition by converting a cell from a patient into a neuron and optically evaluating action potentials of that cell in vitro. The cell is transformed with an optical reporter and exhibits an optical signature in response to neural stimulation. Using genome-editing, a control cell can be made that is isogenic but-for a known mutation and a control signature obtained from the control cell. Thus, methods of the invention reveal potential neurodegenerative effects of a mutation as manifested in a patient's genetic context. The optical signature of the cell, or the difference between the signature and the control signature, is correlated to a diagnosis of the neurodegenerative disease.

    Abstract translation: 本发明一般涉及通过将来自患者的细胞转化为神经元并在体外评估该细胞的动作电位来诊断神经元状况的光学方法。 用光学报告物转化细胞，并响应神经刺激显示光学特征。 使用基因组编辑，可以制备对照细胞，其是同基因的，但是用于已知突变和从对照细胞获得的对照特征。 因此，本发明的方法揭示了突变的潜在的神经退行性作用，其表现在患者的遗传背景中。 细胞的光学特征或者签名与对照特征之间的差异与神经变性疾病的诊断相关。

    COMPOSITION AND METHOD FOR MEASURING THALLIUM INFLUX AND EFFLUX
    77.
    发明申请
    COMPOSITION AND METHOD FOR MEASURING THALLIUM INFLUX AND EFFLUX 审中-公开
    用于测量THILIUM INFLUX和EFFLUX的组合物和方法

    公开(公告)号：US20170067918A1

    公开(公告)日：2017-03-09

    申请号：US15269326

    申请日：2016-09-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel BEACHAM ,  Kyle GEE

    IPC: G01N33/84 ,  G01N33/68

    CPC classification number: G01N33/84 ,  C12Q1/02 ,  G01N33/6872 ,  G01N2333/705

    Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

    Abstract translation: 本发明涉及用于检测细胞中离子通道的活性的方法。 所述方法包括向具有离子通道的电池提供加载缓冲溶液。 加载缓冲液包含至少一种铊指示剂（例如环境敏感的发光染料）和氯离子的生理浓度。 所述方法还包括向细胞提供刺激缓冲液，其中刺激缓冲液包含铊（例如铊离子）。 提供刺激缓冲液使铊通过离子通道流入细胞。 在提供刺激缓冲液之后，检测细胞中染料的发光（例如荧光）。 在存在或不存在铊的情况下，染料的发光可以改变。 该方法可用于测量铊通过离子通道的流入或流出。

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