公开(公告)号：US20190078097A1

公开(公告)日：2019-03-14

申请号：US15772489

申请日：2016-10-28

申请人： Novozymes A/S

发明人： Charles Emrich ,  Jesper Vind

IPC分类号： C12N15/52 ,  C12N15/75 ,  C12N15/80 ,  C12N9/90 ,  C12N9/00 ,  C12N9/88 ,  C12N9/02 ,  C12N9/10 ,  C12N9/40 ,  C12N9/34 ,  C12N9/38 ,  C12N9/48 ,  C12N9/42 ,  C12N9/26

摘要： The present invention relates to polynucleotide constructs for in vitro and in vivo transcription/translation of genes of interest or variants of a gene of interest as well as microorganism host cells comprising such constructs and methods for producing a polypeptide of interest in such microorganism host cells.

公开(公告)号：US20190032105A1

公开(公告)日：2019-01-31

申请号：US16072712

申请日：2017-01-25

申请人： Bio-Rad Europe GmbH ,  Bio-Rad Laboratories, Inc.

发明人： Christophe QUIRING ,  Christine FAVIER ,  Patrice SARFATI ,  Jean Francois MOUSCADET ,  Ronald LEBOFSKY ,  Rebecca DIEVART

IPC分类号： C12Q1/06 ,  C12N9/38 ,  C12Q1/04 ,  C12N9/42 ,  C12N9/40 ,  C12N9/34 ,  C12N9/26 ,  C12N9/48

摘要： Methods, compositions, and kits are provided for rapidly analyzing microbial growth and/or number in a plurality of water-in-oil emulsion droplets.

公开(公告)号：US20190002861A1

公开(公告)日：2019-01-03

申请号：US15934974

申请日：2018-03-24

申请人： PURDUE RESEARCH FOUNDATION

发明人： Philip Stewart Low ,  Venkatesh Chelvam ,  Youngsoon Kim ,  Sumith A. Kularatne

IPC分类号： C12N9/48 ,  A61K49/00 ,  A61K31/454 ,  C12N9/96 ,  A61K51/04 ,  A61K47/54 ,  A61K31/475 ,  A61K31/4745 ,  A61K38/00

CPC分类号： C12N9/485 ,  A61K31/454 ,  A61K31/4745 ,  A61K31/475 ,  A61K38/00 ,  A61K47/542 ,  A61K49/0032 ,  A61K49/0052 ,  A61K51/0402 ,  A61K51/0497 ,  C12N9/96 ,  C12Y304/17021

摘要： Described herein are prostate specific membrane antigen (PSMA) binding conjugates that are useful for delivering therapeutic, diagnostic and imaging agents. Also described herein are pharmaceutical compositions containing them and methods of using the conjugates and compositions. Also described are processes for manufacture of the conjugates and the compositions containing them.

公开(公告)号：US20180364250A1

公开(公告)日：2018-12-20

申请号：US15977727

申请日：2018-05-11

申请人： Colorado State University Research Foundation

发明人： Robert E. Cohen ,  Yun-Seok Choi

IPC分类号： G01N33/68 ,  C07K14/47 ,  C12N9/48 ,  C07K14/435

CPC分类号： G01N33/6842 ,  C07K14/435 ,  C07K14/47 ,  C07K2319/00 ,  C07K2319/70 ,  C12N9/485 ,  C12Y304/19012 ,  G01N33/6872

摘要： The present invention provides compositions comprising chimeric polypeptides that bind to free ubiquitin proteins or free ubiquitin-like proteins with high affinity, as well as chimeric polypeptides that bind to both free and conjugated ubiquitin proteins or free and conjugated ubiquitin-like proteins, and methods of using the chimeric polypeptides to determine the amount of free or toal ubiquitin or free or total ubiquitin-like proteins in various types of samples.

公开(公告)号：US20180328916A1

公开(公告)日：2018-11-15

申请号：US15940962

申请日：2018-03-29

申请人： MONSANTO TECHNOLOGY LLC

发明人： Edward Kraft ,  Renata Bolognesi ,  Artem Evdokimov ,  Farhad Moshiri ,  Meiying Zheng ,  Victor M. Guzov

IPC分类号： G01N33/50 ,  C07K16/28 ,  G01N33/68 ,  C07K16/40 ,  C12N9/16 ,  C12N9/64 ,  C12N9/48 ,  C07K14/705 ,  C07K14/435

CPC分类号： G01N33/5085 ,  C07K14/43563 ,  C07K14/705 ,  C07K16/28 ,  C07K16/40 ,  C12N9/16 ,  C12N9/485 ,  C12N9/6416 ,  G01N33/5014 ,  G01N33/6845 ,  G01N2500/04 ,  G01N2500/10

摘要： The invention relates to identification and characterization of recombinant DNA and polypeptides for specific Bt toxin receptors. In particular, the Bt toxin receptors of the invention include those derived from the Lepidopteran super family including the species Trichoplusiani ni, Pseudoplusia includens, Helicoverpa zea, and Spodoptera frugiperda. The receptors of the invention further include those derived from the Coleopteran super family and particularly from the species Diabrotica virgifera virgifera. The recombinant DNA and polypeptides so provided are useful in the identification and design of novel Bt toxin receptor ligands including novel or improved insecticidal toxins for use in a variety of agricultural applications. Materials and methods for identifying novel toxins are also disclosed herein. The invention also provides methods for selecting toxins to combine to control insect populations by manipulating Bt toxin receptor.

公开(公告)号：US20180236105A1

公开(公告)日：2018-08-23

申请号：US15769931

申请日：2016-10-24

申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION

发明人： Beverly L. Davidson ,  Yong Hong Chen ,  Luis Tecedor

IPC分类号： A61K48/00 ,  C12N9/48 ,  A61K38/13 ,  A61K31/5377 ,  A61K9/00 ,  A61P25/28 ,  C12N7/00

CPC分类号： A61K48/0075 ,  A61K9/0085 ,  A61K31/5377 ,  A61K38/13 ,  A61K38/4813 ,  A61K48/005 ,  A61P25/28 ,  C07K14/015 ,  C12N7/00 ,  C12N9/485 ,  C12N2750/14143 ,  C12N2750/14171 ,  C12Y304/14009 ,  A61K2300/00

摘要： The present disclosure provides methods of treating a lysosomal storage disorder in a mammal which method comprises administering AAV particles encoding a polypeptide directly to the central nervous system of the mammal in conjunction with administering at least two immunosuppressive agents. AAV particles may be delivered by direct injection into the brain, spinal cord, cerebral spinal fluid or a portion thereof.

公开(公告)号：US10047134B2

公开(公告)日：2018-08-14

申请号：US14760123

申请日：2014-01-10

申请人： Biocon Limited

发明人： Sanjay Tiwari ,  Gourav Awasthi ,  Gokul Jothiraman ,  Arun Chandavarkar

IPC分类号： C12N15/81 ,  C12R1/84 ,  C12N15/11 ,  C12N1/16 ,  C07K14/62 ,  C12P21/00 ,  C12N9/48 ,  C12P1/02 ,  A61K38/00

摘要： The present disclosure relates to a comprehensive model for expression of recombinant peptides by Pichia pastoris. The model uses an easily controllable variable called ‘critical nutrient ratio’ for obtaining a right balance between product synthesis and it's degradation during the fermentation process. The extra cellular concentration of precursor could be increased by about 10 folds and the degradation constants could be reduced by about 10-20 folds for intracellular and extracellular cases respectively by controlling critical nutrient ratio and addition of soya flour hydrolysate and EDTA.

公开(公告)号：US09951364B2

公开(公告)日：2018-04-24

申请号：US15021180

申请日：2014-09-10

申请人： NOVOZYMES A/S

发明人： Zhengfang Kang ,  Christie Strahler ,  Suzanne Clark ,  Kristian Bertel Rømer M. Krogh ,  Lan Tang

IPC分类号： C12P7/06 ,  C12P19/00 ,  C12P19/20 ,  C12P19/16 ,  C12P19/14 ,  C12N9/14 ,  C12N9/26 ,  C12N9/28 ,  C12N9/34 ,  C12N9/42 ,  C12N9/48 ,  C12P1/00 ,  C12P21/06 ,  C12N1/00 ,  C12N1/20 ,  C07H21/04 ,  C07K1/00 ,  C12P19/02 ,  C12N9/52 ,  C12P7/14

CPC分类号： C12P19/14 ,  C12N9/2417 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/52 ,  C12P7/06 ,  C12P7/14 ,  C12P19/02 ,  C12P2203/00 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01006 ,  C12Y304/24 ,  Y02E50/17

摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and a thermostable endoglucanase is present and/or added during liquefaction. The invention also relates to compositions suitable for use in processes of the invention.

公开(公告)号：US09896677B2

公开(公告)日：2018-02-20

申请号：US13522548

申请日：2011-01-18

申请人： Jais Rose Bjelke

发明人： Jais Rose Bjelke

IPC分类号： C12N9/48 ,  C12N9/64 ,  C07K14/745

CPC分类号： C12N9/647 ,  C07K14/745 ,  C12N9/644 ,  C12Y304/21022

摘要： The present invention relates to the purification of vitamin K-dependent blood coagulation factors, such as Factor IX (FIX). In particular, the invention provides a method for purifying Factor IX having a desired content of gamma-carboxyglutamic acid from a sample comprising a mixture of species of said Factor IX having different contents of gamma-carboxyglutamic acid, said method comprising the steps of: (a) loading said Factor IX sample onto an immunoaffinity chromatography material coupled to a binding moiety for gamma-carboxyglutamic acid; (b) eluting said Factor IX; and (c) selecting a fraction obtained from said elution wherein the polypeptides in the fraction have the desired content of gamma-carboxyglutamic acids; characterized in that the total concentration of Factor IX within said sample exceeds the binding ability of the immunoaffinity chromatography material.

公开(公告)号：US09611465B2

公开(公告)日：2017-04-04

申请号：US14629452

申请日：2015-02-23

申请人： Celgene Corporation

发明人： Hiroshi Handa ,  Hideki Ando ,  Takumi Itoh ,  Kentaro Hotta

IPC分类号： A61K35/12 ,  C12N15/12 ,  C12N9/00 ,  A61K48/00 ,  C12N9/48 ,  A61K38/00

CPC分类号： C12N9/48 ,  A61K38/00 ,  A61K38/45 ,  C07K14/4705 ,  C07K14/475 ,  C07K19/00 ,  C07K2319/43 ,  C12N9/93 ,  C12Y304/00 ,  C12Y603/02019 ,  G01N33/5058 ,  G01N2500/04 ,  G01N2800/28

摘要： With an aim to provide a novel factor inducing proliferation of neural stem cells and differentiation of these cells into nerve cells, a pharmaceutical composition comprising 1) CRBN, 2) a nucleic acid encoding CRBN, or 3) a stem cell or a neural progenitor cell in which CRBN is expressed, a method including administering the pharmaceutical composition to a non-human animal and inducing proliferation of neural stem cells or neural progenitor cells of the non-human animal and differentiation of these cells into nerve cells, and a method for screening for a therapeutic drug for a disease of cerebral cortex or a surgical injury of cerebral cortex, using CRBN, are provided.